Kersen (Muntinga calabura L) adalah pohon yang memiliki buah kecil dan manis. Tumbuhan ini dimanfaatkan antara lain sebagai obat penurun panas, mengobati pembengkakan kelenjar prostat dan mengobati penyakit asam urat, selain itu juga dapat dimanfaatkan sebagai antiseptic, antioksidan, antimikroba, dan anti inflamasi. Berbagai macam metode ekstraksi telah dikembangkan dari yang konvensional ke metode modern, dimana salah satunya yaitu Microwave Assisted Extraction (MAE) dan Ultrasound Assisted Extraction (UAE). Penelitian ini bertujuan untuk menganalisa pengaruh metode ekstraksi yang dilakukan terhadap rendemen, menganalisa pengaruh rasio solid berbanding liquid serta waktu terhadap rendemen, dan menguji kadar flavonoid yang dihasilkan dari kedua metode tersebut. Percobaan dilakukan dengan variabel rasio-pelarut 1:5 – 1:25, sedangkan variabel waktu 5 menit – 25 menit. Hasil percobaan menunjukan bahwa ke dua variabel berpengaruh terhadap konsentrasi flavonoid hasil ekstraksi. Secara umum meningkat seiring kenaikan variabel sampai maksimum di titik tertentu, kemudian turun. Konsentrasi maksimum diperoleh pada variabel umpan-pelarut MAE sebesar 1:25 dengan kadar flavonoid 132,41 mg/ml dan rendemen sebesar 39%. Adapun waktu terbaik adalah 5 menit dengan kadar flavonoid 91,669 mg/ml dan rendemen sebesar 22,7%. Sedangkan pada proses UAE (Ultrasonic Assisted Extraction) dengan rasio umpan-pelarut maksimal sebesar 1:10 dengan kadar flavonoid 47,5899 mg/ml dan rendemen 26%. Sedangkan waktu terbaik 10 menit dengan kadar flavonoid 56,7769 mg/ml dan rendemen sebesar 19,65%. AbstracKersen (Muntinga calabura L) is a tree with small and sweet fruit. The fruit is used, among others, as a febrifuge, treating swelling of the prostate gland and treating gout. Besides the fruit, its leaves also has potential as an antiseptic, antioxidant, antimicrobial, and anti-inflammatory. Various extraction methods have been developed from conventional to modern methods, one of which is Microwave Assisted Extraction (MAE) and Ultrasound Assisted Extraction (UAE). The research will extract the kersen leaves and aim to analyze the effect of the extraction method on the yield, the effect of the solid to liquid ratio and time on the yield, and the flavonoid content produced by both methods. The experiment was carried out with a solvent-ratio variable of 1:5 – 1:25, while the time variable was 5 – 25 minutes. The results of the experiment showed that the two variables had an effect on the concentration of flavonoids extracted. In general, it increases as the variables increase to a maximum point, then decreases. The maximum concentration obtained in the feed-solvent variable MAE was 1:25 with a flavonoid content of 132.41 mg/ml, with a yield of 39% and the best time was 5 minutes with a flavonoid content of 91.669 mg/ml, with a yield of 22.7%. While in the UAE Ultrasonic Assisted Extraction process with a maximum feed-solvent ratio of 1:10 with a flavonoid content 47.5899 mg/ml and yield 26%, while the best time of process was 10 minutes with flavonoid content 56.7769 mg/ml and yield 19, 65%.Kata kunci: Flavonoid, Ekstrak Kersen, MAE, UA
Cytotoxicity of Begonia medicinalis aqueous extract in three cancer cell line
