Concentrations of iodothyronines in serum of patients with chronic renal failure and other nonthyroidal illnesses: role of free fatty acids.

Abstract The mean concentration of free thyroxin (FT4) in serum, as determined by direct equilibrium dialysis, was decreased in patients with chronic renal failure (CRF) and increased in patients with various other nonthyroidal illnesses (NTI). The mean concentration of dialyzable free triiodothyronine (FT3) in serum was equally low in both groups of patients. Patients with CRF of various etiology but a similar degree of renal failure as estimated from serum creatinine assay had very similar concentrations of FT4 and FT3 in their serum. Mean thyroxin (T4) and triiodothyronine (T3) concentrations in serum were decreased in CRF and NTI, whereas the mean reverse-T3 concentration in serum was normal in CRF and increased in NTI. T4-binding globulin and albumin were markedly decreased in CRF and NTI; T4-binding prealbumin was increased in CRF and decreased in NTI. The mean concentration of nonesterified free fatty acids (FFA) in serum was increased in NTI but not in CRF. The weak, but significant, positive correlation observed between FT4 and FFA in serum (r = 0.34, P less than 0.01) in NTI indicates that the increase in serum FT4 in this group of patients could be an effect, at least in part, of FFA competing with T4 for binding sites on serum proteins. The stronger correlation detected between the serum FT4 concentration and the FFA/albumin molar ratio in serum (r = 0.60, P less than 0.001) demonstrates the importance of a low albumin concentration for expression of the effect of FFA on FT4 in severe systemic illnesses.

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A radioimmunoassay for 1,25-dihydroxycholecalciferol.

Clinical Chemistry ◽

10.1093/clinchem/26.5.562 ◽

1980 ◽

Vol 26 (5) ◽

pp. 562-567 ◽

Cited By ~ 133

Author(s):

R Bouillon ◽

P De Moor ◽

E G Baggiolini ◽

M R Uskokovic

Keyword(s):

Renal Failure ◽

Chronic Renal Failure ◽

High Titer ◽

Rabbit Serum ◽

Normal Rabbit Serum ◽

Large Excess ◽

High Affinity ◽

The Mean ◽

Mean Concentration ◽

25 Hydroxycholecalciferol

Abstract We describe a radioimmunoassay for 1,25-dihydroxycholecalciferol in human serum. We raised antisera in rabbits to 1,25-dihydroxycholecalciferol-3-hemisuccinate coupled to bovine serum albumin, and obtained sensitive, high-titer antibodies. These antibodies had a high affinity for 1,25-dihydroxycholecalciferol and cross reacted mainly with 25-hydroxycholecalciferol and 24,25-dihydroxycholecalciferol. Addition of 1 mL of normal rabbit serum per liter reduced this interference to 5 and 4%, respectively. However, these interfering steroids are present in large excess, so extensive purification of 1,25-dihydroxycholecalciferol from serum is necessary. The steroid was extracted with ethyl acetate/cyclohexane, purified on Sephadex LH-20, and then chromatographed on a column of silicic acid. The radioimmunoassay is sensitive to 5 pg/tube (3 ng/L of serum). The between-assay CV was 14%. The mean concentration of 1,25-dihydroxycholecalciferol in the serum of 54 healthy adults was 38 (SD 12) ng/L, with no sex-related difference. The assay was further validated by the finding of low or undetectable concentrations in patients with chronic renal failure and of increased concentrations in the serum of patients with primary hyperparathyroidism. In comparison with previously described methods, the major advantage of the present assay is the use of stable gamma-globulins, which are available in large amounts, as binding protein.

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A radioimmunoassay for 1,25-dihydroxycholecalciferol.

Clinical Chemistry ◽

10.1093/clinchem/26.5.0562 ◽

1980 ◽

Vol 26 (5) ◽

pp. 562-567 ◽

Cited By ~ 3

Author(s):

R Bouillon ◽

P De Moor ◽

E G Baggiolini ◽

M R Uskokovic

Keyword(s):

Renal Failure ◽

Chronic Renal Failure ◽

High Titer ◽

Rabbit Serum ◽

Normal Rabbit Serum ◽

Large Excess ◽

High Affinity ◽

The Mean ◽

Mean Concentration ◽

25 Hydroxycholecalciferol

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Evaluation of serum free fatty acids in chronic renal failure: evidence from a rare case with undetectable serum free fatty acids and population data

Lipids in Health and Disease ◽

10.1186/s12944-019-1093-5 ◽

2019 ◽

Vol 18 (1) ◽

Cited By ~ 1

Author(s):

Zhen-Xian Liu ◽

Qian Hong ◽

Ding-Hui Peng ◽

Ying Yang ◽

Wen-Li Yu ◽

...

Keyword(s):

Fatty Acids ◽

Renal Failure ◽

Chronic Renal Failure ◽

Free Fatty Acids ◽

Rare Case ◽

Population Data ◽

Serum Free Fatty Acids ◽

Serum Free ◽

Undetectable Serum

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Lack of relationship between serum free fatty acids and impaired plasma protein binding of diphenylhydantoin in chronic renal failure

European Journal of Clinical Pharmacology ◽

10.1007/bf00563076 ◽

1976 ◽

Vol 10 (6) ◽

pp. 403-405 ◽

Cited By ~ 10

Author(s):

I. Odar-Cederl�f ◽

O. Borg�

Keyword(s):

Fatty Acids ◽

Renal Failure ◽

Chronic Renal Failure ◽

Protein Binding ◽

Free Fatty Acids ◽

Plasma Protein Binding ◽

Plasma Protein ◽

Serum Free Fatty Acids ◽

Serum Free

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RELATIONSHIP BETWEEN CARDIOVASCULAR DISEASES AND ANATOMOSIS OF ARTERIOVENOUS FISTULAR IN PATIENTS WITH REGULARLY HEMODIALYSIS

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2017.3.13 ◽

2017 ◽

pp. 88-92

Author(s):

Van Hien Pham ◽

Huu Vu Quang Nguyen ◽

Tam Vo

Keyword(s):

Heart Failure ◽

Blood Pressure ◽

Renal Failure ◽

Chronic Renal Failure ◽

Cardiovascular Diseases ◽

Systolic Blood Pressure ◽

Diastolic Blood Pressure ◽

Negative Correlation ◽

Mean Value ◽

The Mean

Background: Cardiovascular diseases are the leading cause of death in patients with chronic renal failure. When a patient undergoes dialysis, making AVF or AVG causes cardiovascular events. Understanding the relationship between complications: hypertension, heart failure, AVF or AVG (formation time, position, diameter) helps us monitor, detect, prevent and treatment of complications to limit the risk of death in patients with dialysis. Objective: Relationship between cardiovascular diseases and anatomosis of arteriovenous fistular in patients with regularly hemodialysis at Cho Ray Hospital. Methods: A cross-sectional study was conducted at Cho Ray Hospital from 2015 to 2016. The survey some cardiovascular diseases are done by clinical examination, tests for diagnostic imaging such as X-ray, electrocardiogram and echocardiogram: heart and diameter of anastomosis AVF, AVG. Results: The study population included 303 patients with chronic renal failure who were dialysis. Of which, patients aged 25-45 accounted for the highest proportion (43.9%). The proportion of male and female patients was similar (48.5% and 51.5% respectively). The mean value of systolic blood pressure on patients made AVF, AVG less than 12 months is higher than patients made AVF, AVG over 12 months, and there is negative correlation (r = -0.43) between AVF, AVG and systolic blood pressure (p <0.05). The mean value of diastolic blood pressure on patients made AVF, AVG less than 12 months is lower than patients made AVF, AVG over 12 months, and and there is positive correlation (r = -0.43) between AVF, AVG and diastolic blood pressure (p <0.05) (p <0.05). The prevalence of patients with heart failure made AVF, AVG over 12 months is higher than that of the under 12 months group, there is a negative correlation (r = - 0.43) between AVF, AVG diameter and EF index (p <0.05). Conclusion: It is important to note the diameter of anastomosis AVF, AVG in patients with chronic renal failure dialysis to limit cardiovascular complications, especially heart failure. Key words: Chronic kidney disease, hemodialysis.

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Oral and Maxillofacial Manifestations of Mineral and Bone Disorders Associated with Chronic Renal Failure

Revista de Chimie ◽

10.37358/rc.17.6.5667 ◽

2017 ◽

Vol 68 (6) ◽

pp. 1325-1328

Author(s):

Andrada Raluca Doscas ◽

Mihail Balan ◽

Mihai Liviu Ciofu ◽

Doriana Agop Forna ◽

Marius Cristian Martu ◽

...

Keyword(s):

Chronic Kidney Disease ◽

Renal Failure ◽

Renal Function ◽

Chronic Renal Failure ◽

Health Concern ◽

Oral Manifestations ◽

End Stage Renal Failure ◽

Brown Tumors ◽

The Mean ◽

End Stage

Chronic kidney disease (CKD) is a multifactorial syndrome and a global health concern. As renal function declines, there is a progressive deterioration of mineral homeostasis. Starting from stage 3 of CKD oral manifestations of mineral disorders can occasionally appear and become more frequent and evident in stage 5. We retrospectively analysed 43 patients diagnosed with end stage renal failure undergoing dialysis, hospitalized in our clinic for different oral and maxillofacial pathologies. The mean dialysis period was 5.43 years. Radiographic alterations afecting the jaws were found in all patients. The most common feature was partial or total loss of lamina dura, followed by alterations of the bony trabeculae. 9 patients presented brown tumors which are considered the final stage of secondary hyperparathyroidism associated with renal failure.

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Effect of Hemodialysis on the Content of Fatty Acids in Monolayers of Erythrocyte Membranes in Patients with Chronic Renal Failure

Renal Failure ◽

10.1080/08860220701260602 ◽

2007 ◽

Vol 29 (4) ◽

pp. 447-452 ◽

Cited By ~ 7

Author(s):

Barbara Dolegowska ◽

Ewa Kwiatkowska ◽

Teresa Wesolowska ◽

Joanna Bober ◽

Dariusz Chlubek ◽

...

Keyword(s):

Fatty Acids ◽

Renal Failure ◽

Chronic Renal Failure ◽

Erythrocyte Membranes

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Branched-chain amino acid supplementation augments plasma ammonia responses during exercise in humans

Journal of Applied Physiology ◽

10.1152/jappl.1993.74.6.2711 ◽

1993 ◽

Vol 74 (6) ◽

pp. 2711-2717 ◽

Cited By ~ 33

Author(s):

D. A. MacLean ◽

T. E. Graham

Keyword(s):

Fatty Acids ◽

Amino Acid ◽

Free Fatty Acids ◽

Amino Acid Supplementation ◽

Placebo Administration ◽

Branched Chain Amino Acid ◽

Placebo Trial ◽

The Mean ◽

Branched Chain ◽

Exercise In Humans

This study examined the effects of branched-chain amino acid (BCAA) supplementation on amino acid and ammonia (NH3) responses during prolonged exercise in humans. Seven men cycled for 60 min at 75% of maximal O2 uptake after 45 min of either placebo (dextrose, 77 mg/kg) or BCAA (leucine + isoleucine + valine, 77 mg/kg) supplementation. Plasma samples (antecubital vein) were collected at rest and during exercise and analyzed for plasma NH3 and amino acids, whole blood glucose and lactate, and serum free fatty acids and glycerol. After BCAA administration, plasma BCAA levels increased from 375 +/- 22 to 760 +/- 80 microM (P < 0.05) by the onset of exercise and remained elevated throughout the experiment. Plasma NH3 concentrations increased continually during exercise for both trials and were higher (P < 0.05) after BCAA supplementation than after placebo administration. The mean plasma NH3 increase from rest to 60 min was 79 +/- 10 and 53 +/- 4 microM for BCAA and placebo trials, respectively. Plasma alanine and glutamine concentrations were elevated (P < 0.05) during exercise for both treatments. However, only glutamine concentrations were greater (P < 0.05) for BCAA trial than for placebo trial during exercise. There were no significant differences between treatments for glucose, lactate, free fatty acids, and glycerol or any other plasma amino acid. These data suggest that increased BCAA availability before exercise, when initial muscle glycogen is normal, results in significantly greater plasma NH3 responses during exercise than does placebo administration.

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Stable Strontium Absorption as a Measure of Intestinal Calcium Absorption: Comparison with the Double-Radiotracer Calcium Absorption Test

Clinical Science ◽

10.1042/cs0870363 ◽

1994 ◽

Vol 87 (3) ◽

pp. 363-368 ◽

Cited By ~ 21

Author(s):

Aubrey Blumsohn ◽

Brian Morris ◽

Richard Eastell

Keyword(s):

Renal Failure ◽

Chronic Renal Failure ◽

Time Course ◽

Calcium Absorption ◽

Quadratic Term ◽

The Mean ◽

Stable Strontium ◽

The Relationship ◽

Dual Tracer ◽

Fractional Absorption

1. Stable strontium (Sr) has been proposed as an alternative to calcium (Ca) isotopes for the measurement of intestinal Ca absorption. The aim of this study was to compare the time course and fractional absorption of Ca and Sr, when both are measured using dual-tracer techniques. 2. 45Ca and Sr absorption tests were carried out on consecutive days in patients with osteoporosis (n = 10) or chronic renal failure (n = 7). Both tests were repeated in four patients with chronic renal failure after treatment with calcitriol (1 μg daily for 10 days). 3. The time course of Ca absorption was determined using the 85Sr (intravenous)/45Ca (oral) dual-tracer technique, and the time course of Sr absorption using 85Sr (intravenous)/stable Sr (oral). Oral tracers were administered on consecutive days with a test meal containing 5.3 mmol of Ca and 2.5 mmol of either stable Sr or Ca carrier. The fractional absorption of 45Ca and Sr at 6 h (FA360) and the absorption rate as a function of time were calculated by deconvolution. 4. The mean FA360 for Sr (20.2%) was lower than the mean FA360 for 45Ca (37.8%, P < 0.001, paired t-test), but the time course of Sr absorption was similar to that of Ca. There was a significant correlation between the FA360 for 45Ca and Sr, although the relationship was improved by including a quadratic term (R2 = 0.89, P < 0.001, significance of quadratic term, P < 0.05). After 1,25-dihydroxyvitamin D treatment, the FA360 of stable Sr increased 4.29-fold, whereas the FA360 of 45Ca increased only 2.4-fold. 5. Although the fractional absorption of Sr determined by dual-tracer deconvolution was the best predictor of FA360 for 45Ca, little was lost by confining the analysis to a single serum Sr measurement taken 3 h or more after oral administration. 6. We conclude that Sr absorption is qualitatively similar to that of Ca, although absorption of Sr is much lower than that of Ca. Furthermore, the relationship does not appear to be linear. Stable Sr may be useful in place of Ca isotopes in the routine clinical evaluation of Ca absorption.

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