ALLIUM SATIVUM ESSENTIAL OIL (ASEO); EFFECT OF SUPPLEMENTATION ON SERUM TRIGLYCERIDES, TOTAL CHOLESTEROL, HDLC, LDLC AND BLOOD CELL COUNTS IN ALBINO RATS

Objectives: To determine the effects of Allium sativum essential oil (ASEO)phytotherapy on serum triglycerides, total cholesterol, HDLc, LDLc and blood cell counts inalbino rat model. Study design: Experimental study. Setting and Duration: Animal House,Sindh Agriculture University and Isra University Hyderabad from May 2014 to January 2015.Materials and Methods: 60 albino rats were divided into four groups. Controls were givenPlacebo. Experimental rat groups were given ASEO 100 mg/kg, 200 mg/kg and 300 mg/kgorally for 30 days. Cardiac puncture was performed for blood sampling. Research variableswere analyzed on Statistix 10.0 (USA). Results: Blood lipids showed significant reduction invarious blood lipid fractions. Serum LDLc exhibited with a concomitant rise in serum HDLc (p=0.0001) in high ASEO treated rats. Red blood cells, white blood cells and platelet showedsignificant improvement ASEO fed rats (p=0.001). Conclusion: Allium sativum essential oil(ASEO) phytotherapy showed a rise in HDLc and a reduction in LDLc, triglycerides and totalcholesterol with improvement in red blood cell counts.

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Egg-induced Changes in Serum Lipids Are Associated with Clinical Immune Cell Counts (OR12-04-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz049.or12-04-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Catherine Andersen ◽

Christa Palancia Esposito ◽

Julia Greco ◽

Allison Sloan ◽

Aaron Van Dyke

Keyword(s):

Blood Cell ◽

Total Cholesterol ◽

Serum Lipids ◽

Immune Cell ◽

Hdl Cholesterol ◽

Cell Counts ◽

Blood Cell Counts ◽

Absolute Lymphocyte Counts ◽

Induced Changes ◽

Whole Egg

Abstract Objectives We have previously demonstrated that serum lipids can predict clinical immune cell counts at the population level; however, it is unknown whether diet-induced changes in serum lipids correspond to similar shifts in clinical blood cell counts. We hypothesized that whole egg vs. egg white consumption, which is known to differentially affect lipoprotein profiles and inflammatory markers, would induce shifts in clinical immune cells counts that are associated with changes in serum lipids. Methods In this ongoing study, healthy men and women (18–35y, BMI < 30 kg/m2, n = 11) consumed an egg-free diet for 4 weeks, followed by a 4-week diet containing either 3 whole eggs or 3 egg whites per day. Fasting serum lipids and complete blood cell counts were measured at the end of each diet period. Results Following the egg-free diet period, individuals with higher total cholesterol levels had greater absolute lymphocyte counts, and a trend toward greater absolute eosinophils counts. While no significant changes in total cholesterol or LDL-cholesterol were observed between diet periods, HDL-cholesterol was increased in subjects consuming whole eggs only. Similarly, serum triglycerides, alanine aminotransferase, and platelet counts were only decreased by whole egg intake. Interestingly, while egg intake did not alter total white blood counts, there was a trend toward decreased absolute lymphocyte counts in all subjects following consumption of both whole eggs and egg whites, as compared to the egg-free diet period. Across all subjects, a strong positive correlation was observed between changes in HDL-cholesterol vs. changes in absolute monocytes, as well as the percentage of monocytes in total white blood cell counts. Changes in triglycerides were negatively associated with changes in eosinophil levels. Conclusions These findings suggest that egg-induced changes in serum lipids are associated with differential shifts in clinical immune cell counts. Funding Sources This study was funded by an Agriculture and Food Research Initiative Grant from the USDA National Institute of Food and Agriculture.

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Changes in Serum Lipids from Egg Consumption Are Associated with Clinical Erythrocyte Indices (P08-093-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz044.p08-093-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Julia Greco ◽

Allison Sloan ◽

Christa Palancia Esposito ◽

Aaron Van Dyke ◽

Catherine Andersen

Keyword(s):

Blood Cell ◽

Total Cholesterol ◽

Red Blood Cell ◽

Serum Lipids ◽

Hdl Cholesterol ◽

Distribution Width ◽

Erythrocyte Indices ◽

Cell Counts ◽

Blood Cell Counts ◽

Induced Changes

Abstract Objectives Serum lipids may influence the lifespan of erythrocytes, and have been associated with clinical erythrocyte indices at the population level. Consumption of whole eggs vs. egg whites exerts varying effects on serum lipids and lipoprotein profiles; therefore, we investigated whether egg-induced changes in serum lipids correspond to changes in clinical erythrocyte markers. Methods Young, healthy men and women (18–35y, BMI < 30 kg/m2, n = 11) were recruited to participate in an ongoing intervention trial. All subjects followed an egg-free diet for 4 weeks, then were randomized to consume either 3 whole eggs or 3 egg whites per day for 4 weeks. Fasting serum lipids, complete blood cell counts, and dietary intake analysis was performed at the end of each study period. Results Average serum lipids and erythrocyte indices were within normal ranges at the end of each diet period. Changes in total cholesterol following the whole egg (+4.8%) and egg white (0.4%) diet period were not significant as compared to the egg-free diet period. Similarly, clinical erythrocyte markers were not significantly altered by daily consumption of whole eggs or egg whites. However, changes in total cholesterol and LDL-cholesterol between diet periods were positively correlated with changes in total red blood cell counts and hemoglobin. Total cholesterol was additionally correlated with hematocrit levels, and negatively associated with red cell distribution width. Total red blood cell counts and hemoglobin were further correlated with changes in the total cholesterol to HDL-cholesterol ratios, but not HDL-cholesterol alone. Conclusions Our findings indicate that egg-induced changes in serum lipids are associated with clinical erythrocyte indices, and that total cholesterol levels and dyslipidemia may be more significant determinants of erythrocyte profiles. Funding Sources This study was funded by an Agriculture and Food Research Initiative Grant from the USDA National Institute of Food and Agriculture.

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The effect of aqueous extract of Carica papaya leaves on liver enzymes and blood cell counts of normal albino rats

International Journal of Biological and Chemical Sciences ◽

10.4314/ijbcs.v3i3.45349 ◽

2009 ◽

Vol 3 (3) ◽

Author(s):

BI Nwiloh ◽

NM Nwinuka ◽

MO Monanu

Keyword(s):

Blood Cell ◽

Aqueous Extract ◽

Carica Papaya ◽

Liver Enzymes ◽

Albino Rats ◽

Cell Counts ◽

Blood Cell Counts

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An Alternative Elastase-mediated Degradation of Fibrinogen and Fibrin Observed in a Patient with Herpes Simplex Encephalitis and Pneumonia

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650550 ◽

1996 ◽

Vol 76 (02) ◽

pp. 184-186 ◽

Cited By ~ 2

Author(s):

Kenji lijima ◽

Fumiyo Murakami ◽

Yasushi Horie ◽

Katsumi Nakamura ◽

Shiro Ikawa ◽

...

Keyword(s):

Herpes Simplex ◽

Blood Cell ◽

White Blood Cell ◽

Herpes Simplex Encephalitis ◽

Pmn Elastase ◽

Cell Counts ◽

Blood Cell Counts ◽

Sds Page ◽

White Blood Cell Counts ◽

Pmn Élastase

SummaryA 74-year-old female developed pneumonia following herpes simplex encephalitis. Her white blood cell counts reached 28,400/μl, about 90% of which consisted of granulocytes. The polymorphonuclear (PMN) elastase/α1-arantitrypsin complex levels increased and reached the maximum of 5,019 ng/ml, indicating the release of a large amount of elastase derived from the granulocytes. The mechanism of PMN elastase release was most likely to be granulocyte destruction associated with phagocytosis. The cleavage of fibrinogen and fibrin by PMN elastase, independent of plasmin, was indicated by the presence of the fragments in immunoprecipitated plasma from the patient corresponding to elastase-induced FDP D and DD fragments and the absence of fragments corresponding to plasmin-induced FDP D and DD fragments on SDS-PAGE. These findings suggested that the large amount of PMN elastase released from the excessive numbers of granulocytes in this patient with herpes simplex encephalitis and pneumonia, induced the cleavage of fibrinogen and fibrin without the participation of plasmin.

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