Microlipid-Induced Oxidative Stress in Human Breastmilk: In Vitro Effects on Intestinal Epithelial Cells

2007 ◽  
Vol 2 (4) ◽  
pp. 209-218 ◽  
Author(s):  
William L. Diehl-Jones ◽  
Debra Fraser Askin ◽  
James K. Friel
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
In Vitro Effects

A thermal injury-induced circulating factor(s) compromises intestinal cell morphology, proliferation, and migration

1999 ◽  
Vol 277 (1) ◽  
pp. G175-G182 ◽  
Author(s):  
Maryam Varedi ◽  
George H. Greeley ◽  
David N. Herndon ◽  
Ella W. Englander
Keyword(s):  
Epithelial Cells ◽  
Thermal Injury ◽  
Intestinal Epithelial Cells ◽  
Intestinal Cell ◽  
Intestinal Epithelial ◽  
Rat Serum ◽  
In Vitro Effects ◽  
Mucosal Morphology ◽  
And Migration

The effects of a 60% body surface area thermal injury in rats on the morphology and proliferation of the epithelium of the small intestine and the in vitro effects of serum collected from scalded rats on intestinal epithelial cells were investigated. Scald injury caused significant reductions in duodenal villus width and crypt dimensions, villus enterocytes changed in shape from columnar to cuboidal, and the number of goblet cells decreased. The proportion of bromodeoxyuridine-labeled S phase cells in crypts was also diminished. In vitro, incubation of intestinal epithelial cells (IEC-6) with scalded rat serum (SRS) collected at either 12 or 24 h after injury caused a disruption in the integrity of the confluent culture and induced the appearance of large denuded areas. SRS also decreased DNA synthesis and delayed wound closure in an in vitro wound-healing model. The thermal injury-induced changes in intestinal mucosal morphology and epithelial cell growth characteristics described in this study may underlie, in part, the mechanism(s) involved in the diminished absorption of nutrients, increased intestinal permeability, and sepsis in patients with thermal injury.

Characterization of in vitro effects of microcystin-LR on intestinal epithelial cells

2016 ◽  
Vol 32 (5) ◽  
pp. 1539-1547 ◽  
Author(s):  
Yuan Zhou ◽  
Xiaoping Xu ◽  
Beibei Yu ◽  
Guang Yu
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
In Vitro Effects

Protective effect of glutathione against oxidative injury in intestinal epithelial cells of piglet in vitro

2009 ◽  
Vol 59 (3) ◽  
pp. 263-272 ◽  
Author(s):  
Qun Chen ◽  
Ying-Qiu Li ◽  
Shu-Ming Zhang ◽  
Hai-Yan Liu
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Oxidative Injury ◽  
Control Group ◽  
Dependent Manner ◽  
Intestinal Epithelial ◽  
High Concentration ◽  
Low Concentration

AbstractOxidative stress of intestinal epithelium is involved in inflammatory bowel disease. To investigate protective effects of glutathione (GSH) on xanthine/xanthine oxidase (X/XO)-induced oxidative injury in intestinal epithelial cells (IECs). We employed in vitro cell culture supplemented with X/XO. IECs were cultured for 72 h, and then divided into seven groups with various concentrations of X/XO and GSH supplementation in the medium. Agarose gel electrophoresis lanes indicated that X/XO induced DNA injury by the high concentration of XO (40, 70 U/L)-treated groups. The X/XO supplementation significantly increased the production of malondialdehyde (MDA) in a dose-dependent manner. There was a slight increase in total radical-trapping antioxidant potential (TRAP) value by the low concentration of XO (10U/L) alone-treated group (P > 0.05) while supplementation of a high concentration of XO (40, 70 U/L) significantly decreased TRAP value compared with XO (10 U/L) and the control group (P < 0.05). Addition of GSH decreased the production of MDA and DNA fragmentations (P < 0.05), but enhanced TRAP value (P < 0.05). These results suggest that IECs of piglet have the ability of enduring mild oxidative stress induced by a low concentration of XO. Although high concentrations of XO resulted in oxidation injury and lipid peroxidation in the IECs, additions of GSH to the medium showed significant protection against the X/XO-induced oxidative stress.

scholarly journals Conditional (intestinal-specific) knockout of the riboflavin transporter-3 (RFVT-3) impairs riboflavin absorption

2016 ◽  
Vol 310 (4) ◽  
pp. G285-G293 ◽  
Author(s):  
Veedamali S. Subramanian ◽  
Nils Lambrecht ◽  
Christian Lytle ◽  
Hamid M. Said
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Cell Metabolism ◽  
Protein Product ◽  
Intestinal Epithelial ◽  
Relative Contribution ◽  
Stress Responsive Genes

Riboflavin (RF) is indispensable for normal cell metabolism, proliferation, and growth. The RFVT-3 protein (product of the Slc52a3 gene) is expressed in the gut with the expression being restricted to the apical membrane domain of the polarized intestinal epithelial cells. The relative contribution of RFVT-3 to total carrier-mediated RF uptake in the native intestine, however, is not clear. We addressed this issue in the current investigation using a conditional (intestinal-specific) RFVT-3 knockout (cKO) mouse model developed by the Cre/Lox approach. All RFVT-3 cKO mice were found to be RF deficient and showed a significant growth and development retardation; also, nearly two-thirds of them died prematurely between the age of 6 and 12 wk. In vivo (intestinal and colonic loops) and in vitro (native isolated intestinal epithelial cells) uptake studies showed a severe inhibition in carrier-mediated RF uptake in the cKO mice compared with control littermates. We also observed a significant increase in the level of expression of oxidative stress-responsive genes in the intestine of the cKO mice compared with control littermates. Supplementation of the RFVT-3 cKO mice with pharmacological doses of RF led to a complete correction of the growth retardation and to normalization in the level of expression of the oxidative stress-responsive genes in the gut. These results show, for the first time, that the RFVT-3 system is the main transporter involved in carrier-mediated RF uptake in the native mouse small and large intestine, and that its dysfunction impairs normal RF body homeostasis.

Diospyros kaki extract protects myoblasts from oxidative stress-induced cytotoxicity via secretions derived from intestinal epithelium

2020 ◽  
Vol 85 (2) ◽  
pp. 430-439
Author(s):  
Nayla Majeda Alfarafisa ◽  
Kohji Kitaguchi ◽  
Tomio Yabe
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
C2c12 Cell ◽  
Diospyros Kaki ◽  
Intestinal Epithelial ◽  
Age Related ◽  
Loss Of Mass ◽  
Myoblast Cells

ABSTRACT Under oxidative stress, reactive oxygen species (ROS) alter signal transduction and induce macromolecular damage in cells. Such oxidative damage can lead to sarcopenia, an age-related syndrome characterized by a progressive loss of mass and strength of skeletal muscles. Because food components do not directly come in contact with muscle cells, we focused on the effects of secretions produced by stimulated intestinal epithelial cells on oxidative stress in myoblast cells. An extract of Diospyros kaki was fractionated using different concentrations of ethanol. Each fraction showed different levels of antioxidant and phenolic compounds. The biological activity was evaluated using a Caco-2 cell coculture system. Secretions from Caco-2 cells exposed to 0.5 mg/mL D. kaki extract attenuated the oxidative stress-induced reduction of C2C12 cell viability, suggesting that the D. kaki extract could stimulate intestinal epithelial cells to produce secretions that reduce oxidative stress in myoblasts in vitro.

scholarly journals Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 328 ◽  
Author(s):  
Claudio Salaris ◽  
Melania Scarpa ◽  
Marina Elli ◽  
Alice Bertolini ◽  
Simone Guglielmetti ◽  
...  
Keyword(s):  
Immune Response ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Plaque Assay ◽  
Immune Response Gene ◽  
Intestinal Epithelial ◽  
Antiviral Immune Response ◽  
Qrt Pcr ◽  
Anti Inflammatory

SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection.

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