scholarly journals Development of thermotolerant isolates of Beauveria bassiana (Bals.-Criv.) Vuill. with ethyl methanesulfonate

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Yanisa Wongwanich ◽  
Payorm Cobelli ◽  
Duangkamon Boonchuay ◽  
Teerada Wangsomboondee
Keyword(s):  
High Temperature ◽  
Beauveria Bassiana ◽  
Brown Planthopper ◽  
Fungal Growth ◽  
Nilaparvata Lugens ◽  
Ethyl Methanesulfonate ◽  
Wild Type ◽  
Pest Insects ◽  
Increasing Temperature

AbstractBeauveria bassianais an entomopathogenic fungus that is widely used in Thailand to control pest insects. However, the increasing temperature has influenced the insect control efficiency of the fungus. Therefore, determination of thermotolerant isolates ofB. bassianathat can grow and remain pathogenic at higher temperatures than its current optimum temperature may be a better way to control pest insects in a high temperature environment. Three isolates ofB. bassianaobtained from the Rice Department, Thailand were selected for mutagenesis using ethyl methanesulfonate (EMS) with subsequent screening at high temperatures (33 and 35°C). In addition, the recovery of fungal growth after exposure to a high temperature for a period of time (5–15 days) and then transferring to 25°C was evaluated. No isolates were found that grew at 35°C but one mutant isolate (BCNT002MT) produced larger diameter colonies and more spores than the corresponding wild type (WT) at 33°C. Growth and spore production of the BCNT002MT isolate were greater than its WT when incubated at 25°C for 14 days following exposure to 33°C for 7 days. In addition, the spore germination level (%) of BCNT002MT was significantly higher than its WT during culture at 25°C after prior exposure to 33°C for 5, 10 and 15 days. The pathogenicity against the brown planthopper,Nilaparvata lugens(Stål), of this mutant isolate was also prominent.

scholarly journals Identification of a female determinant gene for the sexual determination of a hemipteran insect, the brown planthopper

2019 ◽  
Author(s):  
Ji-Chong Zhuo ◽  
Hou-Hong Zhang ◽  
Yu-Cheng Xie ◽  
Han-Jing Li ◽  
Qing-Ling Hu ◽  
...  
Keyword(s):  
Sex Determination ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Female Development ◽  
Upstream Gene ◽  
Sexual Determination ◽  
Specific Alternative ◽  
Hemipteran Insect ◽  
Female Determinant

AbstractThe sex determination mechanism for hemipteran species remains poorly understood. During the sex determination of the brown planthopper (BPH), Nilaparvata lugens, one species of Hemiptera, the functions of doublesex (Nldsx) and NlTra-2 (NlTra-2) genes were identified in our previous studies. Here, we identify an upstream gene for Nldsx in the sex determination cascade, NlFmd, which acts as female determinant gene for N. lugens. The sex-specific transcript of NlFmd (NlFmd-F) encodes an arginine/serine-, and proline-rich protein that is essential for female development. The knockdown of NlFmd resulted in the development of pseudomales, with sex-specific alternative Nldsx processing, and maternal RNA interference (RNAi) against NlFmd generates male-only progeny. Moreover, homologous genes for NlFmd have also been identified in two rice planthopper species, the white-backed planthopper (WBPH, Sogotalla furcifera) and the small brown planthopper (SBPH, Laodelphax striatellus), and these genes appear to be involved in the sex determination cascades for these species. Our data suggest that the sex determination cascade in Delphacidae is conserved.

scholarly journals Compatibility Between Beauveria bassiana (Bals.) and Neem Extract against Brown Plant-Hopper (Nilaparvata lugens Stal.)

2019 ◽  
Vol 4 (1) ◽  
pp. 9
Author(s):  
Nova Laili Wisuda ◽  
Tri Harjaka ◽  
Indah Dwi Prasetyaningrum
Keyword(s):  
Beauveria Bassiana ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Spore Density ◽  
Neem Extract ◽  
Randomized Design ◽  
Speed Up ◽  
Nilaparvata Lugens Stål ◽  
And Control

Beauveria bassiana fungi and neem extract as botanical insecticide can be combined to get the synergizing effect to control brown planthopper (BPH). This study was conducted to determine the best combination between the spore density of B. bassiana and concentration of neem extract to control BPH effectively. The two factorial treatment consisted concentration of B. bassiana (106  Colony Forming Unit (CFU) and 107 CFU) and concentration of neem extract (5%, 10%, 15%), and control.  The treatments were assigned in Completely Randomized Design with three replications. The data were analyzed using ANOVA and  LSD at 5%. The test was conducted in vitro and bio assay of testing insects, BPH, was conducted using dipping method.  B. bassiana was suitable with neem extract with T value ranged from 78.58- 90.38. The mortality of BPH occurred on day 5 after application. The highest mortality (91.67%) occurred on the treatment with B. bassiana 107 CFU + neem extract 10%. The shortest LT50 was found at the treatment of B. bassiana 106 CFU+  neem extract 15%  (2.74 days) but not significantly different from B. bassiana 107 CFU + neem extract 15% (2.76 days). B. bassiana spores combined with neem extract are assumed to speed up the mortality of BPH by increasing the concentration.

High Temperature Modifies Resistance Performances of Rice Varieties to Brown Planthopper, Nilaparvata lugens (Stål)

Rice Science ◽  
2010 ◽  
Vol 17 (4) ◽  
pp. 334-338 ◽  
Author(s):  
Bao-ju WANG ◽  
Hong-xing XU ◽  
Xu-song ZHENG ◽  
Qiang FU ◽  
Zhong-xian LU
Keyword(s):  
High Temperature ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Varieties ◽  
Nilaparvata Lugens Stål

scholarly journals Growth of Beauveria bassiana Combined with MIPC Insecticide and Its Efficacy to Control the Brown Planthopper Nilaparvata lugens

2020 ◽  
Vol 7 (1) ◽  
pp. 9-18
Author(s):  
Aminudin Afandhi ◽  
Yayan Atma Kunjana ◽  
Fery Abdul Choliq ◽  
Amin Setyo Leksono
Keyword(s):  
Beauveria Bassiana ◽  
Rice Field ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Sublethal Concentration ◽  
Plant Diseases ◽  
Vegetative Phase ◽  
Significant Difference ◽  
Field Efficacy ◽  
Development Laboratory

The objectives of this study were to evaluate the efficacy of MIPC to control the brown planthopper Nilaparvata lugens, one of the most damaging pests of rice in the field and determine the effect of various of its doses on the growth of the fungus B. bassiana. The research was carried out in a rice field and in the Biological Agents Development Laboratory, Department of Pests and Plant Diseases, Faculty of Agriculture, Brawijaya University, Indonesia. The field efficacy test of the MIPC insecticide was conducted by applying 0.5-, 1.0-, 1.5-, and 2.0-kg/ha doses. The insecticidal compatibility test of MIPC with B. bassiana was conducted in 0.5-, 1.0-, 1.5-, and 2.0-g/L doses of MIPC. Application of MIPC insecticide at 0.5-, 1.0-, 1.5-, and 2.0-kg/ha doses had no significant difference in reducing N. lugens population in the vegetative phase (28 to 70 days after planting). The MIPC doses of 1.0, 1.5, and 2.0 g/L were highly toxic or incompatible with the growth of B. bassiana. Meanwhile, a sublethal concentration of MIPC insecticide of 0.5 g/L was non-toxic to B. bassiana. Therefore, this study recommends the use of 0.5 g/L MIPC combined with B. bassiana fungi.

Beauveria bassiana sensu lato granules for management of brown planthopper, Nilaparvata lugens in rice

BioControl ◽  
2014 ◽  
Vol 60 (2) ◽  
pp. 263-270 ◽  
Author(s):  
Se Jin Lee ◽  
Jeong Seon Yu ◽  
Yu-Shin Nai ◽  
Bruce L. Parker ◽  
Margaret Skinner ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Brown Planthopper ◽  
Nilaparvata Lugens

scholarly journals Comparison of upper sublethal and lethal temperatures in three species of rice planthoppers

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shahbaz Ali ◽  
Pei Li ◽  
Asad Ali ◽  
Maolin Hou
Keyword(s):  
Plant Material ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Laodelphax Striatellus ◽  
Sogatella Furcifera ◽  
Lethal Temperatures ◽  
Rice Planthoppers ◽  
Critical Thermal Maxima ◽  
Increasing Temperature ◽  
Occurrence Patterns

Abstract Temperature is an important environmental factor for ectotherms’ fitness and survival. The upper sublethal and lethal temperatures were compared between adults of three closely related destructive planthopper species, the small brown planthopper (Laodelphax striatellus, SBPH), the brown planthopper (Nilaparvata lugens, BPH), and the white-backed planthopper (Sogatella furcifera, WBPH) in the absence and presence of the host plant (Oryza sativa, var. Taichong1). Values of the critical thermal maxima (CTmax) were higher in SBPH than in both BPH and WBPH and higher in BPH than in WBPH, and values of the heat coma temperatures (HCT) were higher in both BPH and SBPH than in WBPH. CTmax and HCT values were higher in the presence than in the absence of plant material. Between sexes, females generally showed higher CTmax and HCT than males. The upper lethal temperatures (ULT50) measured in the absence of plant material were not significantly different among the planthopper species. The planthoppers also exhibited different behaviors in an increasing temperature regime, with fewer insects dropping-off from the plant in SBPH than in BPH and WBPH. These results indicate that SBPH and BPH are more heat tolerant than WBPH. The findings highlight the biological divergence in closely related planthopper species and the importance of performing the heat tolerance measurement in an ecologically relevant setting, which serves to predict seasonal and spatial occurrence patterns of the destructive planthopper species.

scholarly journals Overexpression of OsGID1 Enhances the Resistance of Rice to the Brown Planthopper Nilaparvata lugens

2018 ◽  
Vol 19 (9) ◽  
pp. 2744 ◽  
Author(s):  
Lin Chen ◽  
Tiantian Cao ◽  
Jin Zhang ◽  
Yonggen Lou
Keyword(s):  
Growth And Development ◽  
Receptor Gene ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Hatching Rate ◽  
Wild Type ◽  
Plant Growth And Development ◽  
Mechanical Wounding ◽  
Positive Role ◽  
Lignin Level

Gibberellins (GAs) play pivotal roles in plant growth and development, and in defenses against pathogens. Thus far, how the GA-mediated signaling pathway regulates plant defenses against herbivores remains largely unknown. In this study, we cloned the rice GA receptor gene OsGID1, whose expression was induced by damage from the brown planthopper (BPH) Niaparvata lugens, mechanical wounding, and treatment with salicylic acid (SA), but not jasmonic acid. The overexpression of OsGID1 (oe-GID1) decreased BPH-induced levels of SA, H2O2, and three SA-pathway-related WRKY transcripts, but enhanced BPH-induced levels of ethylene. Bioassays in the laboratory revealed that gravid BPH females preferred to feed and lay eggs on wild type (WT) plants than on oe-GID1 plants. Moreover, the hatching rate of BPH eggs on oe-GID1 plants was significantly lower than that on WT plants. In the field, population densities of BPH adults and nymphs were consistently and significantly lower on oe-OsGID1 plants than on WT plants. The increased resistance in oe-GID1 plants was probably due to the increased lignin level mediated by the GA pathway, and to the decrease in the expression of the three WRKY genes. Our findings illustrated that the OsGID1-mediated GA pathway plays a positive role in mediating the resistance of rice to BPH.

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