A Simple Method for the Determination of Lipoprotein Lipase

1968 ◽  
Vol 14 (10) ◽  
pp. 1023-1025
Author(s):  
Leonard J Stutman ◽  
Marilyn Dolliver
Keyword(s):  
Fatty Acids ◽  
Lipoprotein Lipase ◽  
Fatty Acid Esters ◽  
Simple Method ◽  
Intravenous Heparin ◽  
Dense Band ◽  
Layer Chromatography ◽  
Acid Esters

Abstract A rapid method for determining the in-vivo effect of small amounts of intravenous heparin utilizing thin-layer chromatography is presented. A dense band of stainable fatty acids appears and represents nonesterified acids after the fatty acid esters have been hydrolyzed by lipoprotein lipase and determines, therefore, whether the enzyme is activated by heparin.

Gas-Liquid Chromatographic Determination of Sucrose Fatty Acid Esters

1983 ◽  
Vol 66 (4) ◽  
pp. 1050-1052
Author(s):  
Taizo Tsuda ◽  
Hiroshi Nakanishi
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Methyl Esters ◽  
Chromatographic Determination ◽  
Fatty Acid Esters ◽  
Liquid Chromatographic Determination ◽  
Sucrose Fatty Acid Esters ◽  
Liquid Chromatographic ◽  
Acid Esters

Abstract A method was developed for gas-liquid chromatographic determination of sucrose fatty acid esters as TMS derivative of sucrose and methyl esters of fatty acids. Sucrose fatty acid esters were completely degraded to sucrose and fatty acids in alkaline ethanol overnight at 25°C. Sucrose was derivatized with pyridine, trimethylchlorosilane, and N-trimethylsilylimidazole and the sucrose TMS derivative was determined on a 2% OV-17 column. Fatty acids were extracted with ethyl ether, methylated with BF3-methanol complex at 65°C, and determined on a 2% DEGS + 0.5% H3PO4 column. This method was applied to selected sucrose fatty acid esters. For example, sucrose and fatty acids derived from 50 mg sample F20 were 10.6-11.0 and 38.1-39.0 mg, respectively. Total amounts were 48.7-50.0 mg with a standard deviation of 0.4 (n = 6).

scholarly journals Analytical Methods for the Determination of Fatty Acid Esters of Hydroxy Fatty Acids (FAHFAs) in Biological Samples, Plants and Foods

Biomolecules ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1092 ◽  
Author(s):  
Maroula G. Kokotou
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Biological Samples ◽  
Analytical Methods ◽  
Biological Significance ◽  
Hydroxy Fatty Acids ◽  
Fatty Acid Esters ◽  
Lipid Species ◽  
Acid Esters

Fatty acid esters of hydroxy fatty acids (FAHFAs) constitute a class of recently identified novel lipids exhibiting anti-diabetic and anti-inflammatory effects. Due to their high biological significance, a tremendous effort has been devoted to the development of analytical methods for the detection and quantitation of FAHFAs during the last five years. The analysis of FAHFAs is very challenging due to the great number of possible regio-isomers arising from the great number of possible combinations of FAs with HFAs, and the low abundancies of FAHFAs in biological samples. The aim of this review article is to summarize all the cutting-edge analytical methodologies for the determination of FAHFAs in biological samples, plant tissues and food matrices, with emphasis on extraction and analysis steps. All the analytical methodologies rely on the use of liquid chromatography–mass spectrometry (LC-MS), providing high sensitivity due to the MS detection. Powerful and robust analytical methodologies may highly contribute in studying FAHFAs levels under various biomedical conditions, and facilitate our understanding of the role of these lipid species in physiological and pathological conditions.

scholarly journals AIG1 and ADTRP are endogenous hydrolases of fatty acid esters of hydroxy fatty acids (FAHFAs) in mice

2020 ◽  
Vol 295 (18) ◽  
pp. 5891-5905 ◽  
Author(s):  
Meric Erikci Ertunc ◽  
Bernard P. Kok ◽  
William H. Parsons ◽  
Justin G. Wang ◽  
Dan Tan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Protein Profiling ◽  
Hydroxy Fatty Acids ◽  
Fatty Acid Esters ◽  
Genetic Studies ◽  
Health And Disease ◽  
Acid Esters

Fatty acid esters of hydroxy fatty acids (FAHFAs) are a newly discovered class of signaling lipids with anti-inflammatory and anti-diabetic properties. However, the endogenous regulation of FAHFAs remains a pressing but unanswered question. Here, using MS-based FAHFA hydrolysis assays, LC-MS–based lipidomics analyses, and activity-based protein profiling, we found that androgen-induced gene 1 (AIG1) and androgen-dependent TFPI-regulating protein (ADTRP), two threonine hydrolases, control FAHFA levels in vivo in both genetic and pharmacologic mouse models. Tissues from mice lacking ADTRP (Adtrp-KO), or both AIG1 and ADTRP (DKO) had higher concentrations of FAHFAs particularly isomers with the ester bond at the 9th carbon due to decreased FAHFA hydrolysis activity. The levels of other lipid classes were unaltered indicating that AIG1 and ADTRP specifically hydrolyze FAHFAs. Complementing these genetic studies, we also identified a dual AIG1/ADTRP inhibitor, ABD-110207, which is active in vivo. Acute treatment of WT mice with ABD-110207 resulted in elevated FAHFA levels, further supporting the notion that AIG1 and ADTRP activity control endogenous FAHFA levels. However, loss of AIG1/ADTRP did not mimic the changes associated with pharmacologically administered FAHFAs on extent of upregulation of FAHFA levels, glucose tolerance, or insulin sensitivity in mice, indicating that therapeutic strategies should weigh more on FAHFA administration. Together, these findings identify AIG1 and ADTRP as the first endogenous FAHFA hydrolases identified and provide critical genetic and chemical tools for further characterization of these enzymes and endogenous FAHFAs to unravel their physiological functions and roles in health and disease.

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