scholarly journals Investigation of the bioactivities of extracts from Vernonia Amygdalina Del

2021 ◽  
Vol 947 (1) ◽  
pp. 012040
Author(s):  
Nga Phan Thi Thanh ◽  
Thanh Truc Tran
Keyword(s):  
Survival Rate ◽  
Cancer Cells ◽  
Inhibitory Concentration ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dpph Assay ◽  
Vernonia Amygdalina ◽  
Gram Positive Bacteria ◽  
Dpph Radical Scavenging

Abstract The antibacterial, antioxidant activity, and anticancer of the extract from Vernonia Amygdalina Del leaves, was assessed towards 6 selected bacteria, in the DPPH test, as well as on hepatic (HepG2), blood (K562) cancer cell lines. The DPPH assay revealed that the highest DPPH radical scavenging activity with the warm ethanol extract (IC50 = 259,03 ± 5,42 μg/mL). And the ethyl acetate extract showed a high antibacterial effect expressed as minimum inhibitory concentration (MIC = 100 mg/mL) against Gram-positive bacteria. At the concentration of 250 mg/mL, this extract also demonstrated its ability to resist blood cancer cells K562 (with a survival rate of 25%) and liver cancer cells HepG2 (with a survival rate of 0%).

scholarly journals ANTIOXIDANT ACTIVITY OF RHIZOME EXTRACTS OF COLEUS FORSKOHLII BRIQ

2018 ◽  
Vol 11 (11) ◽  
pp. 275 ◽  
Author(s):  
Shanmugam S ◽  
Usha Rani V ◽  
Pradeep B V
Keyword(s):  
Antioxidant Activity ◽  
Tamil Nadu ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Coleus Forskohlii ◽  
Phytochemical Constituents ◽  
Polar Extracts ◽  
Dpph Radical Scavenging

Objective: The present study was on the analysis of the phytochemical constituents of Coleus forskohlii which was collected from five different areas in Tamil Nadu, where it is grown for commercial purposes.Methods: C. forskohlii tubers were collected from five areas (Thiruvannamalai, Kanchipuram, Kallakuruchi, Salem, and Vandalur) of Tamil Nadu. Dried tubers were extracted with different solvents such as aqueous, ethanol, and acetone (polar) and petroleum and chloroform (non-polar). Extracts were evaluated for total phenol and flavonoid content and antioxidant activity evaluation by 1,1-diphenyl-2-picrylhydrazyl (DPPH) reduction.Results: The highest DPPH radical scavenging activity (87.6%) was observed in ethanol extract of rhizome collected from Thiruvannamalai area. Total phenol and flavonoid contents were found to be, respectively, 38.82±0.22 mg gallic acid equivalents/g and 21.34±0.32 quercetin equivalent/g.Conclusion: The study clearly indicated the antioxidative potential of different extracts of C. forskohlii and this property may be useful in the preparation of drug formulations, health tonics, and cosmetics.

STUDY OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF SAXICOLOUS LICHEN $ \textit{XANTHOPARMELIA STENOPHYLLA} $

2020 ◽  
Vol 54 (2 (252)) ◽  
pp. 132-137
Author(s):  
A.G. Simonyan ◽  
R.R. Sargsyan ◽  
H.H. Panosyan ◽  
A.H. Trchounian
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Gram Positive Bacteria ◽  
Acetone Extracts ◽  
Dpph Radical Scavenging

Antibacterial and antioxidant activities of different extracts of saxicolous lichen Xanthoparmelia stenophylla sampled from Norashen, Gegharkunik Province of Armenia were studied. Methanol, ethanol and acetone extracts of lichen thalli were demonstrated to have activity against only tested gram-positive bacteria. Methanol extract of the lichen showed the highest amount of DPPH radical scavenging activity (~68%). Our studies did not reveal any significant antibacterial and antioxidant activities of aqueous extract.

scholarly journals Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods

2020 ◽  
Vol 8 (12) ◽  
pp. 2749-2755
Author(s):  
Mohammad Afzal Hossain ◽  
Nadia Khan Disha ◽  
Jahid Hasan Shourove ◽  
Pappu Dey
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Tannin Content ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Moringa leaves were oven-dried and powdered to determine the antioxidant activity in terms of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity and total tannin content. The maximum TPC value noted in methanol extract, and it ranges from 6.46 ± 0.34 g to 3.91±0.19 g gallic acid equivalent (GAE)/100g dry sample (DM). The FRAP values varied from 211.6 ± 3.75 to 344.13 ± 3.26 mg ascorbic acid equivalent (AAE)/100g DM and 166.67 ± 2.90 to 224.93 ± 1.94 mg AAE/100g DM, for methanol and ethanol extract, respectively. The DPPH radical scavenging activity ranged from 46.32 ± 1.07 to 58.09 ± 0.92% for methanol extract and 56.76 ± 1.48% to 69.72 ± 1.15% for ethanol extract. The total tannin content varied from 6.84 ± 0.05 to 10.22 ± 1.11 mg GAE/100 g dry sample and 6.77 ± 0.08 to 9.23 ± 0.51 mg GAE/ 100g DMfor methanol and ethanol extract, respectively. The highest antioxidant yield for methanol and ethanol extract of Moringa leaves differed for various conditions. Overall, Moringa leaves showed excellent antioxidant properties that can be commercially and domestically used.

scholarly journals EFFECT OF STERCULIA QUADRIFIDA R. BR BARK TO IN VITRO DPPH RADICAL AND GLUTATHIONE PEROXIDASE ON DIABETIC WISTAR RATS

2020 ◽  
pp. 128-131
Author(s):  
RAMBU KONDA A PRAING ◽  
TITIK SUNARNI
Keyword(s):  
Antioxidant Activity ◽  
Glutathione Peroxidase ◽  
Wistar Rats ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Gpx Activity

Objective: The aims of the research were to evaluate the antioxidant effect of Sterculia quadrifida R.Br bark by in vitro 1,1-biphenyl-2-picryl-hydrazyl (DPPH) radical scavenging method and glutathione peroxidase (GPx) activity on diabetic Wistar rats model. Methods: S. quadrifida bark powder was extracted in ethanol 70% by maceration method. The antioxidant activity of ethanol extract was tested by DPPH radical scavenging method. The activity of GPx was evaluated from the liver of alloxan-induced diabetic rats. The antioxidant activity was determined by measuring the absorbance using ultraviolet-visible spectrophotometry method. Results: The ethanol extract of S. quadrifida bark had a strong DPPH radical scavenging activity, with the IC50 value was 4.86±0.01 ug/ml, besides that the extract also showed significantly activity of GPx (p<0.05) at a dose of 65, 130, and 260 mg/kg bw. Conclusion: The ethanol extract of S. quadrifida bark had a potent antioxidant activity by DPPH radical scavenging and GPx activity.

scholarly journals DMFDMA catalyzed synthesis of 2-((Dimethylamino)methylene)-3,4-dihydro-9-arylacridin-1(2H)-ones and their derivatives: in-vitro antifungal, antibacterial and antioxidant evaluations

2018 ◽  
Vol 16 (1) ◽  
pp. 1077-1088
Author(s):  
L. Jyothish Kumar ◽  
S. Sarveswari ◽  
V. Vijayakumar
Keyword(s):  
Antibacterial Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gram Negative Bacteria ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Gram Positive Bacteria ◽  
Dpph Radical Scavenging ◽  
Mic Value

AbstractA series of 3,4-dihydro-9-arylacridin-1(2H)-ones was synthesized and enaminone function was introduced at the C-2 position using DMFDMA catalyst which in turn successfully converted into pyrazole, isoxazol, 1-phenyl-1H-pyrazole by treating it with reagents such as hydrazine, hydroxylamine and phenylhydrazine. These newly synthesized compounds were evaluated for their antibacterial activity against a series of Gram-Positive bacteria including Staphylococcusaureus, Bacilluscereus, StaphylococcusaureusMLS16 and Gram-Negative bacteria including Klebsiellaplanticol, Escherichiacoliand Pseudomonasaeruginosaand also against fungal strains including Candidaalbicans, Candidaparapsilosis, Candidaglabrata, Candidaaaseri, Aspergillusnigerand Issatchenkiahanoiensis. The compounds3aand6aexhibited considerable antifungal activity (MIC value 0.007 and 0.006 μM) against Candidaalbicansand Aspergillusnigerrespectively. The compound4ashowed excellent antibacterial activity towards EscherichiaColi(MIC = 0.003 μM) and the compound5afound to show prominent DPPH radical scavenging activity with EC50value 16.85±1.5μg mL−1.

scholarly journals Chemical Composition, Antioxidant, Antimicrobial, and α-Glucosidase Activities of Essential Oils of Hornstedtia scyphifera (Zingiberaceae)

2018 ◽  
Vol 13 (2) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Siti Ernieyanti Hashim ◽  
Hasnah Mohd Sirat
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Moderate Activity ◽  
Gram Positive Bacteria ◽  
Germacrene D ◽  
Leaf Oil ◽  
Inhibition Zones ◽  
First Time ◽  
Dpph Radical Scavenging ◽  
Β Carotene

The essential oil compositions of the rhizomes, flowers, leaves, and pseudostems of Hornstedtia scyphifera (J. Koenig) Steud were studied for the first time. A total of forty three constituents were identified from the rhizome, flower, leaf, and pseudostem oils, representing 90.9%, 82.4%, 79.7%, and 82.6%, respectively. α-Selinene was found dominant in the flower (10.8%) and pseudostem (12.4%) oils, while the rhizome and leaf oils contained germacrene D (15.5%) and camphor (12.6%) as the main constituents, respectively. The rhizome, flower, and leaf oils showed moderate activity against Gram positive bacteria with MIC values of 225 to 450 μg/mL and inhibition zones of 8.4 to 10.0 mm. The leaf oil showed good β-carotene bleaching inhibition activity (64.8%) and a low IC50 value for DPPH radical scavenging activity (650.4 μg/mL). The flower oil showed potential α-glucosidase inhibitory activity with an IC50 value of 21.44 μg/mL.

scholarly journals Antimicrobial and antioxidant property of Curcuma longa Linn.

2019 ◽  
Vol 8 (11) ◽  
pp. 2383 ◽  
Author(s):  
Intan Athirah Muhamed ◽  
Wan Amir Nizam Wan Ahmad ◽  
Nurul Syahida Ramli ◽  
Noraini A. Ghafar
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Butylated Hydroxytoluene ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

Background: Curcuma longa Linn. (Zingiberaceae family) plant or turmeric is known to possess pharmacological properties at relatively nontoxic doses. The study was carried out with the aim to evaluate the antimicrobial and antioxidant activities of C. longa L.Methods: Ethanolic rhizome extract was applied against Staphylococcus aureus and Salmonella typhi using disc and well diffusion methods. Antioxidant of C. longa L. was determined by measuring 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity.Results: The ethanolic extract of rhizomes showed inhibitory activity against Gram-positive S. aureus bacteria, whereas the Gram-negative S. typhi bacteria were resistant toward the ethanol extract. Zone of inhibitions against S. aureus were 7 mm and 11 mm by disc and well method, respectively. For antioxidant activity, DPPH radical scavenging activity of ethanolic extract of C. longa L. was set against butylated hydroxytoluene (BHT). It was shown that the value of half maximal inhibitory concentration (IC50) of rhizome extract was higher than standard control. The dose response curve was utilized to determine the half maximal IC50 value for each sample. The IC50 of rhizome extract is 21.25 µg/ml, while the value IC50 for BHT is 12.42 µg/ml.Conclusions: The finding suggested turmeric rhizome-extract as a good candidate in the search for a natural antimicrobial and antioxidant agent.

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF ALKALOID EXTRACT OF CUCUMIS TRIGONUS ROXB

2019 ◽  
pp. 44-48
Author(s):  
PRATIMA H
Keyword(s):  
Antibacterial Activity ◽  
Superoxide Radical ◽  
Inhibitory Concentration ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Peroxide Radical ◽  
Scavenging Activity ◽  
Alkaloid Extracts ◽  
Dpph Radical Scavenging

Objective: To evaluate the antioxidant and antibacterial efficiency of alkaloids of root, leaf, and fruit of Cucumis trigonus. Methods: The antioxidant and antibacterial properties of alkaloid extracts were assessed by ferric thiocynate (FTC), 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, hydrogen peroxide radical scavenging assay, superoxide radical scavenging activity and ferric reducing power were analyzed separately for its inhibition percentage at different concentration (20µg, 30µg, 40µg and 50µg/ml) and antibacterial activity by agar disc diffusion and minimum inhibitory concentration (MIC) against Staphylococcus aureus (MTCC Code-9886), Pseudomonas aeruginosa (MTCC Code-6458). Results: The highest percentage of extraction yield was observed in fruit followed by leaf and root. The maximum content of alkaloid was found in fruit and root compared to leaf. The scavenging efficiencies of the extracts increased with the increasing concentrations. Appreciable levels of total antioxidant activity by FTC (2.112±0.011%), DPPH radical scavenging activity (1.912±0.001%), superoxide radical scavenging activity (0.955±0.021%) and hydrogen peroxide radical scavenging activity (0.914±0.087 %) were observed at 50µg/ml of fruit extract. However, ferric reducing power was more in the root (0.893±0.0870%) compared to fruit (0.791±0.023%) and leaf (0.520±0.00 %) at 50µg/ml. The alkaloid extracts of root and fruit exhibited more antibacterial activity against Staphylococcus aureus (16.51±0.05 and 19.68±0.03 mm) and Pseudomonas aeruginosa (12.2±0.03 and14.2±0.06) at 100µg/ml than leaf. The minimum inhibitory concentration (MIC) of the root, leaf, and fruit was in the range of 3.125µg/ml to 35µg/ml for the pathogenic bacteria. Conclusion: Data from the present results revealed that the alkaloid extracts of fruit and root of C. trigonus show good antioxidant and antibacterial potential than leaf. Hence, may be explored for the formation of new antibacterial with antioxidant drugs.

Immune enhancement effects and extraction optimization of polysaccharides from Citrus aurantium L. var. amara Engl.

2017 ◽  
Vol 8 (2) ◽  
pp. 796-807 ◽  
Author(s):  
Chun-Yan Shen ◽  
Li Yang ◽  
Jian-Guo Jiang ◽  
Chao-Yang Zheng ◽  
Wei Zhu
Keyword(s):  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Citrus Aurantium ◽  
Human Breast ◽  
Dpph Radical Scavenging Activity ◽  
Immune Enhancement ◽  
Dpph Radical Scavenging ◽  
Mcf 7

The crude polysaccharides ofCitrus aurantiumL. var.amaraEngl (CAVAPs) were extracted and their bioactivities including DPPH radical scavenging activity, cytotoxicity to human breast cancer cells, MCF-7, as well as lung cancer cells, HCC827, and their immune-enhancement activity were evaluated.

scholarly journals Phytochemical Screening and Antioxidant Analysis of the Ethanolic Extract of Rosehip Seed Press Cake

2020 ◽  
pp. 57-67
Author(s):  
E. B. Tanor ◽  
R. P. Matamane ◽  
I. Hapazari ◽  
S. Magama
Keyword(s):  
Press Cake ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Food Supplement ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Dpph Radical Scavenging Activity ◽  
Semi Solid ◽  
Dpph Radical Scavenging

Aims: Rosehip seed press cake is a waste material in the industrial extraction of rosehip seed oil using cold press process. The current study seeks to evaluate the phytochemical profile, total phenolic content, total flavonoid content and DPPH radical scavenging activity of the ethanolic extract of rosehip seed press cake using standard methods and DPPH assay. Study Design:  This is an experimental laboratory report on the phytochemical properties and antioxidant potential of the ethanolic semi-solid extract (ESE) of rosehip seed press cake in order to assess its commercial viability as a food supplement. Place and Duration of Study:  The work was conducted in the Department of Chemistry, National University of Lesotho, from August 2019 to March 2020.  Methodology: Ethanol was used for the extraction of the semi-solid extract (ESE) from the rosehip seed press cake. The ESE was analysed for phytochemical constituents using standard methods. In vitro antioxidant activity of the ESE was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. For data analysis, mean and standard deviation were computed for each parameter that was quantitatively determined. Results: Presence of flavonoids, alkaloids, terpenoids, sterols, glycosides, reducing sugars, proteins, amino acids, fatty acids, phenols and polyphenols in the ESE was confirmed. Total phenolics and flavonoids content were found to be 134.44 mg GAE/g DW and 73.23 mg QE/g DW, in the range of 200 to 3000 µg/ml respectively. The DPPH radical scavenging activity of the extract was found to be in the range 10.32±3.89 and 76.06±3.48% within the concentration range and was very close to the scavenging activity of DPPH at concentrations ≥ 1500 µg/ml. The ESE showed an IC50 value of 1367.06 µg/ml relative to that of the positive control, ascorbic acid, being <200 µg/ml. Conclusion: The ESE from the rosehip seed press cake was found to be very promising as a food supplement since 50% of the 16 screened phyto-constituents were present in high concentrations; 6.25% were detected in moderate concentrations and another 6.25% were present in low concentrations. Furthermore, the ESE exhibited antioxidant properties. Further studies are recommended to obtain more information on its composition and suitability additive in animal feed or as a food supplement.

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