Zinc content of cellular components of blood: methods for cell separation and analysis evaluated.

1985 ◽  
Vol 31 (1) ◽  
pp. 65-69 ◽  
Author(s):  
D B Milne ◽  
N V Ralston ◽  
J C Wallwork
Keyword(s):  
Atomic Absorption ◽  
Zinc Content ◽  
Atomic Absorption Spectrophotometry ◽  
Gradient System ◽  
Good Separation ◽  
Modified Technique ◽  
Matrix Interferences ◽  
Flame Atomic Absorption ◽  
Degree Of Separation ◽  
Cellular Components

Abstract Platelets, mononucleated cells, polymorphonucleated cells, and erythrocytes were separated from whole blood by use of discontinuous gradients of colloidal polyvinylpyrrolidone-coated silica ("Percoll"). We measured the zinc content of these cells by flame atomic absorption spectrophotometry, using a modified technique for micro-samples that obviated matrix interferences. Thus, results obtained by conventional flame atomic absorption and by the micro-method were identical. Inter-comparisons of separation methods indicated that separation of platelets and mononucleated cells by a two-gradient system of "Ficoll-Hypaque" (a synthetic polymer of sucrose) or Percoll was relatively poor, whereas there was a good separation when a tertiary gradient system of Percoll was used. The apparent zinc content of mononucleated cells depended on the degree of separation from the platelets, with contamination by platelets resulting in artificially high values for mononucleated cells.

Use of a flow-injection sample manipulator as an interface between a "high-performance" liquid chromatograph and an atomic absorption spectrophotometer.

1981 ◽  
Vol 27 (9) ◽  
pp. 1546-1550 ◽  
Author(s):  
B W Renoe ◽  
C E Shideler ◽  
J Savory
Keyword(s):  
Atomic Absorption ◽  
Flow Injection ◽  
Metal Binding ◽  
High Performance ◽  
Atomic Absorption Spectrophotometry ◽  
Clinical Samples ◽  
High Performance Liquid Chromatograph ◽  
Liquid Chromatograph ◽  
Flame Atomic Absorption ◽  
Column Eluate

Abstract We describe an integrated, molecular-absorbance, atomic absorption instrument for studying metal/ligand binding in clinical samples. For an interface between the "high-performance" liquid chromatograph and the atomic absorption instrument we used a flow-injection sample manipulator, thus allowing both the chromatograph and the atomic absorption detector to operate at their separate optimum conditions. After specimen separation with a gel permeation column, we measured the molecular components of the column eluate by molecular absorbance spectrometry and the atomic components (calcium and magnesium) by flame atomic absorption spectrophotometry. This instrument system is capable of separating and analyzing multiple components within 20 min of injection of the sample on the column. The chromatograms presented demonstrate the utility of the system for investigating metal binding to a variety of ligands in clinical samples.

Reliable determination of copper complex ions in synthetic wastewater using flame atomic absorption spectrophotometry

2019 ◽  
Vol 79 (5) ◽  
pp. 833-841 ◽  
Author(s):  
Kanokporn Supong ◽  
Parnuwat Usapein
Keyword(s):  
Atomic Absorption ◽  
Copper Complexes ◽  
Atomic Absorption Spectrophotometry ◽  
Synthetic Wastewater ◽  
Flame Atomic Absorption Spectrophotometry ◽  
Working Standard ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Relative Standard ◽  
Delay Times

Abstract Forms of copper that are highly soluble in aquatic environments are used as chemical reagents in a variety of industries, especially copper complexes. Wastewater containing copper complexes can be difficult to treat and analyse. Normally, flame atomic absorption spectrophotometry (FAAS) is a favourable technique for analysing metal ions in wastewater, but is less reliable for copper analysis owing to the influence of copper oxides. To solve this problem, it is necessary to adjust the atomization and delay times. The objective of this study was to use FAAS to accurately determine the quantity of complexed copper in synthetic wastewater, using increased atomization and delay times. The method showed excellent linearity in the copper standard concentration range of 1–5 mg L−1. The sensitivity of the analysis was 0.023 mg L−1. The percentage of recovery and the relative standard deviation were 85.02% and 0.75%, respectively. The experimental results demonstrated that the estimation of uncertainty from preparation of working standard, repeatability uncertainty, instrument deviation, calibration curve and recovery uncertainty were 8.2 × 10−4, 4.49 × 10−4, 7.21 × 10−4, 21.25 × 10−4, and 98.19 × 10−4, respectively. Overall, the results showed the suitability of the FAAS method for determining copper in synthetic wastewater.

Influence of Phosphorus and Calcium on Flame Atomic Absorption Spectrophotometric Determination of Lead in Canned Fish Products

1984 ◽  
Vol 67 (1) ◽  
pp. 186-187
Author(s):  
Teresa M Serra ◽  
J F Vale Serrano
Keyword(s):  
Atomic Absorption ◽  
Atomic Absorption Spectrophotometry ◽  
Flame Atomic Absorption Spectrophotometry ◽  
Experimental Conditions ◽  
Fish Products ◽  
Real Samples ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Canned Fish

Abstract Lead was determined in the presence of whole multiples of the P/Ca ratio found in Portuguese canned fish by flame atomic absorption spectrophotometry with and without using an ashing aid. Under our experimental conditions, use of the ashing aid eliminates P and Ca interference. Results with real samples, spiked with 1, 2,3, and 4 ppm lead, are presented and statistically treated.

Optimized atomic absorption spectrophotometry of calcium in erythrocytes.

1987 ◽  
Vol 33 (11) ◽  
pp. 2004-2007 ◽  
Author(s):  
S Nomoto ◽  
S Shoji
Keyword(s):  
Hydrochloric Acid ◽  
Atomic Absorption ◽  
Atomic Absorption Spectrophotometry ◽  
Graphite Tube ◽  
Optimum Conditions ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Flame Atomic Absorption Spectrophotometer ◽  
The Mean ◽  
Mean Concentration

Abstract We sought to establish optimum conditions for measuring calcium in erythrocytes by atomic absorption spectrophotometry. The conditions we selected are as follows. Wash one volume of fresh heparin-treated packed cells once with 30 volumes of isotonic buffered saline (pH 7.4) at a temperature somewhat exceeding 25 degrees C. Dilute the washed packed cells 10-fold with 12 mmol/L hydrochloric acid, and analyze the supernate for calcium. Measure the hematocrit of the washed packed cells, then analyze an aliquot of them for calcium, using a computer-readout type of flame or a non-flame atomic absorption spectrophotometer equipped with a pyrocoated graphite tube. The temperature program is 1000 degrees C for ashing [corrected] and 1800 degrees C for the atomizing cycle. Intraday and day-to-day reproducibility of the assay was 6.55% and 8.19%, respectively, at the mean concentration of calcium in the erythrocytes of healthy adults, which is 4.30 mumol/L.

Microwave Acid Digestion of Various Food Matrixes for Nutrient Determination by Atomic Absorption Spectrophotometry

1991 ◽  
Vol 74 (5) ◽  
pp. 812-814 ◽  
Author(s):  
Philip J Oles ◽  
Wanda M Graham
Keyword(s):  
Atomic Absorption ◽  
Microwave Digestion ◽  
Manganese Content ◽  
Control Sample ◽  
Atomic Absorption Spectrophotometry ◽  
Breakfast Cereal ◽  
Hot Plate ◽  
Absorption Spectrophotometry ◽  
Flame Atomic Absorption ◽  
Microwave Apparatus

Abstract Food matrixes were digested by treatment with nitric acidsulfuric acid-hydrogen peroxide mixtures and heating with a commercially available microwave apparatus. The digests were analyzed for sodium, potassium, magnesium, iron, calcium, zinc, copper, and manganese content by flame atomic absorption spectrophotometry. A total of 3 digestion programs were adopted for a variety of food matrixes. Microwave digestion times of 30-45 mln were normally sufficient for obtaining clear digests compared to hot plate digestion times of 8-12 h. Precision for microwave digestion of most nutrients was equal to or better than that of hot plate digestion. Microwave digestion gave comparable results for the nutrient elements. National Institute of Standards and Technology reference materials were analyzed for nutrient content after microwave digestion and the results compared favorably with the certified values. A laboratory breakfast cereal control sample with a history of over 20 separate hot plate digestions was prepared using the microwave technique. Results for the 8 elements were within ±7% of the hot plate digestion values. Predlgestlon spikes were recovered from several food matrixes with no effects resulting from Incomplete digestion of samples.

Co-precipitation of Ni, Cr, Mn, Pb and Zn in industrial wastewater and sediment samples with copper(II) cyclo-hexylmethyldithiocarbamate for their flame atomic absorption spectrometric determination

2012 ◽  
Vol 66 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Ayodele Rotimi Ipeaiyeda ◽  
Adekunle Johnson Odola
Keyword(s):  
Heavy Metals ◽  
Atomic Absorption ◽  
Industrial Wastewater ◽  
Atomic Absorption Spectrophotometry ◽  
Sample Volume ◽  
Sediment Samples ◽  
Flame Atomic Absorption ◽  
Precipitation Procedure ◽  
Co Precipitation

A co-precipitation technique for nickel(II), chromium(II), manganese(II), lead(II) and zinc(II) with the aid of copper(II) cyclo-hexylmethyldithiocarbamate was established. The influences of some analytical parameters such as pH, sample volume, amounts of cyclo-hexylmethyldithiocarbamate and copper(II) on the recovery of metal ions were investigated. The heavy metals in the precipitate were determined by flame atomic absorption spectrophotometry. The range of detection limits for the heavy metals was 0.003–0.005 mg/L. The atomic spectrometric technique with co-precipitation procedure was successfully applied for the determination of Ni, Cr, Mn, Pb and Zn in industrial wastewater and sediment samples from Ladipo stream in Lagos, Nigeria. The mean concentrations for these metals using co-precipitation procedure were not significantly different from corresponding concentrations obtained using spectrometric techniques without co-precipitation procedure.

Sign in / Sign up

Export Citation Format

Share Document