scholarly journals Inhibition of Human Cervical Cancer Cell Growth by Ethanolic Extract ofBoerhaavia diffusaLinn. (Punarnava) Root

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Rakhi Srivastava ◽  
Daman Saluja ◽  
Bilikere S. Dwarakanath ◽  
Madhu Chopra
Keyword(s):  
Cell Cycle ◽  
Hela Cells ◽  
Morphological Changes ◽  
Ethanolic Extract ◽  
Antiproliferative Effect ◽  
Chloroform Fraction ◽  
Anticancer Activities ◽  
Cancer Cell Growth ◽  
Human Cervical Cancer Cell ◽  
Inhibition Of Dna Synthesis

In Indian traditional medicine,Boerhaavia diffusa(punarnava) roots have been widely used for the treatment of dyspepsia, jaundice, enlargement of spleen, abdominal pain and as an anti-stress agent. Pharmacological evaluation of the crude ethanolic extract ofB. diffusaroots has been shown to possess antiproliferative and immunomodulatory properties. The extract ofB. diffusawas studied for anti-proliferative effects on the growth of HeLa cells and for its effect on cell cycle. Bio-assays of extracts fromB. diffusaroot showed that a methanol : chloroform fraction (BDF 5) had an antiproliferative effect on HeLa cells. After 48 h of exposure, this fraction at a concentration of 200 μg mL−1significantly reduced cell proliferation with visible morphological changes in HeLa cells. Cell cycle analysis suggests that antiproliferative effect of BDF 5 could be due to inhibition of DNA synthesis in S-phase of cell cycle in HeLa cells, whereas no significant change in cell cycle was detected in control cells. The fraction BDF 5 caused cell death via apoptosis as evident from DNA fragmentation and caspase-9 activation. Thus the extract has potential to be evaluated in detail to assess the molecular mechanism-mediated anticancer activities of this plant.

scholarly journals Anticancer and Antioxidant Activities of Some Algae from Western Libyan Coast

2016 ◽  
Author(s):  
Rabia Alghazeer ◽  
Mahboba Enaeli ◽  
Nazlin K. Howell
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Antioxidant Activity ◽  
Molecular Mechanisms ◽  
Antioxidant Activities ◽  
Morphological Changes ◽  
Reducing Power ◽  
Bioactive Metabolites ◽  
Anticancer Activities ◽  
Hypnea Musciformis

Seaweeds are considered as one of the largest biomass producers in marine environment that is rich in bioactive metabolites and a source of natural ingredients for functional foods. The potential antioxidant activity and the potential inhibition of Caco2 cell proliferation, of crude extracts of: Chlorophyta (Ulva lactuca, and Codium tomentosum), Phaeophyta (Cystoseira crinita, Cystoseira stricta, and Sargassum vulgare), and Rhodophyta (Gelidium latifolium, Hypnea musciformis, and Jania rubens) collected from western Libyan coast were evaluated in vitro. The antioxidant activity was determined by reducing power and DPPH assays while cell proliferation, morphological changes and the cell cycle arrest were assessed by MTT, inverted light microscope and flow cytometry methods respectively. The polyphenols and flavonoids rich extracts showed remarkable reducing power and antiradical properties. After exposure of Caco2 cells to; various concentrations of extracts (50, 100,150 and 200 µg/mL) especially from brown algae for 72 h, significantly reduced cell proliferation. The antiproliferative effect of algae extracts was correlated with their polyphenol and flavonoid contents. Cell cycle analysis further showed that cells were arrested in G phases along with an increment in sub-diploidal cell population (sub-G) after extract application. These results imply that seaweeds which are rich in bioactive compounds may be in anticancer drug research programs. However, further investigations are essential to reveal the molecular mechanisms of the anticancer activities of these algae.

scholarly journals Ethanol Extract of Croton Kongensis Promotes Cell Cycle Arrest and Consequently Inhibits Anchorage-Independent Growth of Cervical Cancer Hela Cells

2021 ◽  
Vol 37 (3) ◽  
Author(s):  
Nguyen Thi Bich Loan ◽  
Nguyen Lai Thanh ◽  
Pierre Duez ◽  
Nguyen Dinh Thang
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Cell Cycle Arrest ◽  
Hela Cells ◽  
Ethanol Extract ◽  
Soft Agar ◽  
Anticancer Activities ◽  
Anchorage Independent Growth ◽  
Cycle Arrest ◽  
G2 Phase

Extracts from Croton kongenis present anticancer activities on various cancers. However, there is no research conducted to investigate the effects of Croton kongenis extracts on cervical cancer as well as on zebrafish. In this study, we demonstrated that Croton kongenis ethanol extract expressed high toxicity to cervical cancer Hela cells with an IC50 dose of 20.4 µg/mL and to zebrafish embryos with malformations, lethality and hatching inhibition at 72-hpf at effective dose of 125 µg/mL. Interestingly, treatment with Croton kongenis ethanol extract caused cell-cycle-arrest at the G2 phase. Particularly, percentages of Croton kongenis ethanol extract-treated cells in G1, S, G2/M were 70%, 6% and 23%, while percentages of control cells in G1, S, G2/M were 65%, 15% and 18%, respectively. Consistent with cell-cycle-arrest, the expressions of CDKN1A, CDNK2A and p53 in Croton kongenis ethanol extract-treated cells were up-regulated 2.0-, 1.65- and 1.8-fold, respectively. Significantly, treatment with Croton kongenis ethanol extract inhibited anchorage-independent growth of Hela cells; the number of colonies formed in soft-agar of Croton kongenis ethanol extract-treated cells was only one-fourth of that of control cells. In conclusion, we suggest that Croton kongenis ethanol extract could be able to use as a traditional medicine for treatment of cervical cancer.

scholarly journals Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Leilei Xu ◽  
Qin Zhang ◽  
Changhua Li ◽  
Fu Hua ◽  
Xiaoping Liu
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Hela Cell ◽  
Hela Cells ◽  
Target Gene ◽  
Transfection Efficiency ◽  
Inhibitory Effect ◽  
Migration And Invasion ◽  
Cancer Cell Growth ◽  
Better Than

The application of ultrasound and microbubbles (USMB-) mediated microRNA (miR) is a promising approach of gene delivery for cancer treatment. We aimed to discuss the effects of USMB-miR-505 on cervical cancer (CC) development. miR-505 mediated by USMB was prepared. The effect of miR-505 on its transfection efficiency and the effect of miR-505 on HeLa cell proliferation, cell cycle, apoptosis, migration, and invasion were studied. The target gene of miR-505 was predicted, and its expression in CC was detected. The effect of the target gene on HeLa cells was further verified. USMB-miR-505 showed a higher transfection efficiency than miR-505 alone. The inhibitory effect of miR-505 mediated by USMB on HeLa cells was better than miR-505. miR-505 targeted AKT2, which was upregulated in CC. Overexpression of AKT2 reversed the inhibitory effect of USMB-miR-505 on HeLa cell malignant behaviors. Overall, we highlighted that USMB-miR-505 inhibited HeLa cell malignant behaviors by targeting AKT2.

scholarly journals In vitro assessment of anti-proliferative effect induced by α-mangostin from Cratoxylum arborescens on HeLa cells

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3460 ◽  
Author(s):  
Aisha I. El habbash ◽  
Najihah Mohd Hashim ◽  
Mohamed Yousif Ibrahim ◽  
Maizatulakmal Yahayu ◽  
Fatima Abd Elmutaal Omer ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Hela Cells ◽  
Morphological Changes ◽  
Cell Permeability ◽  
Phase Cell ◽  
Dependent Manner ◽  
Ros Production ◽  
Chemical Structures

Natural medicinal products possess diverse chemical structures and have been an essential source for drug discovery. Therefore, in this study, α-mangostin (AM) is a plant-derived compound was investigated for the apoptotic effect on human cervical cancer cells (HeLa). The cytotoxic effects of AM on the viability of HeLa and human normal ovarian cell line (SV40) were evaluated by using MTT assay. Results showed that AM inhibited HeLa cells viability at concentration- and time-dependent manner with IC50 value of 24.53 ± 1.48 µM at 24 h. The apoptogenic effects of AM on HeLa were assessed using fluorescence microscopy analysis. The effect of AM on cell proliferation was also studied through clonogenic assay. ROS production evaluation, flow cytometry (cell cycle) analysis, caspases 3/7, 8, and 9 assessment and multiple cytotoxicity assays were conducted to determine the mechanism of cell apoptosis. This was associated with G2/M phase cell cycle arrest and elevation in ROS production. AM induced mitochondrial apoptosis which was confirmed based on the significant increase in the levels of caspases 3/7 and 9 in a dose-dependent manner. Furthermore, the MMP disruption and increased cell permeability, concurrent with cytochrome c release from the mitochondria to the cytosol provided evidence that AM can induce apoptosis via mitochondrial-dependent pathway. AM exerted a remarkable antitumor effect and induced characteristic apoptogenic morphological changes on HeLa cells, which indicates the occurrence of cell death. This study reveals that AM could be a potential antitumor compound on cervical cancer in vitro and can be considered for further cervical cancer preclinical and in vivo testing.

scholarly journals Ethyl Acetate Fraction of Caesalpinia sappan L. Enhances Cisplatin’s Cytotoxicity on HeLa Cells via G1 and S Arrest through p53 Expression

2017 ◽  
Vol 8 (2) ◽  
pp. 51 ◽  
Author(s):  
Ulfatul Husnaa ◽  
Ni Putu Linda Laksmiani ◽  
Ratna Asmah Susidarti ◽  
Edy Meiyanto
Keyword(s):  
Cell Cycle ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Hela Cells ◽  
Morphological Changes ◽  
Ethyl Acetate Fraction ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
P53 Expression ◽  
Cycle Arrest

Cisplatin (cisp) is the first line chemotherapeutic agent for several cancer diseases which can cause significant side effects and cellular resistance. Combination-chemotherapy treatment (co-chemotherapy) was reported to be able to reduce cisp effects. Therefore, this study was carried out to investigate the cytotoxic activity of ethyl acetate fraction of C. sappan (EFC) in combination with cisp by observing apoptosis induction and cell cycle profile. Cytotoxic activity was evaluated by MTT assay. Cell cycle and apoptosis analysis were performed using flow cytometry and p53 expression was analyzed using immunocytochemistry. EFC performed cytotoxic effect on HeLa cells by showing morphological changes such as cell shrinkage, rounding and decreasing of cells viability in concentration dependent manner, giving IC50 value of 65 μg/mL. Combination of EFC and cisp in low concentration decreased cell viability into 36.86%. Further assay indicated that this combination caused redistribution of cell cycle arrest in G1 and S phases through p53 stabilization in nucleus. However, that mechanism was not followed by apoptosis. These results provide evidence to support EFC development as the enhancer of cisp effect, by improving its cytotoxicity on HeLa cells. EFC increases HeLa cells sensitivity to cisp through G1 and S cells’ arrest depending on p53 expression. Key words: co-chemotherapy, EFC, cervix cancer HeLa cells, p53, G1 and S arrest.         

APOPTOSIS INDUCTION OF ETHANOLIC EXTRACT OF AMOMUM SUBULATUM ROXB. IN HELA CELL LINES

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (03) ◽  
pp. 41-47
Author(s):  
Prakash Ramakrishnan ◽  
◽  
Ramya Neducheziyean ◽  
Keyword(s):  
Hela Cell ◽  
Dna Fragmentation ◽  
Hela Cells ◽  
Nuclear Dna ◽  
Morphological Changes ◽  
Ethanolic Extract ◽  
Hela Cell Lines ◽  
Ic50 Value ◽  
Study Results ◽  
Amomum Subulatum

Deficiency in apoptosis is one of the key factors that plays a pivotal role in cancer cell growth and proliferation. A procedure used in the treatment of cancer is the triggering of apoptosis in cancer cells. The current study aims to investigate the anticancer property of ethanolic extract of Amomum subulatum Roxb. against HeLa cell line. The MTT 3-(4, 5-di-methylthiazol-2-yl)-2,5-diphenyl-2H tetrazolium bromide assay is the quantitative cytotoxicity assay used, maximal inhibitory concentration (IC50) value was selected as the cytotoxicity parameter. The IC50 value of A. subulatum Roxb. extract is 62.5 μg/ mL and for 5-fluorouracil it is 7.8 μg/mL which indicates anticarcinogenic properties against HeLa cells. The apoptotic morphological changes in HeLa cells were observed using an inverted microscope and changes in HeLa cells were noticed after treatment with 62.5 mg/mL of ethanolic extracts, followed by acridine orange and ethidium bromide staining. The induction of apoptosis by extract of A. subulatum Roxb. was determined using the DNA fragmentation study. The results of the DNA fragmentation study, which exhibits prototypical intrinsic apoptotic characterization, also included fragmentation of nuclear DNA. We also found that the expression of Bcl2 and p53 mRNA were measured using RT-PCR. Overall, the current study results suggest that the

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