Molecular Characterization ofAnaplasmasp. in Free-Living Gray Brockets(Mazama gouazoubira)

2017 ◽  
Vol 17 (3) ◽  
pp. 165-171 ◽  
Author(s):  
Anna Claudia Baumel Mongruel ◽  
Jyan Lucas Benevenute ◽  
Marcos Rogério André ◽  
Adriano de Oliveira Torres Carrasco ◽  
Rosangela Zacarias Machado ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Free Living ◽  
Mazama Gouazoubira

scholarly journals Post-mortem hemoparasite detection in free-living Brazilian brown brocket deer (Mazama gouazoubira, Fischer 1814)

2014 ◽  
Vol 23 (2) ◽  
pp. 206-215 ◽  
Author(s):  
Júlia Angélica Gonçalves da Silveira ◽  
Élida Mara Leite Rabelo ◽  
Paula Cristina Senra Lima ◽  
Bárbara Neves Chaves ◽  
Múcio Flávio Barbosa Ribeiro
Keyword(s):  
Nested Pcr ◽  
Pcr Analysis ◽  
Post Mortem ◽  
Infectious Agents ◽  
Free Living ◽  
Molecular Assays ◽  
Minas Gerais State ◽  
Mazama Gouazoubira ◽  
Nested Pcr Assays ◽  
Brocket Deer

Tick-borne infections can result in serious health problems for wild ruminants, and some of these infectious agents can be considered zoonosis. The aim of the present study was the post-mortem detection of hemoparasites in free-living Mazama gouazoubira from Minas Gerais state, Brazil. The deer samples consisted of free-living M. gouazoubira (n = 9) individuals that died after capture. Necropsy examinations of the carcasses were performed to search for macroscopic alterations. Organ samples were collected for subsequent imprint slides, and nested PCR assays were performed to detect hemoparasite species. Imprint slide assays from four deer showed erythrocytes infected with Piroplasmida small trophozoites, and A. marginale corpuscles were observed in erythrocytes from two animals. A. marginale and trophozoite co-infections occurred in two deer. A nested PCR analysis of the organs showed that six of the nine samples were positive for Theileria sp., five were positive for A. phagocytophilum and three were positive for A. marginale, with co-infection occurring in four deer. The results of the present study demonstrate that post-mortemdiagnostics using imprint slides and molecular assays are an effective method for detecting hemoparasites in organs.

scholarly journals Characterization of “Candidatus Ehrlichia Pampeana” in Haemaphysalis juxtakochi Ticks and Gray Brocket Deer (Mazama gouazoubira) from Uruguay

2021 ◽  
Vol 9 (10) ◽  
pp. 2165
Author(s):  
María Laura Félix ◽  
Sebastián Muñoz-Leal ◽  
Luis Andrés Carvalho ◽  
Diego Queirolo ◽  
Susana Remesar ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Pathogenic Role ◽  
Free Living ◽  
Epidemiological Factors ◽  
The Third ◽  
Mazama Gouazoubira ◽  
Potential Pathogenicity ◽  
Human Ehrlichiosis ◽  
Brocket Deer

Human ehrlichiosis are scantily documented in Uruguay. The aim of this study was to investigate the presence of Ehrlichia spp. in Haemaphysalis juxtakochi and in a gray brocket deer (Mazama gouazoubira) from Uruguay. The presence of Ehrlichia DNA was investigated in free-living H. juxtakochi in five localities of southeast and northeast Uruguay, as well as blood, spleen, and ticks retrieved from a M. gouazoubira. Ehrlichia spp. DNA was detected in six out of 99 tick pools from vegetation, in the spleen of M. gouazoubira, and in one out of five pools of ticks feeding on this cervid. Bayesian inference analyses for three loci (16S rRNA, dsb, and groEL) revealed the presence of a new rickettsial organism, named herein as “Candidatus Ehrlichia pampeana”. This new detected Ehrlichia is phylogenetically related to those found in ticks from Asia, as well as Ehrlichia ewingii from USA and Cameroon. Although the potential pathogenicity of “Ca. E. pampeana” for humans is currently unknown, some eco-epidemiological factors may be relevant to its possible pathogenic role, namely: (i) the phylogenetic closeness with the zoonotic agent E. ewingii, (ii) the evidence of H. juxtakochi parasitizing humans, and (iii) the importance of cervids as reservoirs for zoonotic Ehrlichia spp. The molecular detection of “Ca. E. pampeana” represents the third Ehrlichia genotype described in Uruguay.

scholarly journals Eco-Epidemiological Profile and Molecular Characterization of Simian Foamy Virus in a Recently-Captured Invasive Population of Leontopithecus chrysomelas (Golden-Headed Lion Tamarin) in Rio de Janeiro, Brazil

Viruses ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 931
Author(s):  
Miranda ◽  
Muniz ◽  
Moreira ◽  
Bueno ◽  
Kierulff ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Foamy Virus ◽  
Invasive Population ◽  
Free Living ◽  
Simian Foamy Virus ◽  
Old World ◽  
Leontopithecus Chrysomelas ◽  
Wide Range ◽  
Epidemiological Profile

Simian foamy viruses (SFV) infect a wide range of Old World and Neotropical primates (NP). Unlike Old World primates, little is known about the diversity and prevalence of SFV in NP, mainly from a free-living population. Phylogenetic analyses have shown that SFV coevolved with their hosts. However, viral strains infecting Leontopithecus chrysomelas did not behave as expected for this hypothesis. The purpose of this study was to determine the eco-epidemiological profile and molecular characterization of SFV in a recently captured invasive population of L. chrysomelas located in Niteroi/RJ using buccal swab as an alternative collection method. A prevalence of 34.8% (32/92) and a mean viral load of 4.7 log copies of SFV/106 cells were observed. With respect to time since capture, SFV prevalence was significantly higher in the group of animals sampled over 6 months after capture (55.2%) than in those more recently captured (25.4%) (p = 0.005). Infected solitary animals can contribute to SFV transmission between different groups in the population. SFV strains formed two distinct clades within the SFV infecting the Cebidae family. This is the first study to use buccal swabs as a tool to study SFV diversity and prevalence in a recently free-living NP population upon recent capture.

Isolation and molecular characterization of a Naegleria strain from a recreational water fountain in Tenerife, Canary Islands, Spain

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
María Reyes-Batlle ◽  
Carolina Wagner ◽  
Atteneri López-Arencibia ◽  
Ines Sifaoui ◽  
Enrique Martínez-Carretero ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Canary Islands ◽  
Recreational Water ◽  
Free Living ◽  
Many Sources ◽  
Soil And Water

AbstractFree-Living Amoebae (FLA) are widely distributed protozoa in the environment and have been isolated from many sources such as dust, soil and water. Among the pathogenic genera included in this group

scholarly journals Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy

2015 ◽  
Vol 12 (4) ◽  
pp. 3417-3427 ◽  
Author(s):  
Margherita Montalbano Di Filippo ◽  
Maristella Santoro ◽  
Piero Lovreglio ◽  
Rosa Monno ◽  
Carmen Capolongo ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Water Sources ◽  
Free Living ◽  
Different Water Sources

Ultrastructure of neuromuscular relationships in the canine heartworm (dirofilaria immitis)

1986 ◽  
Vol 44 ◽  
pp. 278-279
Author(s):  
W. L. Steffens ◽  
Nancy B. Roberts ◽  
J. M. Bowen
Keyword(s):  
Dirofilaria Immitis ◽  
Domestic Dogs ◽  
Structural Description ◽  
Pharmacological Effects ◽  
Free Living ◽  
Canine Heartworm ◽  
The World ◽  
Synaptic Region ◽  
Muscle Innervation ◽  
Insight Into

The canine heartworm is a common and serious nematode parasite of domestic dogs in many parts of the world. Although nematode neuroanatomy is fairly well documented, the emphasis has been on sensory anatomy and primarily in free-living soil species and ascarids. Lee and Miller reported on the muscular anatomy in the heartworm, but provided little insight into the peripheral nervous system or myoneural relationships. The classical fine-structural description of nematode muscle innervation is Rosenbluth's earlier work in Ascaris. Since the pharmacological effects of some nematacides currently being developed are neuromuscular in nature, a better understanding of heartworm myoneural anatomy, particularly in reference to the synaptic region is warranted.

1446: Molecular Characterization of Cyclooxygenase-2 in a Stimulated Prostate Stroma Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom
Keyword(s):  
Molecular Characterization ◽  
Cyclooxygenase 2 ◽  
Prostate Stroma
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