A new screening method for identifying chemosensory receptors responding to agonist

2021 ◽  
Author(s):  
Sakura Tsuchiya ◽  
Yuko Terada ◽  
Minami Matsuyama ◽  
Toyomi Yamazaki-Ito ◽  
Keisuke Ito
Keyword(s):  
Screening Method ◽  
New Method ◽  
Chemical Substances ◽  
Chemosensory Receptor ◽  
Chemosensory Receptors ◽  
Taste And Odor ◽  
Receptor Interactions ◽  
Taste And Smell ◽  
Simple Screening

ABSTRACT Humans sense taste and smell of various chemical substances through approximately 430 chemosensory receptors. The overall picture of ligand–chemosensory receptor interactions has been partially clarified because of numerous interactions. This study presents a new method that enables a rapid and simple screening of chemosensory receptors. It would be useful for identifying chemosensory receptors activated by taste and odor substances.

A PATCH TEST FOR CHLORIDE IN SWEAT AS A SIMPLE SCREENING METHOD FOR DETECTING CYSTIC FIBROSIS OF THE PANCREAS

PEDIATRICS ◽  
1959 ◽  
Vol 23 (4) ◽  
pp. 731-737
Author(s):  
Louis Gluck
Keyword(s):  
Cystic Fibrosis ◽  
Patch Test ◽  
Screening Test ◽  
Screening Method ◽  
Diagnostic Methods ◽  
Simple Screening

A simple patch test, designed as a screening test for cystic fibrosis of the pancreas, is reported in preliminary form. In trials carried out with 207 patients it has proved accurate and reliable. A brief resume of the physiology of sweating, especially as pertinent to diagnostic methods in pediatrics, is also presented. Studies are in progress aiming toward further refinement of this method.

scholarly journals A Machine Learning Application Based in Random Forest for Integrating Mass Spectrometry-Based Metabolomic Data: A Simple Screening Method for Patients With Zika Virus

2018 ◽  
Vol 6 ◽  
Author(s):  
Carlos Fernando Odir Rodrigues Melo ◽  
Luiz Claudio Navarro ◽  
Diogo Noin de Oliveira ◽  
Tatiane Melina Guerreiro ◽  
Estela de Oliveira Lima ◽  
...  
Keyword(s):  
Machine Learning ◽  
Mass Spectrometry ◽  
Random Forest ◽  
Zika Virus ◽  
Screening Method ◽  
Metabolomic Data ◽  
Simple Screening

Detection of the Na+-translocating NADH-quinone reductase in marine bacteria using a PCR technique

2000 ◽  
Vol 46 (4) ◽  
pp. 325-332 ◽  
Author(s):  
Sanae Kato ◽  
Isao Yumoto
Keyword(s):  
Phylogenetic Analysis ◽  
Amino Acid ◽  
16S Rrna ◽  
Marine Bacteria ◽  
Genomic Dna ◽  
Screening Method ◽  
Quinone Reductase ◽  
Amino Acid Sequences ◽  
Homologous Sequences ◽  
Simple Screening

To examine the distribution of the Na+-translocating NADH-quinone reductase (Na+-NQR) among marine bacteria, we developed a simple screening method for the detection of this enzyme. By reference to the homologous sequences of the Na+-NQR operons from Vibrio alginolyticus and Haemophilus influenzae, a pair of primers was designed for amplification of a part of the sixth ORF (nqr6) of the Na+-NQR operon. When PCR was performed using genomic DNA from 13 marine bacteria, a 0.9-kbp fragment corresponding to nqr6 was amplified in 10 strains. Although there were three PCR-negative strains phylogenetically, based on the sequence of the 16S rRNA, these were placed far from the PCR-positive strains. No product was observed in the case of nonmarine bacteria. The nucleotide and predicted amino acid sequences of nqr6 were highly conserved among the PCR-positive marine bacteria. A phylogenetic analysis of marine bacteria, based on nqr6 sequencing, was performed.Key words: Na+-translocating, NADH-quinone reductase, marine bacteria, PCR.

scholarly journals A rapid and simple screening method for methamphetamine in urine by radioimmunoassay using a 125I-labeled methamphetamine derivative.

1980 ◽  
Vol 28 (9) ◽  
pp. 2779-2782 ◽  
Author(s):  
SEIICHI INAYAMA ◽  
YUKIKO TOKUNAGA ◽  
EIKICHI HOSOYA ◽  
TERUO NAKADATE ◽  
TETSUKICHI NIWAGUCHI ◽  
...  
Keyword(s):  
Screening Method ◽  
Simple Screening
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