scholarly journals Egg-induced Changes in Serum Lipids Are Associated with Clinical Immune Cell Counts (OR12-04-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Catherine Andersen ◽  
Christa Palancia Esposito ◽  
Julia Greco ◽  
Allison Sloan ◽  
Aaron Van Dyke
Keyword(s):  
Blood Cell ◽  
Total Cholesterol ◽  
Serum Lipids ◽  
Immune Cell ◽  
Hdl Cholesterol ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Absolute Lymphocyte Counts ◽  
Induced Changes ◽  
Whole Egg

Abstract Objectives We have previously demonstrated that serum lipids can predict clinical immune cell counts at the population level; however, it is unknown whether diet-induced changes in serum lipids correspond to similar shifts in clinical blood cell counts. We hypothesized that whole egg vs. egg white consumption, which is known to differentially affect lipoprotein profiles and inflammatory markers, would induce shifts in clinical immune cells counts that are associated with changes in serum lipids. Methods In this ongoing study, healthy men and women (18–35y, BMI < 30 kg/m2, n = 11) consumed an egg-free diet for 4 weeks, followed by a 4-week diet containing either 3 whole eggs or 3 egg whites per day. Fasting serum lipids and complete blood cell counts were measured at the end of each diet period. Results Following the egg-free diet period, individuals with higher total cholesterol levels had greater absolute lymphocyte counts, and a trend toward greater absolute eosinophils counts. While no significant changes in total cholesterol or LDL-cholesterol were observed between diet periods, HDL-cholesterol was increased in subjects consuming whole eggs only. Similarly, serum triglycerides, alanine aminotransferase, and platelet counts were only decreased by whole egg intake. Interestingly, while egg intake did not alter total white blood counts, there was a trend toward decreased absolute lymphocyte counts in all subjects following consumption of both whole eggs and egg whites, as compared to the egg-free diet period. Across all subjects, a strong positive correlation was observed between changes in HDL-cholesterol vs. changes in absolute monocytes, as well as the percentage of monocytes in total white blood cell counts. Changes in triglycerides were negatively associated with changes in eosinophil levels. Conclusions These findings suggest that egg-induced changes in serum lipids are associated with differential shifts in clinical immune cell counts. Funding Sources This study was funded by an Agriculture and Food Research Initiative Grant from the USDA National Institute of Food and Agriculture.

scholarly journals Changes in Serum Lipids from Egg Consumption Are Associated with Clinical Erythrocyte Indices (P08-093-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Julia Greco ◽  
Allison Sloan ◽  
Christa Palancia Esposito ◽  
Aaron Van Dyke ◽  
Catherine Andersen
Keyword(s):  
Blood Cell ◽  
Total Cholesterol ◽  
Red Blood Cell ◽  
Serum Lipids ◽  
Hdl Cholesterol ◽  
Distribution Width ◽  
Erythrocyte Indices ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Induced Changes

Abstract Objectives Serum lipids may influence the lifespan of erythrocytes, and have been associated with clinical erythrocyte indices at the population level. Consumption of whole eggs vs. egg whites exerts varying effects on serum lipids and lipoprotein profiles; therefore, we investigated whether egg-induced changes in serum lipids correspond to changes in clinical erythrocyte markers. Methods Young, healthy men and women (18–35y, BMI < 30 kg/m2, n = 11) were recruited to participate in an ongoing intervention trial. All subjects followed an egg-free diet for 4 weeks, then were randomized to consume either 3 whole eggs or 3 egg whites per day for 4 weeks. Fasting serum lipids, complete blood cell counts, and dietary intake analysis was performed at the end of each study period. Results Average serum lipids and erythrocyte indices were within normal ranges at the end of each diet period. Changes in total cholesterol following the whole egg (+4.8%) and egg white (0.4%) diet period were not significant as compared to the egg-free diet period. Similarly, clinical erythrocyte markers were not significantly altered by daily consumption of whole eggs or egg whites. However, changes in total cholesterol and LDL-cholesterol between diet periods were positively correlated with changes in total red blood cell counts and hemoglobin. Total cholesterol was additionally correlated with hematocrit levels, and negatively associated with red cell distribution width. Total red blood cell counts and hemoglobin were further correlated with changes in the total cholesterol to HDL-cholesterol ratios, but not HDL-cholesterol alone. Conclusions Our findings indicate that egg-induced changes in serum lipids are associated with clinical erythrocyte indices, and that total cholesterol levels and dyslipidemia may be more significant determinants of erythrocyte profiles. Funding Sources This study was funded by an Agriculture and Food Research Initiative Grant from the USDA National Institute of Food and Agriculture.

scholarly journals Low HDL (High-Density Lipoprotein) Cholesterol and High White Blood Cell Counts

2020 ◽  
Author(s):  
Mads Harsløf ◽  
Kasper M. Pedersen ◽  
Børge G. Nordestgaard ◽  
Shoaib Afzal
Keyword(s):  
Blood Cell ◽  
High Density Lipoprotein ◽  
White Blood Cell ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
High Density ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Low Hdl ◽  
White Blood Cell Counts

Objective: Animal studies suggest that HDL (high-density lipoprotein) regulates proliferation and differentiation of hematopoietic stem cells. Using a Mendelian randomization approach, we tested the hypothesis that low HDL cholesterol is associated with high white blood cell counts. Approach and Results: We included 107 952 individuals aged 20 to 100 years from the Copenhagen General Population Study with information on HDL cholesterol, white blood cell counts, and 9 genetic variants associated with HDL cholesterol. In multivariable-adjusted observational analyses, HDL cholesterol was inversely associated with white blood cell counts. On a continuous scale, a 1-mmol/L (39 mg/dL) lower HDL cholesterol was associated with 5.1% (95% CI, 4.7%–5.4%) higher leukocytes, 4.5% (95% CI, 4.0%–4.9%) higher neutrophils, 5.7% (95% CI, 5.3%–6.1%) higher lymphocytes, 5.7% (95% CI, 5.3%–6.2%) higher monocytes, 14.8% (95% CI, 13.9%–15.8%) higher eosinophils, and 3.9% (95% CI, 3.1%–4.7%) higher basophils. In age- and sex-adjusted genetic analyses using the inverse-variance weighted analysis, a 1-mmol/L (39 mg/dL) genetically determined lower HDL cholesterol was associated with 2.2% (95% CI, 0.3%–4.1%) higher leukocytes, 4.3% (95% CI, 1.6%–7.1%) higher lymphocytes, 4.3% (95% CI, 2.6%–6.1%) higher monocytes, and 4.8% (95% CI, 1.2%–8.5%) higher eosinophils. Overall, the genetic associations were robust across sensitivity analyses and replicated using summary statistics from the UK Biobank with up to 350 470 individuals. Conclusions: Genetic and hence lifelong low HDL cholesterol was associated with high peripheral blood leukocytes, including high lymphocytes, monocytes, and eosinophils. The concordance between observational and genetic estimates and independent replication suggest a potential causal relationship.

Hyperventilation-induced changes of blood cell counts depend on hypocapnia

1994 ◽  
Vol 69 (5) ◽  
pp. 402-407 ◽  
Author(s):  
M. St�ubli ◽  
F. Vogel ◽  
P. B�rtsch ◽  
G. Fl�ckiger ◽  
W. H. Ziegler
Keyword(s):  
Blood Cell ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Induced Changes

scholarly journals Remote immune processes revealed by immune-derived circulating cell-free DNA

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Ilana Fox-Fisher ◽  
Sheina Piyanzin ◽  
Bracha Lea Ochana ◽  
Agnes Klochendler ◽  
Judith Magenheim ◽  
...  
Keyword(s):  
Blood Cell ◽  
B Cell ◽  
Immune Cell ◽  
B Cell Lymphoma ◽  
Cell Types ◽  
Cell Free Dna ◽  
Cell Counts ◽  
Free Dna ◽  
Blood Cell Counts ◽  
Circulating Cell Free Dna

Blood cell counts often fail to report on immune processes occurring in remote tissues. Here we use immune cell type-specific methylation patterns in circulating cell-free DNA (cfDNA) for studying human immune cell dynamics. We characterized cfDNA released from specific immune cell types in healthy individuals (N=242), cross sectionally and longitudinally. Immune cfDNA levels had no individual steady state as opposed to blood cell counts, suggesting that cfDNA concentration reflects adjustment of cell survival to maintain homeostatic cell numbers. We also observed selective elevation of immune-derived cfDNA upon perturbations of immune homeostasis. Following influenza vaccination (N=92), B-cell-derived cfDNA levels increased prior to elevated B-cell counts and predicted efficacy of antibody production. Patients with Eosinophilic Esophagitis (N=21) and B-cell lymphoma (N=27) showed selective elevation of eosinophil and B-cell cfDNA respectively, which were undetectable by cell counts in blood. Immune-derived cfDNA provides a novel biomarker for monitoring immune responses to physiological and pathological processes that are not accessible using conventional methods.

scholarly journals The association of plasma lipids with white blood cell counts: Results from the Multi-Ethnic Study of Atherosclerosis

2018 ◽  
Author(s):  
Yong Chang Lai ◽  
Kevin J. Woollard ◽  
Robyn L. McClelland ◽  
Matthew A. Allison ◽  
Kerry-Anne Rye ◽  
...  
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Ldl Cholesterol ◽  
Plasma Lipids ◽  
Hdl Cholesterol ◽  
Lipid Lowering ◽  
Total White Blood Cell ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
White Blood Cell Counts

AbstractBackground and aimsPrevious studies have demonstrated that elevated cholesterol results in increased white blood cell counts in mouse models. However, there is insufficient evidence to support this in humans. We, therefore, investigated the relationship of plasma lipids with white blood cell counts (basophils, eosinophils, monocytes, neutrophils and lymphocytes) in the Multi-Ethnic Study of Atherosclerosis (MESA).MethodsThe analysis included 2873 MESA participants at visit 5 with a complete white blood count and differential analysis. The cross-sectional association of total cholesterol, LDL cholesterol, HDL cholesterol and triglyceride levels with different white blood cell counts was analyzed by multivariable linear regression.ResultsAfter adjusting sociodemographic and confounding factors including red blood cells counts, platelet counts, use of lipid-lowering medication, CVD risk factors and other lipid measures, and multiple testing correction, a 1-SD increment in total cholesterol and LDL cholesterol was associated with 2.8% and 2.3% (both p<0.001) lower total white blood cell counts. The same increment in ln-transformed triglyceride levels was associated with 2.3% higher total white blood cell counts, 2.9% higher lymphocyte counts and 2.7% lower monocyte counts (all p<0.001). HDL cholesterol was not associated with any white blood cell counts. Similar results were obtained after excluding participants taking lipid-lowering medication.ConclusionsWhilst significant associations were observed, the heterogenous and modest nature of the relationships between plasma lipid levels and white blood cell populations make it hard to support the hypothesis that lipids are in the causal pathway for leukogenesis.

scholarly journals Associations between absolute neutrophil count and lymphocyte-predominant breast cancer

2019 ◽  
Author(s):  
Chang Ik Yoon ◽  
So Eun Park ◽  
Yoon Jin Cha ◽  
Soong June Bae ◽  
Chi Hwan Cha ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Blood Cell ◽  
Peripheral Blood ◽  
Immune Cell ◽  
Laboratory Data ◽  
Peripheral Blood Cell ◽  
Cell Mediated Immunity ◽  
Breast Cancer Patients ◽  
Cell Counts ◽  
Blood Cell Counts

AbstractTumor-infiltrating lymphocytes (TILs) might be associated with host-cell mediated immunity, which could be partly reflected by peripheral blood cell counts. We aimed to investigate whether peripheral blood cell counts are associated with TILs in breast cancer. Between August 2016 and July 2018, we evaluated the percentage of stromal TILs in breast cancer patients who underwent primary surgery, using the standardized methodology proposed by the international TIL Working Group. Lymphocyte-predominant breast cancer (LPBC) was defined as tumors having high TIL levels (≥ 50%). Peripheral blood cell counts including absolute neutrophil counts (ANC), absolute lymphocyte counts (ALC) and neutrophil-to-lymphocyte ratio (NLR) was obtained from pretreatment laboratory data. Of the 684 patients, 99 (17.2%) had LPBC, and 478 (82.8%) had non-LBPC. In a comparison of 3 markers of peripheral blood counts, LPBC had a significantly lower mean ANC than non-LPBC (3,330 vs. 3,660; P=0.004), but the other means were not different. Decreasing ANC was an independent clinical factor in predicting LPBC (OR: 0.736, 95% CI: 0.591-0.917; P=0.004). Low peripheral ANC might be linked with LPBC, supporting the hypothesis that systemic immune cell counts might be associated with the tumor-immune microenvironment.

scholarly journals Remote immune processes revealed by immune-derived circulating cell-free DNA

2021 ◽  
Author(s):  
Ilana Fox-Fisher ◽  
Sheina Piyanzin ◽  
Agnes Klochendler ◽  
Bracha Lea Ochana ◽  
Judith Magenheim ◽  
...  
Keyword(s):  
Blood Cell ◽  
B Cell ◽  
Immune Cell ◽  
B Cell Lymphoma ◽  
Cell Types ◽  
Cell Free Dna ◽  
Cell Counts ◽  
Free Dna ◽  
Blood Cell Counts ◽  
Circulating Cell Free Dna

Blood cell counts often fail to report on immune processes occurring in remote tissues. Here we use immune cell type-specific methylation patterns in circulating cell-free DNA (cfDNA) for studying human immune cell dynamics. We characterized cfDNA released from specific immune cell types in healthy individuals (N=242), cross sectionally and longitudinally. Immune cfDNA levels had no individual steady state as opposed to blood cell counts, suggesting that cfDNA concentration reflects adjustment of cell survival to maintain homeostatic cell numbers. We also observed selective elevation of immune-derived cfDNA upon perturbations of immune homeostasis. Following influenza vaccination (N=92), B-cell-derived cfDNA levels increased prior to elevated B-cell counts and predicted efficacy of antibody production. Patients with Eosinophilic Esophagitis (N=21) and B-cell lymphoma (N=27) showed selective elevation of eosinophil and B-cell cfDNA respectively, which were undetectable by cell counts in blood. Immune-derived cfDNA provides a novel biomarker for monitoring immune responses to physiological and pathological processes that are not accessible using conventional methods.

An Alternative Elastase-mediated Degradation of Fibrinogen and Fibrin Observed in a Patient with Herpes Simplex Encephalitis and Pneumonia

1996 ◽  
Vol 76 (02) ◽  
pp. 184-186 ◽  
Author(s):  
Kenji lijima ◽  
Fumiyo Murakami ◽  
Yasushi Horie ◽  
Katsumi Nakamura ◽  
Shiro Ikawa ◽  
...  
Keyword(s):  
Herpes Simplex ◽  
Blood Cell ◽  
White Blood Cell ◽  
Herpes Simplex Encephalitis ◽  
Pmn Elastase ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Sds Page ◽  
White Blood Cell Counts ◽  
Pmn Élastase

SummaryA 74-year-old female developed pneumonia following herpes simplex encephalitis. Her white blood cell counts reached 28,400/μl, about 90% of which consisted of granulocytes. The polymorphonuclear (PMN) elastase/α1-arantitrypsin complex levels increased and reached the maximum of 5,019 ng/ml, indicating the release of a large amount of elastase derived from the granulocytes. The mechanism of PMN elastase release was most likely to be granulocyte destruction associated with phagocytosis. The cleavage of fibrinogen and fibrin by PMN elastase, independent of plasmin, was indicated by the presence of the fragments in immunoprecipitated plasma from the patient corresponding to elastase-induced FDP D and DD fragments and the absence of fragments corresponding to plasmin-induced FDP D and DD fragments on SDS-PAGE. These findings suggested that the large amount of PMN elastase released from the excessive numbers of granulocytes in this patient with herpes simplex encephalitis and pneumonia, induced the cleavage of fibrinogen and fibrin without the participation of plasmin.

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