Gas Chromatographic/Thin-Layer Chromatographic Analysis of Acetylated Codeine and Morphine in Urine

1975 ◽  
Vol 21 (10) ◽  
pp. 1486-1489 ◽  
Author(s):  
Naresh C Jain ◽  
Thomas C Sneath ◽  
Robert D Budd ◽  
Wai J Leung
Keyword(s):  
Gas Chromatography ◽  
Organic Solvent ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Chromatographic Analysis ◽  
Ammonium Hydroxide ◽  
Thin Layer Chromatographic Analysis ◽  
Layer Chromatography ◽  
Thin Layer Chromatography System

Abstract This procedure positively identifies codeine and morphine in urine. Urine samples are hydrolyzed and extracted with organic solvent, and the extracts are evaporated and acetylated. The presence of codeine and morphine is ascertained by gas chromatography (3% OV-25 and 3% Poly-A 103 columns) and confirmed by thin-layer chromatography (system: ethyl acetate/acetone/concd ammonium hydroxide, 100/10/4.5 by vol; reagent: iodoplatinate). As little as 0.5 mg each of codeine and morphine per liter, in free and conjugated forms, is detectable by this method.

GAS CHROMATOGRAPHIC ANALYSIS OF THE UNCONJUGATED, NEUTRAL NON-POLAR STEROIDS OF HUMAN PLACENTA

1968 ◽  
Vol 40 (1) ◽  
pp. 91-100 ◽  
Author(s):  
J. CHAMBERLAIN ◽  
N. F. MORRIS ◽  
NANCY C. SMITH
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chromatographic Analysis ◽  
Stationary Phases ◽  
Preliminary Examination ◽  
Normal Human ◽  
Acetate Formation ◽  
Chromatographic Peaks ◽  
Layer Chromatography

SUMMARY Normal human term placentae have been examined for neutral non-polar steroids by gas chromatography. Preliminary examination of a number of neutral extracts showed a consistent pattern of chromatographic peaks on both non-selective (SE-30) and selective (QF-1) stationary phases. The extracts were further fractionated by thin-layer chromatography and reexamined in detail by acetate formation, adsorption and partition thin-layer chromatography, and gas chromatography. The major steroids identified were progesterone, 3β-hydroxy-pregn-5-en-20-one, 20α-hydroxy-pregn-4-en-3-one and 3β-hydroxy-5α-pregnan-20-one; minor amounts of 3α-hydroxy-5β-pregnan-20-one and 5β-pregnane-3α,20α-diol were also identified.

Differentiation of Geographic Origin of Spices. II. Oregano by Gas Chromatography and Thin Layer Chromatography

1969 ◽  
Vol 52 (6) ◽  
pp. 1184-1189
Author(s):  
William H Stahl Stahl ◽  
Jean N Skarzynski ◽  
William A Voelker
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Geographic Origin ◽  
Chromatographic Technique ◽  
Two Dimensional ◽  
Second Development ◽  
Chromatographic Procedure ◽  
Layer Chromatography

Abstract Both a gas chromatographic and a thin layer chromatographic procedure have been devised to differentiate the origin of oreganos. The gas chromatographic procedure utilizes the observation that the ratio of the concentration of carvacrol to thymol in Greek oregano is approximately 7:1, whereas in Mexican oregano it is 1:1. The decision can be reinforced by a fluorescence thin layer chromatographic technique, using a hexane-ethyl acetate-tetrahydrofuran mixture as the developing solvent. A two-dimensional TLC, using acetone-ethermethylene chloride for the second development, affords a clean-cut separation of all spots and is particularly useful in the diagnosis of mixtures.

Direct Fluorescent Detection of Organothiophosphorus Pesticides and Some of Their Sulfur-containing Breakdown Products After Thin Layer Chromatography

1967 ◽  
Vol 50 (5) ◽  
pp. 1088-1098
Author(s):  
Mohamed Tawfik H Ragab
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Ultraviolet Light ◽  
Congo Red ◽  
Fluorescent Detection ◽  
Bromine Vapor ◽  
Layer Chromatography ◽  
Sulfur Containing ◽  
Dark Blue

Abstract A rapid, simple, convenient, and widely applicable method for the direct fluorescent detection of organothiophosphorus pesticides and some postulated breakdown products of these compounds is presented. The coinpounds were spotted on thin layer chromatographic sheets, developed in ethyl acetate :nhexane, and made visible by exposure to bromine vapor followed by spraying with ferric chloride and 2-(o-hydroxy phenyl) benzoxazole. Of the 47 compounds tested, 32 compounds produced fluorescent blue spots vinder longwave ultraviolet light; these consisted of 25 organothiophosphorus pesticides, 5 sulfur-containing breakdown products, and phosphoric and hypophosphorous acids. A superimposed Congo red spray destroyed the fluorescence and resulted in dark blue spots against a red backgrovind. The sensitivity of this method is in the range of 0.2 to 5.0 μg, depending on the specific compound.

Thin-layer chromatography and gas chromatography-mass spectrometry examination of shoe materials from patients with shoe dermatitis

2015 ◽  
Vol 72 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Sri A. Febriana ◽  
Erik Zimerson ◽  
Cecilia Svedman ◽  
Winarto Haryadi ◽  
Pieter-Jan Coenraads ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Layer Chromatography
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