scholarly journals Utilization Of Frozen Goat Semen With Addition Sweet Orange Essential Oil To Improve Genetic Quality in Ujung Teran Village

2019 ◽  
Vol 1 (2) ◽  
pp. 170-174
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa

One of the causes of underdeveloped goats is low genetic quality. The way that can be done to improve the genetic quality of goats is to do cross-breeding with superior goats through Artificial Insemination. The community service aims to increase the knowledge of goat farmers in Ujung Teran Village, Langkat Regency on how to make goat frozen semen using tris yolk extender plus sweet orange essential oil for Artificial Insemination program along with the benefits that can be obtained. The method of service is by giving lectures and training to goat farmers. The results obtained from the implementation of this program are that farmers know how to make frozen semen of goat using sweet orange essential oil on the tris yolk extender and are willing to make Artificial Insemination in their goat cattle.

2019 ◽  
Vol 3 (2) ◽  
pp. 89
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa

One of factors that cause a bad quality of Boer Goat frozen semen is the growth of bacterial. This can be overcome by adding antibiotics such as streptomycin. To further suppress the growth of bacteria can be added other ingredients that contain antibacterials such as sweet orange essential oil. The purpose of this research is to know the percentage value of Membrane Integrity and Acrosome Integrity on Boer Goat frozen semen with addition sweet orange essential oil and streptomycin. The method used was experimental using Completely Randomized Design with 5 treatments and 5 replications. The treatment in this research is addition 0%, 0,25%; 0.5%; 0.75% and 1% sweet orange essential oil on tris yolk and streptomycin extender. The results showed the best treatment addition combination streptomycin and sweet orange essential oil to percentage Membrane Integrity and Acrosome Integrity is increase 1% sweet orange essential oil.Keywords: Boer Goat, essential oil, frozen semen, streptomycin, sweet orange.


Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa

Aims: This study aimed to determine the quality of Simmental Bull liquid semen through laboratory tests, including the percentage value of membrane integrity Spermatozoa with the addition of gentamycin and sweet orange essential oil to tris yolk extender. Methodology: The material used in this research is Simmental Cattle bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender was prepared with Tris (hydroxymethyl aminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquades (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replicates. The treatment given is the addition of sweet orange essential oil 0%, 0.25%, 0.5%, 0.75% and 1%. The parameter observed was the evaluation of membrane integrity before equilibration and after equilibration. Results: The results showed that the percentage of membrane integrity in Simmental bull liquid semen continues to increase with the addition of sweet orange essential oils. The results showed that the addition of sweet orange essential oil as much as 0% produced a membrane integrity percentage after equilibration of 63%, adding 0.25% resulted in 68%, adding 0.5% yielded 70%, adding 0.75% yielded 75% and adding 1% resulted in 79%. Conclusion: The best percentage of membrane integrity in Simmental bull liquid semen was the addition of gentamicin and sweet orange essential oil in the tris yolk extender at the level of 1%.


Author(s):  
Sukma Aditya Sitepu ◽  
Zaituni Udin ◽  
. Jaswandi ◽  
. Hendri

Aims: To determine the effect of the addition of sweet orange essential oil to the extender (tris yolk extender without the addition of antibiotics/antibacterial) to total bacteria Boer Buck frozen semen. Study Design: Randomized Block Design. Place and Duration of Study: Sample: Laboratory Reproduction of Loka Penelitian Kambing Potong Sei Putih Indonesia, between January and March 2019. Methodology: The research procedure starts with the preparation of semen extender, collection of fresh semen, dilution of semen, equilibration, freezing of semen, and thawing. This research obtained conducted using a Randomized Block Design consisting of 5 treatment levels and five replications. Semen storage using 3 Boer buck, which done for three days. As a treatment is the addition of sweet orange essential oil as much as (P0) 0%, (P1) 0,25%, (P2) 0,5%, (P3) 0,75% and (P4) 1% on the tris yolk extender. The observed variables was total bacteria evaluated before freezing and after freezing (Post-Thawing). Results: The results showed that the addition of sweet orange essential oil had a very significant effect (P <0.01). The results of adding sweet orange essential oil to the extender (Post-Thawing) were 78.6 x 102 CFU (P0), 76 x 102 CFU (P1), 73,2 x 102 CFU (P2), 71 x 102 CFU (P3) and 68.6 x 102 CFU (P4). Conclusion: The best values the total bacteria 68.6 x 102 CFU (P4). It can conclude that the best result of adding sweet orange essential oil to tris yolk extender is (P4) 1%.


Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa

Aims: The purpose of this study was to determine the percentage value of spermatozoa acrosome integrity with the addition of gentamicin and sweet orange essential oil on the tris yolk extender on Simmental bull liquid semen. Methodology: The material that was used in this research included Simmental bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender prepares with Tris (hydroxymethyl) aminomethane (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aqua dest (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replications. The treatments comprised the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1% inclusion levels. The parameter observed was the evaluation of the percentage value of acrosome integrity before and after equilibration equilibration. Results: The results showed that the percentage value of acrosome integrity in spermatozoa linearly increased along with the addition of sweet orange essential oil. The addition of sweet orange essential oil had a very significant effect (P <0.01) on the percentage of spermatozoa acrosome integrity in Simmental bull liquid semen. The percentage of acrosome integrity on the addition of 0% (P0) sweet orange essential oil is 60%, the addition of 0.25% (P1) is 64%,the addition of 0.5% (P2) is 67%, the addition of 0.75% (P3) is 69%, and the addition of 1% (P4) is 73%. Conclusion: The addition of 1% (P4) sweet orange essential oil can increase the value of acrosome integrity on spermatozoa, which subsequently improve the quality of Simmental bull liquid semen.


Author(s):  
Sukma Aditya Sitepu

This research aims to to determine the percentage value of spermatozoa abnormalities in Boer Goat frozen semen by adding a combination of streptomycin with sweet orange essential oil to tris yolk extender. The ingredients used in this study were fresh semen from Boer Goat, tris yolk extender, streptomycin, and sweet orange essential oil. Tris yolk extender is made using Tris (hydroxymethylaminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquabides (100 ml). The study design was a non factorial completely treatments given were the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1%. The results showed that the addition of a combination of streptomycin and sweet orange essential oil into the tris yolk extender had a highly significant effect (P <0.01) on the percentage value of spermatozoa abnormalities of Boer Goats before and after freezing. The best average value of spermatozoa abnormalities is the addition of sweet orange essential oil at 1% (P4) with the percentage of spermatozoa abnormalities before freezing at 3% and after freezing at 6%.


2018 ◽  
Vol 1 (2) ◽  
pp. 78 ◽  
Author(s):  
Sukma Aditya Sitepu ◽  
Zaituni Udin ◽  
Jaswandi Jaswandi ◽  
Hendri Hendri

AbstractThe purpose of this study was to know the quality of Boer liquid semen during storage by adding sweet orange essential oil and gentamicin into the tris yolkextender.The semenlongevity test was carried out by storing semen in a closed tube at room temperature and refrigerator, and evaluated motility and Viability every 3 hours at room temperature and 12 hours in refrigerator. The results showed that the characteristics of the liquid semen, with the addition of 1% of essential oils showed percentage of motility and Viability significantly higher than 0.5% and without the addition of essential oil, either stored at room temperature or in the refrigerator. This may be related to the content of essential oils of sweet orange peel containing flavonoids and antibacterials that are capable of maintaining liquid semen quality of Boer Goat.In addition, gentamicin contains antibacterials capable of suppressing the growth of bacteria which can damage and durabilitythe spermatozoa.Keywords: Boer Goat,Gentamicin, Liquid semen, Sweet orange


Bakti Budaya ◽  
2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Siti Aminah ◽  
Uswatun Hasanah ◽  
Imam Wicaksono

The Covid-19 pandemic has brought a new life order which is commonly known as a health protocol implementation. Efforts to deal with the Covid-19 pandemic can be made physically or spiritually. Physical efforts are carried out by applying health protocols in a disciplined manner, while spiritual efforts are carried out by increasing the quality of worship of God. These two efforts must be combined with maintaining the quality of worship in health protocols. In this case, the quality of worship can be improved by deepening the understanding and application of Arabic tajwid and grammar in reciting verse and prayer recitations. In addition, it is necessary to practice morning and evening prayers as self-protection and maintain mental stability. To achieve this goal, this team carried out a community service by compiling two pocketbooks. The first one is related to the application of health protocols in daily worship and another pocketbook containing tajwid material and morning prayer, which was equipped with exposure and training on recitation and reading the morning prayer and evening prayers. These exposures are presented in outreach videos and tutorials. The result shows that people are increasingly aware of implementing health protocols in their daily activities, especially in Islamic worship. In addition, the community's mentality has become more robust, and people have become calmer in living their lives during the pandemic since some people have practiced morning and evening prayers. In addition, they also apply tajwid and Arabic grammar in a better way so that their worship activities become much more solemn. ===== Pandemi Covid-19 membawa manusia kepada tatanan kehidupan baru dengan menerapkan protokol kesehatan. Salah satu hal yang dapat dilakukan dalam mengahadapi pandemi Covid-19 dan terhindar dari penularan virus dapat dilakukan secara jasmani maupu rohani. Upaya jasmaniah dilakukan dengan menerapkan protokol kesehatan secara disiplin, sedangkan upaya rohani dilakukan dengan mendekatkan diri kepada Tuhan dengan meningkatkan kualitas peribadatan. Hal tersebut dapat dilakukan dengan tetap melakukan ibadah dengan menerapkan protokol kesehatan, diikuti dengan pemahaman dan penerapan tajwid dan gramatika Arab dengan benar dalam melafalkan bacaan ayat dan doa. Selain itu, diperlukan pula pengamalan doa pagi dan petang untuk menjaga diri dan meningkatkan kesehatan mental. Untuk mencapai tujuan tersebut, tim  melaksanakan pengabdian dengan menyusun buku saku penerapan protokol kesehatan dalam peribadatan sehari-hari dan buku saku yang berisi materi tajwid dan doa pagi-petang yang diikuti dengan penyuluhan dan pelatihan penerapan tajwid dan membaca doa pagi dan petang yang dilakukan dengan membuat video penyuluhan dan tutorial. Hal ini menunjukkan hasil bahwa masyarakat semakin sadar untuk menerapkan protokol kesehatan dalam setiap aktivitas sehari-hari, khususnya dalam peribadatan Islam. Selain itu, mental masyarakat menjadi semakin kuat dan masyarakat menjadi lebih tenang dalam menjalani kehidupan selama pandemi. Hal ini karena sebagian masyarakat telah mengamalkan doa pagi dan petang, serta menerapkan tajwid dan gramatika Arab dengan lebih baik sehingga aktivitas ibadah mereka menjadi semakin khusyu’.


2020 ◽  
Vol 143 ◽  
pp. 991-999 ◽  
Author(s):  
Jayuri Susy Fernandes de Araújo ◽  
Evandro Leite de Souza ◽  
Jéssica Ribeiro Oliveira ◽  
Ana Cristina Alves Gomes ◽  
Lloyd Ryan Viana Kotzebue ◽  
...  

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