scholarly journals Morphology, histochemical test, potential, and conservation effort of Alpinia warburgii K. Schum., a native species to Sulawesi

2021 ◽  
Vol 948 (1) ◽  
pp. 012014
Author(s):  
Trimanto ◽  
L Hapsari
Keyword(s):  
Native Species ◽  
Cell Structure ◽  
Morphological Characteristics ◽  
Anatomical Study ◽  
Anatomical Structure ◽  
Ex Situ ◽  
Secretory Cells ◽  
Vascular Bundles ◽  
Conservation Effort ◽  
Histochemical Tests

Abstract Alpinia warburgii is a species of the Zingiberaceae family native to Sulawesi. This study aims to characterize the plant morphology; analyze the anatomical structure, histochemical localization, and secretory cell structure of the rhizome; also discuss the potential and conservation effort of the species. Anatomical study and histochemical tests were observed microscopically using the fresh preparation method and wet mounted slide. Histochemical tests carried out include starch, protein, lipid, tannin, alkaloid, and flavonoid. This study is considered the first report of histochemical tests on A. warburgii. The main morphological characteristics of A. warburgii are perennial herbaceous; inflorescence terminal and raceme; flowers white, labellum obovate with red lines in the middle, and flanked by light green edges; fruit globose, green when young and yellow when ripe. The rhizome anatomical structure comprises the epidermis, endodermis, hypodermis, parenchyma, cortex, and vascular bundles, classified as closed collateral type. Histochemical tests showed positive localization for starch, protein, alkaloids, flavonoids, and tannins. The density of flavonoids secretory cells is higher than tannins and alkaloids, which indicates its potential as medicine to be associated with antioxidant, antimicrobial, antiinflammation, and anticancer. In addition to in-situ, ex-situ conservation effort through propagation is also important to maintain the species.

scholarly journals Struktur Anatomi dan Uji Histokimia Terpenoid dan Fenol Dua Varietas Sirih Hijau (Piper betle L.)

BIOSCIENTIAE ◽  
2021 ◽  
Vol 17 (2) ◽  
pp. 1
Author(s):  
Gusti Puspa Dewi ◽  
Evi Mintowati Kuntorini ◽  
Eny Dwi Pujawati
Keyword(s):  
Copper Acetate ◽  
Anatomical Structure ◽  
Secretory Cells ◽  
Cell Diameter ◽  
Piper Betle ◽  
Vascular Bundles ◽  
Palisade Parenchyma ◽  
Betel Leaf ◽  
Epidermal Tissue ◽  
Pith Parenchyma

This study aims to determine the anatomical structure and histochemical test of terpenoid and phenol compounds in two varieties of green betel plants (Piper betle). Making leaves anatomical structure preparations using the fresh method, testing terpenoid compounds with 5% copper acetate, testing phenol with ferric trichloride 10% and some grains of sodium carbonate. The observations of the anatomical structure of green betel leaf varieties 1 and varieties 2 have similarities consisting of the upper epidermis, upper hypodermis, palisade parenchyma, parenchymal sponges, vascular bundles (xylem and phloem), sclerenchyma, cholenchyma, lower epidermis, lower hypodermis, secretory cells, trichoma, stoma and calcium oxalate crystals, and in varieties 2 look more trichomes. The anatomical structure of the variety 1 betel stem and varieties 2 are arranged from the outside in the direction of the epidermal tissue, colenchymal tissue, cortical bundles, sclerenchyma, cortex, medullary and peripheral vascular files, pith, the central part of the stem is a secretory gland. Phenol in betel vine varieties 1 and varieties 2 is positive in the secretion cell part which is spread in the parenchymal tissue of the mother's leaf bone and lamina, whereas in the stem is spread around the cortex and pith parenchyma. Positive secretion cells contain phenol not as much as secretory cells containing terpenoids. Based on quantitative observations the size of oil cell density and secretion cell diameter, the essential oils contained in the cell secretions in the leaves of variety 1 are more than varieties 2 while in the varieties 2, there are more varieties 1.

scholarly journals Secrets beneath the soil: recovery of fern spores as a strategy of biodiversity conservation in Punta Lara Nature Reserve (PLNR), Argentina

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Pedro Cayetano Berrueta ◽  
Maria Luján Luna ◽  
Gabriela Elena Giudice ◽  
Marta Mónica Ponce
Keyword(s):  
Native Species ◽  
Nature Reserve ◽  
In Situ Conservation ◽  
Morphological Characteristics ◽  
Ex Situ ◽  
Natural Environments ◽  
Local Species ◽  
Soil Recovery

Abstract The recovery of soil spores is a strategy to strengthen in decline or disappeared populations from natural environments. In this work, we analyzed 25 soil samples extracted from a patch of gallery forest in an “albardón” of Punta Lara Reserve, Buenos Aires. The samples were distributed in 50 Petri dishes, 25 exposed to controlled temperature and light and another 25 kept in darkness. To contribute to the identification of gametophytes and sporophytes, spores of the local species were cultured in vitro. In 18 months of trial, the appearance of gametophytes and sporophytes was observed, in a greater proportion those belonging to a dominant species in the community: Doryopteris concolor. Numerous gametophytes and sporophytes from Gastoniella chaerophylla were also obtained, a taxon not found in the “albardón” for two years. The germination index (GI) was estimated and the morphological characteristics of the gametophytes and sporophytes were recorded. This is the first contribution to the knowledge of the spore banks in Argentina, in a protected area where several threats put at risk the survival of native species. The bases to implement methods of ex situ and in situ conservation of native ferns are provided.

scholarly journals Leaf morphological and anatomical structure of pregenerative individuals of Ferula tadshikorum in ex situ conditions

2021 ◽  
Vol 7 ◽  
pp. 193-210
Author(s):  
Dilovar T. Khamraeva ◽  
Nina V. Grabovec ◽  
Rainer W Bussmann ◽  
Olim K. Khojimatov
Keyword(s):  
Raw Materials ◽  
Natural Populations ◽  
Botanical Garden ◽  
Anatomical Structure ◽  
Ex Situ ◽  
Vascular Bundles ◽  
Adaptive Responses ◽  
Anatomical Structures ◽  
Immature Development ◽  
Materials Research

For the first time, the morphological and anatomical structures of the leaves of different age individuals of the medicinal and rare species F. tadshikorum were studied in Tashkent Botanical Garden. Currently, natural populations of the species are in decline, and for the preservation and production of medicinal raw materials, research on the ex situ development conditions is needed. According to the results, adaptive responses to new habitat conditions in the anatomical structures of the vegetative organs were found. Plants of juvenile and immature development stages retained the the mesophyll's isolateral-palisade type, but differed in other quantitative and qualitative indicators of leaf tissues. Immature plants of the second year of life with more developed leaves showed modifications in the anatomical structure of the leaves, with powerfully developed vascular bundles, mechanical tissue, secretory ducts, as well as the presence of deep fibers both above the phloem of peripheral bundles, and also above the phloem of the central bundles of the petiole.

scholarly journals Morphological and Histo-Anatomical Study of Bryonia alba L. (Cucurbitaceae)

2015 ◽  
Vol 43 (1) ◽  
pp. 47-52
Author(s):  
Lavinia M. RUS ◽  
Irina I. IELCIU ◽  
Ramona PĂLTINEAN ◽  
Laurian VLASE ◽  
Cristina ŞTEFĂNESCU ◽  
...  
Keyword(s):  
Male Flower ◽  
Anatomical Study ◽  
Reproductive Organs ◽  
Sem Analysis ◽  
Anatomical Structure ◽  
Vascular Bundles ◽  
Anatomical Structures ◽  
Additional Information ◽  
Aerial Parts ◽  
First Time

The purpose of this study consisted in the identification of the macroscopic and microscopic characters of the vegetative and reproductive organs of Bryonia alba L., by the analysis of vegetal material, both integral and as powder. Optical microscopy was used to reveal the anatomical structure of the vegetative (root, stem, tendrils, leaves) and reproductive (ovary, male flower petals) organs. Histo-anatomical details were highlighted by coloration with an original combination of reagents for the double coloration of cellulose and lignin. Scanning electronic microscopy (SEM) and stereomicroscopy led to the elucidation of the structure of tector and secretory trichomes on the inferior epidermis of the leaf. Micrographic analysis of the powder obtained from aerial parts revealed segments of each organ (e.g. stomata, trichomes) and confirmed furthermore the results obtained by the histo-anatomical studies. Sections achieved through vegetative organs reveal typical anatomical structures: a transition to the secondary structure for the root, a typical dicotyledons’ structure with bicollateral vascular bundles for the stem, a bifacial structure of the leaf and stem-like structures for the tendrils and petioles, which prove they are metamorphosis of the stem. Anatomical structure of reproductive organs was performed hereby for the first time and revealed a typical anatomical structure for the 3-lodged ovary and a leaf-like structure of the male flower petal. Some of the results obtained confirm existing data from the scientific literature and additional information have been provided, outlining features that were not previously reported, such as SEM analysis of the leaf trichomes and histo-anatomical structure of the reproductive organs.

Ex Situ Conservation of Rare Medicinal Plants in the Botanical Garden (Institute) of Academy of Sciences of Moldova

2013 ◽  
Vol 40 (1) ◽  
pp. 43-48
Author(s):  
Nina Ciocârlan
Keyword(s):  
Native Species ◽  
Ex Situ Conservation ◽  
Botanical Garden ◽  
Ex Situ ◽  
Aromatic Plants ◽  
Medicinal And Aromatic Plants ◽  
Field Collection ◽  
Ecological Flexibility ◽  
Cultivation Techniques ◽  
Academy Of Sciences

Abstract This work refers to the native species of genus Astragalus L. (A. dasyanthus, A. ponticus), Adonis L. (A. vernalis, A. wolgensis) and Digitalis L. (D. lanata, D. grandiflora). The plants are cultivated in the Botanical Garden of Moldova in the field collection of the medicinal and aromatic plants. Investigation includes propagation aspects, research into cultivation techniques and conservation measures. The biological particularities and the phenologic rhythm are also registered. The obtained data shows the ecological flexibility of species and the possibility of preserving them in culture.

Investigation of 18 physiologically dormant Australian native species: germination response, environmental correlations and the implications for conservation

2020 ◽  
pp. 1-9
Author(s):  
Justin C. Collette ◽  
Mark K.J. Ooi
Keyword(s):  
Native Species ◽  
Strong Dependence ◽  
Ex Situ ◽  
Source Population ◽  
Seasonal Temperature ◽  
Environmental Correlations ◽  
Rainfall Season ◽  
Germination Response ◽  
Rainfall Seasonality ◽  
Germination Experiments

Abstract For physiologically dormant (PD) species in fire-prone environments, dormancy can be both complex due to the interaction between fire and seasonal cues, and extremely deep due to long intervals between recruitment events. Due to this complexity, there are knowledge gaps particularly surrounding the dormancy depth and cues of long-lived perennial PD species. This can be problematic for both in situ and ex situ species management. We used germination experiments that tested seasonal temperature, smoke, dark and heat for 18 PD shrub species distributed across temperate fire-prone Australia and assessed how germination was correlated with environmental factors associated with their home environments. We found extremely high levels of dormancy, with only eight species germinating above 10% and three species producing no germination at all. Seven of these eight species had quite specific seasonal temperature requirements and/or very strong responses to smoke cues. The maximum germination for each species was positively correlated with the mean temperature of the source population but negatively correlated with rainfall seasonality and driest months. The strong dependence on a smoke cue for some of the study species, along with examples from other studies, provides evidence that an obligate smoke response could be a fire-adapted germination cue. Germination response correlated with rainfall season of the source populations is a pattern which has often been assumed but little comparative data across sites with different rainfall seasonality exists. Further investigation of a broader range of species from different rainfall season environments would help to elucidate this knowledge gap.

scholarly journals An Anatomical Study of Scilla (Scilloideae) Section Chionodoxa and Scilla bifolia in Turkey1

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
H. YILDIRIM ◽  
K. YETISEN ◽  
A. ÖZDEMIR ◽  
C. ÖZDEMIR
Keyword(s):  
Numerical Analysis ◽  
Numerical Methods ◽  
Analysis Of Variance ◽  
Leaf Anatomy ◽  
Pearson Correlation ◽  
Anatomical Study ◽  
Anatomical Variations ◽  
Vascular Bundles ◽  
Single Row ◽  
Scilla Bifolia

ABSTRACT In the present study Scilla luciliae, S. forbesii, S. sardensis, S. siehei, Scilla x allenii and S. bifolia are compared anatomically. Some differences have been found in root, scape, and leaf anatomy of the taxa, and commented. S. luciliae, S. forbesii, S. sardensis and S. siehei have a metaxylem at the center of the root, others have 3-4 number metaxylem. Vascular bundles in two row in S. luciliae and S. forbesii in a single row in S. sardensis, Scilla x allenii and S. bifolia though in three rows in S. siehei. Aerenchyma tissue is present in mesophyll of five taxa leaf except S. sardensis. The anatomical variations in the taxa have been investigated by means of numerical methods (Analysis of variance and Pearson correlation). By the analysis of the investigated taxa from 12 anatomy related characters, it has been also found that the results from numerical analysis of anatomy characters can provide additional evidences, which correspond to the anatomy for the recognition of the taxa.

scholarly journals Adaptability and leaf anatomical features in oil palm seedlings produced by embryo rescue and pre-germinated seeds

2010 ◽  
Vol 22 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Zanderluce G. Luis ◽  
Kadja Milena G. Bezerra ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Oil Palm ◽  
Embryo Rescue ◽  
Morphological Characteristics ◽  
Growth Conditions ◽  
Epidermal Cells ◽  
In Vitro Cultivation ◽  
Vascular Bundles ◽  
Anatomical Plasticity ◽  
Germinated Seeds

Changes in the leaf structure of plants grown in different conditions have been reported, such as increase in size and density of stomata and reduction in stomatal control, amount of epicuticular wax, and mesophyll thickness, with a high diversity of intercellular spaces. However, these changes are highly variable depending on the physiological and morphological characteristics of each species. The objective of this work was to analyze the adaptability and anatomical plasticity of oil palm seedlings produced after embryo rescue and pre-germinated seeds. Expanded leaves were prepared for evaluation of morphometric data and anatomical structures. It was verified that the environmental conditions in vitro negatively influenced the stomata density, epidermal and hypodermal thickness, and the values for the expansion cells and leaf mesophile. Anatomically, the oil palm leaves present the same tissues composition in both growth conditions, with uniseriate epidermal cells, and tetracitic stomata occurring in both epidermal surfaces. Epidermal cells from in vitro plants are thinner than ones from greenhouse. The midrib of leaves from greenhouse plants are more developed and is composed by only one central vascular bundle, while plants from in vitro cultivation developed three to four collateral vascular bundles.

scholarly journals Leaf morphoanatomy of an endemic massaranduba from Chapada Diamantina, Bahia, Brazil

2021 ◽  
Vol 21 (3) ◽  
Author(s):  
Monick Lima Carvalho ◽  
Cláudia Elena Carneiro
Keyword(s):  
Stomatal Density ◽  
Vegetative State ◽  
Anatomical Study ◽  
Iucn Red List ◽  
Vascular Bundles ◽  
Leaf Architecture ◽  
Chapada Diamantina ◽  
Anatomical Features ◽  
The Family ◽  
Microscopy Techniques

Abstract: The Sapotaceae family is recognized for its economic importance, presenting food, medicinal and timber potential. Pouteria andarahiensis T.D.Penn., popularly known as "massaranduba", is endemic to Chapada Diamantina, Bahia, Brazil, and is currently classified on the IUCN red list as "endangered". Pouteria andarahiensis is little studied, highlighting this work as the first anatomical study for the species. Light microscopy and scanning electron microscopy techniques were used to perform anatomical studies. The species showed characters shared with the family (laticifers and malpiguiaceous trichomes), as well as diagnostic characters and associated with xeromorphy. The data obtained from the leaf architecture can assist in the identification of the species in a vegetative state, while the leaf surface provided unpublished data to the species, indicating the presence of a cuticle with complex ornamentation. Stand out as xeromorphic anatomical features, high stomatal density, high number of trichomes per area, sclerenchymatic columns in the mesophyll and a subepidermal sclerenchyma layer connecting the vascular bundles in the mesophyll.

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