scholarly journals Interspecies variation in DNA damage induced by pollution

2014 ◽  
Vol 60 (2) ◽  
pp. 308-321 ◽  
Author(s):  
Claudia Sebbio ◽  
Claudio Carere ◽  
Giuseppe Nascetti ◽  
Bruno Bellisario ◽  
Pasquale Mosesso ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Power Plant ◽  
Comet Assay ◽  
Tissue Concentration ◽  
Central Italy ◽  
Rana Esculenta ◽  
Chemical Pollution ◽  
Aphanius Fasciatus ◽  
The Impact

Abstract The choice of a suitable species to translate pollution signals into a quantitative monitor is a fundamental step in biomonitoring plans. Here we present the results of three years of biomonitoring at a new coal power plant in central Italy using three different aquatic and terrestrial wildlife species in order to compare their reliability as sentinel organisms for genotoxicity. The comet assay was applied to the common land snail Helix spp., the lagoon fish Aphanius fasciatus, and the green frog Rana esculenta sampled in the area potentially exposed to the impact of the power station. The tissue concentration of some expected pollutants (As, Cd, Ni, Pb, Cr) was analysed in parallel samples collected in the same sampling sites. The three species showed different values in the comet assay (Tail Intensity) and different accumulation profiles of heavy metals. Aphanius fasciatus showed an increasing genotoxic effect over time that paralleled the temporal increase of the heavy metals, especially arsenic, and the highest correlation between heavy metals and DNA damage. Helix spp. showed levels of damage inversely related to the distance from the source of pollution and in partial accordance with the total accumulation of trace elements. On the contrary, Rana esculenta showed a low capability to accumulate metals and had inconsistent results in the comet test. The fish appeared to be the most efficient and sensitive species in detecting chemical pollution. Overall, both the fish and the snail reflected a trend of increasing pollution in the area surrounding the power plant across time and space.

Sodium-iodide symporter positive cells after intracellular uptake of 99mTc versus α-emitter 211At

2012 ◽  
Vol 51 (05) ◽  
pp. 170-178 ◽  
Author(s):  
M. Wendisch ◽  
R. Freudenberg ◽  
R. Runge ◽  
L. Oehme ◽  
G.J. Meyer ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Cell Model ◽  
Absorbed Dose ◽  
Thyroid Cell ◽  
Intracellular Uptake ◽  
Sodium Iodide Symporter ◽  
Radionuclide Distribution ◽  
Rat Thyroid ◽  
The Impact

SummaryPurpose: We evaluated the DNA damaging potential of Auger electrons emitted in the decay of 99mTc compared to α-particles of 211At. Material and methods: The impact of 99mTc and 211At was monitored in a NIS-expressing rat thyroid cell model PC Cl3 with varying, yet defined intra- and extracellular radionuclide distribution (using ± perchlorate). The radiotoxicity of 99mTc and 211At was studied by the comet assay under neutral and alkaline conditions and colony formation. Results: In the presence of perchlorate, the radioactivity yielding 37 % cellular survival, A37, was estimated to be (0.27 ± 0.02) MBq/ml and (450 ± 30) MBq/ml for 211At and 99mTc, respectively. In absence of perchlorate, cellular radiotracer uptake was similar for both radionuclides (2.2 %, 2.7 %), yet the A37 was reduced by 82% for the α-emitter and by 95 % for 99mTc. Cellular dose increased by a factor of 5 (211At) and 38 (99mTc). Comet assays revealed an increased DNA damage after intracellular uptake of both radiotracers. Conclusions: The data indicate damage to the cell to occur from absorbed dose without recognizable contribution from intracellular heterogeneity of radionuclide distribution. Comet assay under alkaline and neutral conditions did not reveal any shift to more complex DNA damage after radionuclide uptake. Cellular uptake of 99mTc and 211At increased cellular dose and reduced clonogenic survival.

Comet Assay: A Strong Tool for Evaluating DNA Damage and Comprehensive Guidelines for Plant Cells

2017 ◽  
Vol 3 (02) ◽  
pp. 65-72
Author(s):  
Ashish Agnihotri ◽  
Chandra Shekhar Seth
Keyword(s):  
Heavy Metals ◽  
Dna Damage ◽  
Comet Assay ◽  
Single Cells ◽  
Strand Breakage ◽  
Dna Strand ◽  
Environmental Genotoxicity ◽  
Genetic Ecotoxicology ◽  
Detailed Protocol ◽  
Dna Strand Breakage

Heavy metals affect plant system in various toxic ways including morphophysiological alterations and genotoxic damages inside a plant cell. The extent of DNA damage under any genotoxic agents can be effectively measured in single cells applying comet assay approach. Comet assay primarily measures DNA strand breakage in single cells and its use has increased in different areas: clinical applications, human monitoring, radiation biology and genetic ecotoxicology. This paper is a review of the detailed protocol and precautions to be taken while performing comet assay and may have been slightly modified from other original protocols according to the plant, organ, cell type, etc. In conclusion, the study reviewed in this paper demonstrate that the comet assay application in plants provides a reliable, sensitive and rapid system for the study of environmental genotoxicity caused by heavy metals.

scholarly journals Ecological evaluation of the urban soil in Perm

2021 ◽  
Vol 100 (2) ◽  
pp. 116-122
Author(s):  
Irina L. Maslennikova ◽  
Mihail A. Shishkin ◽  
Natal’ya P. Sherstobitova ◽  
Marina V. Kuznetsova
Keyword(s):  
Heavy Metals ◽  
Environmental Assessment ◽  
Urban Soils ◽  
Soil Analysis ◽  
Water Soluble ◽  
Pollution Monitoring ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Pollution ◽  
Moderate Degree ◽  
The Impact

Introduction. Representations of the impact of anthropogenesis processes on the soil’s ecological state are the basis for improvement of the environmental pollution monitoring system to make correct architectural and planning decisions. Material and methods. During 2016-2018 an environmental assessment of pollution of 214 samples of urban soils was performed. The soil analysis was carried out according to a random sample as one test site per 1 km². The concentration of heavy metals was determined by atomic absorption spectrometry. The analysis of organic compounds was carried out by gas chromatography-mass spectrometry. The total number of heterotrophic and gram-negative bacteria of the Enterobacteriaceae family was determined by direct seeding on selective media. Statistical data processing was carried out using software Statistica. Results. The level of chemical contamination with water-soluble forms of heavy metals of Perm soils was assessed. The concentration of heavy metals Cu, Pb, Zn, Fe, Cd, Ni, Mn in the control samples of sandy/loamy soils was 0.09/0.15; 0.08/0.22; 0.22/0.39; 5.57/16.14; 0.02/0.01; 0.06/0.13; 0.74/3.14 mg/l, respectively. The values of the total pollution index (Zc) above 16 were recorded at 4.7% of the soil samples, which corresponds to a moderate degree of pollution. It was shown that the soil type influenced Zc (KW-H (3; 214) = 30.73; p = 0.00001) and amounted to 6.24 ± 3.72 for loamy soils; for peat - 22.42 ± 12.32; for sand - 9.23 ± 5.70; for old urban soils - 8.37 ± 4.07. The median of the heterotroph bacteria content in the soils for I (Zc: 0-4) and IV (Zc ≤ 16) groups was revealed to correspond to the categories of «poor,» II (Zc: 4-8) and III (Zc: 8-16) groups - «inferior» soil. A correlation between the content of heterotrophs and the number of bacteria E. coli was not detected. According to Zc, for each soil group, no statistical differences in phthalate content were noted. Conclusion. Environmental assessment of soils should be comprehensive, as the types of anthropogenic chemical pollution, that have varying degrees of correlations between each other, affect quantitative changes in soil microbiota, which in the future may determine the ability to maintain homeostasis in this ecosystem.

APPLICATION OF MOSS AS BIO-MONITOR FOR HEAVY METALS DEPOSITION IN PORT DICKSON, MALAYSIA

2016 ◽  
Vol 78 (6-6) ◽  
Author(s):  
Ahmad Saat ◽  
Nurul Aida Mohamed ◽  
Zaini Hamzah ◽  
Suhaimi Hamzah
Keyword(s):  
Heavy Metals ◽  
Power Plant ◽  
Point Sources ◽  
Elevated Concentration ◽  
Road Vehicles ◽  
Naa Technique ◽  
Local Residents ◽  
Public Holidays ◽  
The Impact ◽  
The Town

Port Dickson in Malaysia is a sea-side township of mixed activities within the area, comprising of a coal-fired power plant, a petroleum refinery, a gas-fuelled power plant, and tourism with the visit of many road vehicles, especially during weekends and public holidays. The impact of emission from these activities to the environment has becomes a matter of concern to the authorities as well as local residents.  The present study embarked on the use of mosses (Octoblepharum sp. & Isopterygium sp.) as long terms monitor of heavy metals emission from such activities. Mosses samples were collected on land area within 30 km radius from the town centre. Prepared samples were analysed for the concentration of As, U, Th, Fe, Cr and Zn using Neutron Activation Analysis (NAA) technique. These metals are related to various industries, fuel combustion and refineries emissions. Although the results show no elevated concentration of the elements studied, except for Cr, the spatial pattern of heavy metal distributions observed reflected the energy-related activities and vehicular emissions in the study area. Enrichment Factor values of each element also in agreement with the distribution pattern. The research finding suggests that ‘moss-method’ is applicable for deposition measurement in vicinity of stationary as well as non-point sources.

scholarly journals Accumulation of Heavy Metals by Soil and Agricultural Plants in the Zone of Technogenic Impact

2021 ◽  
Author(s):  
D.V. Vinogradov ◽  
T.V. Zubkova
Keyword(s):  
Heavy Metals ◽  
Power Plant ◽  
Climatic Conditions ◽  
Impact Zone ◽  
Mobile Forms ◽  
Area Sampling ◽  
Zone Of Influence ◽  
Agricultural Plants ◽  
Industrial Regions ◽  
The Impact

Background: The transformation of natural elements, the migration of soil substances used in industry most often refer to metals, as a result of which “metallization” of landscapes occurs in industrial regions. Methods: The article proposes an analysis of studies, conducting agroecological monitoring of soil and plants in the zone affected by the action of Ryazan power plant in 2015-2019. Experiments included the analysis of the soil and climatic conditions of the area, sampling in the zone of five rings of the pipe of the polluting enterprise, the analysis of the content of some trace elements in the coal during combustion and the products of their combustion, as well as heavy metals in the biomass of the main types of plant communities in the zone of influence and mobile forms of metals in the soil of the zone, depending on the main directions of influence. Result: According to the results, the greatest distribution of mobile forms of heavy metals in the impact zone of Ryazan State District Power Plant (RGRES) was observed in the southern and southeastern directions in radii from 2.5 km to 30 km, in the southwest direction it was in radii from 500 m to 7 km and in the northern and northwestern directions it was within a radius of 500 m to 2.5 km from the station.

scholarly journals Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1930
Author(s):  
Vanessa Valdiglesias ◽  
Natalia Fernández-Bertólez ◽  
Carlota Lema-Arranz ◽  
Raquel Rodríguez-Fernández ◽  
Eduardo Pásaro ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Metal Oxide ◽  
Oxidative Dna Damage ◽  
Metal Oxide Nanoparticles ◽  
Consumer Products ◽  
Specific Regulation ◽  
Vitro Model ◽  
The Impact

Metal oxide nanoparticles (NPs) have a wide variety of applications in many consumer products and biomedical practices. As a result, human exposure to these nanomaterials is highly frequent, becoming an issue of concern to public health. Recently, human salivary leucocytes have been proposed as an adequate non-invasive alternative to peripheral blood leucocytes to evaluate genotoxicity in vitro. The present study focused on proving the suitability of salivary leucocytes as a biomatrix in the comet assay for in vitro nanogenotoxicity studies, by testing some of the metal oxide NPs most frequently present in consumer products, namely, titanium dioxide (TiO2), zinc oxide (ZnO), and cerium dioxide (CeO2) NPs. Primary and oxidative DNA damage were evaluated by alkaline and hOGG1-modified comet assay, respectively. Any possible interference of the NPs with the methodological procedure or the hOGG1 activity was addressed before performing genotoxicity evaluation. Results obtained showed an increase of both primary and oxidative damage after NPs treatments. These data support the use of salivary leucocytes as a proper and sensitive biological sample for in vitro nanogenotoxicity studies, and contribute to increase the knowledge on the impact of metal oxide NPs on human health, reinforcing the need for a specific regulation of the nanomaterials use.

Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

1999 ◽  
Vol 96 (1) ◽  
pp. 143-146 ◽  
Author(s):  
J.-P. Pouget ◽  
J.-L. Ravanat ◽  
T. Douki ◽  
M.-J. Richard ◽  
J. Cadet
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Gamma Radiation ◽  
In Cells

Агрономическая эффективность промышленной (голландской) технологии возделывания картофеля

2018 ◽  
Author(s):  
C. Coy ◽  
A.V. Shuravilin ◽  
O.A. Zakharova
Keyword(s):  
Heavy Metals ◽  
Regression Analysis ◽  
Positive Response ◽  
Growth Yield ◽  
Correlation And Regression Analysis ◽  
Yield And Quality ◽  
Quality Of Products ◽  
High Degree ◽  
The Impact

Приведены результаты исследований по изучению влияния промышленной технологии возделывания картофеля на развитие, урожайность и качество продукции. Выявлена положительная реакция растений на подкормку K2SO4 в период посадки. Корреляционно-регрессионный анализ урожайности и качества клубней выявил высокую степень достоверности результатов опыта. Содержание нитратов и тяжелых металлов в клубнях было ниже допустимых величин.The results of studies on the impact of industrial technology of potato cultivation on growth, yield and quality of products. There was a positive response of plants to fertilizer K2SO4 in the period of planting. Correlation and regression analysis of yield and quality of tubers revealed a high degree of reliability of the results of experience. The contents of nitrates and heavy metals in tubers was below the permissible values.

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