Glycosylated hemoglobin measured by affinity chromatography: micro-sample collection and room-temperature storage.

1983 ◽  
Vol 29 (6) ◽  
pp. 1080-1082 ◽  
Author(s):  
R R Little ◽  
J D England ◽  
H M Wiedmeyer ◽  
D E Goldstein
Keyword(s):  
Affinity Chromatography ◽  
Whole Blood ◽  
High Performance ◽  
Room Temperature ◽  
Hemoglobin A1c ◽  
Glycosylated Hemoglobin ◽  
Exchange Chromatography ◽  
Sample Collection ◽  
Room Temperature Storage ◽  
Temperature Storage

Abstract Under proper conditions, whole blood can be stored at room temperature for as long as 21 days before measurement of glycosylated hemoglobin by affinity chromatography. Whole blood (anticoagulated with EDTA or heparin) was placed in capillary tubes, which were then sealed at both ends and stored at room temperature. Just before assay, whole blood was rinsed from the tubes and diluted 10-fold with water. Samples of each patient's blood were assayed as whole-blood hemolysates by affinity chromatography after zero, seven, 14, and 21 days of storage. Values for glycosylated hemoglobin did not change over 21 days of storage and values for each storage day correlated well (r = 0.97, p less than .0001) with hemoglobin A1C measured in fresh erythrocyte hemolysates by "high-performance" liquid ion-exchange chromatography.

Effects of whole blood storage on results for glycosylated hemoglobin as measured by ion-exchange chromatography, affinity chromatography, and colorimetry.

1983 ◽  
Vol 29 (6) ◽  
pp. 1113-1115 ◽  
Author(s):  
R R Little ◽  
J D England ◽  
H M Wiedmeyer ◽  
D E Goldstein
Keyword(s):  
Ion Exchange ◽  
Affinity Chromatography ◽  
Whole Blood ◽  
High Performance ◽  
Glycosylated Hemoglobin ◽  
Thiobarbituric Acid ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Sample Collection ◽  
Sample Stability

Abstract After storage of whole blood at either 4 or 20 degrees C, results for glycosylated hemoglobin by ion-exchange chromatography ("high-performance" liquid and mini-column chromatography), thiobarbituric acid colorimetry, and affinity chromatography were compared. At 4 degrees C, all methods gave acceptable results for samples stored for as long as a week. At 20 degrees C, the colorimetric and affinity methods also showed sample stability for a week or more. The ion-exchange methods were associated with a marked increase in values for glycosylated hemoglobin after a few days of storage. Evidently, care in details of sample collection and handling is especially important for ion-exchange methods, and the colorimetric and affinity methods have advantages over ion exchange in situations where long delays between sample collection and assay are unavoidable.

scholarly journals Assay Of Paracetamol Syrup In Different Storage Temperatures By High Performance Liquid Chromatography

2019 ◽  
Vol 10 (1) ◽  
pp. 72-80
Author(s):  
Dian Maria Ulfa ◽  
Dodi Irawan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Room Temperature ◽  
Hplc Method ◽  
Room Temperature Storage ◽  
Phase Water ◽  
Storage Temperatures ◽  
Temperature Storage

Paracetamol formulated in solution, can be hydrolyzed to p-aminophenol, then can be broken down again into quinone-imin. The decomposition rate of paracetamol increases when the temperature is raised and if it is exposed to light directly. The habit of people in storing drugs after the packaging is opened and will be used again raises concerns about the quality of the drugs contained in the drug. The purpose of this study was to determine the level of paracetamol stored at room temperature and chiller temperature using the High Performance Liquid Chromatography (HPLC) method. The study was conducted by testing the levels of paracetamol syrup on the first day and after being stored for three days. Samples were divided into two groups of storage temperatures, room temperature and chiller temperature. The HPLC system used is the L1 column, the mobile phase water-methanol P mixture (3: 1), the flow rate 1.5. The average paracetamol levels at day 1 storage at room temperature and chiller temperature were 104.61% and 102.38%. The average paracetamol levels at day 3 storage at room temperature and chiller temperature were 102, 44% and 98.81%. Storage of paracetamol syrup at the chiller temperature produces smaller levels than at room temperature. Storage for three days shows a significant decrease in levels both at room temperature or chiller temperature (pvalue <0.05).

Accelerated Aging of Deacidified and Untreated Book Paper in 1967 Compared with 52 Years of Natural Aging

2020 ◽  
Vol 41 (3) ◽  
pp. 131-152
Author(s):  
Tali H. Horst ◽  
Richard D. Smith ◽  
Antje Potthast ◽  
Martin A. Hubbe
Keyword(s):  
Room Temperature ◽  
Accelerated Aging ◽  
Natural Aging ◽  
Scanning Electron Micrographs ◽  
Surface Ph ◽  
Room Temperature Storage ◽  
Folding Endurance ◽  
Endurance Tests ◽  
Breaking Length ◽  
Temperature Storage

AbstractThree copies of a book that had been optionally deacidified using two different procedures in 1967, and then subjected to accelerated aging, were tested again after 52 years of natural aging. Matched copies of the book Cooking the Greek Way, which had been printed in Czechoslovakia on acidic paper, were evaluated. Nonaqueous treatment of two of the copies with magnesium methoxide dissolved in chlorofluorocarbon solvent had been found in 1967 to have decreased the susceptibility to embrittlement, as evidenced by the results of the accelerated aging, followed by folding endurance tests. Retesting of the same books in 2019, after 52 years of room temperature storage, showed that the deacidification treatments had achieved the following benefits in comparison to the untreated book: (a) higher brightness; (b) higher folding endurance; (c) tensile breaking length higher in the cross-direction of the paper; (d) substantial alkaline reserve content, (e) an alkaline surface pH in the range 7.1–7.4, and (f) higher molecular mass of the cellulose. Remarkably, some of the folding endurance results matched those of unaged samples evaluated in 1967. Scanning electron micrographs showed no differences between the treated and untreated books.

The Effect Of Storage On Platelet Morpholog

1981 ◽  
Author(s):  
A Sturk ◽  
L M Burt ◽  
T Hakvoort ◽  
J W ten cate ◽  
N Crawford
Keyword(s):  
Electron Microscopy ◽  
Membrane Structure ◽  
Room Temperature ◽  
Surface Membrane ◽  
Platelet Concentrates ◽  
Room Temperature Storage ◽  
Short Survival ◽  
Transmission Electron ◽  
Temperature Storage ◽  
Morphological Correlation

Platelet concentrates were stored for one, two or three days at 4°C (unagitated) or room temperature (unagitated and linearly agitated). The morphology of platelets in platelet concentrates, directly after twice washing at room temperature and after 60 min incubation of the washed platelets at 37°C was investigated by both scanning and transmission electron microscopy.Platelets in the freshly prepared concentrates are slightly activated, i.e. show some pseudopod formation. At 4°C platelets rapidly loose their discoid shape. After three days their surface membrane shows extensive folding, they are slightly vacuolated and have lost most of their granules. Incubation of these cold-stored platelets at 37°C does not induce reversal to the discoid shape.Room temperature storage results in reversal of the slight initial platelet activation. After three days unagitated platelets are slightly more vacuolated than platelets stored with agitation. Room temperature storage usually results in remarkably well preserved, discoid platelets. Occasionally however, agitated platelet concentrates contain a high proportion of odd shaped cells. As platelets stored at 4°C did not became discoid after incubation at 37°, the altered membrane structure could provide an explanation for their short survival upon transfusion. Our results also provide a morphological correlation with the slightly better recovery and survival of platelets stored agitated vs.- non-agitated platelets at room temperature.

The effects of nanoscale geometry and spillover on room temperature storage of hydrogen on silica nanosprings

2013 ◽  
Vol 46 (50) ◽  
pp. 505307 ◽  
Author(s):  
Giancarlo Corti ◽  
Yingqian Zhan ◽  
Lidong Wang ◽  
Brian Hare ◽  
Timothy Cantrell ◽  
...  
Keyword(s):  
Room Temperature ◽  
Room Temperature Storage ◽  
Silica Nanosprings ◽  
Temperature Storage

Mineral Oil-Chitosan Emulsion Coatings Affect Quality and Shelf-Life of Coated Eggs during Refrigerated and Room Temperature Storage

2011 ◽  
Vol 76 (4) ◽  
pp. S262-S268 ◽  
Author(s):  
Damir D. Torrico ◽  
Hong Kyoon No ◽  
Witoon Prinyawiwatkul ◽  
Marlene Janes ◽  
José A.H. Corredor ◽  
...  
Keyword(s):  
Shelf Life ◽  
Room Temperature ◽  
Mineral Oil ◽  
Room Temperature Storage ◽  
Quality And Shelf Life ◽  
Temperature Storage

scholarly journals Evaluasi Penyimpanan Spermatozoa Ayam Pada Suhu 5⁰C, 26⁰C Dengan Pengencer Infuse NaCl, Glukosa 5% dan 10%

2021 ◽  
pp. 10-19
Author(s):  
Asnawi Asnawi ◽  
Maskur Maskur ◽  
Adji Santoso Dradjat
Keyword(s):  
Cold Storage ◽  
Room Temperature ◽  
Storage Temperature ◽  
Room Temperature Storage ◽  
C Storage ◽  
Better Than ◽  
Temperature Storage

The purpose of this study were to compare the quality of spermatozoa stored at 26⁰C, 5⁰C using diluents of NaCl, 10% glucose and 5% glucose. The spermatozoa of a rooster was collected and divided into 6 parts, each 2 tubes diluted in a ratio of 1:1 using NaCl, Glucose5% and Glucose 10%, then each 3 tubes with different diluents were stored at 26⁰C and 5⁰C. Observations of motility, viability and abnormalities of spermatozoa were carried out half an hour, 1 hour after dilution, followed every 2 hours until the ninth hours. The results showed that spermatozoa stored for 9 hours at a temperature of 26⁰C with a physiological diluent of NaCl, 10% Glucose and 5% Glucose each were different (P, < 0.05) with motility 50 ± 0.0%, 42 ± 10.95. % and 34±8.94%, respectively. At storage temperature of 5⁰C for 9 hours, physiological NaCl, 10% glucose and 5% glucose were significantly different (P<0.05) with motility 58.00±10.95%, 46.00±8.94% and 38.00±, respectively. 10.95% in a row. The viability of spermatozoa at 26⁰C storage with 5% glucose diluent was better than 10% glucose and physiological NaCl (P<0.05), 58.93±1.27%, 42.93±1.48% and 33.43±1.27% , while the physiological NaCl diluent and 10% glucose were not significantly different (P>0.05). At 5⁰C storage the viability of spermatozoa in the three diluents was not significantly different, with values of Glucose 10%, Glucose 5% and physiological NaCl 52.57±5.15%, 52.21±5.02% and 48.14±8.09%, respectively. Spermatozoa abnormalities at storage temperature 26⁰C and 5⁰C for 9 hours using physiological NaCl diluent, 5% glucose and 10% glucose, were not significantly different and varied between 5 to 10%. Finally, it can be concluded that at room temperature storage less than 4 hours the quality of spermatozoa was better with 5% glucose diluent, while for cold storage beyond 4 hours the quality of spermatozoa with NaCl diluent was higher

Effect of thermal packaging temperature on Chinese steamed bread quality during room temperature storage

2020 ◽  
Vol 92 ◽  
pp. 102921
Author(s):  
Shuya Xu ◽  
Rui Dong ◽  
Yu Liu ◽  
Xiaolong Wang ◽  
Tian Ren ◽  
...  
Keyword(s):  
Room Temperature ◽  
Bread Quality ◽  
Room Temperature Storage ◽  
Chinese Steamed Bread ◽  
Steamed Bread ◽  
Temperature Storage
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