scholarly journals Effect of serum-clot contact time on clinical chemistry laboratory results

1998 ◽  
Vol 44 (6) ◽  
pp. 1325-1333 ◽  
Author(s):  
Dongbo J Zhang ◽  
R K Elswick ◽  
W Greg Miller ◽  
Jimmy L Bailey
Keyword(s):  
Contact Time ◽  
Clinical Chemistry ◽  
Hdl Cholesterol ◽  
The Other ◽  
Chemistry Laboratory ◽  
Blood Collection ◽  
Laboratory Results ◽  
Clinical Chemistry Laboratory ◽  
The Stability ◽  
Blood Collection Tubes

Abstract The effect of serum-clot contact time on laboratory results was studied by dividing each blood specimen into four blood collection tubes. The control sera were separated from the clot within 30 min of the collection. The other tubes were incubated at 32 °C, and the sera were separated at 3, 6, and 24 h. The sera were stored at 4 °C and analyzed at the same time. The stability of the tests was determined by comparing the results of the 3-, 6-, and 24-h samples with the values from the 30-min samples. The acceptable limits around the 30-min values were derived from the analytical and intraindividual biological variation of the tests. A total of 63 analytes were studied. Potassium, phosphorous, and glucose were the least stable, and the serum should be separated from the clot within 3 h for these analytes. Albumin, bicarbonate, chloride, C-peptide, HDL-cholesterol, iron, LDL-cholesterol, and total protein should be separated within 6 h. The other analytes were stable for 24 h of serum-clot contact.

Plasma alkaline phosphatase assay: interconversion of results by two methods.

1977 ◽  
Vol 23 (11) ◽  
pp. 2148-2150 ◽  
Author(s):  
R H Eaton
Keyword(s):  
Alkaline Phosphatase ◽  
Clinical Chemistry ◽  
The Other ◽  
Chemistry Laboratory ◽  
Bicarbonate Buffer ◽  
Rate Analysis ◽  
Alkaline Phosphatase Assay ◽  
Wide Range ◽  
Clinical Chemistry Laboratory ◽  
Plasma Alkaline Phosphatase

Abstract Two methods for measuring plasma alkaline phosphatase activity are compared: one makes use of phenyl phosphate, carbonate-bicarbonate buffer, and continuous-flow methodology; the other of p-nitrophenyl phosphate, diethanolamine buffer, and reaction-rate analysis. Results by the methods correlate well (r = 0.98) over a wide range of values (up to 10-fold the upper limit of normal). A factor can therefore be applied to convert results by one method into those that would be obtained by the other. The possibility that the presence of different proportions of isoenzymes in the plasma will affect this factor is considered. We have used the new method, with a conversion factor, as the routine method of alkaline phosphatase measurement in a clinical chemistry laboratory, with no problems.

scholarly journals Quality Indicators for Evaluating Errors in the Preanalytical Phase

2021 ◽  
Author(s):  
Mohit Mehndiratta ◽  
Eram Hussain Pasha ◽  
Nilesh Chandra ◽  
Edelbert Anthonio Almeida
Keyword(s):  
Quality Indicators ◽  
Clinical Laboratory ◽  
Clinical Chemistry ◽  
Tertiary Care ◽  
Chemistry Laboratory ◽  
Blood Collection ◽  
Care Hospital ◽  
Preanalytical Phase ◽  
Tertiary Center ◽  
Clinical Chemistry Laboratory

Abstract Objective The aim of this study was to study the incidence of preanalytical errors in the clinical chemistry laboratory attached to a tertiary care hospital. Design and Methods The study was conducted in a clinical chemistry laboratory using the samples and forms received for analysis. Five hundred random samples were analyzed using a predefined set of quality indicators (QIs) over a period of 3 months. The incidence of each preanalytical error was described as a percentage of the total samples analyzed in the study. Statistical Analysis Individual QIs were assigned values as 0 and 1 and were used to assess each sample; 0 if the error was present, and 1 if absent. The incidence of each preanalytical error was described as a percentage of the total samples analyzed in the study. Result Out of the 500 samples observed, 138 samples were error free, while 21 samples had the maximum number of errors, that is, 6. The error committed most often was the omission of provisional diagnosis being mentioned on the requisition form. No preanalytical error was observed for QIs: selecting the appropriate blood collection vial or storage of sample. Conclusion This study confirms that error rate in the preanalytical phase is high and vastly ignored. Errors committed here may be overlooked, given the large number of samples received in the clinical laboratory of a tertiary center. To reduce these errors, the laboratory should provide training to all workers involved in the preanalytical phase. Daily or weekly QI scores should be recorded to assess and rectify shortcomings, thereby improving patient care.

A Punched-Card System for the Laboratory

1962 ◽  
Vol 8 (5) ◽  
pp. 538-545 ◽  
Author(s):  
Nathan Radin
Keyword(s):  
Clinical Chemistry ◽  
Laboratory Data ◽  
Chemistry Laboratory ◽  
Test Card ◽  
Clinical Chemistry Laboratory ◽  
Original Laboratory ◽  
Numerical Order ◽  
Card System ◽  
A Charge ◽  
Laboratory Record

Abstract A punched-card system was developed to simplify the administrative aspects of the clinical chemistry laboratory. In it a three-part requisition and samples are matched and given a laboratory number. The third copy of the original laboratory requsition is a charge card also used as a name file for the day. Prepunched cards, coded for laboratory tests, are used for each test requested. The laboratory number is stamped and punched on each test card. All test cards are then sorted into laboratory test groups, after which the laboratory data and results are recorded on them. Cards are then sorted for laboratory number and rematched with the original laboratory requisitions, which remained in numerical order. Results are then copied directly onto the requisitions. The system is more efficient, less susceptible to error, and simpler than other laboratory record systems.

Simulation of a clinical chemistry laboratory

1983 ◽  
Vol 14 (3) ◽  
pp. 301-316
Author(s):  
ARVIND K. N. NANDEDKAR ◽  
A. BLANK ◽  
JACOB ROOTENBERG
Keyword(s):  
Clinical Chemistry ◽  
Chemistry Laboratory ◽  
Clinical Chemistry Laboratory

Hyperalimentation Laboratory Support

PEDIATRICS ◽  
1982 ◽  
Vol 69 (6) ◽  
pp. 833-833
Author(s):  
Joe Rutledge ◽  
Larry Miller
Keyword(s):  
Pediatric Patients ◽  
Clinical Chemistry ◽  
Serum Triglyceride ◽  
Chemistry Laboratory ◽  
Therapeutic Monitoring ◽  
Fat Emulsion ◽  
Serum Fatty Acid ◽  
American Academy Of Pediatrics ◽  
Clinical Chemistry Laboratory ◽  
Intravenous Fat Emulsions

The Committee on Nutrition, American Academy of Pediatrics has recently reported on the "Use of Intravenous Fat Emulsions in Pediatric Patients" (Pediatrics 68:738, 1981). The committee recommends restriction of the amount of fat emulsion given infants because of a lack of accurate and appropriate means of monitoring serum fatty acid and triglyceride concentrations. Such is not the case for triglycerides. The pediatric clinical chemistry laboratory has the ability to measure serum triglyceride concentrations to support hyperalimentation therapeutic monitoring.

A Pilot Study on the Evaluation of Clinical Chemistry Laboratory Test Performance using Six Sigma Metrics

2019 ◽  
Vol 4 (2) ◽  
pp. 31-36
Author(s):  
Pier Angeli Medina ◽  
◽  
Jenny Matibag ◽  
Sarah Jane Datay-Lim ◽  
Elizabeth Arcellana-Nuqui
Keyword(s):  
Pilot Study ◽  
Laboratory Test ◽  
Test Performance ◽  
Six Sigma ◽  
Clinical Chemistry ◽  
Chemistry Laboratory ◽  
Clinical Chemistry Laboratory
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