Simplified Primary Screening Procedure for Detection of Hyperlipidemias in Healthy Individuals

1972 ◽  
Vol 18 (12) ◽  
pp. 1463-1467 ◽  
Author(s):  
Gerald S Berenson ◽  
S R Srinivasan ◽  
P S Pargaonkar ◽  
B Radhakrishnamurthy ◽  
Edward R Dalferes
Keyword(s):  
Present Method ◽  
Analytical Ultracentrifugation ◽  
Healthy Adults ◽  
Screening Procedure ◽  
Diabetic Patients ◽  
Healthy Individuals ◽  
Primary Screening ◽  
Adults And Children

Abstract A simplified primary screening procedure is described for determining serum β- plus pre-β-lipoproteins, which is more rapid and less expensive than cholesterol or triglyceride determinations. This method is based on the ability of β- and pre-β-lipoproteins to form insoluble complexes with heparin in presence of Ca2+. Comparison with analytical ultracentrifugation indicated the accuracy of concentrations determined by the present method. This method was used to screen a group of healthy adults and children and diabetic patients. The results show that measuring serum β- plus pre-β-lipoprotein concentrations may be more useful for screening and detecting subtle abnormalities than is determination of either cholesterol or triglyceride.

Determination of D-sorbitol in human erythrocytes by an improved enzymatic method with fluorometric detection.

1988 ◽  
Vol 34 (11) ◽  
pp. 2327-2330 ◽  
Author(s):  
J C Liao ◽  
M Rountree ◽  
R Good ◽  
J Hook ◽  
C Punko
Keyword(s):  
Isotonic Saline ◽  
Human Erythrocytes ◽  
Sorbitol Dehydrogenase ◽  
Diabetic Patients ◽  
Fluorometric Detection ◽  
Enzymatic Method ◽  
Healthy Individuals ◽  
Hemoglobin Content ◽  
Hclo4 Solution

Abstract In this enzymatic method to analyze erythrocytes for D-sorbitol, the erythrocytes were separated from plasma by centrifugation, washed with isotonic saline (9 g/L NaCl), then diluted threefold with more saline. We lysed 1.5 mL of the diluted erythrocytes with chloroform and precipitated the protein with 58 g/L HClO4 solution. The resulting supernates were mixed with buffer, NAD+, and sorbitol dehydrogenase (EC 1.1.1.14). For standards, we added sorbitol to the erythrocytes before lysing. After 25 min of incubation at 37 degrees C, the fluorescence responses were recorded. Results for sorbitol were corrected for sample-blank and enzyme fluorescence, and were then normalized for hemoglobin content. Response was linear for a sorbitol concentration range of 1 to 30 mumol/L. Reproducibility was good, with an average CV of 2.5% at 10 mumol/L. Results for healthy individuals and diabetic patients are presented.

scholarly journals The circulating level of interleukins 6 and 18 in ischemic and idiopathic dilated cardiomyopathy

2019 ◽  
Vol 11 (2) ◽  
pp. 132-137
Author(s):  
Mahdiyar Iravani Saadi ◽  
Mohammad Ali Babaee Beigi ◽  
Maryam Ghavipishe ◽  
Maryam Tahamtan ◽  
Bita Geramizadeh ◽  
...  
Keyword(s):  
Heart Failure ◽  
Dilated Cardiomyopathy ◽  
Left Ventricular ◽  
Expression Level ◽  
Idiopathic Dilated Cardiomyopathy ◽  
Left Ventricular Ejection ◽  
Diabetic Patients ◽  
Healthy Individuals ◽  
P Values

Introduction: By aging population, the heart failure and its life-threatening complications have become an enormous issue in public health. Regarding the inflammation as a major contributing pathological factor, the determination of most important inflammatory targets for immunomodulation is a problematic puzzle in the treatment of heart failure patients and the inflammatory pathways primarily involved in different underlying conditions contributing to heart failure can be an area which is worthy of focused research. Considering the dilated cardiomyopathy (DCM) as a relatively high-incident disease leading to heart failure, the aim of this study is to determine the difference in the expression level of interleukin (IL)-6 and IL-18 in patients with ischemic and idiopathic DCM. Methods: 39 non-diabetic patients with ischemic and 37 ones with idiopathic DCM were enrolled in the study. 48 healthy individuals were also considered as control group. For quantitative determination of the mRNA expression level of IL-6 and IL-18 genes, an in-house- SYBR Green real-time PCR was used and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was considered as internal control gene. The left ventricular end-diastolic volume (LVEDV) and left ventricular ejection fraction (LVEF) was calculated by 2D echocardiographic assessment. Data were finally analyzed via SPSS statistical software version 19.0 using independent t test and 2-∆∆Ct method and P<0.05 were considered statistically significant. Results: The IL-6 was significantly higher expressed in patients with ischemic and idiopathic DCM than in healthy controls (274.3 and 168.8 times, respectively, both P values <0.001). The same higher expression of IL-18 was observed in ischemic DCM (48.5 times) and idiopathic DCM (45.2 times) compared with healthy individuals (both P values <0.001). Conclusion: Both ischemic and idiopathic DCM associates with IL-6 and IL-18 overexpression. However, no significant difference was observed between these two subtypes of DCM in either interleukin expression level. There is certainly need to further studies for evaluating the uniformity of results and also assessing other molecules in determining their roles in pathophysiology and probable utility for management.

Determination of Plasminogen in Human Plasma by the Lysis Time Method

1966 ◽  
Vol 16 (01/02) ◽  
pp. 001-017 ◽  
Author(s):  
W Berg ◽  
K Korsan-Bengtsen ◽  
J Ygge
Keyword(s):  
Human Plasma ◽  
Present Method ◽  
Blood Donors ◽  
Fibrinogen Concentration ◽  
Plasmin Activity ◽  
Time System ◽  
Lysis Time ◽  
High Dilutions ◽  
Single Determination

SummaryA one-stage lysis time system containing fibrinogen, streptokinase, thrombin, and a known, small amount of plasminogen was used to determine plasminogen in plasma.The known amount of plasminogen was added to the system in order to keep the lysis times relatively short when a highly diluted plasma was tested. High dilutions of plasma were used to reduce the influence of the plasma inhibitors.The calculation of the plasminogen concentration was made on the basis of the correlation: “plasminogen = fibrinogen/lysis time” which was valid in the system. The method allowed determination of plasminogen in plasma with varying fibrinogen concentrations, as the fibrinogen concentration in plasma was considered in the calculation.The presence of “spontaneous” plasmin activity in the plasma did not influence the plasminogen determination. Estimated by this method, the plasminogen content in plasma from 32 blood donors aged 25-45 years was 13.1 ±2.4 casein u/ml. The error of a single determination was 0.3 casein u/ml. The plasminogen content in plasma, determined with the present method, is about 3-4 times higher than the content found when a caseinolytic method is used.

scholarly journals Alanine Aminopeptidase, γ-Glutamyl Transferase and β2-microglobulin as Diagnostic Markers in Patients with Rheumatoid Arthritis

2008 ◽  
Vol 27 (1) ◽  
pp. 59-63
Author(s):  
Dejan Spasovski ◽  
Todor Gruev ◽  
Nada Marina ◽  
Jordan Calovski ◽  
Ljubinka Rajčevska ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Diagnostic Markers ◽  
Border Region ◽  
Healthy Individuals ◽  
Kinetic Assay ◽  
Renal Lesions ◽  
Alanine Aminopeptidase ◽  
Glutamyl Transferase ◽  
Higher Sensitivity

Alanine Aminopeptidase, γ-Glutamyl Transferase and β2-microglobulin as Diagnostic Markers in Patients with Rheumatoid Arthritis The purpose of this research is to evaluate the values of alanine aminopeptidase (AAP), γ-glutamyl transferase (γ-GT), and β2-Microglobulin in urine (β2-M), in untreated rheumatoid arthritis (RA) and to define the effect of untreated rheumatoid arthritis on the tubular function and brush border region. We used a kinetic assay for AAP, standard methods by the IFCC for γ-glutamyl transferase and MEIA for the determination of β2-Microglobulin in urine in 70 participants (35 untreated RA patients, 35 healthy individuals). From the total of 35 RA patients, 24 patients had AAP (sensitivity of the test 68.57%), 16 patients had γ-GT enzymuria (sensitivity of the test 45.71%), while the presence of β2-Microglobulin in urine was found in a very low percentage. Out of 18 RF negative patients, 14 patients are AAP positive, 10 patients were γ-GT positive, while the presence of β2-Microglobulin in urine was not detected. Among 17 RF positive RA patients, the presence of AAP was noticed in 10, the presence of γ-GT in 6 patients, while the presence of β2-Microglobulin in urine was not detected. AAP has higher sensitivity than γ-GT and β2-Microglobulin in the detection of asymptomatic renal lesions in untreated RA.

Is there a relation between serum methylarginine levels and infertility?

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Yasin Saygın ◽  
Abdullah Sivrikaya ◽  
Turan Akdağ ◽  
Duygu Dursunoğlu ◽  
Mehmet Kaynar ◽  
...  
Keyword(s):  
Sperm Concentration ◽  
Reproductive Period ◽  
Healthy Individuals ◽  
Adma Level ◽  
Chromatography Tandem Mass Spectrometry ◽  
Potential Biomarker ◽  
Liquid Chromatography Tandem Mass ◽  
Arginine Residues ◽  
Dimethyl Arginine

Abstract Objectives Infertility is defined as the absence of pregnancy within the reproductive period despite regular sexual intercourse. Methylarginines are formed as a result of methylation of arginine residues in proteins and formed in three forms as asymmetric dimethyl arginine (ADMA), symmetrical dimethyl arginine (SDMA) and monomethylarginine (L-NMMA). So, here, we aimed to evaluate arginine and their derivatives levels in fertile and infertile individuals. Methods Present study were consist of 30 oligozoospermia patients (proven by spermiogram analysis) and 30 healthy individuals with normozoospermia group who were applied to the urology department. With blood samples taken from individuals, serum methylarginine and its derivatives levels were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Clinic data and demographic characteristics of individuals were also recorded at the same time. Results The serum ADMA level (0.38 ± 0.07) of the oligozoospermia group was found to be significantly higher than the normozoospermia group (0.35 ± 0.05) (p=0.046). A positive correlation were observed between ADMA and SDMA (r=0.686, p=0.000), HArg and SDMA (r=0.611, p=0.001), citrulline and L-NMMA (r=0.595, p=0.001) in patients with oligosospermia. The increase in SDMA, arginine and HArg levels and a decrease in L-NMMA and citrulline levels were not significant as statistically. Also, the ADMA level was found to be high in individuals with low sperm concentration. Conclusions Consequently, serum ADMA levels of individuals with oligozoospermia were statistically significantly higher than those with normozoospermia. As proposal, determination of ADMA levels may be a potential biomarker parameter in terms of early diagnosis of fertility and infertility.

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