scholarly journals COMBINED EFFECT OF STREPTOMYCIN AND SWEET ORANGE ESSENTIAL OIL TO MEMBRANE AND ACROSOME INTEGRITY BOER GOAT FROZEN SEMEN

2019 ◽  
Vol 3 (2) ◽  
pp. 89
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Boer Goat ◽  
Randomized Design ◽  
Frozen Semen ◽  
Acrosome Integrity ◽  
Completely Randomized Design ◽  
Orange Essential Oil

One of factors that cause a bad quality of Boer Goat frozen semen is the growth of bacterial. This can be overcome by adding antibiotics such as streptomycin. To further suppress the growth of bacteria can be added other ingredients that contain antibacterials such as sweet orange essential oil. The purpose of this research is to know the percentage value of Membrane Integrity and Acrosome Integrity on Boer Goat frozen semen with addition sweet orange essential oil and streptomycin. The method used was experimental using Completely Randomized Design with 5 treatments and 5 replications. The treatment in this research is addition 0%, 0,25%; 0.5%; 0.75% and 1% sweet orange essential oil on tris yolk and streptomycin extender. The results showed the best treatment addition combination streptomycin and sweet orange essential oil to percentage Membrane Integrity and Acrosome Integrity is increase 1% sweet orange essential oil.Keywords: Boer Goat, essential oil, frozen semen, streptomycin, sweet orange.

scholarly journals Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender

2020 ◽  
pp. 42-46
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Integrity Test ◽  
Randomized Design ◽  
Simmental Cattle ◽  
Fresh Semen ◽  
Orange Essential Oil

Aims: This study aimed to determine the quality of Simmental Bull liquid semen through laboratory tests, including the percentage value of membrane integrity Spermatozoa with the addition of gentamycin and sweet orange essential oil to tris yolk extender. Methodology: The material used in this research is Simmental Cattle bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender was prepared with Tris (hydroxymethyl aminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquades (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replicates. The treatment given is the addition of sweet orange essential oil 0%, 0.25%, 0.5%, 0.75% and 1%. The parameter observed was the evaluation of membrane integrity before equilibration and after equilibration. Results: The results showed that the percentage of membrane integrity in Simmental bull liquid semen continues to increase with the addition of sweet orange essential oils. The results showed that the addition of sweet orange essential oil as much as 0% produced a membrane integrity percentage after equilibration of 63%, adding 0.25% resulted in 68%, adding 0.5% yielded 70%, adding 0.75% yielded 75% and adding 1% resulted in 79%. Conclusion: The best percentage of membrane integrity in Simmental bull liquid semen was the addition of gentamicin and sweet orange essential oil in the tris yolk extender at the level of 1%.

scholarly journals Acrosome Integrity Spermatozoa Test with Addition of Gentamicin and Sweet Orange Essential Oil in Simmental Bull Liquid Semen

2020 ◽  
pp. 1-5
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Citric Acid ◽  
Essential Oil ◽  
Sweet Orange ◽  
Egg Yolk ◽  
Randomized Design ◽  
Before And After ◽  
Acrosome Integrity ◽  
Fresh Semen ◽  
Orange Essential Oil

Aims: The purpose of this study was to determine the percentage value of spermatozoa acrosome integrity with the addition of gentamicin and sweet orange essential oil on the tris yolk extender on Simmental bull liquid semen. Methodology: The material that was used in this research included Simmental bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender prepares with Tris (hydroxymethyl) aminomethane (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aqua dest (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replications. The treatments comprised the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1% inclusion levels. The parameter observed was the evaluation of the percentage value of acrosome integrity before and after equilibration equilibration. Results: The results showed that the percentage value of acrosome integrity in spermatozoa linearly increased along with the addition of sweet orange essential oil. The addition of sweet orange essential oil had a very significant effect (P <0.01) on the percentage of spermatozoa acrosome integrity in Simmental bull liquid semen. The percentage of acrosome integrity on the addition of 0% (P0) sweet orange essential oil is 60%, the addition of 0.25% (P1) is 64%,the addition of 0.5% (P2) is 67%, the addition of 0.75% (P3) is 69%, and the addition of 1% (P4) is 73%. Conclusion: The addition of 1% (P4) sweet orange essential oil can increase the value of acrosome integrity on spermatozoa, which subsequently improve the quality of Simmental bull liquid semen.

scholarly journals Utilization Of Frozen Goat Semen With Addition Sweet Orange Essential Oil To Improve Genetic Quality in Ujung Teran Village

2019 ◽  
Vol 1 (2) ◽  
pp. 170-174
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Community Service ◽  
Artificial Insemination ◽  
Sweet Orange ◽  
Genetic Quality ◽  
Know How ◽  
Frozen Semen ◽  
And Training ◽  
Orange Essential Oil

One of the causes of underdeveloped goats is low genetic quality. The way that can be done to improve the genetic quality of goats is to do cross-breeding with superior goats through Artificial Insemination. The community service aims to increase the knowledge of goat farmers in Ujung Teran Village, Langkat Regency on how to make goat frozen semen using tris yolk extender plus sweet orange essential oil for Artificial Insemination program along with the benefits that can be obtained. The method of service is by giving lectures and training to goat farmers. The results obtained from the implementation of this program are that farmers know how to make frozen semen of goat using sweet orange essential oil on the tris yolk extender and are willing to make Artificial Insemination in their goat cattle.

scholarly journals Spermatozoa Abnormality Test of Boer Goats Frozen Semen with the Addition of Sweet Orange Essential Oil and Streptomycin in Tris Yolk Extender

2020 ◽  
pp. 20-23
Author(s):  
Sukma Aditya Sitepu
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Egg Yolk ◽  
Boer Goat ◽  
Average Value ◽  
Frozen Semen ◽  
Before And After ◽  
Boer Goats ◽  
Fresh Semen ◽  
Orange Essential Oil

This research aims to to determine the percentage value of spermatozoa abnormalities in Boer Goat frozen semen by adding a combination of streptomycin with sweet orange essential oil to tris yolk extender. The ingredients used in this study were fresh semen from Boer Goat, tris yolk extender, streptomycin, and sweet orange essential oil. Tris yolk extender is made using Tris (hydroxymethylaminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquabides (100 ml). The study design was a non factorial completely treatments given were the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1%. The results showed that the addition of a combination of streptomycin and sweet orange essential oil into the tris yolk extender had a highly significant effect (P <0.01) on the percentage value of spermatozoa abnormalities of Boer Goats before and after freezing. The best average value of spermatozoa abnormalities is the addition of sweet orange essential oil at 1% (P4) with the percentage of spermatozoa abnormalities before freezing at 3% and after freezing at 6%.

scholarly journals QUALITY DIFFERENCES OF BOER LIQUID SEMEN DURING STORAGE WITH ADDITION SWEETORANGEESSENTIAL OIL IN TRIS YOLK AND GENTAMICIN EXTENDER

2018 ◽  
Vol 1 (2) ◽  
pp. 78 ◽  
Author(s):  
Sukma Aditya Sitepu ◽  
Zaituni Udin ◽  
Jaswandi Jaswandi ◽  
Hendri Hendri
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Room Temperature ◽  
Semen Quality ◽  
Sweet Orange ◽  
Orange Peel ◽  
Boer Goat ◽  
Closed Tube ◽  
Growth Of Bacteria

AbstractThe purpose of this study was to know the quality of Boer liquid semen during storage by adding sweet orange essential oil and gentamicin into the tris yolkextender.The semenlongevity test was carried out by storing semen in a closed tube at room temperature and refrigerator, and evaluated motility and Viability every 3 hours at room temperature and 12 hours in refrigerator. The results showed that the characteristics of the liquid semen, with the addition of 1% of essential oils showed percentage of motility and Viability significantly higher than 0.5% and without the addition of essential oil, either stored at room temperature or in the refrigerator. This may be related to the content of essential oils of sweet orange peel containing flavonoids and antibacterials that are capable of maintaining liquid semen quality of Boer Goat.In addition, gentamicin contains antibacterials capable of suppressing the growth of bacteria which can damage and durabilitythe spermatozoa.Keywords: Boer Goat,Gentamicin, Liquid semen, Sweet orange

scholarly journals THE PROPORTION OF X AND Y SPERM, VIABILITY AND MOTILITY OF RAM SPERMATOZOA AFTER SEPARATED WITH WHITE EGG ALBUMIN

2017 ◽  
Vol 41 (1) ◽  
pp. 1 ◽  
Author(s):  
Moh Takdir ◽  
Ismaya Ismaya ◽  
Sigit Bintara
Keyword(s):  
Egg White ◽  
Egg Albumin ◽  
Randomized Design ◽  
Frozen Semen ◽  
Bottom Fraction ◽  
Completely Randomized Design ◽  
Ram Sperm ◽  
Artificial Vagina ◽  
Range Test

ABSTRACT The aim of this research was to determine the proportion, viability and motility of X and Y ram spermatozoa separated with egg white albumin. Sperm samples derived from Garut ram, which was collected by using an artificial vagina. Observations were made on spermatozoa fraction above and below each medium fraction treatment. There are treatment egg white albumin as separation medium, each medium consisting of fractions top and bottom fraction with different concentration: 1) P0 = sperma before separation (control); 2) P1 = 10% above fraction + 30% lower fraction; P2 = 25% + 45%; P3 = 25% + 75%. Data proportion of X and Y, viability and motility were analyzed statistically by Completely Randomized Design patern in the direction followed by Duncan’s Multiple Range Test for data with a real difference. Separation with egg white albumin affect significantly increased the proportion of spermatozoa X and Y (P≤0.05), but tends to decrease the viability and motility of spermatozoa.The proportion of spermatozoa X and Y was highest in treatment P3,76.76% of spermatozoa X (fraction above 25%) and 79.81% spermatozoa Y (75% lower fraction), with an average viability obtained respectively 68,9% (fraction above) and 59,7% (bottom fraction), motility 77,5% (fraction above) dan 84,0% (bottom fraction). It was concluded that the egg white albumin is very effective in changing the proportions of X and Y ram sperm with the quality of spermatozoa after separation feasible for applications insemination or processed into frozen semen.  (Keywords: Garut ram, White egg albumin, Spermatozoa X and Y) 

Pengaruh Temperatur dan Lama Thawing terhadap Kualitas Spermatozoa Sapi dalam Penyimpanan Straw Beku

2010 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
S Utomo ◽  
E Boquifai
Keyword(s):  
Key Words ◽  
The Other ◽  
Effect Of Temperature ◽  
Randomized Design ◽  
Frozen Semen ◽  
Completely Randomized Design ◽  
Motility Of Sperm ◽  
Better Than

<p>This research was conducted to investigate the effect of temperature and duration of<br />thawing frozen semen in ministraw on quality sperm. Seventy five ministraws frozen semen of Simmental breed were thawing by water with temperature 5, 26 and 37 °C, with duration of 5, 10, 15, 20 and 30 second. The quality sperm post thawing was measured based on pH and motility. The design of this research was completely randomized design with factorial clasification 3 x 5. The result showed that the motility of sperm was thawing 37°C better than the other temperature. The quality of sperm was no affected by temperature and duration of thawing. It could be concluded<br />that thawing of frozen semen will have better done at 37°C with duration of 15 second.</p><p>Key words : Thawing, sperm, ministraw, frozen semen.</p>

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