scholarly journals Effect of selenium and vitamin E supplementation on reproductive performance of young boars

2005 ◽  
Vol 48 (1) ◽  
pp. 68-75 ◽  
Author(s):  
A. Kołodziej ◽  
E. Jacyno
Keyword(s):  
Vitamin E ◽  
Seminal Plasma ◽  
Reproductive Performance ◽  
Morphological Changes ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Selenium Concentration ◽  
Control Group ◽  
Young Boars ◽  
Experimental Group

Abstract. The studies were carried out on 40 young boars of the synthetic line 990. On their 70 days of age, the boars were divided into two groups; the control group received 0.2 mg Se and 30 mg vitamin E while the experimental group received 0.5 mg Se + 60 mg vitamin E per 1 kg of feed mixtures. The feeding test was carried on from 70 days until 180 days of age. During the experiment the boars were subjected to live evaluations, i.e. testes volume, libido level, semen characteristics, as well as selenium concentration and glutathione peroxidase (GSH-Px) activity in blood serum and seminal plasma. The boars of the experimental group, compared with the control, showed significantly (P ≤ 0.05) higher sperm concentration and total sperm count, significantly lower (P ≤ 0.05) percentage of spermatozoa with major or minor morphological changes, elevated (P ≤ 0.05) percentage of spermatozoa with normal acrosome, and significantly higher (P ≤ 0.01) ORT values. GSH-Px activity was higher (P ≤ 0.05) in seminal plasma of the control group boars.

scholarly journals Influence of inorganic Se + vitamin E and organic Se + vitamin E on reproductive performance of young boars

2002 ◽  
Vol 11 (3) ◽  
pp. 175-184 ◽  
Author(s):  
E. JACYNO ◽  
M. KAWECKA ◽  
M. KAMYCZEK
Keyword(s):  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Seminal Plasma ◽  
Reproductive Performance ◽  
Morphological Changes ◽  
Selection Index ◽  
Osmotic Resistance ◽  
Spring Season ◽  
Semen Characteristics ◽  
Young Boars

The objective of the study was to compare the influence of the organic Se (Se-Yeast) + vitamin E and inorganic Se (Na2SeO3) + vitamin E on reproductive performance of young boars. The studies were carried on the 80 young boars. A feeding test was started on day 70 of their life. Inorganic Se group males received 0.2 mg inorganic Se + 30 mg vitamin E, those of organic Se group 0.2 mg organic Se + 60 mg vitamin E in 1 kg of the diet ration. The experiment was performed in two seasons: I - June-September, II - January-April. On day 180 of life (close of the test), the boars were subjected to live evaluation: testes volume, libido level, semen characteristics as well as Se content and glutathione peroxidase (GSH-Px) activity in seminal plasma and blood serum. The boars of group inorganic Se obtained higher (P = 0.01) selection index of live evaluation than those of the group organic Se. The boars of group organic Se were characterized by better libido level, higher (P = 0.05) concentration and total number of spermatozoa in an ejaculation, lower (P = 0.01) percentage of semen with morphological changes and higher (P = 0.01) value of osmotic resistance test of acrosome membranes in spite of the fact that GSH-Px activity in seminal plasma was lower when compared with those of the group inorganic Se. The findings show that organic Se + vitamin E has been of a more favourable influence on reproductive performance of young boars than that of inorganic Se + vitamin E. More favourable influence of organic Se + vitamin E was observed in winter-spring season than in summer.

scholarly journals Effects of coadministration of DHA and vitamin E on spermatogram, seminal oxidative stress, and sperm phospholipids in asthenozoospermic men: a randomized controlled trial

2020 ◽  
Vol 112 (3) ◽  
pp. 707-719 ◽  
Author(s):  
Ghazaleh Eslamian ◽  
Naser Amirjannati ◽  
Nazanin Noori ◽  
Mohammad-Reza Sadeghi ◽  
Azita Hekmatdoost
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Sperm Count ◽  
Controlled Trial ◽  
Sperm Concentration ◽  
Endocrine Function ◽  
Semen Parameters ◽  
Double Blind

ABSTRACT Background It is unknown which compounds in spermatozoa or seminal plasma may be involved in the regulation of sperm motility. Objectives The aim of this study was to investigate the effects of DHA (22:6n–3), vitamin E, and their probable interactions in men with asthenozoospermia. Methods A factorial, randomized, double-blind, placebo-controlled trial was conducted in infertility clinics in Tehran, Iran. The participants were idiopathic asthenozoospermic men aged 20–45 y, with normal endocrine function. Their concentration of spermatozoa and percentage of morphologically normal spermatozoa were equal to or above the lower reference limits, according to the fifth edition of the WHO guideline. Out of 717 men referred to the infertility clinics, 180 asthenozoospermic men were randomly assigned to 1 of 4 groups according to stratified blocked randomization by age and sperm concentration. Participants took daily 465 mg DHA plus 600 IU vitamin E (DE), 465 mg DHA plus placebo (DP), 600 IU vitamin E plus placebo (EP), or both placebo capsules (PP) for 12 wk. Sperm characteristics, oxidative stress of seminal plasma, serum and sperm membrane fatty acids, dietary intakes, anthropometric measurements, and physical activity were measured at baseline and after 12 wk. Results After the intervention, mean ± SD sperm progressive motility was greater in the DE group (27.9 ± 2.8) than in the DP (25.7 ± 3.4), EP (26.1 ± 2.8), and PP (25.8 ± 2.6) groups (P < 0.05). Sperm count (P = 0.001) and concentration (P = 0.044) increased significantly in the DE group compared with the other 3 groups, whereas other semen parameters were not significantly different between the groups after the intervention. Serum concentrations of n–3 PUFAs were significantly higher in the DE and DP groups than in the EP and PP groups. Conclusions Combined DHA and vitamin E supplements led to increased sperm motility; however, no significant changes occurred in sperm morphology and vitality in asthenozoospermic men. This trial was registered at clinicaltrials.gov as NCT01846325.

Effect of vitamin E on reproductive function in the mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin*

2008 ◽  
Vol 24 (9) ◽  
pp. 595-601 ◽  
Author(s):  
JP Xu ◽  
YP Yin ◽  
XQ Zhou
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Survival Rate ◽  
Sex Ratio ◽  
Pregnancy Rate ◽  
Reproductive Performance ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Experimental Group

The study was conducted to determine the effects of vitamin E on reproductive performance in mice treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The 75 female and 40 male mice were randomly assigned into five groups. The levels of both TCDD and vitamin E given by gavage were 0 and 0 (Control group), 100 and 0 (experimental group I), 100 and 20 (experimental group II), 100 and 100 (experimental group III), and 100 ng/kg/day and 500 mg/kg/day (experimental group IV), respectively. Males and females were mixed to mate at the ratio of 1:2 after 4-week experiment. The gavage treatments were continued until the end of gestation in female mice after mating. The results showed that the litter number, survival rate, and body weight at birth of offspring in experimental group I had significantly decreased, and the females’ pregnancy rate and pup sex ratio in experimental group I had the decreasing tendencies when compared with the control group. The litter number in experimental group III, survival rate in experimental group II and III, body weight at birth in experimental group III and IV exhibited significant increase compared with experimental group I. The female pregnancy rate in both experimental group III and IV recovered to 100%, but there were no significant differences when compared with experimental group I. The pups’ sex ratio had a gradually increasing tendency with increase of vitamin E level, but there was no significant difference among experimental group I–IV. The results suggest that TCDD could induce reproductive toxicity in mice, whereas vitamin E alleviated adverse effects on reproductive performance in mice caused by TCDD.

scholarly journals Effect of Selenium and Vitamin E Supplementation on Semen Quality in Dogs with Lowered Fertility

2015 ◽  
Vol 59 (1) ◽  
pp. 85-90 ◽  
Author(s):  
Anna Domosławska ◽  
Sławomir Zduńczyk ◽  
Wojciech Niżański ◽  
Andrzej Jurczak ◽  
Tomasz Janowski
Keyword(s):  
Vitamin E ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Control Group ◽  
Organic Selenium ◽  
Healthy Dogs ◽  
Manual Manipulation ◽  
Motility Parameters

Abstract Thirty clinically healthy dogs with poor semen quality were used in the study. Fifteen dogs were supplemented daily with selenium (0.6 mg/kg organic selenium from yeast) and vitamin E (5 mg/kg) per os for 60 d. The control group (15 dogs) was not supplemented. Semen was collected from all dogs by manual manipulation on days 0, 30, 60, and 90. The sperm concentration and motility parameters were evaluated with a Hamilton Thorne sperm analyser, version IVOS 12.3. For the assessment of sperm morphology, Diff-Quik stain was used. The percentage of live and dead spermatozoa was estimated on dried smears stained with eosin-nigrosin. The concentration of spermatozoa, most motility parameters determined (PMOT, VSL, VCL, ALH, BCF, RAPID, MEDIUM, SLOW, and STATIC), and the percentage of spermatozoa morphologically normal and live increased significantly (P < 0.05) after 60 d of supplementation. In the control group, there were no changes in motility parameters while the concentration and total sperm count decreased over the duration of the study. In conclusion, supplementation with selenium and vitamin E for 60 d can improve the quality of semen in dogs with lowered fertility.

scholarly journals Effect of L-Carnitine Supplementation on Boar Semen Quality

2007 ◽  
Vol 76 (4) ◽  
pp. 595-600 ◽  
Author(s):  
E. Jacyno ◽  
A. Kołodziej ◽  
M. Kamyczek ◽  
M. Kawęcka ◽  
K. Dziadek ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Morphological Changes ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Carnitine Supplementation ◽  
Boar Semen ◽  
Positive Effect ◽  
Plasma Activity

The effect of addition of L-carnitine on boar semen quality was studied in 5 Pietrain boars at age 1.5 - 2.0 years. The boars received 500 mg of L-carnitine per day for 5 weeks. During this period, their ejaculates were collected once a week and evaluated for quality. The control ejaculates had been collected before the application of L-carnitine. It was found that the addition of L-carnitine to the boars' feed had a positive effect on the quality of boar semen. The total ejaculate volume and sperm-rich fraction volume increased by 11% and 10%, respectively; the total ejaculate sperm count increased by 11.5% (P < 0.05). Also, the number of spermatozoa with major and minor morphological changes decreased and seminal plasma activity of AspAT was significantly reduced (P < 0.01). Sperm concentration and motility, as well as normal acrosome sperm percentage, did not increase considerably. The positive effect of L-carnitine on boar semen quality was observable as early as after one week of its application.

REACTION OF CD68-POSITIVE RAT LIVER AND SPLEEN CELLS ON SILICON INTAKE WITH DRINKING WATER

2021 ◽  
pp. 34-43
Author(s):  
Evgeniia A. Grigoreva ◽  
Valentina S. Gordova ◽  
Valentina E. Sergeeva ◽  
Alina T. Smorodchenko
Keyword(s):  
Drinking Water ◽  
Cytoplasmic Membrane ◽  
Unit Area ◽  
Morphological Changes ◽  
Morphological Characteristics ◽  
Control Group ◽  
Silicon Compound ◽  
Average Cell ◽  
Laboratory Rats ◽  
Experimental Group

The article presents data on the long-term effect (nine months) of a silicon compound supplied with drinking water – nonahydrate sodium metasilicate (10 mg/l in terms of silicon), on CD68-positive macrophages in the liver and spleen of laboratory rats. Changes in the morphological characteristics of this cell population were found. There was a decrease in the average cell area (in the liver of the control group of rats, the average macrophage area was 179.23±5.94 microns2, and in the group receiving silicon with drinking water – 117.04±3.35 microns2; in the spleen-136.02±3.93 microns2 and 103.44±2.8 microns2, respectively). Macrophages in the liver preparations of the experimental group of rats had a fewer processes and a darker cytoplasmic membrane. The number of macrophages in the liver per unit area was comparable, for the control group of rats it was 18.78±1.24, and for the rats that received with water with the addition of silicon – 19.41±0.75 cells. CD68+ macrophages of the red splenic pulp in laboratory rats that received silicon also underwent the following morphological changes: they were located in a denser way and had fewer processes, while the number of macrophages per unit area was 73.7±2.3 for the control group, 91.6±5.0-for the experimental group, respectively. The distance between them did not change. There was a change in the intensity of CD68 expression on the surface of the cytoplasmic membrane and in the cytoplasm of liver and spleen macrophages. These changes can be interpreted as the adaptive ability of liver and spleen macrophages to silicon introduced with drinking water. Given the heterogeneity of the macrophage population in the liver and spleen, further studies using markers for different subpopulations of macrophages are needed to clarify their role in the response of tissues to silicon supplied with drinking water.

scholarly journals Microelement Supplementation in Dairy Cows by Mineral Lick

2009 ◽  
Vol 78 (1) ◽  
pp. 29-36
Author(s):  
Štěpán Krys ◽  
Eva Lokajová ◽  
Aleš Podhorský ◽  
Leoš Pavlata
Keyword(s):  
Dairy Cows ◽  
Selenium Concentration ◽  
Control Group ◽  
Dry Period ◽  
Total Consumption ◽  
Zn Concentration ◽  
Ad Libitum ◽  
Mn Concentration ◽  
Experimental Group ◽  
Mineral Lick

The aim of this experiment was to verify the option of providing microelement supplementation to dairy cows in the dry period through supplemented mineral lick, and then to compare the content of microelements in the blood, colostrum and milk. The experiment was carried out on a farm with Czech Fleckvieh dairy cows. We formed an experimental group (E), supplied with mineral lick ad libitum enhanced with organic forms of microelements (Se, Zn, Cu, Mn) for four to five weeks before parturition and 1 week after parturition. The control group (C) did not receive any mineral lick. The use of mineral lick by cows was very uneven, its total consumption oscillated between 0 to 250 g of lick per animal per day. Lick feeding did not result in evidential increase of concentrations of monitored microelements in the cows’ blood, colostrum and milk. Only a tendency to increased concentrations of Se and Cu in the blood of group E of cows was observed; the average Se concentration increased by 60 μg l-1 (Cu by 9 μmol l-1), whereas in group C, Se increased only by 30 μg l-1 (Cu by 6 μg l-1). The concentration of microelements in colostrum after parturition dropped quickly. Significant drops were determined for Se, Cu and Zn on the first (for Mn on the second) day after parturition. Selenium concentration in the first colostrum was 5 times higher than in milk, Cu concentration 3 times higher, Zn concentration 4 times higher and Mn concentration 1.3 times higher.

scholarly journals Ameliorative effects of Spinacia oleracea on sperm morphology, count, and motility by normalizing the obesity-induced oxidative stress in Sprague Dawley rats

2020 ◽  
Vol 14 (03) ◽  
pp. 124-127
Author(s):  
Somia Iqbal ◽  
Noman Sadiq ◽  
Saad Siddiqui ◽  
Hira Iqbal
Keyword(s):  
Sperm Concentration ◽  
Treated Group ◽  
Sperm Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
Normal Morphology ◽  
Sprague Dawley Rats ◽  
Group A ◽  
Group B ◽  
Experimental Group

Background: Obesity is a prevailing metabolic disorder that affects the functioning of the male reproductive system. Excessive adipose tissue enhances reactive oxygen species generation and is linked with male infertility. Spinach has demonstrated antioxidant effects. The present study was conducted to determine the antioxidant effects of spinach on sperm parameters in obese Sprague Dawley rats. Subjects and methods: This randomized control study was conducted at the animal house of the National Institute of Health Islamabad, Islamic International Medical College, Cosmesurge International Hospital, Rawalpindi, and Apollo lab, Islamabad, Pakistan from April 2016 to March 2017. Forty male Sprague Dawley rats having an age of 8 weeks and weight 160-200g were tagged from number 1 to 40. Every third rat was randomly allocated to control Group A (n=13) and remaining into the Experimental group (n=27). Rats of control Group A was given a standard diet while a high-fat diet was given to Experimental group rats to induce obesity for the duration of six weeks. Weight (g) was measured weekly and obesity was confirmed when rats attain more than 20% weight when compared with that of rats of control Group A. Then, after obesity induction, the experimental group was alienated into the obesity control group (Group B) and spinach treated group (Group C). For sample, rats of Group A and Group B were sacrificed, and the cauda epididymis of each rat was placed in a Petri dish containing normal saline and cut into pieces to allow the release of sperm and then sperm parameters (sperms concentration, motility, and morphology) were recorded under the microscope. Then, spinach (5% hot water extract) along with the persistence of fat diet was administered to Group C for 4 weeks and finally, sperm parameters were measured in this group. Results: Sperm concentration/ml, motility (%), and normal morphology (%) of Group B rats were significantly decreased as compared to Group A rats. However, sperm concentration/ml, motility (%), and normal morphology (%) of Group C (spinach treated group) rats was significantly increased (p<0.001) as compared to Group B (obesity control group) rats after administering spinach. Conclusion: The addition of Spinach in a normal diet regimen restores normal sperm morphology, improves sperm motility and concentration.

scholarly journals ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ПОЛ ТА АКТИВНІСТЬ СИСТЕМИ АНТИОКСИДАНТНОГО ЗАХИСТУ У КРОВІ КОРОПІВ ЗА ДІЇ РІЗНОГО РІВНЯ ВІТАМІНУ Е І СЕЛЕНУ В РАЦІОНІ

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 201-204
Author(s):  
S.V. Yurchak ◽  
O.V. Derenj ◽  
O.I. Vishchur ◽  
Yu.M. Zabytivskyi
Keyword(s):  
Lipid Peroxidation ◽  
Vitamin E ◽  
Control Group ◽  
Fish Feed ◽  
Dose Dependent Decrease ◽  
Fat Soluble Vitamins ◽  
And Control ◽  
Dose Dependent ◽  
Experimental Group ◽  
Different Levels

The article consist data about effect of different levels of vitamin E and selenium in the diet of carps during their growing and also informationabout the influence on processes of lipid peroxidation and activity of antioxidant protection in their body.The experiment conducted in three experimental ponds. After wintering there were placed four ears mature females and males carp, six individuals in each group. Supplements of vitamin E administered at a rate of 25 mg/kg and drug «Sel–Plex» the rate of selenium – 0.3 mg/kg were added to further basic diet(BD) of female and male carp first experimental group (EG1). The second experimental group (EG2) received (BD) and vitamin E supplements in an amount of 75 mg / kg, and just as in EG1 – selenium – 0.3 mg/kg of feed.The control group received fish feed without additives vitamins and minerals.Feeding lasted for 30 days, due to the physiological needs of the fish feed. After spawning in fish of research and control group swere taken blood samples for biochemical research.The study led to a dose–dependent decrease (p < 0.01 – 0.001) content of TBA–active products and hydroperoxidase of lipids, but did not significantly effect onsuperoxide dismutase and glutathione peroxidase activity of blood So, the reduction of lipid peroxidation products in the carp’s blood of experimental groups probably was caused by the growth of non–enzymatic level, which is associated with fat–soluble vitamins. 

scholarly journals Pengaruh Pemberian Antioksidan Vitamin C Dan E Terhadap Konsentrasi Spermatozoa Mencit (Mus Musculus) Yang Dipapar Asap Rokok

2017 ◽  
Vol 5 (1) ◽  
pp. 26
Author(s):  
Ervina Wati Harahap ◽  
Normalina Sandora ◽  
Winarto Winarto
Keyword(s):  
Reactive Oxygen Species ◽  
Free Radical ◽  
Vitamin E ◽  
Vitamin C ◽  
Male Infertility ◽  
Research Design ◽  
Mus Musculus ◽  
Sperm Count ◽  
Control Group ◽  
Oxygen Species

Free radical that are released in cigarette smoke, believed to play an important role in male infertility. The free radicalproduces Reactive Oxygen Species (ROS) that is found increased in male smokers. ROS is one of the free radicalscomponent can be neutralized by vitamin C and E as an antioxidant. This study using those agents to the fifteen minuteshalf piece cigarette to expose mice and count their sperm as the fertilility parameter. The research design used was posttest-only control group with twenty mice were divided into five groups. A group of no smoke exposed was categorizedas a baseline control, the exposed groups were consisted of one group without antioxidant, one group with vitamin Ctreatment, a group of vitamin E treatment and at last the combination of vitamin C and E. All groups were treatedfor fourteen days. The results were analyzed using oneway ANOVA with p<0.05. It is obviously proven that thecombination of vitamin C and vitamin E treatment on the usual dose gave the highest sperm count compared to othergroups.

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