Histological and TEM Examination of Early Stages of Bone Healing after Er:YAG Laser Irradiation

2004 ◽  
Vol 22 (4) ◽  
pp. 342-350 ◽  
Author(s):  
Amir Pourzarandian ◽  
Hisashi Watanabe ◽  
Akira Aoki ◽  
Shizuko Ichinose ◽  
Katia M. Sasaki ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Bone Healing ◽  
Er:Yag Laser ◽  
Early Stages

Platelet Derived Growth Factor Secretion and Bone Healing After Er:YAG Laser Bone Irradiation

2011 ◽  
Vol 37 (sp1) ◽  
pp. 195-204 ◽  
Author(s):  
Gavriel Kesler ◽  
Dana Kesler Shvero ◽  
Yariv Siman Tov ◽  
George Romanos
Keyword(s):  
Growth Factor ◽  
Laser Irradiation ◽  
Bone Healing ◽  
Er:Yag Laser ◽  
Test Group ◽  
Significant Rise ◽  
Platelet Derived Growth Factor ◽  
Control Group ◽  
Saline Irrigation ◽  
Post Surgery

Er:YAG laser irradiation has been reported to enhance wound healing. However, no studies have evaluated the synthesis of growth factors after laser irradiation. The present study investigated the effects of laser irradiation on the amount of secretion of platelet derived growth factor (PDGF) in the wound, clarifying the effects of the Er:YAG laser on the bone healing. Osteotomies were prepared in the tibiae of 28 rats using an Er:YAG laser (test group). Maximum power of 8 watts, energy per pulse of 700 mJ, and frequency up to 50 Hz were used. The laser was used with external water irrigation, a spot size of 2 mm, energy per pulse of 500 to 1000 mJ/pulse, and energy density of 32 J/cm2. Twenty eight additional rats served as a control group and their osteotomies were prepared with a drill 1.3 mm in diameter at 1000 rpm, with simultaneous saline irrigation. Two rats from the tested group and 2 from the control group were sacrificed on each day following surgery (1–14 days), and the tissue specimens were prepared for histologic evaluation. Immunohistochemical staining with anti-PDGF was performed after histologic examination. The difference between the PDGF staining intensities of the 2 treatment groups was analyzed using a multivariate logistic regression test. A significant rise in PDGF staining occurred in both groups 2–3 days following surgery. However, while high PDGF counts remained for the 2-week experimental period in the laser group, PDGF levels in the control group returned to baseline levels 8 days post surgery. The 2 groups (laser and control) were found to be different throughout the experiment, and the rat type was found to be a significant predictor (P  =  .000011). The present study demonstrated that Er:YAG laser irradiation seems to stimulate the secretion of PDGF in osteotomy sites in a rat model. It is possible that the high levels of PDGF are part of the mechanism that Er:YAG irradiation enhances and improves the healing of osteotomy sites.

Effects of the Er:YAG Laser Irradiation on Titanium Implant Materials and Contaminated Implant Abutment Surfaces

2003 ◽  
Vol 21 (1) ◽  
pp. 7-17 ◽  
Author(s):  
Tomoko Matsuyama ◽  
Akira Aoki ◽  
Shigeru Oda ◽  
Takayuki Yoneyama ◽  
Isao Ishikawa
Keyword(s):  
Laser Irradiation ◽  
Er:Yag Laser ◽  
Titanium Implant ◽  
Implant Materials ◽  
Implant Abutment

Surface temperature during cavity preparation on human tooth by Er:YAG laser irradiation

CIRP Annals ◽  
2011 ◽  
Vol 60 (1) ◽  
pp. 555-558 ◽  
Author(s):  
T. Furumoto ◽  
T. Ueda ◽  
A. Kasai ◽  
A. Hosokawa
Keyword(s):  
Surface Temperature ◽  
Laser Irradiation ◽  
Er:Yag Laser ◽  
Human Tooth ◽  
Cavity Preparation

scholarly journals Er:YAG laser-induced damage to a dental composite in simulated clinical scenarios for inadvertent irradiation: an in vitro study

2021 ◽  
Author(s):  
Katharina Kuhn ◽  
Carmen U. Schmid ◽  
Ralph G. Luthardt ◽  
Heike Rudolph ◽  
Rolf Diebolder
Keyword(s):  
Laser Irradiation ◽  
Laser Fluence ◽  
Ablation Threshold ◽  
Er:Yag Laser ◽  
In Vitro Study ◽  
Vitro Study ◽  
Crater Diameter ◽  
Clinical Scenarios ◽  
Laser Induced Damage

AbstractInadvertent Er:YAG laser irradiation occurs in dentistry and may harm restorative materials in teeth. The aim of this in vitro study was to quantify Er:YAG laser-induced damage to a nanohybrid composite in simulated clinical scenarios for inadvertent direct and indirect (reflection) laser irradiation. The simulation was performed by varying the output energy (OE;direct˃indirect) reaching the specimen and the operating distance (OD;direct˂indirect). Composite specimens were irradiated by an Er:YAG laser. The ablation threshold was determined and clinically relevant parameters were applied (n = 6 for each OE/OD combination) for direct (OE: 570 mJ/OD: 10 mm, OE: 190 mJ/OD: 10 mm) and indirect irradiation (OE: 466 mJ/OD: 15 mm, OE: 57 mJ/OD: 15 mm, OE: 155 mJ/OD: 15 mm, OE: 19 mJ/OD: 15 mm). The extent of damage in the form of craters was evaluated using a laser scanning microscope (LSM) and a conventional light microscope (LM). The ablation threshold was determined to be 2.6 J/cm2. The crater diameter showed the highest value (LM: 1075 ± 18 µm/LSM: 1082 ± 17 µm) for indirect irradiation (reflectant:dental mirror) (OE: 466 mJ/OD: 15 mm). The crater depth showed the highest and comparable value for direct (OE: 570 mJ/OD: 10 mm; LSM: 89 ± 2 µm) and indirect irradiation (OE: 466 mJ/OD: 15 mm; LSM: 90 ± 4 µm). For each OD, the crater diameter, depth, and volume increased with higher laser fluence. However, the OD—and thus the laser spot diameter—also had an enlarging effect. Thus, indirect irradiation (reflectant:dental mirror) with only 47% of the laser fluence of direct irradiation led to a larger diameter and a comparable depth. The three-dimensional extent of the crater was large enough to cause roughening, which may lead to plaque accumulation and encourage caries, gingivitis, and periodontitis under clinical conditions. Clinicians should be aware that reflected irradiation can still create such craters.

scholarly journals Human Dental Enamel and Dentin Structural Effects after Er:yag Laser Irradiation

2014 ◽  
Vol 15 (3) ◽  
pp. 283-287 ◽  
Author(s):  
Mateus Rodrigues Tonetto ◽  
Marcelo Ferrarezi de Andrade ◽  
Shelon Cristina Souza Pinto ◽  
Darlon Martins Lima ◽  
José Roberto Cury Saad ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Soft Tissues ◽  
Er:Yag Laser ◽  
Laser System ◽  
Dental Enamel ◽  
Target Tissue ◽  
Structural Effects ◽  
Ablation Process ◽  
Dental Tissues ◽  
Laser Systems

ABSTRACT Ideally projected to be applied on soft tissues, infrared lasers were improved by restorative dentistry to be used in hard dental tissues cavity preparations — namely enamel and dentin. This paper evidentiates the relevant aspects of infrared Erbium laser's action mechanism and its effects, and characterizes the different effects deriving from the laser's beams emission. The criteria for use and selection of optimal parameters for the correct application of laser systems and influence of supporting factors on the process, such as water amount and its presence in the ablation process, protection exerted by the plasma shielding and structural factors, which are indispensable in dental tissues cavity preparation related to restorative technique, are subordinated to optical modifications caused by the interaction of the energy dissipated by these laser light emission systems in the targeted tissue substrate. Clinical relevance Differences in the action of infrared Erbium laser system in regard to the nature of the ablation process and variations on the morphological aspects observed in the superficial structure of the target tissue irradiated, may be correlated to the structural optical modifications of the substrate produced by an interaction of the energy propagated by laser systems. How to cite this article Lima DM, Tonetto MR, de Mendonça AAM, Elossais AA, Saad JRC, de Andrade MF, Pinto SCS, Bandéca MC. Human Dental Enamel and Dentin Structural Effects after Er:yag Laser Irradiation. J Contemp Dent Pract 2014;15(3):283-287.

Effect of Er:YAG Laser Irradiation on Bonding Property of Zirconia Ceramics to Resin Cement

2013 ◽  
Vol 31 (12) ◽  
pp. 619-625 ◽  
Author(s):  
Yihua Lin ◽  
Xiaomeng Song ◽  
Yaming Chen ◽  
Qingping Zhu ◽  
Wei Zhang
Keyword(s):  
Laser Irradiation ◽  
Er:Yag Laser ◽  
Resin Cement ◽  
Zirconia Ceramics ◽  
Bonding Property

scholarly journals Integrating CAD and 3D-Printing Techniques to Construct an In Vitro Laser Standard Treatment Platform for Evaluating the Effectiveness of Sterilization by Er:YAG Laser in Peri-Implant Intra-Bony Defects

2020 ◽  
Vol 10 (10) ◽  
pp. 3431
Author(s):  
Shih-Hao Chang ◽  
Hsiang-I Mei ◽  
Chun-Li Lin
Keyword(s):  
Escherichia Coli ◽  
3D Printing ◽  
Laser Irradiation ◽  
Dental Implants ◽  
Bacterial Adhesion ◽  
Er:Yag Laser ◽  
Bony Defect ◽  
Mandibular Incisor ◽  
Printing Techniques

This study established an in vitro model mimicking clinical peri-implant intra-bony defects. We investigated the effect of access limitation and the bactericidal effectiveness of erbium-doped yttrium, aluminum and garnet (Er:YAG) laser irradiation in shallow and deep peri-implant defects at different tooth positions. Reverse engineering, computer-aided design (CAD), and 3D-printing techniques were integrated to establish physical peri-implant intra-bony defect models at mandibular central incisor, first premolar, and first molar positions with shallow (2 mm depth) or deep (6 mm depth) defects and with 1.5 mm and 1.8 mm widths at the bottom and crestal portions of the alveolar process, respectively. Three-dimensional printed suites at the corresponding implant sites replaced experimental implant specimens for the investigation of bacterial adhesion in individuals. Dental implants with diameters of 3, 4 and 5 mm were utilized at the mandibular incisor, premolar, and molar positions, respectively. Bacterial adhesion of Gram (–) Escherichia coli on the exposed implant surfaces prior to sterilization was assessed. Sterilization with shallow and deep intra-bony defects was investigated by measuring the reduction of residual viable bacteria on implants after 60 s of irradiation with an Er:YAG laser. The adhesion rate of Gram (–) Escherichia coli on the investigated implant surfaces ranged from 1% to 3% (1.76 ± 1.25%, 2.19   ±   0.75% and 2.66   ±   1.26% for 3, 4, and 5 mm implants, respectively). With shallow peri-implant bony defects, the Er:YAG laser sterilization rates were 99.6 ± 0.5%, 99.3   ±   0.41% and 93.8 ± 7.65% at mandibular incisor, premolar, and molar positions, respectively. Similarly, sterilization rates in deep peri-implant defects were 99 ± 1.35%, 99.1 ± 0.98% and 97.14 ± 2.57%, respectively. A 3D-printed model with replaceable implant specimens mimicking human peri-implant intra-bony defects was established and tested in vitro. This investigation demonstrated effective sterilization using Er:YAG laser irradiation in both shallow and deep peri-implant intra-bony defects at different positions and diameters of dental implants.

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