scholarly journals Growth variation and proline accumulation of Echinacea purpurea cultivated to CaCl2 salinity

2021 ◽  
Vol 905 (1) ◽  
pp. 012017
Author(s):  
J P Choirunnisa ◽  
Y Widiyastuti ◽  
B Pujiasmanto ◽  
A T Sakya ◽  
A Yunus

Abstract Purple coneflower (Echinacea purpurea (L.) Moench) is classified as medicinal plant comes from North America and not much developed in Indonesia. E. purpurea can be developed by utilizing suboptimal land such as saline land. This study aims to determine the effect of CaCl2 concentration on growth and proline accumulation of three accessions E. purpurea. This study used a Completely Randomized Design (CRD) with 2 factors and five replications. The first factor is three accessions E. purpurea (accession 1; 2; and 3). The two factor is four concentrations CaCl2 (0; 2500; 5000; and 10000 ppm). The observations are plant height, number of leaves, number of branches and proline accumulation. Data were analyzed using SPSS to test difference. The results showed that highest concentration of CaCl2 can inhibit growth and increase proline accumulation. The highest proline accumulation in accession 2 was 22.8002 µmol g−1, accession 2 as an indicator of accession tolerant to salinity.

2017 ◽  
Vol 24 (4) ◽  
pp. 239-242
Author(s):  
A. Sure ◽  
S. Khachane ◽  
A. Nimkar

An experimental trial was conducted to find out the effective potting mixture and low cost quality planting material through semi-hardwood cuttings propagation of endangered medicinal plant Commiphora wightii (Arn.) Bhandari. The experiment was carried out in CRD (Completely Randomized Design) with six treatments, three replications and 20 cuttings per treatment. Growth in cutting was measured by eleven parameters. Parameter such as sprouting percentage (88.67%), rooting percentage (83.45%), minimum days for sprouting (9.82), number of sprouts (7.17), length of shoot (15.25cm), diameter of shoot (3.950mm), number of leaves (24.83), chlorophyll content a, b and total (7.6290, 5.7083 and 13.3374 mg/100g, respectively) was significantly superior under potting mixture S+S+V (soil+sand+vermicompost). Furthermore rooting parameter such as number of roots (7.51), length of root (14.60cm), diameter of root (1.338mm) was found higher in media containing S+S+C (soil+sand+cocopeat).


2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Thomas Wagin ◽  
Oktavianus Lumban Tobing ◽  
Nur Rochman

This study was aimed to determine the effect of dosage of  cow manure and, dolomite on the growth and production of long  beans. This research was conducted in experimental field of Agroteknologi Department of Djuanda University Bogor. The study was prepared according to Completely Randomized Design (RAL), factorial. The first factor was the dose of cow manure according to recommendation consisting of 4 levels: 0R, 0.5 R, 1R, and 1.5R . The second factor is the recommended dolomite consisting of 4 levels: 0R, 0.5 R, 1R, and 1.5 R.  The results showed that the average height of the plant at age 6 MST, the number of leaves 4 MST and 6 MST, the number of flowers and the amount of fruit 6 MST, wet fruit weight of 3rd fruit harvest, dry weight of stem aged 8 MST influenced by cow manure.  While plant 4 MST, number of leaves 4,6 and 8 MST, number of branches 4, 6 and 8 MST, 6.8 MST of flowers and number of fruit 6,8 MST, weight lenghtof fruit harvest to-1, wet fruit weight of whole harvest 1st, dry weight of 3rd harvest, wet weight of stem, dry weight of stem, wet weight of root influenced by dolomite.Keywords: Long beans, plant height, the amount of fruit, fruit weight


2015 ◽  
Vol 61 (3) ◽  
pp. 13-22 ◽  
Author(s):  
Shamila Yadolahi Zadeh ◽  
Ali Akbar Ramin ◽  
Bahram Baninasab

SummaryThis study was conducted in order to determine the appropriate treatment for breaking dormancy and the effect of salinity on seed germination of purple coneflower (Echinacea purpureacv. Magnus), in two separate experiments. In the first experiment, five levels of gibberellic acid (GA3) (0, 250, 500, 1000, and 1500 mg×L−1) with four levels of cold moist stratification period of seeds at 5°C (0, 5, 10 and 15 days) were launched. A factorial experiment was conducted in a completely randomized design with four replications. The statistical analysis showed that concentration of 250 mg×L−1GA3with 10 days of cold moist chilling significantly increased the percentage of germination of normal seedlings and reduced the mean time of germination. In the second experiment, the seeds were chilled for 10 days at 5°C and half of them treated with 250 mg×L−1GA3for 24 hours. The seeds treated with GA3, and those non-treated were subjected to NaCl for salinity stress. The experiment was conducted using five salinity levels (0, 20, 40, 60 and 80 mM NaCl) in four replications in a completely randomized design. The results showed that purple coneflower is highly sensitive to salinity in the germination stage. The results also showed that by increasing salinity levels, the percentage of germination and normal seedlings significantly decreased and the mean time to germination increased, compared to the control treatment. But the seeds treated with GA3showed higher viability and better performance under salinity stress condition.


AgriPeat ◽  
2019 ◽  
Vol 19 (01) ◽  
pp. 30-36
Author(s):  
Administrator Journal

ABSTRACThis study aims to find the most potent combination of local microorganisms as a liquidbiofertilizer on marginal land, such as peatland. The study used Completely Randomized Design onpeat soil media in polybags, with 8 (eight) treatments and 4 (four) replicates, namely liquidbiofertilizer composition comprising a group of local microorganisms: 1) KHY, 2) IBT, 3) KHY +IBT, 4) KHY + IGT, 5) IBT + IGT, 6) KHY + IBT + IGT, 7) EM4 (Control +), 8) Aquadest(Controls -). The variables observed were plant height, number of leaves, number of branches,number of flowers of soybean crop. The results showed that the composition of the liquidbiofertilizer EM4 gave the best results on the parameters of plant height, the number of leaves, thenumber of branches, and the amount of flowers on soybean plants tested in peat soil media. Thecombined composition of local microorganisms KHY + IBT + IGT containing bacterial populationsof phosphate solvent, nitrogen-fixing bacteria, and cellulitic fungi, are still in the same group asEM4 in the treatment of the number of leaves and the number of plant flowers, therefore thecomposition of these potential local microorganisms is developed to become a liquid biofertilizerKeywords: Liquid Biofertilizer, Soybean Growth, Peat Land


Jurnal Solum ◽  
2020 ◽  
Vol 17 (2) ◽  
pp. 49
Author(s):  
Imra Atil Mardya ◽  
Gusmini Gusmini ◽  
Agustian Agustian

The endophytic bacteria are bacteria whose life cycle is partially or completely associated in the host plant tissue in various kinds of relationships (symbiosis) that it undergoes. The potential of endophytic bacteria as microbial resources is widely used as a biological control for plants, including as the producer of phytohormones and the agent of biocontrol and biofertilizer. This study aims to test selected endophyte bacterial isolates from chili as a biofertilizer to meet the nutrient requirements that are useful during the vegetative growth period of red chili in Ultisols. The study used a completely randomized design with 4 treatments and 3 replications, namely A = fertilizer N  recommendation; B = Azospirillum sp AK+ Fertilizer N recommendation; C = Azospirillum sp BT+ Fertilizer N recommendation; D = Azospirillum sp DN+ fertilizer N recommendation. Azospirillum sp AK and Azospirillum sp DN isolates gave better vegetative growth than those of Azospirillum sp BT isolates with average dry weight, respectively, 19.49 g and 18.93 g and with a height of 29.67 cm and 29.60cm. The superiority of the 2 isolates was also shown in the observation of the number of leaves 30.39 and 22.50 and the number of branches 6.45 and 6.28 respectively for Azospirillum sp AK and Azospirillum sp DN isolates.Key words : Endophytic bacteria, red chili, Ultisols


Pastura ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 1
Author(s):  
Simel Sowmen ◽  
Ifradi Ifradi ◽  
Arif Rachmat ◽  
Tri Silfani ◽  
Karyadinata Karyadinata

This research was aims to assess effect of the different phospor fertilizer on the growth and nodules in Pueraria javanica inoculated with Arbuscular Fungi Micorrhyza. This experimental design was Completely Randomized Design (CRD) with three phospor fertilizer treatments: P0 = 0 kg/ha phosphor (0, g/pot), P1 = SP36 200 kg/ha (1 g/pot), and P2 = rock phosphate 200 kg/ha (1 g/pot), with four replicates. The whole pot treatments received mycorrhizal inoculation. The variables measured were plant height, number of leaves, number of branches, root length, number of root nodule, number of active nodules , and the percentage of active nodules. The results of data analysis show that the treatment has no effect (P > 0.05) for all parameters. The results showed that the presence of mycorrhizal inoculation, macro and micronutrient elements of Pueraria javanica already fulfilled without phosporus fertilizing in ultisol. Key words: AMF, phosphor, Root nodule, rock phosphate, Pueraria javanica


Pastura ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 86
Author(s):  
Witariadi N. M. ◽  
N. N. C. Kusumawati

This experiment to determine the effect of substitution of urea fertilizer with bio slurry fertilizer on productivity of Panicum maximum cv. Trichoglume grass. The experiment was conducted in a greenhouse for 3 months. The design was used completely randomized design (CRD), with seven treatments and each treatment repeated five times. The treatment was dosage of 200 urea kg/ha (A); 150 kg urea/ha + 10 tons cow bio slurry /ha (B); 100 kg urea/ha + 20 tons cow bio slurry/ha (C); 50 kg urea/ha + 30 tons cow bio slurry/ha (D); 150 kg urea/ha + 10 tons pig bio slurry/ha (E); 100 kg urea/ha + 20 tons pig bio slurry/ha (F); 50 kg urea/ha + 30 tons pig bio slurry/ha (G). Variables observed were plant height, number of tillers, number of branches, number of leaves, leaf dry weight, stem dry weight, total forage dry weight, leaf area per pot, and leaf dry weight ratio with stem dry weight. The results showed that the effectiveness of urea fertilizer substitution with pig bio slurry gave the best growth and production of grass Panicum maximum cv. Trichoglume, where treatment (G) with substitution of 50 kg urea/ha + 30 tons pig bio slurry/ha gave the highest yield. It can be concluded increasng of growth and production of grass Panicum maximum cv. Trichoglume can be substituted with 50 kg urea/ha + 30 tons pig bio slurry/ha. Keywords: substitution, urea, bio slurry, productivity, Panicum maximum


2020 ◽  
Vol 16 (1) ◽  
pp. 49-62
Author(s):  
محي الدين جمعة عبد الله

The effect of adding different sugar concentrations of five treatments (T1, T2, T3, T4, T5) on rose growth CV Sarah in vitro was carried out in the Sudan University Laboratories. Data were collected for plant height, number of branches, number of leaves, and fresh weight. Statistical analysis was conducted using a completely randomized design, to find the least significant difference to compare between treatment means for MS (Murashige and Skoog) medium. Data analysis showed that the application of sugar at concentrations of 15, 30, and 45gms gave the highest means for the different growth parameters and fresh weight at a significant level, whereas the addition of sugar at 60gms concentration and sugar-free gave no significant increases. This showed clearly that addition of sugar was essential for plant growth, greater concentrations gaviz 60gm negative results, Addition of sugar at the above-mentioned concentration from 15mg,45gm to parameters studied the growth media in vitro gave a positive increase in rose growth.


2018 ◽  
Vol 3 (2) ◽  
Author(s):  
Lukman Fahmi ◽  
Arifah Rahayu ◽  
Yanyan Mulyaningsih

Fertilizer administration is a way to increase the productivity of edamame plants.  Synthetic fertilizer is commonly used in edamame cultivation today.  However, excessive use of synthetic fertilizer could reduce soil fertility so that substitution of it with biofertilizer is needed.  This study was aimed at assessing the effects of biofertilizer and synthetic fertilizer administration on the growth and production of edamame plants.  A completely randomized design in a factorial pattern was used.  The first factor was biofertilizer rates consisting of three levels namely no fertilizer (0% R), 5 ml/l (50% R), and 10 ml/l (100% R).  synthetic fertilizer was given in five levels consisting of no fertilizer (0% R), 25% recommended rate (urea 37.5 kg/ha, SP-36 37.5 kg/ha, and KCl 25 kg/ha), 50% recommended rate (urea 75 kg/ha, SP-36 75 kg/ha, and KCl 50 kg/ha), 75% recommended rate (urea 112.5 kg/ha, SP-36 112.5 kg/ha, and KCl 75 kg/ha), and 100% recommended rate (urea 150 kg/ha, SP-36 150 kg/ha, and KCl 100 kg/ha).Results showed that plants given biofertilizer by 100% recommended rate had significantly higher height, number of leaves, and fresh and dry root weight in 5 weeks after planting (WAP).  Synthetic fertilizer administration by 100% recommended rate gave significantly higher plant height, number of branches, number of leaves, and fresh and dry root weight in 5 weeks after planting (WAP).  Interaction of 100% recommended rate of biofertilizer and 100% recommended rate of synthetic fertilizer resulted in significantly higher number of leaves and number of flowers. Keywords: edamame plant. Biofertilizer, synthetic fertilizer


Agrologia ◽  
2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Mia Munggarani ◽  
Erni Suminar ◽  
Anne Nuraini ◽  
Syariful Mubarok

Mericlone shoots are shoots of propagation using meristem explants which aim to produce seeds in a short time, large number, and disease-free seed. The aim of this experiment was to find out the best types and concentration of cytokinin to mericlone shoot multiplication of potato Jala Ipam variety. The experiment was carried out at Seed Technology Tissue Culture Laboratory of Agriculture, Padjadjaran University during November 2016 until February 2017. The experimental design was Completely Randomized Design with 13 treatments and 4 replications. Murashige and Skoog Medium with different concentration and types of cytokinine was used in this experiment. The result showed that the type and concentrations of cytokinin give the different effect on the mericlone shoot multiplication of potato Jala Ipam variety. MS medium with the additional of cytokinin 2 -iP 1.5 mg L-1 significantly increased the mericlone shoot multiplication of potato Jala Ipam variety at the variable number of leaves, number of nodes, and the number of branches.


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