scholarly journals Effect of gibberellic acid, stratification and salinity on seed germination of Echinacea purpurea cv. Magnus

2015 ◽  
Vol 61 (3) ◽  
pp. 13-22 ◽  
Author(s):  
Shamila Yadolahi Zadeh ◽  
Ali Akbar Ramin ◽  
Bahram Baninasab
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Salinity Stress ◽  
Echinacea Purpurea ◽  
Purple Coneflower ◽  
Randomized Design ◽  
Salinity Levels ◽  
The Mean ◽  
Completely Randomized Design ◽  
Mean Time

SummaryThis study was conducted in order to determine the appropriate treatment for breaking dormancy and the effect of salinity on seed germination of purple coneflower (Echinacea purpureacv. Magnus), in two separate experiments. In the first experiment, five levels of gibberellic acid (GA3) (0, 250, 500, 1000, and 1500 mg×L−1) with four levels of cold moist stratification period of seeds at 5°C (0, 5, 10 and 15 days) were launched. A factorial experiment was conducted in a completely randomized design with four replications. The statistical analysis showed that concentration of 250 mg×L−1GA3with 10 days of cold moist chilling significantly increased the percentage of germination of normal seedlings and reduced the mean time of germination. In the second experiment, the seeds were chilled for 10 days at 5°C and half of them treated with 250 mg×L−1GA3for 24 hours. The seeds treated with GA3, and those non-treated were subjected to NaCl for salinity stress. The experiment was conducted using five salinity levels (0, 20, 40, 60 and 80 mM NaCl) in four replications in a completely randomized design. The results showed that purple coneflower is highly sensitive to salinity in the germination stage. The results also showed that by increasing salinity levels, the percentage of germination and normal seedlings significantly decreased and the mean time to germination increased, compared to the control treatment. But the seeds treated with GA3showed higher viability and better performance under salinity stress condition.

scholarly journals Pengaruh Konsentrasi dan Lama Perendaman GA3 terhadap Perkecambahan Benih dan Pertumbuhan Bibit Johar (Cassia seamea)

2021 ◽  
Vol 5 (1) ◽  
pp. 1-6
Author(s):  
Ahmad Yunus ◽  
Arifiya Qifni ◽  
Puji Harsono ◽  
Bambang Pujiasmanto
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Gibberellic Acid ◽  
Active Compound ◽  
Root Length ◽  
Research Method ◽  
Percentage Germination ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Germination And Growth

Johar (<em>Cassia seamea</em>) is a medicinal plant with a cassiarin A compound to combat malaria and has a low Cassiarin A is an active compound in Johar (<em>Cassia seamea</em>) medicinal plant capable of combating malaria, despite showing a low seed production rate due to dormancy. This inactivity is severed using gibberellic acid (GA<sub>3</sub>) treatments. This study aims to determine the effects of concentration and soaking period of GA<sub>3</sub> on seed germination and growth of Johar seedlings. A completely randomized design (CRD), consisting of 2 treatments was applied as the research method. The first treatment employed GA<sub>3</sub> concentrations of 0, 45, 90 and 135 ppm, while the second was based on the soaking interval at 6, 12, 18 and 24 hours. The results showed the capacity of GA<sub>3</sub> able to shatter the seed dormancy with percentage germination of 43.03% and a concentration of 50.28 ppm. Therefore, the interaction of the two treatments significantly influenced the growth of Johar seedlings. Consequently, the concentration of GA<sub>3</sub> at 79.89 ppm increased the root length to 52.19 cm, while 84.68 ppm produced a total of 20.91 roots, after a 6 hour soaking. In addition, the 96.67 ppm expanded the stem diameter by 0.93 cm.

scholarly journals Bioregulators on seed germination and seedling growth of sweet basil

2020 ◽  
Vol 11 ◽  
pp. e3324
Author(s):  
Larissa Santos Castro ◽  
Danúbia Aparecida Costa Nobre ◽  
Daniel Andrés Villegas Hurtado ◽  
Willian Rodrigues Macedo
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Germination Rate ◽  
Ocimum Basilicum ◽  
Shoot Length ◽  
Naphthalene Acetic Acid ◽  
Initial Growth ◽  
Randomized Design ◽  
Root And Shoot Length ◽  
Completely Randomized Design

Basil (Ocimum basilicum L.) plants have multiple uses, ranging from spice to cosmetic purpose, besides being a source of essential oil and aromatic. These plants were commonly propagated by seed, however, few agrotechnologies are used to enhance the seed germination and initial growth in this crop. For this reason, our research aimed to evaluate aspects of seed germination of Ocimum basilicum L. cv. Limoncino, subjected to different bioregulators applied via substrate imbibition. The experiment was carried out in completely randomized design, with four treatments, as follows: T1: control (distilled water), T2: T13 a solution of a product formulated based on indolbutyric acid + naphthalene acetic acid + gibberellic acid (0.2 mL L-1, Liko Química), T3: gibberellic acid (0.5 mM L-1, progibb-400®) and T4: brassinosteroids (0.1 mM L-1). We evaluated: germination rate, germination speed index (GSI), first count, root and shoot length and chlorophyll a, b, total and carotenoids. The bioregulators did not influence germination, first count, GSI and photosynthetic leaf pigment, but application of gibberellic acid and brassinosteroids improve the shoot and root length, respectively. The gibberellic acid and brassinosteroids are promising bioregulators for stimulating initial basil growth.

scholarly journals Integrated effects of bacteria and fungi biofertilizers on morphological traits, antioxidants indices, and polyphenol compounds of quinoa (Chenopodium quinoa Willd.) under salinity condition

2020 ◽  
Author(s):  
Giti Karimi ◽  
Latifeh Pourakbar ◽  
Sina Siavash Moghaddam ◽  
Jelena Popović-Djordjević
Keyword(s):  
Salinity Stress ◽  
Morphological Traits ◽  
Chenopodium Quinoa ◽  
Dry Weight ◽  
Cinnamic Acids ◽  
Polyphenol Compounds ◽  
Randomized Design ◽  
Salinity Levels ◽  
Completely Randomized Design ◽  
Bacteria And Fungi

Abstract It is imperative to assess the potential of halophyte plant species, such as quinoa, in resisting high salinity levels in arid and semi-arid regions where the productivity of crops is dramatically affected. A factorial experiment based on a completely randomized design with three replications was conducted to explore the effect of integrated biofertilizer, on morphological traits, antioxidants, and polyphenol compounds of quinoa under salinity stress. The studied factors included NaCl salinity stress at three levels of 0, 150, and 300 mM (S0, S150, and S300, respectively), Trichoderma (T) fungus at two levels (its use and non-use), and biofertilizer at three levels (control, nitrogen (FN), and phosphorus (FP). The means of the studied traits showed that the highest shoot length and dry weight was related to S0T0FN, the highest root length to S150TFN, the highest root dry weight to S0T0FN, and the highest phenol and flavonoid contents to S300TFP and S0TFN treatments. Among polyphenols, the highest caffeic acid, rutin, coumaric acid, and quercetin were observed in S0TFP, and the highest levels of chlorogenic, rosmarinic, cinnamic acids, and apigenin (mg/kg) were observed in S0TFN. To sum up, Bacteria and fungi biofertilizers were effective on the studied traits at the three salinity levels.

scholarly journals Plausible Mechanisms by Which Ultrasonic Waves Affect Seeds

2016 ◽  
Vol 74 (1) ◽  
pp. 85-92
Author(s):  
Mohammad Eteghadi Pour ◽  
Mahsa Hobbi ◽  
Hadi Ghasemi ◽  
Meisam Nazari
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Germination Percentage ◽  
Ultrasonic Waves ◽  
Cell Area ◽  
Water Imbibition ◽  
Randomized Design ◽  
Cell Wall Rigidity ◽  
The Mean ◽  
Completely Randomized Design

Abstract To study the effect of sonication on the seed germination percentage and rate as well as the cell area of barley (Hordeum vulgare L.) a laboratorial experiment was performed as Completely Randomized Design (CRD) with 3 replications. The results indicated that the ultrasonic waves affect the seed germination, germination rate and cell area significantly (at 0.05). The results of the mean comparison tests (LSD, 0.05) showed that the highest germination percentage (100 %), germination rate and cell area (1370.71 μ2) is achieved through 15 minutes exposure to ultrasonic waves. It is concluded that weakening the seed’s cell wall rigidity by sonication results in more and faster water imbibition by the cells and improved germination.

scholarly journals Germination and survival of Aechmea gamosepala on different substrates

2019 ◽  
Vol 25 (3) ◽  
pp. 225-230 ◽  
Author(s):  
Adilson Anacleto ◽  
Luís Fernando Roveda ◽  
Rafaela Aparecida Santoro Ramos
Keyword(s):  
Agricultural Land ◽  
Seedling Survival ◽  
Ornamental Plants ◽  
Sprinkler Irrigation ◽  
Easy Access ◽  
Experimental Unit ◽  
Randomized Design ◽  
The Mean ◽  
Completely Randomized Design ◽  
Mean Time

Abstract The State of Parana is one of the Brazilian regions with the highest incidence of extraction of bromeliads, among the factors for the occurrence of this situation, they highlight a great demand for this species as ornamental flowers and the easy access to forests, few cultivation ponds and difficulties for farmers in cultivation processes, especially in the early stages of cultivation. The species Aechmea gamosepala Wittmack stands out among the species of this group of ornamental plants (Bromeliaceae) with strong commercial appeal and consequently of extraction. Given this context, a study was carried out to evaluate the germination and survival of seeds of the species. Five alternative types of substrates easily accessible to farmers were tested: coconut husk fiber, Plantmax®, earthworm humus, humidified Pinnus husk and sieved agricultural land. The experiment was conducted in a completely randomized design with 4 replications and 25 seeds per experimental unit at room temperature in a greenhouse, with sprinkler irrigation for a period of 90 days. The mean time for the onset of germination was 6.95 days, averaging 74.8% at the peak of germination, but there were no significant statistical differences between the treatments. For the seedling survival, humidified Pinnus husk substrates (70.8%) and coconut fiber house (80.4%) proved to be the best substrates for Aechmea gamosepala Wittmack.

Identification of the Effect of Sulfur Spray on Okra (AbelmoschusesculentusL. var. MI 5)Seedlings

2016 ◽  
Vol 3 (4) ◽  
Author(s):  
NILANTHI DAHANAYAKE ◽  
DDPBD DEHIGASPITIYA ◽  
AG MAYURI DHANANJANIE ◽  
AGKMWS ATAPATTU ◽  
PCD PERERA
Keyword(s):  
Growth Parameters ◽  
Disease Incidence ◽  
Foliar Spray ◽  
Leaf Length ◽  
Randomized Design ◽  
Number Of Leaves ◽  
The Mean ◽  
Completely Randomized Design ◽  
Mean Time ◽  
Range Test

The study investigated that the effect of sulfur solutions as foliar spray with different concentrations on some selected growth parameters of ladies fingers plants. Pot experiment was conducted with six treatments (2g/L, 4g/L, 6g/L, 8g/L and 10g/L sulfur solutions) and three replicates. For each pot,two plants were maintained throughout the experiment. After seedling emerging, sulfur solutions were sprayed in every week using OSATU sprayer (5 L), until all the leaves got wet. After 30 days physiological features (Number of leaves, leaf length andleaf width) were recorded. Pest and disease incident also recorded at the mean time. Completely Randomized Design (CRD) with five replicates was used. Statistical analysis was performed with Duncan’s multiple range test using SAS software (version 9.1.3). Result showed that the highest significant values in number of leaves (26), leaf length (21cm) and leaf width (24cm) were observed that in 10g/L solution of sulfur applied as foliar spray. With the increasing concentration of sulfur solutions the values for the selected physiological features also increased. Pest and disease incidence was significantly higher in control experiment while all the other treated plants don’t showed any physiological damage or symptom. The results revealed that the application of sulfur as a foliar spray can be effectively use in increase the net photosynthetic area and to reduce the pest and disease incidence.

scholarly journals Response on Growth and Production of Vetiver (Vetiveria zizanioides L.) on Gibberelin Under Salinity Stress Conditions

2021 ◽  
Vol 4 (1) ◽  
pp. 13-20
Author(s):  
Aisar Novita ◽  
Nini Rahmawati ◽  
Fitra Syawal Harahap ◽  
Hilwa Walida ◽  
Abdul Rahman Cemda ◽  
...  
Keyword(s):  
Salinity Stress ◽  
High Salinity ◽  
Stress Conditions ◽  
Vetiveria Zizanioides ◽  
Green House ◽  
Randomized Design ◽  
Salinity Levels ◽  
Completely Randomized Design

Vetiver (Vetiveria zizanioides L.) plants are able to protect the soil from erosion and are tolerant of salinity stress. However, at high salinity levels, vetiver plants show a decreasing growth. The application of gibberelin is expected to increase the growth of vetiver in salinity stress conditions. The purpose of this study was to evaluate the growth responsiveness and production of vetiver the application of gibberelin under salinity stress conditions. This research was conducted in the green house of the Faculty of Agiculture, Universitas Sumatera Utara, Medan. This study used a non-factorial completely randomized design, namely the salinity stress (S) which consisted of 3 levels, each treatment was repeated 3 times, so that 9 treatment combinations were obtained: the concentration of gibberelin was 0 (no treatment), 50 ppm and 100 ppm.

scholarly journals 301 Gibberellic Acid (GA3) and Light Affect Germination of Echinacea angustifolia Seeds

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 443D-443
Author(s):  
Keun Ho Cho ◽  
Chiwon W. Lee ◽  
Kyu-Min Lee
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Bacterial Infections ◽  
Market Demand ◽  
Echinacea Angustifolia ◽  
Purple Coneflower ◽  
Percent Germination ◽  
Quadratic Response ◽  
Mean Time ◽  
Related Compounds

The narrow-leaved purple coneflower (Echinacea angustifolia) produces echinacin and related compounds in the root, which are known to have immune and curative properties against viral, fungal, and bacterial infections. In recent years, cultivation of this species has increased in response to growing market demand for natural medicinal remedies. The objective of this study was to determine the influence of gibberellic acid and light on the germination of E. angustifolia seeds. Seeds soaked for 24 h in 0, 1, 5, 10, 50, 100, 250, 500 or 1000 mg/L GA3 solution were germinated on Whatman #1 filter paper inside petri dishes at 22 °C with or without light (80 μmol·m-2·s-1) for 21 days. The seeds germinated poorly in dark with the final percent germination range from 10% (GA3 1000 mg/L) to 36% (GA3 250 mg/L). Under light, seed germination showed a quadratic response (r = 0.84) to GA3 concentration. Percent germination exceeded 90% at 10, 50, and 100 mg/L GA3 with the mean time (T50) to germinate varying at 10.5, 11.7, and 13.3 days, respectively, under light. Seed germination under light was <10% when treated with 500 and 1000 mg/L GA3. In general, seed germination was best when treated with 10 or 50 mg/L GA3 under light. Results of this research may well be used in enhancing seed germination during field establishment of E. angustifolia.

scholarly journals Growth variation and proline accumulation of Echinacea purpurea cultivated to CaCl2 salinity

2021 ◽  
Vol 905 (1) ◽  
pp. 012017
Author(s):  
J P Choirunnisa ◽  
Y Widiyastuti ◽  
B Pujiasmanto ◽  
A T Sakya ◽  
A Yunus
Keyword(s):  
Medicinal Plant ◽  
Echinacea Purpurea ◽  
Proline Accumulation ◽  
Purple Coneflower ◽  
Randomized Design ◽  
Growth Variation ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Saline Land ◽  
Number Of Branches

Abstract Purple coneflower (Echinacea purpurea (L.) Moench) is classified as medicinal plant comes from North America and not much developed in Indonesia. E. purpurea can be developed by utilizing suboptimal land such as saline land. This study aims to determine the effect of CaCl2 concentration on growth and proline accumulation of three accessions E. purpurea. This study used a Completely Randomized Design (CRD) with 2 factors and five replications. The first factor is three accessions E. purpurea (accession 1; 2; and 3). The two factor is four concentrations CaCl2 (0; 2500; 5000; and 10000 ppm). The observations are plant height, number of leaves, number of branches and proline accumulation. Data were analyzed using SPSS to test difference. The results showed that highest concentration of CaCl2 can inhibit growth and increase proline accumulation. The highest proline accumulation in accession 2 was 22.8002 µmol g−1, accession 2 as an indicator of accession tolerant to salinity.

scholarly journals Genetic control of thermoinhibition tolerance in lettuce seeds

2021 ◽  
Vol 56 ◽  
Author(s):  
Daniele Fátima de Oliveira ◽  
Pedro Yuri Cavasin ◽  
Sylmara Silva ◽  
Natália Souza Oliveira ◽  
Cleiton Lourenço de Oliveira ◽  
...  
Keyword(s):  
Seed Germination ◽  
Genetic Control ◽  
Additive Effects ◽  
Narrow Sense ◽  
Narrow Sense Heritability ◽  
F2 Population ◽  
Lettuce Seeds ◽  
Randomized Design ◽  
The Mean ◽  
Completely Randomized Design

Abstract The objective of this work was to assess the genetic control of tolerance to thermoinhibition in lettuce (Lactuca sativa) seeds. Seeds of the F1, F2, and F2:3 generations derived from the cross between cultivars Everglades (tolerant to thermoinhibition) and Verônica (sensitive to thermoinhibition) were used, besides seeds from the parents. Seed germination tests were conducted in a completely randomized design, with four replicates of each parent, eight of F1, and four of each of the 26 F2:3 progenies. The mean of the F2 population was considered as the mean of the sample of the 26 progenies. The genetic control of thermoinhibition tolerance in lettuce seeds is attributed to one or a few genes. The additive effects are more expressive than the nonadditive ones, and narrow-sense heritability is relatively high, allowing the prediction of success in selection.

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