scholarly journals Growth response of diploid and tetraploid taro (Colocasia esculenta (L.) Schott) shoot culture to drought stress using polyethylene glycol

2021 ◽  
Vol 913 (1) ◽  
pp. 012016
Author(s):  
A Wulansari ◽  
A Purwito ◽  
D Sukma ◽  
TM Ermayanti
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Growth Response ◽  
Shoot Culture ◽  
Proline Content ◽  
Plant Tolerance ◽  
Two Factors ◽  
Number Of Leaves ◽  
Mortality Percentage

Abstract Taro genetic improvement through polyploidy induction is expected to be tolerant to abiotic stress. Several studies have shown that polyploidy plants have a higher adaptability to dry environments. In vitro selection technique for evaluation of plant tolerance to drought stress can be done by applying polyethylene glycol (PEG) as a selection agent. The aim of the research was to investigate the growth response of diploid and tetraploid taro shoot culture to drought stress using PEG. The experiment was conducted using a completely randomized design with two factors. The first factor was the concentration of PEG at 0, 5, 10, 15 and 20%. The second factor was Bentul taro clones which were 1 diploid clone and 2 tetraploid clones (clones 4.6.3 and 5.4.4). PEG was added to liquid MS medium containing 2 mg/l BAP. Observations of growth variables were carried out every week until the six weeks of culture. Fresh and dry weights, mortality percentage and proline content were determined at six weeks of culture. The results showed that the addition of PEG in liquid medium significantly affected the number of leaves, petiole length, number of roots, fresh and dry weights, as well as shoot mortality percentage. Differences in clones significantly affected the number of leaves, fresh and dry weights. The two factors tested (PEG and clones), gave an interaction on the number of leaves, fresh and dry weights. The proline content in all diploid and tetraploid clones showed an increase with increasing PEG concentration. The LC50 value in diploid clones was 9.82%, in tetraploid clones 4.6.3 and 5.4.4 were 14.14 and 15.45%, respectively. The results showed that PEG at 10% and 15% could be used for in vitro selection to drought stress both for diploid and tetraploid taro.

scholarly journals PLANT GROWTH OF EGGPLANT (Solanum melongena L.) IN VITRO IN DROUGHT STRESS POLYETHYLENE GLYCOL (PEG)

2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Erna Siaga ◽  
Awang Maharijaya ◽  
Megayani Sri Rahayu
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Plant Growth ◽  
Solanum Melongena ◽  
Dry Land ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves

Drought stress is one of the important issues related to the global warming that demand for the development of drought tolerant crops. Eggplant is one of the agricultural commodities which can be developed in dry land so plant growth of eggplant need to be learned. The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, and to find the drought tolerant eggplant accessions in dry land. The experiment  was conducted at the Laboratory of Tissue Culture, Department of Agronomy and Horticulture, Bogor Agricultural University. The experiment was laid on a completely randomized design with one factor. The factor was eggplant accessions. The results showed that PEG in vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Callus in eggplant explants as a way of avoiding drought stress.

scholarly journals Physio-biochemical Responses of In Vitro Cooking Banana Musa paradisiaca cv Lang towards Pseudo Induced Drought Stress by Polyethylene Glycol (PEG)

2021 ◽  
Vol 17 (6) ◽  
pp. 805-817
Author(s):  
Sharifah Zafierah Syed Badrulzaman ◽  
Nurhana Nadia Ramlan ◽  
Munirah Adibah Kamarul Zaman ◽  
Azzreena Mohamad Azzeme
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Soluble Protein ◽  
Proline Content ◽  
Total Soluble Protein ◽  
Soluble Protein Content ◽  
Musa Paradisiaca ◽  
Cooking Banana ◽  
Total Soluble Protein Content

Musa paradisiaca cv Lang belongs to cooking banana group, and it has high potential to be used in banana chips production. Like other cultivars, M. paradisiaca cv Lang is susceptible towards water shortage, therefore affecting banana growth and productivity. In this study, to mimic the drought condition, pseudo-drought stress was given to in vitro Lang banana seedlings by adding polyethylene glycol (PEG). Overall, decrement of roots length and chlorophyll (Chl) content was displayed by the seedlings exposed to 1%, 2%, 3%, 4%, and 5% (w/v) of PEG after three weeks of exposure. The proline content, total soluble protein content, and antioxidant capacity in leaf and roots, however, countered differently towards different levels of drought. Proline content showed the highest in leaf of 2% (w/v) PEG-treated seedling (12.66±0.38 µmoles/g) while the total soluble protein content showed the highest in roots of 5% (w/v) of PEG-treated seedling (30.65±1.07 mg/g FW). Antioxidant capacity of stressed seedlings revealed the catalase (CAT), guaiacol peroxidase (POD), and ascorbate peroxidase (APX) activities were the highest in the leaf of 1% (w/v) (10.69±5.06 µmol/min/mg), 4% (w/v), (0.079±0.03 µmol/min/mg), and 5% (w/v) (9.11±8.47 µmol/min/mg) of PEG- treated seedlings, respectively. Meanwhile, the highest CAT, POD, and APX activities in the roots were determined in 3% (w/v) (0.49±0.04 µmol/min/mg), 2% (w/v) (0.03±0.02 µmol/min/mg), and 3% (w/v) (16.69±0.5 µmol/min/mg) of PEG-treated seedlings, respectively. These data show that PEG can be a priming agent to induce defense system at seedling stage of banana, which could enhance their survivability during ex vitro acclimatization.

scholarly journals Growth Response of Mucuna Cuttings to Concentration and Duration of Soaking Shallot Extract

2021 ◽  
Vol 4 (2) ◽  
pp. 122-129
Author(s):  
Enda Sabda Gentri Sembiring ◽  
Julaili Irni ◽  
Rama Riana Sitinjak ◽  
Bayu Pratomo
Keyword(s):  
Growth Response ◽  
Block Design ◽  
Duncan Multiple Range Test ◽  
Soaking Time ◽  
Extract Concentration ◽  
Survival Percentage ◽  
Randomized Block Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Range Test

Introduction: This study aims to determine the growth response of Mucuna bracteata cuttings to the concentration and duration of soaking shallot extract. Materials and Methods: This study used a factorial Randomized Block Design (RBD) with two factors, namely: shallot extract concentration (P) consists of 4 levels, include P0= untreated, P1= 10 ml, P2= 20 ml, P3= 30 ml, meanwhile the soaking time (Q) consists of 4 levels, such as Q0= momentarily dipping, Q1= 15 minutes, Q2= 30 minutes, Q3= 45 minutes. The data was processed by ANOVA and if significant, then further tested with Duncan Multiple Range Test (DMRT) at the rate of 5%. Results: The concentration of shallot extract, soaking time and their interaction had a very significant effect on the survival percentage of Mucuna bracteata D.C. cuttings. The highest survival percentage of cuttings of Mucuna bracteata on the effect of shallot extract concentration and soaking time was obtained in the P0Q0, P0Q1, and P3Q2 treatments after the lid was opened at 4 weeks after planting. In the number of leaves also had significant effect in the interaction of two factors

scholarly journals Response of in vitro propagated fig (Ficus carica L.) shoots to the concentrations of benzyl amino purine and coconut water

2021 ◽  
Vol 922 (1) ◽  
pp. 012067
Author(s):  
Sophia ◽  
M Hayati ◽  
E Kesumawati
Keyword(s):  
Callus Formation ◽  
Formation Rate ◽  
Water Concentration ◽  
Shoot Formation ◽  
Coconut Water ◽  
Contamination Rate ◽  
Two Factors ◽  
Number Of Leaves ◽  
Benzyl Amino Purine

Abstract In this study, several concentrations of benzyl amino purine (BAP) and coconut water (CW) were investigated along with the interaction between two factors to the growth of in vitro propagated fig shoots. The investigated factors consisted of BAP concentration: 0, 1, 3, 5 mg L−1 and coconut water concentration: 0, 100, 200, 300 ml L−1. A total of 16 treatment combinations with 6 replications resulting in 96 experimental units consisting of a single fig shoot explant per culture medium. The observed parameters including living explant rate, contamination rate, browning rate, day of first shoot emergence, shoot formation rate, explant height addition, number of leaves, callus formation rate, and number of roots were conducted every week from 1 to 8 weeks after proliferation (WAP). The result indicated that in 8 WAP, the living explant rate reached 23.95%. The combination of concentration 200 ml L−1 CW and 3 mg L−1 BAP + 200 ml L−1 CW-induced early emergence of new shoots at 7 days after proliferation (DAP). The highest shoot formation rate (100%) was observed at a concentration of 300 mL L−1CW. The highest explant height addition (7.10 cm) was observed at a concentration of 200 mL L−1 CW. The highest number of leaves (5.80) was observed at a concentration of 1 mg L−1 BAP + 200 mL L−1 CW. The highest callus formation rate (50%) was observed at a concentration of 100 ml L−1CW and 300 ml L−1 CW. The highest number of roots (17) was observed in the control.

scholarly journals PENGARUH NAA DAN BAP TERHADAP PERTUMBUHAN SUBKULTUR ANGGREK HASIL PERSILANGAN Dendrobium biggibum X Dendrobium liniale

2016 ◽  
Vol 31 (1) ◽  
pp. 33
Author(s):  
Sri Hartati ◽  
Agus Budiyono ◽  
Ongko Cahyono
Keyword(s):  
Plant Conservation ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Significant Difference ◽  
Two Factors ◽  
Number Of Leaves ◽  
Media Research ◽  
Test Level ◽  
Range Test

<p><em>Indonesia has a high diversity of species of orchids. One of them is the Dendrobium orchid. Orchid seeds do not have endosperm should be grown in a medium that has enough nutrients. Orchid propagation in vitro is strongly influenced by the composition of the medium used. Research through experiments aimed at studying the effect of NAA, BAP, NAA and BAP combination of the subculture plantlets growth of  </em><em>hybrids</em><em> orchid Dendrobium biggibum X liniale in Vacin Went media. Research conducted at the Laboratory of Tissue Culture Center for Plant Conservation Bogor Botanical Gardens. </em><em>The experimental design used was completely randomized design (CRD) with two factors. The first factor was the concentration of Naphthalene Acetic Acid /NAA ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). The second factor  was the type media of </em><em>Benzyl Amino Purine</em><em> /BAP ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). </em><em>Each treatment was replicated eight times. Analysis of the data by F test level 5% and if there is a significant difference continued with Duncan Multiple (Duncan's Multiple Range Test) level of 5%. The result showed that </em><em>the addition  </em><em>of NAA 3 ppm </em><em>as much as 4,96 cm </em><em> and BAP 3 ppm </em><em>as much as 4,41 cm </em><em> give a significant effect on the increase of high plantlets</em><em> </em><em>and  so NAA 3 ppm </em><em>as much as 5,76 cm </em><em> effect  on  roots length , but did not significantly effect the number of leaves and roots.</em></p>

scholarly journals Growth Induction of Cavendish Buds (Musa Acuminata L.) on Difference Concentration of IBA and BA in Vitro

2019 ◽  
Vol 8 (2) ◽  
pp. 178-187
Author(s):  
Subandi M ◽  
Arkhan Jannata ◽  
Sofiya Hasani
Keyword(s):  
Musa Acuminata ◽  
Laboratory Research ◽  
Independent Effect ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growth Induction

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.

scholarly journals The Growth Response of Several Potato Genotypes (Solanum tuberosum L.) to Induced Water Stress Using Sorbitol and Polyethylene Glycol

2016 ◽  
Vol 8 (4) ◽  
pp. 511-519 ◽  
Author(s):  
Mihaela A. CIOLOCA ◽  
Andreea M. TICAN ◽  
Maria IANOŞI ◽  
Carmen L. BĂDĂRĂU
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Drought Tolerance ◽  
Growth Response ◽  
Culture Medium ◽  
Growth Parameters ◽  
The Other ◽  
Addition Lines ◽  
In Vitro Response

The current paper aimed to study the in vitro response of potato genotypes to water stress induced by adding sorbitol and polyethylene glycol in the culture medium. The biological material analysed in the experiment was represented by a Romanian line ‘LP 11-1525/1’ and two isogenic lines ‘LI 101’ and ‘LI 102’. For cultures initiation, the line ‘LP 11-1525/1’ was started from meristems and for the other two genotypes true potato seeds were used. The studied potato genotypes behaved differently depending on the analysed parameters and on the treatment applied for drought tolerance. It was noted that the line ‘LP 11-1525/1’ achieved good results for most of the growth parameters studied, and also the lines derived from true potato seeds behaved well, in some cases even exceeding the line derived from meristems. Of the lines derived from true potato seeds, the best performance was noted for line ‘LI 101-6’ in all the analysed parameters, both on sorbitol and PEG medium. In addition, lines ‘LI 101-7’ and ‘LI 102-4’ achieved good results on both variants of medium used to mediate water stress. Therefore, establishing drought tolerance individuals within populations derived from true potato seeds using sorbitol and polyethylene glycol might be applied.

scholarly journals Regenerasi Tanaman dari Kalus Tebu yang Diiradiasi Sinar Gamma pada Medium dengan PEG 6000

2017 ◽  
Vol 44 (3) ◽  
pp. 322
Author(s):  
Agus Purwito ◽  
Nidya Ravenska ◽  
Dan Awang Maharijaya
Keyword(s):  
Drought Stress ◽  
In Vitro Selection ◽  
Gamma Ray ◽  
Peg 6000 ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Two Factors ◽  
Selection Medium ◽  
Gamma Ray Irradiation

ABSTRACT<br /><br />Sugarcane cultivars tolerant to drought stress are needed for specific location. In vitro selection can be used to obtain plants tolerant to drought stress through regeneration of callus in the culture medium. The purpose of this study was to obtain regenerants from irradiated callus on the stress medium. The experimental design was a completely randomized design with two factors. The first factor was the regeneration medium (RG) added with PEG 6000, i.e., 0%, 5%, 10% and 15%, while the second factor was the dose of gamma ray irradiation i.e. 0 krad, 5 and 10 krad, and 15 krad. There were 12 treatments, each treatment was repeated 18 times and each repetition was a culture bottles planted with three clums of callus 10 mm diameter. RG medium was the MS medium added with 0.5 mg L-1 BAP, Kinetin 0.1 mg L-1 and IBA 1.0 mg L-1. The higher the concentration of PEG, the less regenerants were produced. Regenerants could be generated from the selection medium PEG up to 15%. Several shoots still produced from callus irradiated with 10 krad. Selection medium with PEG up to 10% decreased the precentage of albino regenerants. The gamma ray irradiation dose and the concentration of PEG significanly affected the number of  roots produced.<br /><br />Keywords: drought tolerant, in vitro, polyethylene glycol, plantlets, albino<br /><br />

scholarly journals Selection and regeneration of purple sweet potato calli against drought stress simulated by polyethylene glycol

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 10
Author(s):  
Widi Sunaryo ◽  
Darnaningsih Darnaningsih ◽  
Nurhasanah Nurhasanah
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Sweet Potato ◽  
Crop Production ◽  
Callus Formation ◽  
Ms Medium ◽  
New Variant ◽  
Purple Sweet Potato

Background: Water shortage due to natural and/or technical drought stress, widespread throughout Sumatra, Java, Sulawesi and Kalimantan islands, significantly reduces crop production. The development of varieties tolerant to drought stress is important since it is more effective rather than improving irrigation infrastructure to increase the sweet potato productivity. Methods: Selection and regeneration experiments assessing purple sweet potato callus tolerance of drought stress, simulated by polyethylene glycol (PEG), were conducted to generate new variant plants tolerant of drought stress. Sterile explants (leaf and petiole) generated from previous in vitro culture were inoculated to the Murishage and Skoog (MS) medium containing plant growth regulator combination as treatments to induce calli. The calli were then transferred to half-MS medium containing 0, 5, 10, 15 and 20% PEG as selection agent for drought tolerance. The surviving calli were regenerated in the MS medium containing 0, 0.5, 1 or 1.5 mg l-1 6-benzylaminopurine (BAP). The callus formation, growth and survivability during in vitro culture were measured. Results: Calli were successfully formed in almost all media containing 2,4-Dichlorophenoxyacetic acid (2,4-D ) with the concentration of 1, 2, 3 and 4 mg l-1 and BAP (concentration: 0.5 and 1 mg l-1), but the medium of MS + 2 mg l-1 2,4-D + 0.5 mg l-1 BAP resulted in the highest number of induced calli per treatment (mean=11.36), with the percentage of responsive explants standing at around 96%. The higher the concentration of PEG, the lower the number of surviving calli. At 20% PEG, only 54.42% calli survived. There were five plants successfully regenerated from the survived calli at 20% PEG, using MS medium containing 1.5 mg l-1 BAP. Conclusions: The experiment has successfully produced putative drought-tolerant plants by callus screening using PEG as drought-tolerance-selecting agent in purple sweet potato.

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