Antimicrobial Resistance in Coagulase-Positive Staphylococci Isolated from Various Animals in Bosnia and Herzegovina

2021 ◽  
Author(s):  
Zinka Maksimović ◽  
Jasmin Dizdarević ◽  
Senka Babić ◽  
Maid Rifatbegović
Keyword(s):  
Antimicrobial Resistance ◽  
Bosnia And Herzegovina ◽  
Coagulase Positive Staphylococci

scholarly journals Nasal carriage rate and antimicrobial resistance pattern of Staphylococcus aureus among the food handlers in Canton Sarajevo, Bosnia and Herzegovina

2020 ◽  
Author(s):  
Sabina Šegalo ◽  
Daniel Maestro ◽  
Zarema Obradović ◽  
Anes Jogunčić
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Bosnia And Herzegovina ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Nasal Carriage ◽  
Antimicrobial Susceptibility Testing ◽  
Food Handlers ◽  
Carriage Rate

Introduction: The nasals and hand carriage of Staphylococcus aureus in food handlers (FHs) represent a significant source of Staphylococcal food contamination and food poisoning. Antimicrobial resistance (AMR) is a microorganism’s ability to resist the action of one or more antimicrobial agents. S. aureus has demonstrated the ability to rapidly respond to each new antimicrobial with the development of a resistance mechanism. The aim of the study was to assess the prevalence of nasal carriage rate and AMR pattern of isolated strains S. aureus among FHs in Canton Sarajevo, Bosnia and Herzegovina. Methods: The retrospective study included laboratory results of 11.139 tested subjects between January 2014 and December 2018. The study was conducted in the laboratory of the Institute of Public Health of the Federation of Bosnia and Herzegovina in Sarajevo. Samples of nasal swabs were collected from FHs, employees in companies located in Canton Sarajevo, during sanitary surveillance prescribed by applicable legal standards. S. aureus isolates were identified according to conventional microbiological methods and antimicrobial susceptibility testing was performed by the agar disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing; 2013 standard. Results: Among the 11.138 subjects, 792 (7.1%) were carriers of S. aureus. Isolated strains were tested on eight different antibiotics, and the resistance to penicillin, ampicillin, and amoxicillin was 788 (99.5%), 776 (97.9%), and 752 (94.9%), retrospectively. In total, 86.36% of isolated strains were multidrug-resistant. Conclusions: The low percentage of S. aureus carriers indicates that preventive measures of carrier control are being actively implemented within the legally prescribed measures. The emergence of numerous isolated strains with multidrug-resistance characteristics is a significant public health problem and consequently limits the range of antibiotics available for therapeutic purposes. The results of this research indicate that AMR has increased in Sarajevo Canton and it is following the trend of global growth.

scholarly journals Genes on the Move: In Vitro Transduction of Antimicrobial Resistance Genes between Human and Canine Staphylococcal Pathogens

2020 ◽  
Vol 8 (12) ◽  
pp. 2031
Author(s):  
Sian Marie Frosini ◽  
Ross Bond ◽  
Alex J. McCarthy ◽  
Claudia Feudi ◽  
Stefan Schwarz ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
One Health ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Tetracycline Resistance ◽  
Methicillin Resistant ◽  
Antimicrobial Resistance Genes ◽  
Resistance Transfer ◽  
Coagulase Positive Staphylococci

Transmission of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) between people and pets, and their co-carriage, are well-described. Potential exchange of antimicrobial resistance (AMR) genes amongst these staphylococci was investigated in vitro through endogenous bacteriophage-mediated transduction. Bacteriophages were UV-induced from seven donor isolates of canine (MRSP) and human (MRSA) origin, containing tet(M), tet(K), fusB or fusC, and lysates filtered. Twenty-seven tetracycline- and fusidic acid- (FA-) susceptible recipients were used in 122 donor-recipient combinations (22 tetracycline, 100 FA) across 415 assays (115 tetracycline, 300 FA). Bacteriophage lysates were incubated with recipients and presumed transductants quantified on antimicrobial-supplemented agar plates. Tetracycline resistance transduction from MRSP and MRSA to methicillin-susceptible S. pseudintermedius (MSSP) was confirmed by PCR in 15/115 assays. No FA-resistance transfer occurred, confirmed by negative fusB/fusC PCR, but colonies resulting from FA assays had high MICs (≥32 mg/L) and showed mutations in fusA, two at a novel position (F88L), nine at H457[Y/N/L]. Horizontal gene transfer of tetracycline-resistance confirms that resistance genes can be shared between coagulase-positive staphylococci from different hosts. Cross-species AMR transmission highlights the importance of good antimicrobial stewardship across humans and veterinary species to support One Health.

Prevalence and antimicrobial resistance of coagulase-positive staphylococci isolated from raw goat milk

2015 ◽  
Vol 123 (1) ◽  
pp. 124-128 ◽  
Author(s):  
Jolanta G. Rola ◽  
Maciej Sosnowski ◽  
Monika Ostrowska ◽  
Jacek Osek
Keyword(s):  
Antimicrobial Resistance ◽  
Goat Milk ◽  
Coagulase Positive Staphylococci

scholarly journals Artisan cheese as vehicle of contamination Escherichia coli and coagulase-positive staphylococci antimicrobial resistance

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Norma Suely Evangelista-Barreto ◽  
Gleyde Córdova da França Santos ◽  
Jerfeson dos Santos Souza ◽  
Fernanda de Sousa Bernardes ◽  
Irana Paim Silva
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Coagulase Positive Staphylococci

Antimicrobial resistance in bacteria isolated from pigeons in Poland

2014 ◽  
Vol 17 (1) ◽  
pp. 169-171 ◽  
Author(s):  
T. Stenzel ◽  
A. Bancerz-Kisiel ◽  
B. Tykałowski ◽  
M. Śmiałek ◽  
D. Pestka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
E Coli ◽  
Coagulase Positive Staphylococci

Abstract The present study investigated the drug-resistance to the selected antibiotics in Escherichia coli, Salmonella typhimurium and beta-haemolytic coagulase-positive staphylococci isolated from pigeons bred in Poland. In the case of E. coli, tetracyclines and amoxicillin were least effective. In the staphylococci, the highest resistance was detected for oxytetracycline and quinolones and 5% were resistant to methicillin. The lowest drug-resistance was reported for Salmonella typhimurium.

scholarly journals Antimicrobial resistance patterns of bacteria isolated from dogs with otitis

2019 ◽  
Vol 147 ◽  
Author(s):  
C. Bourély ◽  
G. Cazeau ◽  
N. Jarrige ◽  
A. Leblond ◽  
J.Y. Madec ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Close Contact ◽  
Control Strategies ◽  
Acquired Resistance ◽  
Additive Models ◽  
Resistant Bacteria ◽  
Surveillance Network ◽  
Resistance Patterns ◽  
Causative Agents ◽  
Coagulase Positive Staphylococci

AbstractAntimicrobial-resistant bacteria in dogs can be transmitted to humans and close contact between dogs and people might foster dissemination of resistance determinants. The aim of our study was to describe the antimicrobial resistance (AMR) pattern of the major causative agents of canine otitis – one of the most common diseases in dogs – isolated in France. Data collected between 2012 and 2016 by the French national surveillance network for AMR, referred to as RESAPATH, were analysed. Resistance trends were investigated using non-linear analysis (generalised additive models). A total of 7021 antibiograms were analysed. The four major causative agents of canine otitis in France were coagulase-positive staphylococci, Pseudomonas aeruginosa, Proteus mirabilis and streptococci. Since 2013, resistance to fluoroquinolones has been on the decrease in both P. aeruginosa and Staphylococcus pseudintermedius isolates. For P. aeruginosa, 19.4% of isolates were resistant to both enrofloxacin and gentamicin. The levels of multidrug resistance (acquired resistance to at least one antibiotic in three or more antibiotic classes) ranged between 11.9% for P. mirabilis and 16.0% for S. pseudintermedius. These results are essential to guide prudent use of antibiotics in veterinary medicine. They will also help in designing efficient control strategies and in measuring their effectiveness.

scholarly journals Antimicrobial resistance in coagulase-positive staphylococci isolated from companion animals in Australia: A one year study

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0176379 ◽  
Author(s):  
Sugiyono Saputra ◽  
David Jordan ◽  
Kate A. Worthing ◽  
Jacqueline M. Norris ◽  
Hui S. Wong ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Companion Animals ◽  
One Year ◽  
Coagulase Positive Staphylococci

scholarly journals Antimicrobial Resistance Profile and Biofilm Production of Microorganisms Isolated from Oropharynx of Rupornis magnirostris (Gmelin, 1788) and Caracara plancus (Miller, 1777)

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Fernanda Alda da Silva ◽  
Sandrelli Meridiana de Fátima Ramos dos Santos Medeiros ◽  
Sérgio Dias da Costa-Junior ◽  
Ana Emília Medeiros Roberto ◽  
Sarah Brandão Palácio ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Rhodotorula Mucilaginosa ◽  
Urban Centers ◽  
Resistance Profile ◽  
Biofilm Production ◽  
Violet Crystal ◽  
Antimicrobial Resistance Profile ◽  
Staining Methods ◽  
Preliminary Study ◽  
Coagulase Positive Staphylococci

The aim of this preliminary study was to identify microorganisms with antimicrobial resistance profile and biofilm producers in oropharynx of Rupornis magnirostris and Caracara plancus. Six R. magnirostris and six C. plancus maintained in Triage Center for Wild Animals (CETAS) facilities were studied. Coagulase-positive staphylococci (CoPS), enterobacteria, and yeasts were identified by the biochemical analysis or MALDI-TOF mass spectrometry. The resistance profile of the microorganisms was analyzed according to CLSI. The biofilm production was evaluated by Congo red and violet crystal staining methods. Among the 12 birds, 10 presented strains of CoPS and/or enterobacteria with resistance profile, such as methicillin-resistant CoPS (MR-CoPS), vancomycin-resistant CoPS (VR-CoPS), extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL), and Klebsiella pneumoniae carbapenemase- (KPC-) producing bacteria. Regards the fungal analysis, Candida spp., Cryptococcus spp., Rhodotorula mucilaginosa, R. glutinis, and Trichosporon coremiiforme were identified. All the Trichosporon coremiiforme strains were resistant to amphotericin B, as well as all the Rhodotorula mucilaginosa exhibited resistance to fluconazole. Related to the biofilm production, among the 8 CoPS, 27 enterobacteria, and 10 yeasts isolates, 3, 16, and 7 strains were biofilm producers, respectively. Thus, the presence of these microorganisms in birds of prey is worrisome, highlighting its possible influence in the spread of infections in urban centers.

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