Pre-analytical variation in alanine aminotransferase.

1988 ◽  
Vol 34 (4) ◽  
pp. 744-745 ◽  
Author(s):  
S G Ruby ◽  
N E Reiber ◽  
R E Lonser
Keyword(s):  
Analysis Of Variance ◽  
Whole Blood ◽  
Alanine Aminotransferase ◽  
Room Temperature ◽  
Normal Activity ◽  
Blood Samples ◽  
Activity Concentrations ◽  
Whole Blood Samples ◽  
Blood Specimens ◽  
Analytical Variation

Abstract To determine the effect of pre-analytical variation of alanine aminotransferase in blood specimens with normal activity concentrations of this enzyme, we stored serum and whole blood samples at 4 and 22 degrees C and assayed aliquots of each specimen at intervals up to 72 h. Analysis of variance revealed no statistically significant increase or decrease in activity of the enzyme for up to 72 h in either specimen type, whether stored at room temperature or refrigerated.

Effect of transport conditions on the stability of biochemical markers in blood.

1989 ◽  
Vol 35 (12) ◽  
pp. 2313-2316 ◽  
Author(s):  
S E Hankinson ◽  
S J London ◽  
C G Chute ◽  
R L Barbieri ◽  
L Jones ◽  
...  
Keyword(s):  
Whole Blood ◽  
Apolipoprotein B ◽  
Biochemical Markers ◽  
Room Temperature ◽  
Density Lipoprotein ◽  
Alpha Tocopherol ◽  
Endogenous Hormones ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
The Stability

Abstract We examined the stability of lipids, carotenoids, alpha-tocopherol, and endogenous hormones in plasma prepared from whole blood that had been mailed to a central location for processing. Initially, to simulate transport conditions, whole-blood samples were stored in the laboratory, either at room temperature or cooled, for up to 72 h before processing. In the latter samples, lipid concentrations changed up to 1.4% per day, carotenoids up to -5.5%, and hormones up to 9.5%. In a second study, analyte concentrations in plasma from cooled whole blood mailed via overnight courier were compared with those from plasma that had been immediately separated, frozen, and mailed via overnight courier. Concentrations of cholesterol, high-density lipoprotein subfraction 3, apolipoprotein B, and retinol were stable. Overall, for each marker except estradiol, the between-person variation was at least twice the within-person variation. In a third study, at least 340 micrograms of DNA was recovered from 30 mL of cool-shipped whole blood. Our results indicate that shipping whole-blood samples by overnight courier is feasible for assay of several biochemical markers of interest in epidemiological research.

Whole blood samples for adrenocorticotrophic hormone measurement can be stored at room temperature for 4 hours

2016 ◽  
Vol 76 (8) ◽  
pp. 653-656 ◽  
Author(s):  
Mette Christensen ◽  
Rikke Fogt Madsen ◽  
Line Rosengreen Møller ◽  
Cindy Soendersoe Knudsen ◽  
Mie Hessellund Samson
Keyword(s):  
Whole Blood ◽  
Room Temperature ◽  
Adrenocorticotrophic Hormone ◽  
Blood Samples ◽  
Whole Blood Samples

scholarly journals Comparison between Plasma and Whole Blood Specimens for Detection of Aspergillus DNA by PCR

2000 ◽  
Vol 38 (10) ◽  
pp. 3830-3833 ◽  
Author(s):  
Juergen Loeffler ◽  
Holger Hebart ◽  
Ulrike Brauchle ◽  
Ulrike Schumacher ◽  
Hermann Einsele
Keyword(s):  
Detection Limit ◽  
Whole Blood ◽  
Blood Samples ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Whole Blood Samples ◽  
Blood Specimens

Ninety-six plasma and whole blood specimens from nine selected patients were analyzed for the presence of Aspergillus DNA. Nineteen specimens from three patients with proven aspergillosis were PCR positive in both materials, whereas an additional 22 were PCR positive in whole blood only. All 36 samples from six patients without signs of aspergillosis were negative in both assays. We conclude that although plasma and whole blood spiked with Aspergillusconidia showed an identical lower detection limit (10 CFU), the sensitivity of plasma PCR was lower than that of PCR performed on whole blood samples.

scholarly journals Measurement of the alcohol biomarker phosphatidylethanol (PEth) in dried blood spots and venous blood—importance of inhibition of post-sampling formation from ethanol

2021 ◽  
Author(s):  
Olof Beck ◽  
Maria Mellring ◽  
Christian Löwbeer ◽  
Sabina Seferaj ◽  
Anders Helander
Keyword(s):  
Filter Paper ◽  
Whole Blood ◽  
Room Temperature ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Blood Samples ◽  
Ethanol Formation ◽  
Alcohol Biomarker ◽  
Blood Specimens ◽  
Blood Spot

AbstractPhosphatidylethanol (PEth) is a group of phospholipids formed in cell membranes following alcohol consumption by action of the enzyme phospholipase D (PLD). PEth measurement in whole blood samples is established as a specific alcohol biomarker with clinical and forensic applications. However, in blood specimens containing ethanol, formation of PEth may continue after sampling leading to falsely elevated concentrations. This study evaluated the use of dried blood spot (DBS) and microsampling specimens to avoid post-sampling formation of PEth. Filter paper cards and three commercial devices for volumetric microsampling of finger-pricked blood were assessed, using PEth-negative and PEth-positive whole blood fortified with 2 g/L ethanol. PEth (16:0/18:1) was measured by LC–MS/MS. Post-sampling formation of PEth occurred in wet blood and in the volumetric devices, but not filter paper cards, when stored at room temperature for 48 h. Addition of an inhibitor of PLD, sodium metavanadate (NaVO3), eliminated post-sampling formation during storage and drying. In conclusion, the present study confirmed previous observations that PEth can be formed in blood samples after collection, if the specimen contains ethanol. The results further demonstrated that post-sampling formation of PEth from ethanol also occurred with commercial devices for volumetric dried blood microsampling. In order for a PEth result not to be questioned, it is recommended to use a PLD inhibitor, whether venous blood is collected in a vacutainer tube or finger-pricked blood is obtained using devices for dried blood microsampling. Graphical abstract

scholarly journals Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
İbrahim Kaplan ◽  
Hatice Yüksel ◽  
Osman Evliyaoğlu ◽  
M. Kemal Basarali ◽  
Gülten Toprak ◽  
...  
Keyword(s):  
Cyclosporine A ◽  
Whole Blood ◽  
Room Temperature ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Blood Samples ◽  
Percent Change ◽  
Significant Difference ◽  
Whole Blood Samples ◽  
The Stability

Tacrolimus and cyclosporine A are immunosuppressant drugs with narrow therapeutic windows. The aim of this study was to investigate the stability of tacrolimus and cyclosporin A levels in whole blood samples under different storage conditions. Whole blood samples were obtained from 15 patients receiving tacrolimus and 15 patients receiving cyclosporine A. Samples were immediately analyzed and then stored at different conditions (room temperature (24°C−26°C) for 24 hours, +4°C for 24 and 48 hours, and −20°C for one month) and then analyzed again. For tacrolimus, there was a significant difference between samples analyzed immediately and those kept 24 hours at room temperature (P=0.005) (percent change 32.89%). However, there were no significant differences between the other groups. For cyclosporine A, there was a significant difference between samples analyzed immediately and those kept 24 hours (P=0.003) (percent change 19.47%) and 48 hours (P=0.002) (percent change 15.38%) at +4°C and those kept 24 hours at room temperature (P=0.011) (percent change 9.71%). Samples of tacrolimus should be analyzed immediately or stored at either +4°C or −20°C, while samples of cyclosporine A should be analyzed immediately or stored at −20°C.

The Variability of Results Between Point-of-Care Testing Glucose Meters and the Central Laboratory Analyzer

2006 ◽  
Vol 130 (10) ◽  
pp. 1527-1532
Author(s):  
Adil I. Khan ◽  
Yolanda Vasquez ◽  
Jacquelyn Gray ◽  
Frank H. Wians Jr ◽  
Martin H. Kroll
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Clinical Results ◽  
Central Laboratory ◽  
Point Of Care Testing ◽  
Blood Samples ◽  
Glucose Meter ◽  
Whole Blood Samples ◽  
Blood Specimens ◽  
Laboratory Analyzer

Abstract Context.—Point-of-care testing glucose meters are strongly recommended in the management of diabetes and are increasingly being used for making therapeutically important decisions. Thus, it is essential that their results correlate well with those of laboratory analyzers. Objectives.—To test the reliability of point-of-care testing glucose meters. Design.—Two studies were performed: (1), an in-house study comparing accuracy of point-of-care testing glucose meters with a reference analyzer using fresh whole blood specimens (2), a real-time comparison of (a) 2 successive glucose meter readings and (b) glucose meter reading to central laboratory analyzer reading. Setting.—(1), Seven glucose meters from 4 manufacturers were compared with the Yellow Springs YSI 2300 blood glucose analyzer using whole blood without preservative. (2), (a) Whole blood samples were read within 5 minutes of each other using Accu-Chek meters and (b) between a glucose meter and a Hitachi laboratory analyzer. Results.—(1) Within the Accu-Chek group of glucose meters, fresh, preservative-free whole blood samples showed the lowest bias. (2) At the hypoglycemic level, successive glucose meter readings agreed well, but there was considerable disagreement between glucose meter and central laboratory values. Because laboratory analyzers are of proven accuracy, they are used as the reference. In the glucose meter–central laboratory analyzer correlation, for both hypoglycemic and hyperglycemic values, readings in which the differences were greater than 10% occurred more than 61% of the time. In the hypoglycemic range, differences greater than 20% occurred 57% of the time. Conclusions.—One should scrutinize point-of-care testing glucose meter readings at the hypoglycemic and hyperglycemic levels and whenever possible to corroborate these clinical results with central laboratory analyzers.

scholarly journals Extended storage of whole blood with 3-deazaadenosine for homocysteine assay

2007 ◽  
Vol 44 (4) ◽  
pp. 388-390
Author(s):  
Monica Hansrani ◽  
Gerard Stansby
Keyword(s):  
Whole Blood ◽  
Room Temperature ◽  
Statistical Difference ◽  
Plasma Homocysteine ◽  
Blood Samples ◽  
Time Points ◽  
Whole Blood Samples ◽  
Significant Change

Background: To assess the effectiveness of using 3-deazaadenosine (3DAA) to maintain plasma homocysteine concentrations (tHCy) in whole blood samples. Methods: Blood was obtained from five volunteers and samples were maintained at room temperature, in cold packs or in a fridge (0-4°C) with and without 3DAA. At time points ranging from 6 to 168 h, samples were processed and analysed for tHCy using the Abbott IMx system. Results: There was a mean increase in tHCy of 29.4% at 6 h increasing to 242.6% after 168 h in whole blood kept at room temperature. There was no significant change in tHCy for 48 h when stored in cold packs, and for 72 h when stored in the fridge. The addition of 3DAA had a significant preservative effect ( P<0.001), maintaining tHCy to 48 h in whole blood at room temperature, 120 h in the fridge and 96 h in cool packs. There was no statistical difference in results obtained from samples containing preservative and controls when using the Abbott IMx system. Conclusion: 3DAA is an effective preservative of tHCy in whole blood, particularly in samples maintained at 0-4°C.

Steadiness/stability of antiepileptic drugs in serum, plasma and whole-blood samples under various storage methods

2010 ◽  
Vol 41 (02) ◽  
Author(s):  
N Shazi ◽  
A Böss ◽  
HJ Merkel ◽  
F Scharbert ◽  
D Hannak ◽  
...  
Keyword(s):  
Antiepileptic Drugs ◽  
Whole Blood ◽  
Blood Samples ◽  
Storage Methods ◽  
Whole Blood Samples
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