The growth, production and chemical compounds of Chlorella sp. in various concentrations of palm oil wastewater in laboratory culture media

AbstractMedicinal plants are considered as one of the most important sources of chemical compounds, so preparing a suitable culture media for medicinal plant growth is a critical factor. The present study is aimed to improve the caffeic acid derivatives and alkylamides percentages of Echinacea purpurea root extract in hydroponic culture media with different perlite particle size and NO3−/NH4+ ratios. Perlite particle size in the growing media was varied as very coarse perlite (more than 2 mm), coarse perlite (1.5–2 mm), medium perlite (1–1.5 mm), fine perlite (0.5–1 mm), and very fine perlite (less than 0.5 mm) in different ratios to peat moss (including pure perlite, 50:50 v/v, 30:70 v/v, and pure peat moss). Two NO3−/NH4+ ratios (90:10 and 70:30) were tested in each growing media. All phytochemical analyses were performed according to standard methods using high performance liquid chromatography (HPLC). It was found that the E. purpurea grown in the medium containing very fine-grade perlite with 50:50 v/v perlite to peat moss ratio had the maximum caffeic acid derivatives, including chicoric acid (17 mg g−1 DW), caftaric acid (6.3 mg g−1 DW), chlorogenic acid (0.93 mg g−1 DW), cynarin (0.84 mg g−1 DW), and echinacoside (0.73 mg g−1 DW), as well as, alkylamides (54.21%). The percentages of these phytochemical compounds increased by decreasing perlite particle size and increasing of NO3−/NH4+ ratio. The major alkylamide in the E. purpurea root extract was dodeca-2E, 4E, 8Z-10 (E/Z)-tetraenoic acid isobutylamide in all treatments, ranging from 31.12 to 54.21% of total dry weight. It can be concluded that optimizing hydroponic culture media and nutrient solution has significant effects on E. purpurea chemical compounds.

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Synthesis and application of polyacrylamide grafted magnetic cellulose flocculant for palm oil wastewater treatment

Journal of Environmental Chemical Engineering ◽

10.1016/j.jece.2020.104014 ◽

2020 ◽

Vol 8 (4) ◽

pp. 104014 ◽

Cited By ~ 1

Author(s):

Mohamed Hizam Mohamed Noor ◽

Norzita Ngadi ◽

Ibrahim Mohammed Inuwa ◽

Lawal Anako Opotu ◽

Mohd Ghazali Mohd Nawawi

Keyword(s):

Wastewater Treatment ◽

Palm Oil ◽

Oil Wastewater

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IDENTIFIKASI LIMBAH PALM OIL MILL EFFLUENT (POME) MENGGUNAKAN BIOSENSOR BERBASIS ALGA

JOURNAL ONLINE OF PHYSICS ◽

10.22437/jop.v7i1.14387 ◽

2021 ◽

Vol 7 (1) ◽

pp. 1-6

Author(s):

Dina Maryani ◽

Lazuardi Umar

Keyword(s):

Chemical Oxygen Demand ◽

Palm Oil ◽

Biochemical Oxygen Demand ◽

Oxygen Demand ◽

Palm Oil Mill Effluent ◽

Chlorella Sp ◽

Palm Oil Mill

Palm Oil Mill Effluent (POME) merupakan limbah minyak kelapa sawit yang memiliki kadar Chemical Oxygen Demand (COD) dan Biochemical Oxygen Demand (BOD) yang tinggi dan sehingga merusak ekosistem periairan serta menurunkan kadar oksigen terlarut (DO). Namun, POME memiliki nutrisi untuk sel alga dalam memproduksi oksigen. Berdasarkan hal tersebut diperlukan identifikasi POME yang jatuh kebadan air berdasarkan fotosintesis Chlorella sp. menggunakan biosensor. Biosensor telah banyak dikembangkan dalam aplikasi bidang lingkungan dengan melihat kadar oksigen terlarut sebagai kualitas perairan. Penelitian ini menggunakan biosensor dengan prinsip sensor amperometris tipe Biochip-G. Sensor amperometris mengukur perubahan arus dari reaksi reduksi dan oksidasi dan menghasilkan potensial keluaran yang terukur. Potensial keluaran yang terukur merupakan kadar oksigen terlarut dari penambahan POME terhadap Chlorella sp. ketika proses fotosintesis terjadi. Identifikasi POME berdasarkan proses fotosintesis Chlorella sp. menggunakan cahaya artifisial LED Putih 380 nm-780 nm dengan tingkat variasi konsentrasi POME sebanyak 10%, 20%, dan 30%. Berdasarkan pengukuran kadar oksigen terlarut (DO) menghasilkan nilai DO sebesar 174.15%, 154.66%, dan 138.98% serta nilai sensitivitas sebesar 4mV/%POME.

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Reaction of Opalinas to Various Laboratory Culture Media

Transactions of the American Microscopical Society ◽

10.2307/3222360 ◽

1928 ◽

Vol 47 (1) ◽

pp. 1 ◽

Cited By ~ 3

Author(s):

Mary E. Larson

Keyword(s):

Culture Media ◽

Laboratory Culture

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Rapid sequencing of MRSA direct from clinical plates in a routine microbiology laboratory

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkz170 ◽

2019 ◽

Vol 74 (8) ◽

pp. 2153-2156 ◽

Cited By ~ 5

Author(s):

Beth Blane ◽

Kathy E Raven ◽

Danielle Leek ◽

Nicholas Brown ◽

Julian Parkhill ◽

...

Keyword(s):

Dna Extraction ◽

Clinical Laboratory ◽

Culture Media ◽

Direct Sequencing ◽

Laboratory Culture ◽

Outbreak Detection ◽

Snp Analysis ◽

University Hospitals ◽

Quality Control Metrics ◽

Clinical Culture

Abstract Background Routine sequencing of MRSA could bring about significant improvements to outbreak detection and investigation. Sequencing is commonly performed using DNA extracted from a pure culture, but overcoming the delay associated with this step could reduce the time to infection control interventions. Objectives To develop and evaluate rapid sequencing of MRSA using primary clinical cultures. Methods Patients with samples submitted to the clinical laboratory at the Cambridge University Hospitals NHS Foundation Trust from which MRSA was isolated were identified, the routine laboratory culture plates obtained and DNA extraction and sequencing performed. Results An evaluation of routine MRSA cultures from 30 patients demonstrated that direct sequencing from bacterial colonies picked from four different culture media was feasible. The 30 clinical MRSA isolates were sequenced on the day of plate retrieval over five runs and passed quality control metrics for sequencing depth and coverage. The maximum contamination detected using Kraken was 1.09% fragments, which were identified as Prevotella dentalis. The most common contaminants were other staphylococcal species (25 isolate sequences) and Burkholderia dolosa (11 isolate sequences). Core genome pairwise SNP analysis to identify clusters based on isolates that were ≤50 SNPs different was used to triage cases for further investigation. This identified three clusters, but more detailed genomic and epidemiological evaluation excluded an acute outbreak. Conclusions Rapid sequencing of MRSA from clinical culture plates is feasible and reduces the delay associated with purity culture prior to DNA extraction.

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MICROALGAE FOR CO2 REDUCTION AND WASTEWATER TREATMENT APPLICATION IN INDUSTRIAL AREA

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.28930/jitkt.v3i2.7822 ◽

2011 ◽

Vol 3 (2) ◽

Author(s):

Arif Dwi Santoso ◽

Rahmania A. Darmawan ◽

Joko P. Susanto

Keyword(s):

Waste Water ◽

Water Treatment ◽

Culture Media ◽

Waste Water Treatment ◽

Treatment Plant ◽

Co2 Concentration ◽

Water Treatment Plant ◽

Industrial Area ◽

Waste Water Treatment Plant ◽

Chlorella Sp

<p>The microalgae incorporated photobioreactor (PBR) is a highly efficient biological system for converting carbon dioxide (CO2) gases into biomass and treating wastewater. In this study, the freshwater microalgae Chlorella sp. was cultured in two unit photobioreactors which were connected to a boiler of dairy factory. The source of water for microalgae culture media (in the photobioreactors) are collected from the waste water treatment plant of the dairy factory itself. The objective of this microalgae photobioreactor activity were to reduce CO2, produce biomass and as a part of a waste water treatment in a dairy industry in Indonesia. Both photobioreactors were operated for 14 days culture interval in the semi continuous cultivation, with initial stocking rate of 2 x 105 cells/ml microalgae. Carbon dioxide concentration from the boiler stack was 10-11 % vol. The photobioreactors were injected with the specified CO2 concentration with flow rate of 2 l/min. and 1.5 l/min. The result showed that microalgae photobioreactors capability in CO2 absorption were 0.78 ± 0.25 and 0.92 ±0.36 g CO2/l media/day, respectively. Before and after utilized by the photobioreactors system, Nitrate and Phosphate concentration of the culture media (supplied from the waste water treatment plant) decreased from 3-4 mg/l to 0.05-0.1 mg/l. After 14 days cultivation, biomass concentration of microalgae which were injected with CO2 flow rate of 2 l/min. and 1.5 l/min. were 19 x 106 cells/ml and 15 x 106 cells/ml respectively. Results showed that microalgal photobioreactors operated in an industrial area could reduce CO2 concentration and produce biomass.</p><p>Keywords: photobioreactor (PBR), Chlorella sp., CO2 flue gas emissions, wastewater</p>

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Productivity and quality of Moina sp. cultivated with various culture medium

Jurnal Akuakultur Indonesia ◽

10.19027/jai.20.2.148-162 ◽

2021 ◽

Vol 20 (2) ◽

pp. 148-162

Author(s):

Taufik Shidik Adi Nugroho Shidik ◽

Julie Ekasari ◽

Dedi Jusadi ◽

Mia Setiawati

Keyword(s):

Fatty Acids ◽

Organic Material ◽

Culture Medium ◽

Unsaturated Fatty Acids ◽

Protein Quality ◽

Culture Media ◽

Mass Scale ◽

Chlorella Sp ◽

Study Results

Cultivation of Moina sp is still constrained by its quality, productivity, and sustainability. The alternative solution is the use of cultivation media materials that have high nutritional content and easily available in large quantities to support the quality and productivity of Moina sp. and meet the needs of live feed. The objective of the study was to evaluate the effect of various culture medium on the productivity and nutritional quality of Moina sp.. Five culture media were tested in laboratory scale, i.e. organic ingredient (BO), Chlorella sp. (Ch), Chlorella sp. + organic ingredients (ChBO), biofloc (BF) and biofloc + organic ingredients (BFBO). While in mass scale, four culture media were tested, i.e. Chlorella sp. (Ch), Chlorella sp. + Organic Ingredients (ChBO), Biofloc (BF) and Biofloc + Organic Ingredients (BFBO). The peaks of Moina sp. density in different treatments were achieved in different days. ChBO treatments significantly had higher productivity (P<0.05). The highest protein content was found in Moina sp. cultured with ChBO media, even higher than artemia. Moina sp. cultured with Chlorella sp. (Ch) showed the highest PUFA (Poly Unsaturated Fatty Acids) contents, while the highest MUFA (mono unsaturated fatty acids) contents was obtained from Moina sp. cultured with BFBO media lower than artemia. The study results indicates that different culture media produces different productivity and nutrient quality of Moina sp. The organic material combination of Chlorella sp. + organic material (ChBO) was the best media to improve the productivity and protein quality of Moina sp. Keywords : Biofloc, Chlorella sp., Moina sp., organic matter, productivity, quality ABSTRAK Budidaya Moina sp. masih terkendala pada kualitas, produktivitas dan kestabilan dalam ketersediaannya. Untuk itu diperlukan penggunaan bahan media budidaya yang memiliki kandungan nutrisi tinggi dan mudah didapat dalam jumlah banyak untuk mendukung kualitas dan produktivitas Moina sp. demi memenuhi kebutuhan pakan hidup. Tujuan penelitian yaitu mengevaluasi pengaruh berbagai media budidaya terhadap produktivitas dan kualitas nutrisi Moina sp. Lima media kultur yang diuji dalam penelitian laboratorium yaitu Bahan Organik (BO), Chlorella sp. (Ch), Chlorella sp. + Bahan Organik (ChBO), Bioflok (BF) dan Bioflok + Bahan Organik (BFBO). Sedangkan pada penelitian skala massal diuji empat media kultur yaitu Chlorella sp. (Ch), Chlorella sp. + Bahan Organik (ChBO), Bioflok (BF) dan Bioflok + Bahan Organik (BFBO). Puncak kepadatan Moina sp. pada tiap perlakuan dicapai pada hari yang berbeda. Perlakuan ChBO memiliki produktivitas yang lebih tinggi (P<0,05). Kandungan protein Moina sp. tertinggi ditemukan pada media ChBO dan bahkan lebih tinggi dari pada artemia. Moina sp. yang dibudidayakan dengan Chlorella sp. (Ch), menunjukkan kandungan PUFA tertinggi, sedangkan kandungan MUFA yang tertinggi terdapat pada Moina sp. yang dibudidayakan dengan bahan media BFBO namun masih lebih rendah jika dibandingkan dengan kandungan pada artemia. Hasil penelitian menunjukkan media kultur yang berbeda menghasilkan produktivitas dan kualitas nutrisi moina yang berbeda. Kombinasi bahan organik Chlorella + bahan organik (ChBO) merupakan media terbaik dibandingkan dengan perlakuan lainnya untuk meningkatkan produktivitas dan kualitas nutrisi terutama protein Moina sp. Kata kunci : Bioflok, Chlorella sp., Moina sp., bahan organik, produktivitas, kualitas

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Culturing of green photosynthetic microalgae (Chlorella sp.) using palm oil mill effluent (POME) for future biodiesel production

Maejo International Journal of Energy and Environmental Communication ◽

10.54279/mijeec.v2i1.244945 ◽

2020 ◽

Vol 2 (1) ◽

pp. 1-8

Author(s):

Nithin B. R. ◽

Prakash Bhuyar ◽

Marlen Trejo ◽

Mohd Hasbi Ab. Rahim ◽

Gaanty Pragas Maniam ◽

...

Keyword(s):

Water Pollution ◽

Growth Rate ◽

Green Algae ◽

Palm Oil ◽

Oxygen Demand ◽

Total Cell ◽

Palm Oil Mill Effluent ◽

Total Cell Count ◽

Chlorella Sp ◽

Palm Oil Mill

Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from palm oil production is a big problem to Malaysia which is largest palm oil producers. POME containing large amount of chemical oxygen demand (COD) which can cause severe pollution to the environment especially water. This present study designed for culturing green algae (Chlorella sp.) isolated from Pantai Balok, Kuantan which is most common microalgae with palm oil mill effluent (POME). Chlorella sp. was cultured using BG-11 medium with the addition of POME as experiment and without POME as control. The POME responsible for the growth of green algae is studied by measurement of the growth rate, total cell count and chemical oxygen demand (COD) for both conditions. The composition for control and experiment is also measured and determined by using Gas Chromatography Mass Spectrometry (GCMS). From the results, it is observed that the total cell count and growth rate of Chlorella sp. greater in presence of POME since the green microalgae absorbs the essential nutrients from the POME as their nutrients. GCMS revealed that the difference in composition for both the conditions. Results concluded that several COD also vigorously deplete with the help of green algae digestion. Cultivation of Chlorella sp. in POME will help in future water pollution treatment.

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Analisis Kualitas Air dan Status Mutu Air Sungai Batang Asam Akibat Limbah Cair Kelapa Sawit Menggunakan Metode Indeks Pencemaran

Jurnal Daur Lingkungan ◽

10.33087/daurling.v1i2.9 ◽

2018 ◽

Vol 1 (2) ◽

pp. 41

Author(s):

Siti Umi Kalsum ◽

Lailal Gusri ◽

Junardi Junardi

Keyword(s):

Water Quality ◽

Palm Oil ◽

Pollution Index ◽

Class Ii ◽

Quality Standard ◽

Water Supply Systems ◽

Index Method ◽

Oil Companies ◽

The Status ◽

Oil Wastewater

Batang Asam River as a source of raw water for Drinking Water Supply Systems (SPAM) and daily needs. On May 19, 2015 there was pollution in the Batang Asam River due to the spillage of palm oil wastewater from one of the palm oil companies around the Batang Asam River. The water river becomes smelly and black and many fish die. The initial testing showed that BOD parameter = 10 mg / l), COD = 50 mg / l, TSS = 16 mg / l, N-Total = 3.86 mg / l, pH = 6.02, Oil and fat = 2.8 mg / l, where almost all parameters above the quality standard. The purpose of this study was to analyze status of water and water quality of Batang Asam River Using Pollution Index Method as a result of spilling palm oil wastewater. Water sampling of the Batang Asam River was carried out in 4 (four) locations, namely the upstream area of Lubuk Bernai Village, the Central area of Lubuk Lawas Village and Tanjung Bojo Village and the downstream of Kampung Baru Village (Pengabuan River Estuary). Parameters tested were pH, TSS, TDS, BOD, COD, DO, Fe, Oil and fat based on Government Regulation Number 82 of 2001 concerning Water Pollution Control and Water Quality Management For Class II. Analysis of water quality status using the Pollution Index method based on the Minister of Environment Regulation Number 115 of 2003 concerning Guidelines for Determining the Status of Water Quality. The results showed that the analysis of the average waterquality of Batang Asam River from four locations tested were parameters pH 6,8, TDS parameters 79,5 mg / l, TSS parameters 15,25 mg / l, DO parameters 3,025 mg / l, BOD parameters 2,935 mg/l, COD parameters 14,5 mg / l, Fe 0,645 mg / l and parameters of oil and fat 1 mg /l satisfy the class II water quality standard (PP No. 82 of 2001). The status of Batang Asam River shows that the status of water quality is mildly polluted with a pollution index value of 1,0 ≤ IP ≤ 5,0 which is 1,96.

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