scholarly journals Isolation and Molecular Characterization of Free-Living Amoebae from Different Water Sources in Italy

2015 ◽  
Vol 12 (4) ◽  
pp. 3417-3427 ◽  
Author(s):  
Margherita Montalbano Di Filippo ◽  
Maristella Santoro ◽  
Piero Lovreglio ◽  
Rosa Monno ◽  
Carmen Capolongo ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Water Sources ◽  
Free Living ◽  
Different Water Sources

Incidence of free living amoeba in different water sources in el-minia governorate (middle egypt)

1998 ◽  
Vol 47 ◽  
pp. 159
Author(s):  
Khalifa M.A. Refaat ◽  
Gabr S. Nabil ◽  
Abedel-lateef Z.M. Manal
Keyword(s):  
Water Sources ◽  
Free Living ◽  
Free Living Amoeba ◽  
Different Water Sources

scholarly journals Isolation and molecular characterization of Acanthamoeba genotypes in recreational and domestic water sources from Jamaica, West Indies

2015 ◽  
Vol 13 (3) ◽  
pp. 909-919 ◽  
Author(s):  
Cheridah D. Todd ◽  
María Reyes-Batlle ◽  
José E. Piñero ◽  
Enrique Martínez-Carretero ◽  
Basilio Valladares ◽  
...  
Keyword(s):  
Water Sources ◽  
Skin Infections ◽  
Domestic Water ◽  
Free Living ◽  
Water Contact ◽  
First Report ◽  
The Caribbean ◽  
Polymerase Chain ◽  
Neurological Infections

Free living amoebae (FLA) are amphizoic protozoa that are ubiquitous in nature. Infection with FLA may result in neurological, ocular and skin infections. Exposure to Acanthamoeba occurs frequently through water contact and knowledge of the presence of the organisms in water sources is important in understanding transmission dynamics. The distribution of Acanthamoeba was studied in recreational and domestic water samples collected from across Jamaica. Morphological assessment and polymerase chain reaction revealed Acanthamoeba spp. isolates in 50.6% (42/83) and 17.3% (14/81) of recreational and domestic water, respectively. Sequencing of the DF3 region of the 18S rDNA resulted in the identification of genotypes T3, T4, T5, T10 and T11 corresponding to Acanthamoeba spp: A. griffini, A. triangularis, A. lenticulata, A. culbertsoni and A. hatchetti. Moreover, T4 was the most frequently isolated genotype in both recreational and domestic water. Thermotolerance and osmotolerance assays indicated that most isolates were potentially pathogenic. This is the first report of T3 and T10 genotypes in the Caribbean and the first report of these Acanthamoeba spp. in Jamaican waters. The study shows that there is potential risk of infection to contact wearers who practise poor lens care. Further, Acanthamoeba should be considered as a cause of neurological infections in Jamaica.

scholarly journals Prevalence and Characterization of Plasmid-mediated Quinolone Resistance Genes among Escherichia coli Strains Isolated from Different Water Sources in Alborz Province, Iran

2019 ◽  
Vol 11 (1) ◽  
pp. 36-41
Author(s):  
Reza Ranjbar ◽  
Shahrzad Tavanania ◽  
Azar Sabokbar ◽  
Faham Khamesipour
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Surface Water ◽  
Nalidixic Acid ◽  
Bacterial Species ◽  
Water Sources ◽  
Quinolone Resistance ◽  
E Coli ◽  
Different Water Sources

BACKGROUND: This study was conducted to investigate the prevalence of quinolone resistance associated (qnr) antibiotic resistance among Escherichia coli strains isolated from different water sources in Alborz province, Iran.METHODS: E. coli strains were isolated and identified by standard microbiological and biochemical tests from surface water sources in Alborz province, Iran in 2013. Fluoroquinolone-resistant isolates were determined using the antimicrobial susceptibility test determined by the Kirby–Bauer assay. Total genomic and plasmid DNA were extracted by boiling method. The presence of qnr genes in all nalidixic-acid and ciprofloxacin-resistant E. coli strains was determined by Polymerase Chain Reaction (PCR). The PCR amplicons were visualized after electrophoresis stained with ethidium bromide.RESULTS: One hundred E. coli strains were isolated from the water sources examined in this study. As much as 22.7% and 7.3% of the isolates were resistant to nalidixic acid and ciprofloxacin respectively. While qnrS, qnrB and qnrA genes were detected in 28%, 9% and 1% of fluoroquinolone-resistant isolates respectively. All fluoroquinolone-susceptible isolates however did not contain any of the qnr genes.CONCLUSION: This study reflects an increasing prevalence of fluoroquinolone-resistant E. coli strains in surface water sources. Underlining the importance of surface water sources as reservoirs for dissemination of potentially pathogenic E. coli and horizontal gene transfer between other waterborne bacterial species. Other possible mechanisms of resistance should also be investigated for better characterization of quinolone-resistant E. coli isolates. Therefore, immediate measures are needed to control and treat water sources more effectively.KEYWORDS: antibiotic resistance, E. coli, qnr genes, water sources 

scholarly journals Eco-Epidemiological Profile and Molecular Characterization of Simian Foamy Virus in a Recently-Captured Invasive Population of Leontopithecus chrysomelas (Golden-Headed Lion Tamarin) in Rio de Janeiro, Brazil

Viruses ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 931
Author(s):  
Miranda ◽  
Muniz ◽  
Moreira ◽  
Bueno ◽  
Kierulff ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Foamy Virus ◽  
Invasive Population ◽  
Free Living ◽  
Simian Foamy Virus ◽  
Old World ◽  
Leontopithecus Chrysomelas ◽  
Wide Range ◽  
Epidemiological Profile

Simian foamy viruses (SFV) infect a wide range of Old World and Neotropical primates (NP). Unlike Old World primates, little is known about the diversity and prevalence of SFV in NP, mainly from a free-living population. Phylogenetic analyses have shown that SFV coevolved with their hosts. However, viral strains infecting Leontopithecus chrysomelas did not behave as expected for this hypothesis. The purpose of this study was to determine the eco-epidemiological profile and molecular characterization of SFV in a recently captured invasive population of L. chrysomelas located in Niteroi/RJ using buccal swab as an alternative collection method. A prevalence of 34.8% (32/92) and a mean viral load of 4.7 log copies of SFV/106 cells were observed. With respect to time since capture, SFV prevalence was significantly higher in the group of animals sampled over 6 months after capture (55.2%) than in those more recently captured (25.4%) (p = 0.005). Infected solitary animals can contribute to SFV transmission between different groups in the population. SFV strains formed two distinct clades within the SFV infecting the Cebidae family. This is the first study to use buccal swabs as a tool to study SFV diversity and prevalence in a recently free-living NP population upon recent capture.

scholarly journals Detection and Molecular Characterization of Potentially Pathogenic Free-living Amoebae from Water Sources in Kish Island, Southern Iran

2015 ◽  
Vol 8s1 ◽  
pp. MBI.S24099 ◽  
Author(s):  
Maryam Niyyati ◽  
Zohreh Lasgerdi ◽  
Jacob Lorenzo-Morales
Keyword(s):  
Tap Water ◽  
18S Rrna Gene ◽  
Water Sources ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Free Living ◽  
Diagnostic Fragment ◽  
Amoebic Keratitis ◽  
Tourist Region

Amoebic keratitis, a sight-threatening corneal infection, mainly occurs in contact lens wearers who wash their eyes with tap water. The present research was conducted to identify the occurrence of potentially pathogenic free-living amoebae (FLA) in tap water sources on Kish Island, a tourist region in Iran. Amoebae were detected using a culture-enriched method and by polymerase chain reaction (PCR)/sequencing of the diagnostic fragment 3 region of the 18S rRNA gene of Acanthamoeba. In the case of other free-living amoebae species, PCR/sequencing analysis of the 18S rDNA was conducted. Results of this study showed the presence of Acanthamoeba belonging to T3, T4, T5, and T11 genotypes in tap water sources. Additionally, Vermamoebae vermiformis was detected in three water samples. This is the first report of the Acanthamoeba genotypes T3, T4, T5, and T11 and V. vermiformis species in tap water sources in a tourist region in Iran.

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