Use of 3-Methyl-2-Benzothiazolone Hydrazone in an Automated Colorimetric Method for Determination of Triglycerides in Plasma or Serum

1972 ◽  
Vol 18 (11) ◽  
pp. 1350-1354 ◽  
Author(s):  
W E Neeley ◽  
G E Goldman ◽  
C A Cupas
Keyword(s):  
Colorimetric Method ◽  
High Sensitivity ◽  
Low Flow ◽  
Activated Alumina ◽  
Sodium Metaperiodate ◽  
Plasma Triglycerides ◽  
Incubation Periods ◽  
On Line ◽  
Cationic Adduct

Abstract A new automated colorimetric method is described for the determination of serum or plasma triglycerides. The samples are extracted with 2-propanol containing activated alumina. An AutoAnalyzer Sampler II and Proportioning Pump II are used in combination with a Gilford Model 300 N spectrophotometer and Model 3091-A debubbler flow cell. Following on-line saponification, glycerol is oxidatively cleaved with sodium metaperiodate. The resulting formaldehyde is reacted with 3-methyl-2-benzothiazolone hydrazone (MBTH) under mild conditions to produce the corresponding azine. Further reaction of MBTH with the azine in the presence of an oxidizing agent results in the formation of a blue cationic adduct. Beer's law is obeyed, at 670 nm, to a triglyceride concentration of 400 mg/100 ml. Because of the high sensitivity of the MBTH reaction, short incubation periods, high sampling rates (60/h), and low flow rates can be used. The results correlate well with those obtained by the Kessler-Lederer method and by a totally enzymatic method.

Liquid Chromatographic Determination of Zoalene in Medicated Feeds

1984 ◽  
Vol 67 (5) ◽  
pp. 861-862 ◽  
Author(s):  
John Morawski ◽  
Glenn Kyle
Keyword(s):  
Colorimetric Method ◽  
Chromatographic Determination ◽  
Activated Alumina ◽  
Reverse Phase ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Aoac Official ◽  
Liquid Chromatographic ◽  
Medicated Feeds

Abstract A rapid, reliable separation and quantitation of zoalene (3,5-dinitroo-toluamide) from feeds is accomplished by using reverse phase liquid chromatography (LC) and ultraviolet detection. An extraction technique which is similar to the present AOAC official colorimetric method is used before chromatographic analysis. This extraction is followed by an activated alumina cleanup and LC to separate zoalene from feed matrix. The methodology was applied to a variety of spiked feed matrices, and yielded good recoveries. Liquid chromatographic results were shown to correlate with colorimetric determinations.

Continuous On-Line Determination of Recirculation by Thermodilution in Hemodialysis Patients

1995 ◽  
Vol 18 (9) ◽  
pp. 526-529 ◽  
Author(s):  
G. Bonforte ◽  
B. Dozio ◽  
R. Scanziani ◽  
G. Crema ◽  
M. Surian
Keyword(s):  
Blood Pressure ◽  
Blood Flow ◽  
Hemodialysis Patients ◽  
Low Flow ◽  
Blood Temperature ◽  
Thermodilution Method ◽  
On Line ◽  
Temperature Monitor ◽  
Sample Method

The Consistency Of The Determination Of A-V Fistula Recirculation (R) Using The Thermodilution Method (T) With A New Probe (Blood Temperature Monitor, Btm Fresenius A.G.) Was Studied In 32 Patients (Avf: Proximal 34%, Distal 63%, Graft 3%). We Compared R Calculated By T With Both The Traditional Three-Sample Method (C) And The Low-Flow Three-Sample Method (L); Both Bun And Creatinine (Cr) Were Measured In All Samples At The Beginning And At The End Of The Session. T Was Also Determined At The 2Nd And 3Rd Hour. There Was A Significant Correlation Between T And Either C Or L At The Start Of The Session (Bun And Cr) As Well As At The End (Only Cr). R Was Higher (11.9±10) In Proximal Avf Than In The Distal (5+3.1%; P0.01) When Measured By T At The Same Blood Flow (Qb: 313±45 Vs 343+52 Mls/Min, P=Ns). T Increased But Not Significantly By Increasing Qb From 150 To 300 Mls/Min In Ten Patients. No Correlation Was Found During The Session Between Blood Pressure And T Variations. In Conclusion, T And L Give Very Similar Results While C Overestimates Recirculation. R Is Easy To Perform Repeteadly By T With Results Available Online.

A High Sensitivity Digital Raman Difference Spectrometer for Studies on Solutions of Biological Molecules with On-Line Computer Control of Data Acquisition and Reduction

1976 ◽  
Vol 30 (2) ◽  
pp. 168-179 ◽  
Author(s):  
Ray W. Chrisman ◽  
Jim C. English ◽  
R. Stuart Tobias
Keyword(s):  
Aqueous Solutions ◽  
Computer Control ◽  
Visible Spectrum ◽  
High Sensitivity ◽  
Biological Molecules ◽  
Difference Spectra ◽  
On Line ◽  
Dilute Aqueous Solutions ◽  
Good Signal

The design and construction of a computer-controlled Raman spectrophotometer for the determination of difference spectra among up to four samples is described. The advantages of control by a minicomputer compared to a microcomputer or a hard wired controller are discussed. Conditions for the attainment of good signal/noise ratios with aqueous solutions of nucleotides in the 1 to 25 m M range are examined for the case of excitation in the visible spectrum to minimize radiation damage. Applications to studies of dilute aqueous solutions of biological molecules are discussed. The interactions of small molecules with bio-polymers, particularly, are examined.

Optimized liquid-chromatographic method for fluorometric determination of urinary delta-aminolevulinic acid in workers exposed to lead.

1987 ◽  
Vol 33 (9) ◽  
pp. 1665-1667 ◽  
Author(s):  
K Tomokuni ◽  
M Ichiba ◽  
Y Hirai ◽  
T Hasegawa
Keyword(s):  
Present Method ◽  
High Performance ◽  
Aminolevulinic Acid ◽  
Colorimetric Method ◽  
High Sensitivity ◽  
Liquid Chromatograph ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Urinary Ala

Abstract We developed a fluorometric method for determining delta-aminolevulinic acid (ALA) in urine of lead workers. A high-performance liquid chromatograph (HPLC) equipped with a fluorescence HPLC monitor is used. The detection limit for aqueous ALA is 20 micrograms/L (15 pmol of ALA in the 100-microL sample). The working linear range of urinary ALA concentration was 0.1 to 100 mg/L. In 25 lead-exposed workers, ALA values by the present method for urine correlated well with those obtained by a conventional colorimetric method (r = 0.996). The advantage of the present method for micro-determination of urinary ALA is its high sensitivity.

scholarly journals Carbon Quantum Dots Prepared with Chitosan for Synthesis of CQDs/AuNPs for Iodine Ions Detection

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 1043 ◽  
Author(s):  
Juanjuan Song ◽  
Li Zhao ◽  
Yesheng Wang ◽  
Yun Xue ◽  
Yujia Deng ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Color Change ◽  
Colorimetric Method ◽  
High Sensitivity ◽  
Hydrothermal Carbonization ◽  
Carbon Quantum Dots ◽  
Water Soluble ◽  
Synthetic Process ◽  
Iodide Ions

Water-soluble and reductive carbon quantum dots (CQDs) were fabricated by the hydrothermal carbonization of chitosan. Acting as a reducing agent and stabilizer, the as-prepared CQDs were further used to synthesize gold nanoparticles (AuNPs). This synthetic process was carried out in aqueous solution, which was absolutely “green”. Furthermore, the CQDs/AuNPs composite was used to detect iodine ions by the colorimetric method. A color change from pink to colorless was observed with the constant addition of I− ions, accompanied by a decrease in the absorbance of the CQDs/AuNPs composite. According to the absorbance change, a favorable linear relationship was obtained between ΔA and I− concentration in the range of 20–140 μM and 140–400 μM. The detection limit of iodide ions, depending on the 3δ/slope, was estimated to be 2.3 μM, indicating high sensitivity to the determination of iodide. More importantly, it also showed good selectivity toward I− over other anion ions, and was used for the analysis of salt samples. Moreover, TEM results indicated that I− ions induced the aggregation of CQDs/AuNPs, resulting in changes in color and absorbance.

Sign in / Sign up

Export Citation Format

Share Document