scholarly journals Ecological features of the accumulation of carotenoids in the Black Sea molluscs

2021 ◽  
Vol 937 (2) ◽  
pp. 022077
Author(s):  
A Borodina ◽  
P Zadorozhny

Abstract The analysis of carotenoids of three species of bivalve filter feeding molluscs: Cerastoderma glaucum, Chamelea galina and Polititapis aureus, inhabiting the same ecotope, in the innermost part of the bay of the Sevastopol city, has been carried out. The dynamics of total carotenoid content (TCC) in these species had shown during 4 seasons of the year. The composition of carotenoids is presented in C. glaucum and P. aureus by 8 carotenoids, and Ch. galina - 11 carotenoids. However, in P. aureus, only 75% of the TCC were analyzed. The TCC of all 3 species of molluscs contained to 75% of the total (identical) carotenoids. Each species of molluscs retained their specific carotenoids. Comparative analysis of carotenoids between Black Sea mollusks and their species analogues from other regions of the World Ocean showed differences in the composition of carotenoids. Thus, changing environmental conditions of the same type of filter-feeding mollusk lead to the implementation of adaptation mechanisms in the body, as a result of which the qualitative composition of carotenoids changes.

Author(s):  
A. K. Veligodska ◽  
O. V. Fedotov ◽  
A. S. Petreeva

<p>The influence of certain nitrogen compounds - components of glucose-peptone medium (GPM) on the accumulation of carotenoids by some strains was investigated by surface cultivating basidiomycetes. The total carotenoid content was set in acetone extracts of mycological material spectrophotometrically and calculated using the Vetshteyn formula.</p> <p>As the nitrogen-containing components used GPM with 9 compounds, such as peptone, DL-valine, L-asparagine, DL-serine, DL-tyrosine, L-proline, L-alanine, urea, NaNO<sub>3</sub>. The effect on the accumulation of specific compounds both in the mycelium and in the culture fluid of carotenoids by culturing certain strains of Basidiomycetes was identified.</p> <p>Adding to standard glucose-peptone medium peptone at 5 g/l causes an increase of carotenoid accumulation by strain <em>L. sulphureus</em> Ls-08, and in a concentration of 4 g/l by strains of <em>F. hepatica </em>Fh-18 and <em>F. fomentarius</em> Ff-1201.</p> <p>In order to increase the accumulation of carotenoids in the mycelium  we suggested to make a standard glucose-peptone medium with proline or valine for cultivating of <em>L. sulphureus</em> Ls- 08 strain; alanine for <em>F. fomentarius</em> Ff-1201 strain; proline, asparagine and serine - for strain Fh-18 of <em>F. hepatica</em>. The results can be implemented in further optimization of the composition of the nutrient medium for culturing strains of Basidiomycetes wich producing carotenoids.</p> <p><em>Keywords: nitrogen-containing substances, Basidiomycetes, mycelium</em><em>,</em><em> culture filtrate, carotenoids</em></p>


Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 721
Author(s):  
Kristina Kljak ◽  
Klaudija Carović-Stanko ◽  
Ivica Kos ◽  
Zlatko Janječić ◽  
Goran Kiš ◽  
...  

The aim of this study was to evaluate the effect of a supplementation diet for hens consisting of dried basil herb and flowers of calendula and dandelion for color, carotenoid content, iron-induced oxidative stability, and sensory properties of egg yolk compared with commercial pigment (control) and marigold flower. The plant parts were supplemented in diets at two levels: 1% and 3%. In response to dietary content, yolks from all diets differed in carotenoid profile (p < 0.001). The 3% supplementation level resulted in a similar total carotenoid content as the control (21.25 vs. 21.79 μg/g), but by 3-fold lower compared to the 3% marigold (66.95 μg/g). The tested plants did not achieve yolk color fan values as the control (13.47) or 3% marigold (11.47), and among them, calendula had the highest values (9.73). Despite the low carotenoid content in diets supplemented with basil herb, iron-induced malondialdehyde (MDA) concentration was low as for marigold (on average 106.83 vs. 92.68 ng/g after 250 min). The treatments differed in sensory color scores for fresh and hard-boiled yolks and flavor while other sensory properties were similar. In conclusion, the supplementation of plants in a hen diet may result in yolks containing carotenoids and other compounds showing a high antioxidant effect.


2021 ◽  
Vol 66 (3) ◽  
Author(s):  
Alexandra Saranchina ◽  
Polina Drozdova ◽  
Andrei Mutin ◽  
Maxim Timofeyev

Proper diet is critical for laboratory-reared animals, as it may affect not only their welfare, but also experimental results. Amphipods (Crustacea: Amphipoda) play important roles in ecosystems and are often used in environmental research. Endemic amphipods from the ancient Lake Baikal are promising for laboratory bioassays; however, there are currently no laboratory cultures. In this work, we determine how different diets affect the color and metabolism of a laboratory-reared Baikal amphipod, Eulimnogammarus cyaneus. We found that in freshly collected blue-colored animals, body color correlated with total carotenoid content. Total carotenoid levels did not differ after long-term (two months) feeding with a close to natural carotenoid-enriched, or even a carotenoid-depleted diet. Nevertheless, antennae color was closer to red in the natural-like diet group. It is likely that the carotenoids from the commercial diet are not properly metabolized in E. cyaneus. The animals fed commercial diets had a higher glycogen content, which may signify a higher metabolic rate. Overall, we show that a carotenoid-enriched diet optimized for decapods is not optimal for amphipods, likely due to different carotenoid compositions, and the diet for long-term rearing of E. cyaneus and other Baikal amphipods requires supplementation.


Zootaxa ◽  
2021 ◽  
Vol 5060 (2) ◽  
pp. 33-64
Author(s):  
SEVGI KUŞ ◽  
GÜLEY KURT ◽  
MELIH ERTAN ÇINAR

The present paper deals with the diversity of nephtyid polychaetes (Nephtyidae) from the Sea of Marmara and the Black Sea. Three species belonging to two genera (Micronephthys and Nephtys) were found in the Black Sea (coast of Turkey) and six species belonging to three genera (Inermonephtys, Micronephthys and Nephtys) were found in the Sea of Marmara. The material includes two species new to science, Inermonephtys turcica n. sp. and Nephtys sinopensis n. sp., and a species record (Nephtys kersivalensis McIntosh, 1908) new to the Sea of Marmara’s marine fauna. Nephtys sinopensis n. sp. is mainly characterized by having 1–4 geniculate chaetae in the postacicular position of the parapodia; digitiform antennae, palps, and ventral cirri at chaetiger 1 with swollen tips; small and cirriform branchiae present from chaetiger 4 to the end of the body; poorly developed parapodial prechaetal lamellae in median and posterior chaetigers and long ventral cirri along the body. Inermonephtys turcica n. sp. is mainly characterized by having cushion-like palps with digitiform tips; well developed neuropodial postchaetal lamellae; barred chaetae in preacicular position of the anterior and median parapodia; and branchiae first appearing between chaetiger 3 and 13 (depending on body size).  


2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Mohammed Bourhia ◽  
Hamza Elmahdaoui ◽  
Samir Iben Moussa ◽  
Riaz Ullah ◽  
Ahmed Bari

Background. Barbary fig (Opuntia spp), called prickly pear, is a cultivated species belonging to family Cactaceae. It produces fruits one time per year during a short season. It is largely spreading in the Mediterranean countries. The aim of the study was to investigate the physicochemical and biochemical analysis of powder of prickly pear fruit peels of three Moroccan varieties (Aakria, Derbana, and Mles). Material and Methods. Both physicochemical analysis (humidity, water activity, Brix, ash content, pH, and total titratable acidity) and biochemical analysis (total carotenoid content, betalain content, total polyphenolic content, and ascorbic acid content) were effectuated according to protocols reported within the present research work. Results. Regarding the physiochemical analysis, the humidity of powder of prickly pear fruit peels ranged from 10.173 ± 0.002 to 15.27 ± 0.02%. The water activity (aw) ranged from 0.17 ± 0.002 to 0.336 ± 0.002. The values of Brix ranged from 68.67 ± 0.33 to 74.22 ± 0.51° Bx. pH values ranged from 5.41 ± 0.01 to 5.62 ± 0.01. The total titratable acidity values ranged from 0.644 ± 0.014 to 0.76 ± 0.007 g citric acid/100 g DM (dry matter). The ash content ranged from 1.02 ± 0.02 to 11.41 ± 0.03 g/100 g DM. Regarding the biochemical analysis, the total carotenoid content ranged from 5.14 ± 0.10 to 9.79 ± 0.06 mg/g of FM (Fresh matter). The total betalain content ranged from 2 ± 0.69 to 37.66 ± 2.65 mg/100 g of DM. The total polyphenolic content ranged from 1739.92 ± 13.69 to 2409.66 ± 43.65 mg gallic acid equivalent (GAE)/100 g of DM. The ascorbic content values ranged from 186.78 ± 13.23 to 294.04 ± 5.81 mg/100 g of DM. Conclusion. The results showed an interesting richness of the investigated powder in pigments (betacyanins, indicaxanthins, and carotenoids) and nutritional compounds including sugar, proteins, and vitamins that make this powder interesting for being used as a dye in food.


Crustaceana ◽  
2014 ◽  
Vol 87 (11-12) ◽  
pp. 1393-1410 ◽  
Author(s):  
F. Üstün ◽  
T. Terbiyik Kurt ◽  
E. Suárez-Morales

Cymbasoma sinopense sp. nov. is described from an adult female collected off the Sinop coast, in the southern Black Sea, Turkey. This is the first species of this genus recorded from the Black Sea coast of Turkey. The new species is a member of the widespread Cymbasoma longispinosum species-group, and it closely resembles C. chelemense Suárez-Morales & Escamilla, 1997 from the Yucatan Peninsula and C. californiense Suárez-Morales & Palomares-García, 1999 from the Gulf of California. This species can be distinguished by a combination of subtle characters, including the body proportions, the structure and armature of the fifth legs, the ornamentation of the genital somite, and the relative length of the ovigerous spines. A previous report of C. longispinosum from the northern Black Sea probably represented a misidentified record of C. sinopense sp. nov. The Mediterranean reports refer to a species that is different from the one of the Black Sea. Comparative comments and data including the main taxonomic characters of members of the Cymbasoma longispinosum species-group are presented. Overall, it is confirmed that records of this species from different geographic areas should be revised carefully because they could represent undescribed species. A key for the identification of the females of the currently known species assigned to the Cymbasoma longispinosum species-group is included.


2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Mohammed Bourhia ◽  
Hamza Elmahdaoui ◽  
Riaz Ullah ◽  
Ahmed Bari ◽  
Laila Benbacer

Background. Prickly pear (Opuntia spp.), called Barbary fig, is a cultivated species springing from family Cactaceae. It is native to Mexico and has been naturalized in other continents, especially the Mediterranean countries (North Africa). The aim of the study was to investigate the physical, physicochemical, and biochemical criteria of peels of three Moroccan prickly pear varieties (Aakria, Derbana, and Mles) growing in the Rhamna regions (dry area). Material and Methods. Both physicochemical characteristics (humidity, water activity, Brix, ash content, pH, and total titratable acidity) and biochemical characteristics (total carotenoid content, betalain content, total polyphenolic content, and ascorbic acid content) were were studied according to previously reported methods. Results. Regarding the physiochemical criteria, the moisture of the fresh peels of studied varieties ranged from 81.59 ± 0.02 to 83.47 ± 0.02%. The water activity (aw) ranged from 0.862 ± 0.001 to 0.872 ± 0.001. The values of Brix varied from 14.69 ± 0.05° Bx to 15.80 ± 0.03° Bx. pH values varied from 5.13 ± 0.01 to 5.32. The total titratable acidity values ranged from 0.130 ± 0.008 to 0.196 ± 0.014 g of citric acid/100 g of FM (fresh matter). The ash content values ranged from 8.92 ± 0.10 to 11.04 ± 0.06 g/100 g of FM. Regarding the biochemical criteria, the total carotenoid content ranged from 2.29 ± 0.01 to 2.87 ± 0.01 μg/g of FM. The total betalain content ranged from 6213.46 ± 58.86 to 8487.19 ± 51.71 μg/100 g of FM. The total polyphenolic content varied from 160 ± 3.55 to 243.79 ± 5.55 mg GA E/100 g of FM. The ascorbic content ranged from 58.21 ± 0.24 to 74.72 ± 0.17 mg/100 g of FM. Conclusion. The findings of physicochemical and biochemical criteria of the investigated varieties growing in Moroccan drylands showed promising results in terms of studied parameters.


2012 ◽  
Vol 24 (1) ◽  
pp. 33-39
Author(s):  
Wanda Wadas ◽  
Halina Mioduszewska ◽  
Krzysztof Kalinowski

Effects of selected agronomical factors on the content of health-promoting compounds in spaghetti squash (Cucurbita pepoL. subsp.pepo)The study examined the effects of the sowing date (5, 15 and 25 May) and plant spacing (1 × 0.6 m, 1 × 0.8 m and 1 × 1 m) on the content of carotenoids and L-ascorbic acid in the fruits of two cultivars of spaghetti squash (‘Makaronowa Warszawska' and ‘Pyza’). The field experiment was carried out in central-eastern Poland. Fully mature spaghetti squash fruits were harvested from the beginning of September to the beginning of October. The content of total carotenoids and β-carotene was the greatest in the fruits of plants sown on 15 May. The sowing date did not affect the content of L-ascorbic acid. Increasing the plant spacing from 1 × 0.6 m to 1 × 1 m resulted in an increase of total carotenoid content, particularly for the spaghetti squash sown on 25 May. Plant spacing did not have a significant effect on the content of β-carotene and L-ascorbic acid. Fruits of the ‘Makaronowa Warszawska’ with light orange flesh contained more total carotenoids and β-carotene than fruits of the ‘Pyza’ with cream-coloured flesh. The content of L-ascorbic acid in the fruits of both cultivars did not differ significantly.


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