APOPTOSIS INDUCTION OF ETHANOLIC EXTRACT OF AMOMUM SUBULATUM ROXB. IN HELA CELL LINES

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (03) ◽  
pp. 41-47
Author(s):  
Prakash Ramakrishnan ◽  
◽  
Ramya Neducheziyean ◽  
Keyword(s):  
Hela Cell ◽  
Dna Fragmentation ◽  
Hela Cells ◽  
Nuclear Dna ◽  
Morphological Changes ◽  
Ethanolic Extract ◽  
Hela Cell Lines ◽  
Ic50 Value ◽  
Study Results ◽  
Amomum Subulatum

Deficiency in apoptosis is one of the key factors that plays a pivotal role in cancer cell growth and proliferation. A procedure used in the treatment of cancer is the triggering of apoptosis in cancer cells. The current study aims to investigate the anticancer property of ethanolic extract of Amomum subulatum Roxb. against HeLa cell line. The MTT 3-(4, 5-di-methylthiazol-2-yl)-2,5-diphenyl-2H tetrazolium bromide assay is the quantitative cytotoxicity assay used, maximal inhibitory concentration (IC50) value was selected as the cytotoxicity parameter. The IC50 value of A. subulatum Roxb. extract is 62.5 μg/ mL and for 5-fluorouracil it is 7.8 μg/mL which indicates anticarcinogenic properties against HeLa cells. The apoptotic morphological changes in HeLa cells were observed using an inverted microscope and changes in HeLa cells were noticed after treatment with 62.5 mg/mL of ethanolic extracts, followed by acridine orange and ethidium bromide staining. The induction of apoptosis by extract of A. subulatum Roxb. was determined using the DNA fragmentation study. The results of the DNA fragmentation study, which exhibits prototypical intrinsic apoptotic characterization, also included fragmentation of nuclear DNA. We also found that the expression of Bcl2 and p53 mRNA were measured using RT-PCR. Overall, the current study results suggest that the

scholarly journals Antiproliferative effects of some medicinal plants on HeLa cells

2013 ◽  
Vol 65 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Desanka Cenic-Milosevic ◽  
Z. Tambur ◽  
D. Bokonjic ◽  
S. Ivancajic ◽  
Tatjana Stanojkovic ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Hela Cells ◽  
Melissa Officinalis ◽  
Echinacea Angustifolia ◽  
Antiproliferative Effects ◽  
Hela Cell Lines ◽  
Ic50 Value

Medicinal plants maintain the health and vitality of individuals, and also have potential curative effect on various diseases, including cancer. In this study were investigated the antiproliferative effects of water extracts of previously obtained ethanolic dry extracts of three different medicinal plants (Echinacea angustifolia, Salvia officinalis and Melissa officinalis) on cell lines derived from human cervix adenocarcinoma (HeLa cells). The best cytotoxic activity (IC50 = 43.52 ?g/ml) on HeLa cell lines was exhibited by Echinacea angustifolia. The extract of Salvia officinalis also showed a good cytotoxic activity against HeLa cell lines; the IC50 value was 70.41 ?g/ml. Melissa officinalis manifested a slightly weaker cytotoxic activity and an IC50 value of 122.22 ?g/ml.

Interaction of Candida albicans with genital mucosa: effect of sex hormones on adherence of yeasts in vitro

1988 ◽  
Vol 34 (3) ◽  
pp. 224-228 ◽  
Author(s):  
Aliza Kalo ◽  
Esther Segal
Keyword(s):  
Candida Albicans ◽  
Hela Cell ◽  
Sex Hormones ◽  
Hela Cells ◽  
Cell Types ◽  
Vaginal Candidiasis ◽  
Genital Mucosa ◽  
Hela Cell Lines ◽  
I Cells

Findings from our previous studies revealed a correlation between the level of adherence in vitro of Candida albicans to human exfoliated vaginal epithelial cells (VEC) and the hormonal status of the cell donors. In the present study we investigated the effect of the sex hormones estradiol, estriol, progesterone, and testosterone on the binding of the yeasts to HeLa cell lines and VEC in vitro. Monolayers of HeLa cells were exposed to the hormones and yeasts under controlled conditions. The number of adherent yeasts per square millimetre of HeLa cell monolayers and the percentage of VEC with adherent yeasts was estimated by microscopic counts. The results showed that the tested sex hormones affected at various degrees the adhesion of yeasts to HeLa cells or VEC. Progesterone had the most marked effect, leading to a significant increase in the number of adherent yeasts to HeLa cells or in the percentage of adhesion of VEC. In addition, VEC were separated on Percoll gradients into the two cell types: superficial (S) and intermediate (I), cell types which appear physiologically under increased serum levels of estradiol or progesterone, respectively. Adhesion assays with the separated cell populations revealed an increased binding capacity of the I cells. The finding that progesterone increased the adherence of yeasts to genital mucosa and that VEC of the I type have a higher capacity to adhere the yeasts is compatible with our previous observation that increased numbers of I cells, appearing under high level of progesterone, are found in situations known to have predisposition to vaginal candidiasis. Thus, our data point to a possible involvement of the hormone progesterone in the adherence of C. albicans to genital epithelium.

scholarly journals The potency of chitosan-based Pinus merkusii bark extract nanoparticles as anti-cancer on HeLa cell lines

2019 ◽  
Vol 12 (10) ◽  
pp. 1616-1623
Author(s):  
Annise Proboningrat ◽  
Amaq Fadholly ◽  
Regina Purnama Dewi Iskandar ◽  
Agung Budianto Achmad ◽  
Fedik Abdul Rantam ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Hela Cell ◽  
Hela Cells ◽  
Therapeutic Potential ◽  
Bark Extract ◽  
Cytotoxicity Test ◽  
Caspase 9 ◽  
Hela Cell Lines ◽  
Pinus Merkusii

Background and Aim: Cervical cancer accounts for the fourth as a cause of death from cancer in women worldwide, with more than 85% of events and deaths occurring in developing countries. The main problems of chemotherapy are the lack of selectivity and drug resistance. This study aimed to investigate the signal transduction of chitosan-based Pinus merkusii bark extract nanoparticles (Nano-PMBE) as an anticancer on HeLa cell line. Materials and Methods: Nano-PMBE was prepared based on the ionic gelation method. Its anticancer activities in HeLa cells were investigated through cytotoxicity test, cell cycle, and apoptosis analysis. The expression of p53 and caspase-9 was also observed. Results: The results showed that Nano-PMBE has a size of 394.3 nm. Meanwhile, the Nano-PMBE was cytotoxic to HeLa cells ( IC50 of 384.10 μg/ml), caused G0/G1 phase arrest and cell apoptosis in HeLa cells. Besides, the expression of p53 and caspase-9 has increased. Conclusion: The results showed a notable anticancer effect of Nano-PMBE by arresting the cell cycle and inducing apoptosis in HeLa cells, suggesting that it might have therapeutic potential for cervical cancer. Further research is needed to find out more about the anticancer mechanism of Nano-PMBE in HeLa cells to in vivo and clinical studies.

scholarly journals Identification of Spiro-Fused Pyrrolo[3,4-a]pyrrolizines and Tryptanthrines as Potential Antitumor Agents: Synthesis and In Vitro Evaluation

2021 ◽  
Vol 22 (21) ◽  
pp. 11997
Author(s):  
Diana. K. Latypova ◽  
Stanislav V. Shmakov ◽  
Sofya A. Pechkovskaya ◽  
Alexander S. Filatov ◽  
Alexander V. Stepakov ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Cervical Carcinoma ◽  
Hela Cells ◽  
Antitumor Agents ◽  
Hela Cell Lines ◽  
M Phase ◽  
Induced Apoptosis ◽  
Potential Antitumor

A series of heterocyclic compounds containing a spiro-fused pyrrolo[3,4-a]pyrrolizine and tryptanthrin framework have been synthesized and studied as potential antitumor agents. Cytotoxicity of products was screened against human erythroleukemia (K562) and human cervical carcinoma (HeLa) cell lines. Among the screened compounds. 4a, 4b and 5a were active against human erythroleukemia (K562) cell line, while 4a and 5a were active against cervical carcinoma (HeLa) cell line. In agreement with the DNA cytometry studies, the tested compounds have achieved significant cell-cycle perturbation with higher accumulation of cells in G2/M phase and induced apoptosis. Using confocal microscopy, we found that with 4a and 5a treatment of HeLa cells, actin filaments disappeared, and granular actin was distributed diffusely in the cytoplasm in 76–91% of cells. We discovered that HeLa cells after treatment with compounds 4a and 5a significantly reduced the number of cells with filopodium-like membrane protrusions (from 63 % in control cells to 29% after treatment) and a decrease in cell motility.

scholarly journals TOTAL PHENOLIC CONTENTS, ANTIOXIDANT, ANTICANCER AND ANTIDIABETIC PROPERTIES OF Myrmecodia tuberosa (RUBIACEAE)

2013 ◽  
Vol 9 (3) ◽  
pp. 2000-2004
Author(s):  
Saidi Rasemi ◽  
Dr Heng Yen Khong ◽  
Rohaya Ahmad ◽  
Mizaton Hazizul Hasan
Keyword(s):  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Total Phenolic ◽  
Cytotoxicity Test ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Hela Cell Lines ◽  
Ic50 Value ◽  
Cancer Agent

Myrmecodia tuberosa, locally known as “Sarang Semut”, belongs to the Rubiaceae family. The aim of the study is to investigate the total phenolic contents and bioactivity of the crude extract of M. tuberosa. The tuber, bark and leaves of the plant were cut, washed and air-dried. The plant was extracted with ethyl acetate and ethanol to yield EtOAc and EtOH crudes. The crudes were then tested for antioxidant 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) assay, α-glucosidase assay for anti-diabetic activity and [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium] MTT Assay for cytotoxicity test. The results showed that bark ethanolic extract demonstrated the highest DPPH scavenging of 95.16% while other crudes were ranging from 92.77% to 94.55% scavenging of DPPH compared to ascorbic acid. The tuber ethyl acetate extract demonstrated the highest inhibition of α -glucosidase enzyme with 72.58% while other crudes were ranging from 63.27% to 72.46% inhibition. In addition, the ethanolic tuber extract exhibited the strongest cytotoxicity against Human colorectal Cancer (HT-29) and Cervical Cancer (HeLa) cell lines with the IC50 value of 16 µg/mL and 14 µg/mL respectively. Meanwhile, the ethanolic bark extract exhibited the strongest cytotoxicity against Human Breast Cancer (MCF-7) with IC50 value of 6.0µg/mL. Furthurmore, the tuber extracts contained the highest phenolic content with 1087mg GAE/ g extract compared to the leaves and barks of the plants. The findings suggest that the whole part of M. tuberosa is a potential natural source for anti-oxidative, anti-diabetic and anti-cancer agent.

scholarly journals Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells

2017 ◽  
Vol 7 (2) ◽  
pp. 60
Author(s):  
Erlina Rivanti ◽  
Bani Adlina Shabrina ◽  
Ika Nurzijah ◽  
Cyndwika Ayu ◽  
Adam Hermawan
Keyword(s):  
Cancer Cells ◽  
Hela Cells ◽  
Breast Cancer Cells ◽  
Ethanolic Extract ◽  
Vero Cells ◽  
Cytotoxic Activities ◽  
Colon Cancer Cells ◽  
Caesalpinia Sappan ◽  
Ic50 Value ◽  
Cervical Cancer Cells

The present study investigate the selectivity of heartwood of secang ethanolic extract (SEE) on T47D breast cancer cells, WiDr colon cancer cells, and HeLa cervical cancer cells, compared to Vero normal epithelial cells. The cytotoxic effect was evaluated by using MTT assay  with 24-hour treatment to get IC50values. Selectivity was evaluated by using selectivity index (SI). SEE had a potent cytotoxic activity on T47D and WiDr cancer cells (IC50 <100 µg/ml). IC50 value of HeLa cancer cells was observed on moderate cytotoxic (100 <IC50 <1000 µg/ml). SEE demonstrated more selective to T47D and WiDr than Vero cells (SI > 3), while in HeLa cells is not selective (SI < 3). This result indicating its potential of Caesalpinia sappan as a chemopreventive agent in cancer therapy.Keywords: Cancer, selectivity, Secang, T47D, WiDr, HeLa, Vero

scholarly journals Hesperidin Increase Cytotoxic Activity of Doxorubicin on Hela Cell Line Through Cell Cycle Modulation and Apoptotis Induction

2011 ◽  
Vol 2 (2) ◽  
pp. 267 ◽  
Author(s):  
Indri Kusharyanti ◽  
Larasati Larasati ◽  
Ratna Asmah Susidarti ◽  
Edy Meiyanto
Keyword(s):  
Cell Cycle ◽  
Hela Cell ◽  
Hela Cells ◽  
Chemotherapeutic Agent ◽  
Inhibitory Effect ◽  
Adverse Side Effect ◽  
Cytotoxic Activities ◽  
Hela Cell Lines ◽  
Cell Cycle Modulation ◽  
Apoptotic Effect

Combination of chemotherapeutic agent and chemopreventive agent is being a new approach in cancer treatment. This is aimed at enhancing the effectivity and also reducing drug resistance and adverse side effect of the chemotherapeutic agent. Hesperidin, a citrus flavonoid has reported to reduce the proliferation of many cancer cells. The objectives of this study were to investigate cytotoxic activities, cell cycle modulation and apoptosis induction of hesperidin and its combination with doxorubicin on Hela cell lines. MTT [3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide] assay was used to measure the growth inhibitory effect of hesperidin and its combination with doxorubicin on Hela cells. Cell cycle profile was determined by flowcytometry and the data obtained was analyzed by using ModFit LT 3.0 program. Apoptosis assay was done using double staining method using ethidium-bromide and acridine-orange. Hesperidin inhibited cell growth with IC50 48 μM, while the IC50 of doxorubicin was 1000 nM. Combination of 500 nM doxorubicin and 6 μM hesperidin showed strongest inhibitory effect toward Hela cells. Hesperidin of 24 µM accumulated HeLa cells at G1 phase, but its combination with 500 nM Doxorubicin gave G1 and S phase accumulation at 24 h incubation. Both of Hesperidin and Doxorubicin were capable of inducing apoptosis. In accordance of the apoptotic effect, hesperidin, doxorubicin and their combination decreased the expression Bcl-2 and increased the expression of Bax. According to this result, hesperidin has a potency to be developed as co-chemotherapeutic agent for cervical cancer.Keywords: Cochemotherapy, Hesperidin, Doxorubicin, Hela, MTT assay

scholarly journals Antiproliferative effects of Vanilla planifolia leaf extract against breast cancer MCF-7 cells

2018 ◽  
Vol 8 (1) ◽  
pp. 51
Author(s):  
Vijaybabu Kaliappan ◽  
Punnagai Kumaravelu
Keyword(s):  
Breast Cancer ◽  
Dna Fragmentation ◽  
Mtt Assay ◽  
Leaf Extract ◽  
Critical Research ◽  
Cancer Disease ◽  
Antiproliferative Effects ◽  
Ic50 Value ◽  
Study Results ◽  
Mcf 7

Background: Breast cancer is the most common cancer disease among females in India and worldwide. This needs a critical research for finding the drugs to treat breast cancer with less side effects. The aim of the present study is to reveal the anti-proliferative effects of vanilla extract against MCF-7 cells.Methods: To reveal anti proliferative effects of vanilla leaf extract, MTT assay, cell cycle analysis and DNA fragmentation assay was performed as per standard protocols.Results: MTT assay showed decrease in cell viability with increase of dose of extract and revealed IC50 value at 31.2µg/ml. DNA fragmentation was seen in extract treated cells.Conclusions: The results of the present study confirm the antiproliferative property of vanilla leaf extract in MCF-7 cells. This study results conclude vanilla leaf extract as an effective plant source medicament for treating breast cancer.

scholarly journals Isolation and Characterization of Hermetia Illucens Larval Protein for the Assessment of Inhibitory Activity against MCF7 and HeLa cell Lines

2020 ◽  
Vol 9 (3) ◽  
pp. 3045-3050
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Hela Cells ◽  
Inhibitory Activity ◽  
Crude Protein ◽  
The Body ◽  
Assay Method ◽  
Hermetia Illucens ◽  
Isolation And Characterization ◽  
Hela Cell Lines

Cancer has a major impact on society having the highest morbidity and mortality rate. Approximately 70% of deaths are predominantly caused due to cancer and it prevails in low and middle-income countries, as they are unaware of the signs and symptoms, treatment methods, non-availability of cancer drugs and the incurrence of huge economic burden on the family. As per the literature, the chemotherapeutic drug has numerous side effects and occasionally it may lead to death. Based on these inferences, there is a requirement to identify therapeutic peptides that are more effective for cancer treatment with less toxic effects on the body. Peptides have a great advantage to fight against the disease efficiently. Several flies have anticancer, antibacterial, antifungal and antioxidant properties that help to control disease and inhibit cancer progression. In the present research, we have utilized Hermetia illucens has great anticancer peptides that can control cancer growth. In the current research, we utilized crude protein extraction from Hermetia illucens larvae, to discover the inhibitory activity against MCF7 and HeLa cell lines. by adopting the MTT assay method, along with reference drug Camptothecin. Bradford assay results helped to identify that 23.5µg/ml concentration of protein is present in the crude sample. Flow cytometry was used to calculate the % of cell violations and IC50 values were observed in HeLa (19.88±50.3μg/mL) and MCF7 (224.68±50.3μg/mL) cells. Cell cycle inhibition was observed in HeLa cells of G1 (57.3±1.2) and, S (19.8±1.3) phase of dose-dependent with the significance of (P<0.05). Hermetia illucens crude protein has significant inhibition with HeLa cells that have the best anticancer proteins in human cervical carcinoma cells.

The Cytotoxicity of Ethanolic Extract of Allium cepa L. on Hela Cell Lines

2021 ◽  
pp. 4969-4972
Author(s):  
Annise Proboningrat ◽  
Shara Jayanti ◽  
Amaq Fadholly ◽  
Arif N. M. Ansori ◽  
Naimah Putri ◽  
...  
Keyword(s):  
Side Effects ◽  
Allium Cepa ◽  
Hela Cells ◽  
Cytotoxic Effect ◽  
Test Procedure ◽  
Ethanolic Extract ◽  
Cytotoxicity Test ◽  
Drug Candidates ◽  
Hela Cell Lines ◽  
Allium Cepa L

Cancer is a significant global health problem today and responsible for most deaths after cardiovascular diseases. One of the conventional modalities for cancer treatment is chemotherapy. However, it has many side effects and complications. Therefore, the search for novel drug candidates from natural ingredients that are more effective and have minimal side effects is critical. This study is aimed to determine the cytotoxic effect of Allium cepa L. extract on human cervical cancer cells. The cytotoxicity test procedure was performed using MTT assay against HeLa cells. The result revealed that Allium cepa L. ethanolic extract has a weak cytotoxic effect on HeLa cells with IC50 of 900.88 µg/ml. Further research into Allium cepa L. is required to isolate and identify its components and determine their potential as new agents against HeLa cells.

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