Changes in sodium and uric acid concentrations in plasma during the menstrual cycle.

1984 ◽  
Vol 30 (3) ◽  
pp. 380-381 ◽  
Author(s):  
M Mira ◽  
P M Stewart ◽  
V Gebski ◽  
D Llewellyn-Jones ◽  
S F Abraham
Keyword(s):  
Uric Acid ◽  
Menstrual Cycle ◽  
Luteal Phase ◽  
Reference Interval ◽  
Hormonal Changes ◽  
Biochemical Variables ◽  
Normal Reference

Abstract Hormonal changes during the menstrual cycle are well documented, but many other biochemical variables have not been studied. We find that in the luteal phase of the menstrual cycle the concentrations of sodium and uric acid are significantly lower. The changes may be of significance for the determination of the normal reference interval.

scholarly journals The levels of adipokines in relation to hormonal changes during the menstrual cycle in young, normal-weight women

2017 ◽  
Vol 6 (8) ◽  
pp. 892-900 ◽  
Author(s):  
Katarzyna Wyskida ◽  
Grzegorz Franik ◽  
Tomasz Wikarek ◽  
Aleksander Owczarek ◽  
Alham Delroba ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Multivariate Regression ◽  
Normal Weight ◽  
Total Testosterone ◽  
Hormonal Changes ◽  
Regression Analyses ◽  
Anthropometric Parameters ◽  
Tnf Α ◽  
Plasma Leptin

Context The aim of this study was to assess the plasma leptin, adiponectin, resistin, visfatin/NAMPT, omentin-1, vaspin, apelin, TNF-α, IL-6 and RBP4 levels in relation to hormonal changes during the menstrual cycle in young, healthy, normal-weight women. Methods The study involved 52 young, healthy, normal-weight women. Anthropometric parameters, body composition and levels of plasma leptin, adiponectin, resistin, visfatin/NAMPT, omentin-1, vaspin, apelin, TNF-α, IL-6 and RBP4 in addition to serum FSH, LH, estradiol, progesterone, 17-OH progesterone, androgens, SHBG and insulin concentrations were measured during a morning in fasting state three times: between days 2–4, days 12–14 and days 24–26 of the menstrual cycle. Results Plasma adiponectin, omentin-1, resistin and visfatin/NAMPT, apelin, TNF-α, IL-6 and RBP4 concentrations were stable during the menstrual cycle, while leptin and vaspin levels were significantly higher in both the midcycle and the luteal phases than those in the follicular phase. Multivariate regression analyses revealed that changes in leptin and vaspin levels between the follicular and the luteal phase are strongly related to changes in total testosterone levels. Conclusions Our results revealed stable levels of adipokines during the phases of the physiological menstrual cycle, except for leptin and vaspin, which showed increased levels in both the midcycle and the luteal phases. This effect was significantly associated with changes in the secretion of testosterone, 17-OH progesterone and insulin in the luteal phase.

Hubungan Antara Status Gizi Dan Tingkat Stres Dengan Kejadian Pre-Menstrual Syndrome Pada Remaja Putri Di Pondok Pesantren Lirboyo Kota Kediri Tahun 2016

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Miftakhul Mualimah ◽  
Ana Wildani
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Simple Random Sampling ◽  
Physical Symptoms ◽  
Nutrition Status ◽  
P Value ◽  
Hormonal Changes ◽  
Cross Sectional ◽  
Stress Levels ◽  
Current Cycle

ABSTRAK Pre-Menstrual Syndrome adalah kumpulan gejala fisik, psikologis dan emosi yang terkait dengan siklus menstruasi wanita dan secara konsisten terjadi selama fase luteal dari siklus menstruasi akibat perubahan hormonal yang berhubungan dengan siklus saat ovulasi dan menstruasi. Salah satu faktor penyebab pre-menstrual syndrome adalah status gizi dan tingkat stres.Desain penelitian yang digunakan adalah penelitian analitik korelasional dengan menggunakan pendekatan cross sectional. Populasi yang diteliti adalah semua remaja putri usia 12-21 tahun di Pondok Pesantren Lirboyo pada Tahun 2016 sejumlah 127 orang dengan teknik simple random sampling analisa dengan menggunakan spearman rank.Hasil analisis diperoleh nilai p value = 0,000 sehingga  p value  α  maka H0 ditolak dan H1 diterima dengan nilai r =- 0,533. Sedangkan variabel tingkat stres dengan kejadian pre-menstrual syndrome, dengan hasil p value = 0,000 sehingga  p value  α  maka H0 ditolak dan H1 diterima dengan nilai r = 0,650. Berdasarkan hasil penelitian diharapkan angka kejadian pre-menstrual syndrome dapat berkurang dengan cara mengenali dini gejala terjadinya pre-menstrual syndrome dan memberikan penyuluhan kepada santri cara mencegah pre-menstrual syndrome. Kata kunci : status gizi, tingkat stres dan pre-menstrual syndrome.   ABSTRACT Pre-Menstrual Syndrome is collection of physical symptoms, psychological and emotion associated with a woman’s menstrual cycle and consistently occur during the luteal phase of the menstrual cycle due to hormonal changes associated with the current cycle of ovulation and menstruation. One of couses factor pre-menstrual syndromeis nutrition status and stress levels. The research design used an analytic corelation research by using the cross sectional approach. The population of this research were young women aged 12-21 years at Pondok Pesantren Lirboyo in 2016 amount 127 peoples with probability sampling type analyse by spearman rank.The analysis result showed that between nutrition status with pre-menstrual syndrome that p value = 0.000, p value α so H0 was rejected and H1 was accepted by r =-0,533.Futhermore, the result showed that between stress levels with pre-menstrual syndrome, get p value = 0.000 so p value α, H0was rejected and H1 was acceptedby r = 0,650. Based on this result was expected that the incident of pre-menstrual syndromecan be decrease by recognize the symptomps of pre-menstrual syndrome and provide counseling to students how to prevent pre-menstrual syndrome. Keywords : nutrition status, stress levels and pre-menstrual syndrome

Kinetic determination of serum adenosine deaminase

1993 ◽  
Vol 39 (10) ◽  
pp. 2182-2185 ◽  
Author(s):  
H M Oosthuizen ◽  
J P Ungerer ◽  
S H Bissbort
Keyword(s):  
Uric Acid ◽  
Adenosine Deaminase ◽  
Calibration Curve ◽  
Kinetic Method ◽  
Reference Interval ◽  
Enzymatic Method ◽  
Established Method ◽  
Kinetic Determination ◽  
Automated Assay

Abstract A new kinetic method for the determination of serum adenosine deaminase (EC 3.5.4.4) is described, with adenosine as the substrate and nucleoside phosphorylase and xanthine oxidase as the reaction enzymes. Inosine is produced, which is converted to hypoxanthine. The hypoxanthine is oxidized to xanthine, which is further oxidized to uric acid. In these two reactions, blue 2,6-dichlorophenolindophenol is reduced to a colorless compound and the decrease in color is measured spectrophotometrically at 606 nm. The assay was automated by using a Cobas Mira analyzer. The automated assay had a CV of < 7%, and the calibration curve was linear from 10 to 120 U/L. The assay correlates well with an established method, based on detection of liberated NH3 with Berthelot's reaction. The reference interval (mean +/- 2 SD) was 14-34 U/L (mean 24 U/L, n = 84). The enzymatic method described is easily automated and seems to be suitable for the routine determination of adenosine deaminase in serum.

Indices of ovulation: comparison of plasma and salivary levels of progesterone with urinary pregnanediol

1984 ◽  
Vol 100 (1) ◽  
pp. 75-80 ◽  
Author(s):  
M. G. Metcalf ◽  
J. J. Evans ◽  
J. A. Mackenzie
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Follicular Phase ◽  
The Other ◽  
Urine Samples ◽  
Median Increase ◽  
Daily Excretion ◽  
Alternative Procedures ◽  
Biochemical Assessment

ABSTRACT An increased daily excretion of pregnanediol, relative to that early in the menstrual cycle, is often taken to be evidence that a woman has ovulated. This paper assesses the value of alternative procedures for this purpose. Urine, plasma and saliva samples were collected during a 24-h period from 20 women during the follicular phase and from 20 women during the luteal phase. The 24-h excretion of pregnanediol was compared with (1) the concentration of progesterone in plasma, (2) the concentration of progesterone in saliva, (3) the concentration of pregnanediol in small urine samples, (4) the rate of excretion of pregnanediol and (5) the ratio of pregnanediol to creatinine in small urine samples. Each analyte increased substantially during the luteal phase. The median increases (ratio of luteal to follicular phase values) were 14·8, 3·2, 10·6, 11·9 and 11·1 respectively. By comparison, the median increase in 24-h pregnanediol output was 9·2. When the other analytes were used instead of the 24-h excretion of pregnanediol to assess the possibility of ovulation, the incidence of misclassifications (follicular samples classed as luteal and luteal samples classed as follicular) was 0, 12·8, 5·9, 2·0 and 1·0% respectively. It was concluded that the most satisfactory alternative to the measurement of 24-h pregnanediol output for the biochemical assessment of ovulation based on progesterone production was the measurement of the concentration of progesterone in plasma; the least satisfactory alternative was determination of the concentration of progesterone in saliva. If blood was not available, measurement of the ratio of pregnanediol to creatinine in a small urine sample was the preferred method. J. Endocr. (1984) 100, 75–80

scholarly journals Paranoid Hostility but Not Depressive Symptoms Associated with Cytokines During the Premenstrual Phase in Young Women

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Syros Ioannis
Keyword(s):  
Depressive Symptoms ◽  
Menstrual Cycle ◽  
Young Women ◽  
Proinflammatory Cytokines ◽  
Luteal Phase ◽  
Depression Scale ◽  
Rank Correlation ◽  
Hormonal Changes ◽  
Mean Values ◽  
Tnf Α

It is well known that some women, during their premenstrual phase, express Hostility (Ho) and Depressive symptoms (De). Various studies indicate that Ho and De correlate with circulating concentrations of cytokines. These data support the hypothesis of an inflammation-sensitive mechanism linked to certain psychological states. The aim of this study was to correlate Ho and De with selective proinflammatory cytokines during the menstrual cycle in young women. Twenty-two physically healthy medical students, mean age 22.95+2.83(SD), were studied. All reported regular menses that ranged between 27-33 days. Blood was drawn three times across a single cycle: at follicular phase (FL), at mid luteal phase (ML) and at late luteal phase (LL) corresponding to the premenstrual phase. The following proinflammatory cytokines were determined at each sample: Interleukin 8 (IL-8), Interleukin 6 (IL-6), Interleukin 1α (IL-1α), and Tumor Necrosis Factor-α (TNF-α). Immediately before blood sampling the women completed the Zung self-rating depression scale and the hostility and direction of hostility questionnaire (HDHQ), which comprises five subscales: urge to act out hostility, criticism of others, paranoid hostility, self-criticism and delusional guilt. Spearman’s rank correlation was used to determine the relation between variables at the specific time points; ρ<0.05 was considered significant. Mean values of cytokines did not change significantly throughout the menstrual cycle. Paranoid hostility was positively correlated with IL-8 (Rs=0.476, p=0.046), IL-1α (Rs=0.458, p=0.048) and TNF-α (Rs=0.518, p=0.023) in LLphase. No other significant correlation was found. Cytokines correlate with paranoid hostility and this correlation is probably related with the premenstrual hormonal changes. It is difficult to evaluate the clinical significance of our findings, nevertheless, when exploring the above association, it is necessary to take into consideration the phase of the cycle as well as the special aspects of Ho we seek to study.

A MODIFIED METHOD FOR THE QUANTITATIVE DETERMINATION OF PROGESTERONE IN HUMAN PLASMA

1963 ◽  
Vol 43 (1) ◽  
pp. 101-109 ◽  
Author(s):  
Ian F. Sommerville ◽  
Mary T. Pickett ◽  
William P. Collins ◽  
Diane C. Denyer
Keyword(s):  
Menstrual Cycle ◽  
Quantitative Determination ◽  
Human Plasma ◽  
Peripheral Blood ◽  
Luteal Phase ◽  
Chemical Method ◽  
Blood Levels ◽  
Modified Method

ABSTRACT A more sensitive and less complicated chemical method has been evolved for the quantitative determination of progesterone in 5–10 ml samples of human plasma. The method is suitable for application to peripheral blood levels at all stages of pregnancy and may be applied to the study of the luteal phase of the menstrual cycle. The sensitivity, accuracy, precision and specificity of the method are discussed.

THE FLUORIMETRIC DETERMINATION OF PROGESTERONE IN HUMAN PLASMA DURING PREGNANCY AND THE MENSTRUAL CYCLE

1962 ◽  
Vol 25 (2) ◽  
pp. 239-244 ◽  
Author(s):  
R. V. SHORT ◽  
IRIS LEVETT
Keyword(s):  
Menstrual Cycle ◽  
Pregnant Women ◽  
Human Plasma ◽  
Peripheral Blood ◽  
Luteal Phase ◽  
Follicular Phase ◽  
Fluorimetric Determination ◽  
Fluorescence Reaction ◽  
Good Agreement

SUMMARY The fluorescence reaction for progesterone described by Touchstone & Murawec (1960) has been used to determine the concentration of progesterone in nineteen samples of peripheral blood from pregnant women, and in seventeen samples of peripheral blood from women during the course of the menstrual cycle. There was good agreement between the ultraviolet and fluorescent estimates of progesterone in all the samples from pregnant women. The concentrations found during the follicular phase of the menstrual cycle were in general lower than those found during the luteal phase. In one woman who was sampled repeatedly during the course of a menstrual cycle, there was a well defined rise in the level of progesterone in the blood after the expected date of ovulation.

Sign in / Sign up

Export Citation Format

Share Document