Biological Activity of a Semisynthetic Flavonoid, O-(β-Hydroxyethyl)rutoside: Light-Scattering and Metabolic Studies of Human Red Cells and Platelets

1973 ◽  
Vol 19 (1) ◽  
pp. 31-35 ◽  
Author(s):  
J W ten Cate ◽  
N J van Haeringen ◽  
J Gerritsen ◽  
E Glasius
Keyword(s):  
Biological Activity ◽  
Light Scattering ◽  
Platelet Aggregation ◽  
Cell Aggregation ◽  
Red Cells ◽  
Red Cell ◽  
High Concentrations ◽  
Platelet Functions ◽  
Human Red Cells ◽  
Red Cell Aggregation

Abstract The effect of O-(β-hydroxyethyl)-rutoside (HR) on human erythrocyte and platelet functions is reported. Only high concentrations of the compound distinctly inhibited red cell and platelet aggregation induced by ADP and epinephrine. Lower concentrations of HR inhibit [14C8]adenosine incorporation into red cells as well as into platelets. Inhibition occurs at both 0°C and 37°C, presumably because diffusion of [14C8]adenosine is hindered. Phosphorylation of [14C8] adenosine by the platelets is not inhibited by HR. The inhibition of red cell aggregation is reversed by washing the cells with plasma. Collectively, these findings indicate an effect of the compound at the site of the membrane, independent of cellular metabolism

Microscopy and viscometry of blood flowing under uniform shear rate(rheoscopy)

1969 ◽  
Vol 26 (5) ◽  
pp. 674-678 ◽  
Author(s):  
Holger Schmid-Schoenbein ◽  
Roe Wells ◽  
Robert Schildkraut
Keyword(s):  
Platelet Aggregation ◽  
Blood Rheology ◽  
Cell Aggregation ◽  
Sine Wave ◽  
Dark Field ◽  
Red Cell ◽  
Shear Rates ◽  
Dark Field Illumination ◽  
Sine Wave Generator ◽  
Red Cell Aggregation

Transparent cone and plate in a Wells-Brookfield LVT viscometer allow direct microscopic observation ( x 60–400 magnification) of blood flowing under specific rates of shear (0.6–1,380 set–1) while simultaneously measuring shear stress. The viscometer is placed on the stage of an inverted microscope; observation in bright-field and dark-field illumination is made through a thin optical quartz inserted in the plate. The range of shear rates is extended by the use of different cone angles and can be adapted to any desired level by driving the viscometer motor with a sine-wave generator with variable output. The use of the instrument confirmed recent theories about the role of reversible red cell aggregation upon the rheology of blood at low shear rates. The influence of quantified flow forces on reversible red cell aggregation and irreversible platelet aggregation can be examined directly. blood rheology; microscopy of blood flow; platelet aggregation; red cell aggregation Submitted on August 5, 1968

Effects of Halothane on Platelet Function

1980 ◽  
Vol 44 (03) ◽  
pp. 143-145 ◽  
Author(s):  
J Dalsgaard-Nielsen ◽  
J Gormsen
Keyword(s):  
Platelet Aggregation ◽  
Platelet Function ◽  
Platelet Rich Plasma ◽  
Human Platelets ◽  
Halothane Anaesthesia ◽  
Inhibition Of Platelet Aggregation ◽  
Transient Impairment ◽  
High Concentrations ◽  
Uptake And Release ◽  
Platelet Functions

SummaryHuman platelets in platelet rich plasma (PRP) incubated at 37° C with 0.3–2% halothane for 5–10 min lost the ability to aggregate with ADP, epinephrine and collagen.At the same time uptake and release of 14C-serotonin was inhibited. When halothane supply was removed, platelet functions rapidly returned to normal. However, after high concentrations of halothane, the inhibition of platelet aggregation was irreversible or only partially reversible.The results suggest that halothane anaesthesia produces a transient impairment of platelet function.

Red Cell Aggregation Induced by a High Molecular Weight Gelatin Plasma Substitute

1971 ◽  
Vol 3 (6) ◽  
pp. 428-435 ◽  
Author(s):  
P.M. Scholz ◽  
J. Engeset ◽  
N.A. Matheson ◽  
U.F. Gruber
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Cell Aggregation ◽  
Red Cell ◽  
Plasma Substitute ◽  
Red Cell Aggregation

scholarly journals Kinetics and stoichiometry of Na-dependent Li transport in human red blood cells.

1978 ◽  
Vol 72 (2) ◽  
pp. 249-265 ◽  
Author(s):  
B Sarkadi ◽  
J K Alifimoff ◽  
R B Gunn ◽  
D C Tosteson
Keyword(s):  
Transport System ◽  
Red Cells ◽  
Normal Male ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Human Red Blood Cells ◽  
Na Efflux ◽  
Tightly Coupled ◽  
Male Subjects ◽  
Human Red Cells

This paper describes the kinetics and stoichiometry of a tightly coupled Na-Li exchange transport system in human red cells. The system is inhibited by phloretin and furosemide but not by ouabain. Li influx by this system increases and saturates with increasing concentrations of external Li and internal Na and is inhibited competitively by external Na. Comparable functions relate Li efflux and Na efflux to internal and external Li and Na concentrations. Analysis of these relations yields the following values for the ion concentrations required to half-maximally activate the transport system: internal Na and Li 9.0 and 0.5 mM, respectively, external Na and Li 25 and 1.5 mM, respectively. The system performs a 1:1 exchange of Na and Li moving in opposite directions across the red cell membrane. We found no evidence for a simultaneous transport of more than one Na and Li by the system. The maximum transport rate of Na-dependent Li transport varied between 0.1 and 0.37 mmol/(liter of cells X h) in the red cells of the five normal male subjects studied. No significant variations between individual subjects were observed for bicarbonate-stimulated Li transport and for the residual Li fluxes which occur in the absence of bicarbonate and in the presence of ouabain plus phloretin.

scholarly journals Nonspecific red cell aggregation interferes in the interpretation of gel test results

2012 ◽  
Vol 6 (1) ◽  
pp. 44
Author(s):  
Sabita Basu ◽  
Ravneet Kaur ◽  
Gagandeep Kaur ◽  
Paramjit Kaur ◽  
Anita Tahlan
Keyword(s):  
Cell Aggregation ◽  
Red Cell ◽  
Test Results ◽  
Red Cell Aggregation

Increased Red Cell Aggregation in Diabetes Mellitus: Association with Cardiovascular Risk Factors

1993 ◽  
Vol 10 (1) ◽  
pp. 21-26 ◽  
Author(s):  
S. MacRury ◽  
S.E. Lennie ◽  
P. McColl ◽  
R. Balendra ◽  
A.C. MacCuish ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Cardiovascular Risk ◽  
Cardiovascular Risk Factors ◽  
Cell Aggregation ◽  
Red Cell ◽  
Red Cell Aggregation

Acetylcholinesterase in Human Erythroid Cells

1973 ◽  
Vol 12 (3) ◽  
pp. 911-923
Author(s):  
R. J. SKAER
Keyword(s):  
Endoplasmic Reticulum ◽  
Nervous System ◽  
Golgi Apparatus ◽  
Red Cells ◽  
The Other ◽  
Red Cell ◽  
Erythroid Cells ◽  
Cell Replacement ◽  
Kinetics Of ◽  
Human Red Cells

Acetylcholinesterase is present in human red cells but cannot be demonstrated by the copper thiocholine test. The enzyme is revealed, however, in the perinuclear cisterna, endoplasmic reticulum and Golgi apparatus of red cell precursors. It is suggested that 2 forms of the enzyme are present, one of which can be demonstrated by the copper thiocholine test, the other cannot; one form may be the precursor of the other. These observations may cast light on the kinetics of red cell replacement and on the interpretation of the results from the copper thiocholine test on other tissues such as the nervous system.

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