Secretor and Salivary ABO Blood Group Antigen Status Predict Rotavirus Vaccine Take in Infants

Abstract Background Histo–blood group antigen (HBGA) Lewis/secretor phenotypes predict genotype-specific susceptibility to rotavirus gastroenteritis (RVGE). We tested the hypothesis that nonsecretor/Lewis-negative phenotype leads to reduced vaccine take and lower clinical protection following vaccination with G1P[8] rotavirus vaccine (RV1) in Malawian infants Methods A cohort study recruited infants receiving RV1 at age 6 and 10 weeks. HBGA phenotype was determined by salivary enzyme-linked immunosorbent assay (ELISA). RV1 vaccine virus shedding was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in stool collected on alternate days for 10 days post-immunization. Plasma rotavirus–specific immunoglobulin A was determined by ELISA pre- and post-immunization. In a case-control study, HBGA phenotype distribution was compared between RV1-vaccinated infants with RVGE and 1:1 age-matched community controls. Rotavirus genotype was determined by RT-PCR. Results In 202 cohort participants, neither overall vaccine virus fecal shedding nor seroconversion differed by HBGA phenotype. In 238 case-control infants, nonsecretor phenotype was less common in infants with clinical vaccine failure (odds ratio [OR], 0.39; 95% confidence interval [CI], 0.20–0.75). Nonsecretor phenotype was less common in infants with P[8] RVGE (OR, 0.12; 95% CI, 0.03–0.50) and P[4] RVGE (OR, 0.17; 95% CI, 0.04–0.75). Lewis-negative phenotype was more common in infants with P[6] RVGE (OR, 3.2; 95% CI, 1.4–7.2). Conclusions Nonsecretor phenotype was associated with reduced risk of rotavirus vaccine failure. There was no significant association between HBGA phenotype and vaccine take. These data refute the hypothesis that high prevalence of nonsecretor/Lewis-negative phenotypes contributes to lower rotavirus vaccine effectiveness in Malawi.

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Ecological studies of intestinal bacteria. Relation between the specificity of fecal ABO blood group antigen-degrading enzymes from enteric bacteria and the ABO blood group of the human host

Journal of Clinical Investigation ◽

10.1172/jci106024 ◽

1969 ◽

Vol 48 (4) ◽

pp. 664-673 ◽

Cited By ~ 19

Author(s):

Lansing C. Hoskins

Keyword(s):

Blood Group ◽

Intestinal Bacteria ◽

Enteric Bacteria ◽

Blood Group Antigen ◽

Abo Blood Group ◽

Ecological Studies ◽

Human Host ◽

Degrading Enzymes

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Prevalence of Weak D (Du) in Blood Donors in a Referral Teaching Hospital

International Journal of Medical and Dental Sciences ◽

10.18311/ijmds/2017/18841 ◽

2017 ◽

Vol 6 (2) ◽

pp. 1509

Author(s):

H. S. Lamba ◽

Kulbir Kaur ◽

Kulwant Kaur ◽

Amarjit Singh Vij

Keyword(s):

Patient Care ◽

Blood Group ◽

Teaching Hospital ◽

Blood Donors ◽

Blood Product ◽

Blood Group Antigen ◽

Abo Blood Group ◽

Blood Samples ◽

Donor Population ◽

Gel Technique

Background: Rh D is the most important Blood Group antigen after ABO Blood group antigen for transfusion purpose. All negative blood units by routine methods must be tested to detect weak D using IAT method. When the test for D and Du is positive, the label should read Rh(D) Positive . When the test for D and Du is negative, the label should read Rh(D) Negative.Objective: To know the prevalence of weak D in the donor population. No study has been done in this part of the country earlier. It will help in the knowledge of weak D, which is very important for better patient care and prevent allo-immunzation in blood recipients.Materials and Methods: Blood samples were tested by ID Gel technique or by tube method with two anti D reagents - anti-D IgM monoclonal and blend of anti-D IgM&amp;IgG. All negative samples were further tested for weak D in IAT phase by LISS/Coombs' gel card.Results: A total of 13043 samples were tested from January 2011 to December 2013. 12196 were Rh positive and 847 were Rh D negative. Weak D was positive in 8 samples.Conclusion: The study shows the prevalence of weak D as 0.07% in blood donors who were primarily from in and around Jalandhar in Punjab. These donors may have posed problem to the recipients of blood and blood product and their detection prevented them from alloimunisation.

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A phenome-wide association study of ABO blood groups

BMC Medicine ◽

10.1186/s12916-020-01795-4 ◽

2020 ◽

Vol 18 (1) ◽

Author(s):

Shun Li ◽

C. M. Schooling

Keyword(s):

Blood Pressure ◽

Body Composition ◽

Association Study ◽

Blood Group ◽

Density Lipoprotein ◽

Blood Group Antigen ◽

Abo Blood Group ◽

Blood Group Antigens ◽

Nucleotide Polymorphisms ◽

Tag Snp

Abstract Background ABO blood group is associated with differences in lifespan, cardiovascular disease, and some cancers, for reasons which are incompletely understood. To gain sex-specific additional insight about potential mechanisms driving these common conditions for future interventions, we characterized associations of ABO blood group antigen across the phenotype sex-specifically. Methods We performed a phenome-wide association study (PheWAS) assessing the association of tag single nucleotide polymorphisms (SNPs) for ABO blood group antigens (O, B, A1, and A2) with 3873 phenotypes. Results The tag SNP for the O antigen was inversely associated with diseases of the circulatory system (particularly deep vein thrombosis (DVT)), total cholesterol, low-density lipoprotein cholesterol (LDL-C), and ovarian cancer, and positively associated with erythrocyte traits, leukocyte counts, diastolic blood pressure (DBP), and healthy body composition; the tag SNP for the A1 antigen tended to have associations in reverse to O. Stronger associations were more apparent for men than women for DVT, DBP, leukocyte traits, and some body composition traits, whereas larger effect sizes were found for women than men for some erythrocyte and lipid traits. Conclusion Blood group has a complex association with cardiovascular diseases and its major risk factors, including blood pressure and lipids, as well as with blood cell traits and body composition, with some differences by sex. Lower LDL-C may underlie some of the benefits of blood group O, but the complexity of associations with blood group antigen suggests overlooked drivers of common chronic diseases.

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