scholarly journals Staphylococcus aureus Manganese Transport Protein C Is a Highly Conserved Cell Surface Protein That Elicits Protective Immunity Against S. aureus and Staphylococcus epidermidis

2012 ◽  
Vol 205 (11) ◽  
pp. 1688-1696 ◽  
Author(s):  
Annaliesa S. Anderson ◽  
Ingrid L. Scully ◽  
Yekaterina Timofeyeva ◽  
Ellen Murphy ◽  
Lisa K. McNeil ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Surface ◽  
Staphylococcus Epidermidis ◽  
Protective Immunity ◽  
Protein C ◽  
Surface Protein ◽  
Transport Protein ◽  
Cell Surface Protein ◽  
Manganese Transport

scholarly journals Immunisation With Immunodominant Linear B Cell Epitopes Vaccine of Manganese Transport Protein C Confers Protection against Staphylococcus aureus Infection

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0149638 ◽  
Author(s):  
Hui-Jie Yang ◽  
Jin-Yong Zhang ◽  
Chao Wei ◽  
Liu-Yang Yang ◽  
Qian-Fei Zuo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
B Cell ◽  
Protein C ◽  
Transport Protein ◽  
B Cell Epitopes ◽  
Aureus Infection ◽  
Manganese Transport ◽  
Linear B

scholarly journals Protein rib: a novel group B streptococcal cell surface protein that confers protective immunity and is expressed by most strains causing invasive infections.

1993 ◽  
Vol 177 (6) ◽  
pp. 1593-1603 ◽  
Author(s):  
M Stålhammar-Carlemalm ◽  
L Stenberg ◽  
G Lindahl
Keyword(s):  
Cell Surface ◽  
Protective Immunity ◽  
Group B Streptococcus ◽  
Rabbit Antiserum ◽  
Surface Protein ◽  
Amino Acid Sequences ◽  
Cell Surface Protein ◽  
Type Iii ◽  
Invasive Infections ◽  
Group B

The group B Streptococcus, an important cause of invasive infections in the neonate, is classified into four major serotypes (Ia, Ib, II, and III) based on the structure of the polysaccharide capsule. Since the capsule is a known virulence factor, it has been extensively studied, in particular in type III strains, which cause the majority of invasive infections. Two cell surface proteins, alpha and beta, have also been studied in detail since they confer protective immunity, but these proteins are usually not expressed by type III strains. We describe here a cell surface protein, designated protein Rib (resistance to proteases, immunity, group B), that confers protective immunity and is expressed by most strains of type III. Protein Rib was first identified as a distinct 95-kD protein in extracts of a type III strain, and was purified to homogeneity from that strain. Rabbit antiserum to protein Rib was used to demonstrate that it is expressed on the cell surface of 31 out of 33 type III strains, but only on 1 out of 25 strains representing the other three serotypes. Mouse protection tests showed that antiserum to protein Rib protects against lethal infection with three different strains expressing this antigen, including a strain representing a recently identified high virulence type III clone. Protein Rib is immunologically unrelated to the alpha and beta proteins, but shares several features with the alpha protein. Most importantly, the NH2-terminal amino acid sequences of the Rib and alpha proteins are identical at 6 out of 12 positions. In addition, both protein Rib and the alpha protein are relatively resistant to trypsin (and Rib is also resistant to pepsin) and both proteins vary greatly in size between different clinical isolates. Finally, both protein Rib and the alpha protein exhibit a regular ladderlike pattern in immunoblotting experiments, which may reflect a repetitive structure. Taken together, these data suggest that the Rib and alpha proteins are members of a family of proteins with related structure and function. Since protein Rib confers protective immunity, it may be valuable for the development of a protein vaccine against the group B Streptococcus, an encapsulated bacterium.

Elevated sEPCR and PAI-1 Levels, but Not sThrombomodulin Levels in Normal Pregnancy.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3996-3996
Author(s):  
Yale S. Arkel ◽  
Michael J. Paidas ◽  
De-Hui W. Ku ◽  
Elizabeth Triche ◽  
Xuam Lam ◽  
...  
Keyword(s):  
Cell Surface ◽  
Inflammatory Cytokines ◽  
Protein C ◽  
Surface Protein ◽  
Normal Pregnancy ◽  
Cell Surface Protein ◽  
A Cell ◽  
Pai 1 ◽  
In Pregnancy ◽  
Pro Inflammatory Cytokines

Abstract Pregnancy is a prothrombotic state. The protein C system is felt to be the major regulatory mechanism for the control of thrombin formation in pregnancy. The protein C (PC) system consists of PC, protein S (PS), thrombomodulin (TM) and endothelial protein C receptor (EPCR). The other component for the control of thrombosis is the fibrinolytic system. Plasminogen activator inhibitor-1(PAI-1) is a major down-regulator of fibrinolysis by its effect on plasmin formation and has been shown to be increased in pregnancy. In this study we examined the levels of plasma EPCR (sEPCR), TM (sTM) and PAI-1antigen in normal pregnancies (NP). A total of 82 1st trimester, 64 2nd trimester, and 78 3rd trimester samples from NP patients were used for this study. TM a cell surface protein, found primarily on endothelial cells (ECs) but also identified on trophoblasts and endometrial decidual cells (DCs) of pregnancy, is known to shed off ECs due to metalloproteinases stimulated by thrombin and pro-inflammatory cytokines or in the setting of EC damage. There are reports of increased adverse pregnancy outcome (APO) associated with high levels of plasma TM. Although the sTM level in NP is greater than non-pregnant controls contrary to other reports we did not find a statistically significant increase in TM with gestation. EPCR is a cell surface protein and is primarily on ECs and we have identified it on DCs. Like TM it is shred from ECs by metalloproteinases due to thrombin and pro-inflammatory cytokines. Cell surface TM and EPCR are central to the formation of activated protein C (APC). Our data would indicate that sEPCR statistically increased with gestation(1st trimester compared to 2nd [doubles] and 3rd [1.5 fold]. sEPCR has the property of binding free APC and decreasing its availability as an anticoagulant to degrade FVIIIa and FVa. Therefore during pregnancy the PC system is impaired by the known decrease in PS, acquired resistance to APC and the increased levels of sEPCR which would further inhibit the actions of APC. Finally we have confirmed that PAI-1 does increase in normal gestation with statistically significant rises in the PAI-1 antigen. To date, measurement of PAI-1 levels has had limited predictive value. Exploration of the relationship between PAI-1 levels, 4G/4G polymorphism and possibly PAI-1 antibodies may provide better APO risk assessment. Overall our data indicates that in normal pregnancy the prothrombotic milieu is increased by the impaired PC system by the increased level of sEPCR and the fibrinolytic system is decreased by the rise of PAI-1. The sTM although greater than non-pregnant controls does not seem to increase with NP gestation. This would suggest that serial measurements of sTM in possible problematic pregnancies may provide additional information particularly if the non-pregnant baseline value is known. This will require prospective controlled studies of larger NP and APO patients. The assessment of decreased APC plasma function may also provide added information on the risk factors for APO. This may be related in some part to the level of sEPCR. Table 1 1st trimester 2nd trimester 3rd trimester 1st vs 2nd 1st vs 3rd 2nd vs 3rd NS=non significant sEPCR (ng/ml) 44.9+/−23 94.9+/−62 62.9+/−27.3 p<0.05 p<0.05 p<0.05 sTM (ng/ml) 35.2+/−21.4 33.5+/−10.6 35.3+/−33.6 NS NS NS PAI-1 (ng/ml) 19.3+/−12.9 48.4+/−23.2 72.4+/−27.3 p<0.05 p<0.05 p<0.05

scholarly journals Staphylococcus aureus expresses a cell surface protein that binds both IgG and β2-glycoprotein I

Microbiology ◽  
1999 ◽  
Vol 145 (1) ◽  
pp. 177-183 ◽  
Author(s):  
Lihong Zhang ◽  
Karin Jacobsson ◽  
Katrin Ström ◽  
Martin Lindberg ◽  
Lars Frykberg
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Surface ◽  
Surface Protein ◽  
Cell Surface Protein ◽  
Glycoprotein I ◽  
A Cell

scholarly journals Staphylococcus aureus Manganese Transport Protein C (MntC) Is an Extracellular Matrix- and Plasminogen-Binding Protein

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e112730 ◽  
Author(s):  
Natália Salazar ◽  
Mónica Marcela Castiblanco-Valencia ◽  
Ludmila Bezerra da Silva ◽  
Íris Arantes de Castro ◽  
Denize Monaris ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Extracellular Matrix ◽  
Protein C ◽  
Binding Protein ◽  
Transport Protein ◽  
Manganese Transport

Immunogold and immunoblot studies on a cell surface protein found in primary mesenchyme cells and in the cortical secretory vesicles of the sea urchin egg

1987 ◽  
Vol 45 ◽  
pp. 832-833
Author(s):  
G.L. Decker ◽  
M.C. Valdizan
Keyword(s):  
Cell Surface ◽  
Sea Urchin ◽  
Surface Protein ◽  
Egg Cell ◽  
Secretory Vesicles ◽  
Cell Surface Protein ◽  
Surface Complexes ◽  
Mesenchyme Cells ◽  
Lowicryl K4m ◽  
Primary Mesenchyme Cells

A monoclonal antibody designated MAb 1223 has been used to show that primary mesenchyme cells of the sea urchin embryo express a 130-kDa cell surface protein that may be directly involved in Ca2+ uptake required for growth of skeletal spicules. Other studies from this laboratory have shown that the 1223 antigen, although in relatively low abundance, is also expressed on the cell surfaces of unfertilized eggs and on the majority of blastomeres formed prior to differentiation of the primary mesenchyme cells.We have studied the distribution of 1223 antigen in S. purpuratus eggs and embryos and in isolated egg cell surface complexes that contain the cortical secretory vesicles. Specimens were fixed in 1.0% paraformaldehyde and 1.0% glutaraldehyde and embedded in Lowicryl K4M as previously reported. Colloidal gold (8nm diameter) was prepared by the method of Mulpfordt.

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