Studies on Silver Nanoparticle–Glycyrrhizic Acid Complex as a Radioprotector and an Adjuvant in Radiotherapy Under In Vivo Conditions

2012 ◽  
Vol 27 (10) ◽  
pp. 642-651 ◽  
Author(s):  
Dhanya K. Chandrasekharan ◽  
Cherupally Krishnan Krishnan Nair
Keyword(s):  
Silver Nanoparticle ◽  
Glycyrrhizic Acid ◽  
Acid Complex

scholarly journals Interaction of Lactoferrin with Unsaturated Fatty Acids: In Vitro and In Vivo Study of Human Lactoferrin/Oleic Acid Complex Cytotoxicity

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1602
Author(s):  
Anna Elizarova ◽  
Alexey Sokolov ◽  
Valeria Kostevich ◽  
Ekaterina Kisseleva ◽  
Evgeny Zelenskiy ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Structural Changes ◽  
Unsaturated Fatty Acids ◽  
Structural Features ◽  
Control Group ◽  
Acid Complex ◽  
Hepatoma 22A ◽  
Constitutive Parameters

As shown recently, oleic acid (OA) in complex with lactoferrin (LF) causes the death of cancer cells, but no mechanism(s) of that toxicity have been disclosed. In this study, constitutive parameters of the antitumor effect of LF/OA complex were explored. Complex LF/OA was prepared by titrating recombinant human LF with OA. Spectral analysis was used to assess possible structural changes of LF within its complex with OA. Structural features of apo-LF did not change within the complex LF:OA = 1:8, which was toxic for hepatoma 22a cells. Cytotoxicity of the complex LF:OA = 1:8 was tested in cultured hepatoma 22a cells and in fresh erythrocytes. Its anticancer activity was tested in mice carrying hepatoma 22a. In mice injected daily with LF-8OA, the same tumor grew significantly slower. In 20% of animals, the tumors completely resolved. LF alone was less efficient, i.e., the tumor growth index was 0.14 for LF-8OA and 0.63 for LF as compared with 1.0 in the control animals. The results of testing from 48 days after the tumor inoculation showed that the survival rate among LF-8OA-treated animals was 70%, contrary to 0% rate in the control group and among the LF-treated mice. Our data allow us to regard the complex of LF and OA as a promising tool for cancer treatment.

scholarly journals In vivo Genotoxicity of Silver Nanoparticles after 90-day Silver Nanoparticle Inhalation Exposure

2011 ◽  
Vol 2 (1) ◽  
pp. 34-38 ◽  
Author(s):  
Jin Sik Kim ◽  
Jae Hyuck Sung ◽  
Jun Ho Ji ◽  
Kyung Seuk Song ◽  
Ji Hyun Lee ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Inhalation Exposure

TOXICITY STUDIES ON SILVER AND COPPER NANOPARTICLES

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (09) ◽  
pp. 58-60
Author(s):  
P Samuel ◽  
◽  
B Pavithra ◽  
R. Priyadarshini ◽  
V. Maheswari ◽  
...  
Keyword(s):  
Atomic Absorption ◽  
Copper Nanoparticles ◽  
Silver Nanoparticle ◽  
Danio Rerio ◽  
Atomic Absorption Spectroscopy ◽  
Pilot Scale ◽  
Zebra Fish ◽  
Ventral Region ◽  
Plant Sources

In a pilot scale study, silver and copper nanoparticles were synthesized from two different plant sources viz Flacourtia indica and Prosopsis juliflora. The in vivo toxicity of silver and copper nanoparticles was tested on Danio rerio (Zebra fish) under different concentrations (1 ppm, 10 ppm and 100 ppm). Through the investigation, the nanoparticles treated fishes developed with hyper pigmentation in the ventral region. The minimum lethal concentration required to bring lethality caused by silver nanoparticle was 10 ppm whereas for copper nanoparticles it was 1 ppm. Further, the concentration of silver and copper nanoparticles accumulated inside the fish was evaluated by Atomic Absorption Spectroscopy. The in vivo concentration of silver and copper nanoparticles steadily increases with increase in dosage of nanoparticles being tested.

Formulation of Chitosan Stabilized Silver Nanoparticle-Containing Wound Healing Film: In Vitro and In Vivo Characterization

2020 ◽  
Vol 109 (7) ◽  
pp. 2196-2205 ◽  
Author(s):  
Chintan Pansara ◽  
Renuka Mishra ◽  
Tejal Mehta ◽  
Ankit Parikh ◽  
Sanjay Garg
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticle ◽  
In Vivo Characterization

SYNTHESIS OF IODINE-CONTAINING NANO-DISPERSED COMPOSITES

2016 ◽  
Vol 1 (4) ◽  
pp. 79-82
Author(s):  
Григорьев ◽  
Vasiliy Grigorev ◽  
Козлов ◽  
Valeriy Kozlov ◽  
Мамцев ◽  
...  
Keyword(s):  
High Performance ◽  
Glycyrrhizic Acid ◽  
Organic Matrix ◽  
Advanced Technology ◽  
Human Organism ◽  
Organic Mineral ◽  
Inorganic Iodine ◽  
Physical And Chemical ◽  
Iodobenzoic Acid

The purpose of research is to develop techniques for chemical implementation of the obtained low molecular weight products with covalently bound iodine in the biodegradable matrix (glycyrrhizic acid) with obtaining nanosized composites, capable of metered cleavage of iodine in vivo. One of the vital trace elements is iodine. Adding iodine to food components is an advanced technology and it promotes replenishment of iodine deficiency in human organism. The most interesting compounds wherethe inorganic iodine are chemically bound to the organic matrix. The processes of formation of the complex "o-iodobenzoic acid-β-glycyrrhizic acid" and encapsulation mechanisms of 5-iodo-6-metiluratsila in nanoscale biodegradable matrix -β-glycyrrhizic acid by using physical and chemical methods of analysis – IR, UV and NMR spectroscopy. The evaluationof nano-sized ofsynthesized iodinebioorganic complexes by laser nanostructured analysis is carried out. The processes of deiodination o-iodobenzoic acid in vivo by high-performance liquid chromatography is examed. The conducted research showsthe bioavailability of synthesized nanostructured iodine organic-mineral complex.

scholarly journals In Situ Silver-based Electrochemical Bioreactor In Vivo

2020 ◽  
Author(s):  
Yong Huang ◽  
Liping Zhong ◽  
Zhiming Deng ◽  
Pan Wu ◽  
Jian He ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Graphene Oxide ◽  
Tumor Cells ◽  
Silver Nanoparticle ◽  
A549 Cells ◽  
Induced Apoptosis ◽  
Induced Changes

Abstract In this study we show for the first time that a reduced graphene oxide (rGO) carrier has a 15-fold higher catalysis rate than graphene oxide (GO) in Ag+ reduction. Based on this, we constructed a tumor microenvironment-enabled in situ silver-based electrochemical oncolytic bioreactor (SEOB) which unlocked an Ag+ prodrug to generate silver nanoparticles and inhibited the growth of various tumors. In this bioreactor system, intratumoral H2O2 acted as the reductant and the rGO carrier acted as the catalyst. Chelation of aptamers to this prodrug increased the production of silver nanoparticles by tumor cells, especially in the presence of Vitamin C, which broke down in tumor cells to supply massive amounts of H2O2. Consequently, highly efficient silver nanoparticle-induced apoptosis was observed in HepG2 and A549 cells in vitro and in HepG2- and A549-derived tumors in vivo. The apoptosis was associated with ROS-induced changes in mitochondrial membrane potential and DNA damage. The specific aptamer targeting and intratumoral silver nanoparticle production guaranteed excellent biosafety, with no damage to normal cells, because the Ag+ prodrug was specifically unlocked in tumors. More significantly, there was no evident tissue damage in monkeys, which greatly increases the clinical translation potential of the SEOB system.

scholarly journals In vivo biocompatibility of new nano-calcium-deficient hydroxyapatite/poly-amino acid complex biomaterials

2015 ◽  
pp. 6303 ◽  
Author(s):  
Zhenyu Dai ◽  
Yue Li ◽  
Weizhong Lu ◽  
Dian-ming Jiang ◽  
Hua Hong ◽  
...  
Keyword(s):  
Amino Acid ◽  
Acid Complex ◽  
Amino Acid Complex

Silver nanoparticle/bacterial cellulose gel membranes for antibacterial wound dressing: investigation in vitro and in vivo

2014 ◽  
Vol 9 (3) ◽  
pp. 035005 ◽  
Author(s):  
Jian Wu ◽  
Yudong Zheng ◽  
Xiaoxiao Wen ◽  
Qinghua Lin ◽  
Xiaohua Chen ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Silver Nanoparticle ◽  
Wound Dressing ◽  
Gel Membranes ◽  
Cellulose Gel

Glycyrrhizic Acid Modulates Apoptosis through Extrinsic/Intrinsic Pathways and Inhibits Protein Kinase B- and Extracellular Signal-Regulated Kinase-Mediated Metastatic Potential in Hepatocellular Carcinoma In Vitro and In Vivo

2020 ◽  
Vol 48 (01) ◽  
pp. 223-244
Author(s):  
Jai-Jen Tsai ◽  
Po-Jung Pan ◽  
Fei-Ting Hsu ◽  
Jing-Gung Chung ◽  
I-Tsang Chiang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Invasion ◽  
Glycyrrhizic Acid ◽  
Metastatic Potential ◽  
Signaling Transduction ◽  
Apoptotic Signaling ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal

A previous study presented that glycyrrhizic acid as the hepatoprotective agent inhibits total parenteral nutrition-associated acute liver injury in rats. However, the anticancer effect and mechanism of glycyrrhizic acid in human hepatocellular carcinoma (HCC) is ambiguous. The purpose of the present study was to investigate the effect of glycyrrhizic acid on apoptosis dysregulation and metastatic potential in HCC in vitro and in vivo. Both SK-Hep1 and Hep3B cells were treated with different concentrations of glycyrrhizic acid for 24 or 48[Formula: see text]h. SK-Hep1/luc2 tumor-bearing mice were treated with vehicle or glycyrrhizic acid (50[Formula: see text]mg/kg/day by intraperitoneal injection) for 7 days. Tumor cells growth, apoptotic, and metastatic signaling transduction were evaluated by using MTT assay, digital caliper, bioluminescence imaging (BLI), flow cytometry, western blotting assay, and immunohistochemistry (IHC) staining. The results demonstrated glycyrrhizic acid significantly inhibits tumor cell growth, cell invasion, and expression of AKT (Ser473), extracellular-signal-regulated kinase (ERK), epidermal growth factor receptor (EGFR) phosphorylation, anti-apoptotic and metastatic proteins in HCC in vitro and in vivo. Glycyrrhizic acid also significantly triggered apoptosis and extrinsic/intrinsic apoptotic signaling transduction. In addition, PD98059 (ERK inhibitor) and LY294002 (AKT inhibitor) obviously reduced cell invasion and expression of metastasis-associated proteins. Taken together, these results indicated that glycyrrhizic acid induces apoptosis through extrinsic/intrinsic apoptotic signaling pathways and diminishes EGFR/AKT/ERK-modulated metastatic potential in HCC in vitro and in vivo.

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