scholarly journals Image Segmentation for Reflected-Light Microscopy: Some Theoretical Approaches

2021 ◽  
Vol 906 (1) ◽  
pp. 012121
Author(s):  
Stefano Pagnotta ◽  
Andrea Aquino ◽  
Marco Lezzerini

Abstract Often when we approach the study of lithologies coming from the urban environment, but in general, even from any other environment, be it a quarry, a mine, an outcrop of our interest, the first study we carry out is the one in reflected-light optical-microscopy. Reflected-light microscopy in respect to transmitted-light microscopy requires minimal sample preparation, having to polish a single surface and without the need to thin the samples to allow light to pass through them. It may be useful, already in the first analysis, to try to produce quantitative data on what we are observing. A further advantage of reflected light in an urban environment is that of being able to observe and describe the formation or interaction between opaque minerals and the environment. Information that we lose by passing directly to the transmitted light. The information that can be useful to us and that we can obtain are the relative porosity of the sample, the texture (when easily recognizable in reflected light), the maximum size and shape of the edges of the grains. To all this is added the relationship between the areas of the different crystallites identified and the possible background mass, which cannot be solved on the observation scale. When we are dealing with many samples, we do not always have the time to be able to study individually sample by sample through images, so we resort to the use of image analysis tools for image segmentation and analysis. Among these, the main thresholding method with the Otsu method, the segmentation with the k averages algorithm, and, finally, a neural network of the SOM type. In this short work, we will review the main methods of image segmentation plus an innovative method developed by our group, highlighting its strengths and weaknesses.

Author(s):  
Alan Boyde ◽  
Milan Hadravský ◽  
Mojmír Petran ◽  
Timothy F. Watson ◽  
Sheila J. Jones ◽  
...  

The principles of tandem scanning reflected light microscopy and the design of recent instruments are fully described elsewhere and here only briefly. The illuminating light is intercepted by a rotating aperture disc which lies in the intermediate focal plane of a standard LM objective. This device provides an array of separate scanning beams which light up corresponding patches in the plane of focus more intensely than out of focus layers. Reflected light from these patches is imaged on to a matching array of apertures on the opposite side of the same aperture disc and which are scanning in the focal plane of the eyepiece. An arrangement of mirrors converts the central symmetry of the disc into congruency, so that the array of apertures which chop the illuminating beam is identical with the array on the observation side. Thus both illumination and “detection” are scanned in tandem, giving rise to the name Tandem Scanning Microscope (TSM). The apertures are arranged on Archimedean spirals: each opposed pair scans a single line in the image.


Author(s):  
Fanie du Toit

Reconciliation emphasizes relationships as a crucial ingredient of political transition; this book argues for the importance of such a relational focus in crafting sustainable political transitions. Section I focuses on South Africa’s transition to democracy—how Mandela and De Klerk persuaded skeptical constituencies to commit to political reconciliation, how this proposal gained momentum, and how well the transition resulted in the goal of an inclusive and fair society. In developing a coherent theory of reconciliation to address questions such as these, I explain political reconciliation from three angles and thereby build a concept of reconciliation that corresponds largely with the South African experience. In Section II, these questions lead the discussion beyond South Africa into some of the prominent theoretical approaches to reconciliation in recent times. I develop typologies for three different reconciliation theories: forgiveness, agonism, and social restoration. I conclude in Section III that relationships created through political reconciliation, between leaders as well as between ordinary citizens, are illuminated when understood as an expression of a comprehensive “interdependence” that precedes any formal peace processes between enemies. I argue that linking reconciliation with the acknowledgment of interdependence emphasizes that there is no real alternative to reconciliation if the motivation is the long-term well-being of one’s own community. Without ensuring the conditions in which an enemy can flourish, one’s own community is unlikely to prosper sustainably. This theoretical approach locates the deepest motivation for reconciliation in choosing mutual well-being above the one-sided fight for exclusive survival at the other’s cost.


2016 ◽  
Vol 40 (2) ◽  
pp. 81-105
Author(s):  
Gabriela Cruz

Sr. José do capote, a worker and an opera lover, is the monad contemplated in this article. He is a theatrical figure, the protagonist of the one-act burlesque parody Sr. José do capote assistindo a uma representação do torrador (Sr. José of the Cloak attends a performance of The Roaster, 1855), but also an idea that expresses in abbreviated form the urban environment of nineteenth-century Lisbon, the theatrical and operatic sensibility of its citizens, and the politics of their engagement with the stage. This article is a history of Il trovatore and of bel canto claimed for a nascent culture of democracy in nineteenth-century Portugal.


1994 ◽  
Vol 112 (3) ◽  
pp. 252-258 ◽  
Author(s):  
Marc Schmutz ◽  
Jacques Lang ◽  
Sabine Graff ◽  
Alain Brisson

The Analyst ◽  
2014 ◽  
Vol 139 (20) ◽  
pp. 5247-5253 ◽  
Author(s):  
Sergiy Patskovsky ◽  
Eric Bergeron ◽  
David Rioux ◽  
Mikaël Simard ◽  
Michel Meunier

We report a hyperspectral reflected light microscopy system for plasmonic nanoparticle (NP) imaging, and compare with a conventional darkfield method for spatial localization and spectroscopic identification of single Au, Ag and Au/Ag alloy NPs incubated with fixed human cancer cell preparations.


2013 ◽  
Vol 734-737 ◽  
pp. 2912-2916
Author(s):  
Hui Li ◽  
Ping He

Automation strain measurement of the sheet metal deforming becomes one of the important application fields of computer vision. The algorithm of image segmentation based on adaptability threshold was presented for image segmentation of metal steel. In order to validate the proposed method, it is tested and compared with Ostu method and the one-dimensional maximum entropy method. Experiment results indicate that the method is simple and effective, and has an advantage of reservation of the main features of the original image.


1961 ◽  
Vol 4 (1) ◽  
pp. 13-21
Author(s):  
R. G. de Buda

On an orientable surface of genus p, a set of 2p fundamental circuits can be selected which all pass through a single point A. After cutting along the 2p circuits, the surface can be unfolded into a flat region bounded by a 4p-gon so that: the set of vertices corresponds to the one point A; and the 2p pairs of edges to the 2p fundamental circuits; and the interior of the polygon to the remainder of the surface. If the edges of the polygon are directed, the 2 edges which correspond to one fundamental circuit will be directed in opposite sense, since the surface is orientable [1]. The sequence and direction of the edges is the same as the sequence of the fundamental circuits.


2021 ◽  
Vol 12 (8) ◽  
pp. 2161-2179
Author(s):  
Daiane Maria de Genaro Chiroli ◽  
Raíza Conde Coradazi ◽  
Fabio Jose Ceron Branco ◽  
Yslene Rocha Kachba ◽  
Franciely Velozo Aragão ◽  
...  

Healthcare logistics play an important role in management, being attributed the activities of acquisition, distribution and movement of materials, professionals and patients. This work aims to develop a study, using the healthcare logistics in the movement of patients in the third health region of Paraná, proposing a linear programming problem that will pass through a computational simulation, considering the existing demands and constraints in the system, aiming to optimize the flow of patients from this region. The present study developed four mathematical models, based on demands and constraints followed by linear programming in order to find the best possible solution for the flow of patients from the third health region of the state of Paraná. The study developed reached its goal of optimization, generating an economy in the transportation of patients. Through the analysis of the results, it is concluded that the model that best suits the presented problem is the one of costs minimization, since the one of vehicles presented higher costs. Possibly the model that minimizes the vehicles would bring better results if the vehicles were not outsourced, but of the Ponta Grossa City Hall (PMPG). Was possible to verify the importance of the theme, especially when referring to the flow of patients in the health services due to the lack of studies with this specific approach. Even with the scarcity of data, it is possible to notice the potential for improvements on this patient transport system.


2016 ◽  
Vol 11 (17) ◽  
Author(s):  
Miguel Ángel Vázquez Ruiz

Resumen:El proceso de globalización de los procesos económicos, a primera vista sugiere un mundo de dimensiones homogéneas, muy interrelacionado entre sí y con igualdad de oportunidades de desarrollo para todos los países. Pero la realidad se desenvuelve en otra lógica: la globalización impulsa dinámicas muy segmentadas, donde el mundo vive las paradojas de la conformación de bloques regionales entre países y de regiones diferenciadas al interior de estos. En este sentido, uno de los espacios donde en la actualidad, por una razón u otra, se dan relaciones peculiares, son las fronterizas. Hay países donde los vínculos fronterizos se expresan como conflictos étnicos y religiosos; en cambio en otros, las conexiones más importantes son de índole económica y demográfica. Este es el caso de la frontera entre México y Estados Unidos, espacio donde se reproduce una de las relaciones binacionales más intensas entre países. En el presente artículo, se pretende avanzar en hacer una relectura de la frontera norte de México y sur de Estados Unidos, considerándola una región integrada por dos subregiones: la estadounidense y la mexicana. Para ello, se pasa revista a los más importantes enfoques teóricos para entender esa realidad, y se propone su revisión a la luz de las constantes modificaciones en ésta, que conducen a agotamientos muy rápidos en los "paradigmas" de análisis que cada autor del tema utiliza. Este planteamiento se documenta con la aportación de elementos cuantitativos y cualitativos acerca de las partes que configuran la región y, particularmente, se destacan las distintas modalidades de corredores económicos como medios de vinculación entre las "subregiones". Se plantea también reflexionar acerca de aspectos poco estudiados en estas últimas, como sería el perfil de los actores empresariales, básico para entender sus niveles de competitividad en la globalización a partir de una plataforma regional.Palabras clave: Globalización, Zonas fronterizas, Frontera México-Estados Unidos, Corredores económicos, Economía fronteriza.Abstract:The globalization of economic processes, at first sight, suggests a very inter- related world of homogenous dimensions, with equal opportunities of development for all countries. But reality comes about with another logic: globalization furthers very segmented dynamics, where the world experiences the paradox of the establishment of regional blocks among countries and regions that are differentiated within such blocks. In this sense, border areas are one of the spaces where presently, due to one reason or another, peculiar relations occur. There are countries where border ties are expressed as ethnic and religious conflicts, whereas, there are others, in which the most important connections are of an economic and demographic nature. This is the case of the Mexico-US border, space where one of the most intense binational relations between countries takes place. This article intends to review Mexico?s northern border and the United States southern border, considering it a region integrated by two subregions: the one of the United States and the one of Mexico. For such purpose, the most important theoretical approaches is reviewed in order to understand said reality. Its review is proposed in view of its constant modifications that lead to very fast depletions in the "paradigms" of analysis used by each author who writes about the subject. This statement is documented with the contribution of qualitative and quantitative elements about the parts that form the region, particularly underscoring the different modalities of economic corridors as means to link the "subregions". Statements are also made that lead to reflect on aspects that have been little studied in the latter, such as the pro file of the business actors, that is basic to understand their levels of competitiveness in globalization as of a regional platform .Key words: Globalization, Borderlines, USA-Mexico borderline, Economic corridors, Borderline economics.


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