Comparative Studies of Thin Layer Chromatographic Systems for the Separation and Identification of Photoalteration Products of Parathion

1973 ◽  
Vol 56 (2) ◽  
pp. 338-340
Author(s):  
Ronald L Joiner ◽  
Karl P Baetcke
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Petroleum Ether ◽  
Infrared Spectra ◽  
Ethyl Ether ◽  
Comparative Studies ◽  
Glacial Acetic Acid ◽  
Ammonium Hydroxide ◽  
Rf Values

Abstract Twelve thin layer chromatographic systems were compared for the separation of photoalteration products of parathion. Rf values for each component in each system are presented. Infrared spectra confirmed the identity of separated components. The following 4 systems were designated superior and gave excellent separation of the components: petroleum ether-ethyl ether-glacial acetic acid (80+15+5 and 50+45+5), methanol-chloroform-ammonium hydroxide (24+75+3.5), and methanol-chloroform-petroleum ether (10+20+70).

European Taxa of Saxicolous Lecidella Containing Chloroxanthones:Identification of Patterns Using Thin Layer Chromatography

1992 ◽  
Vol 24 (4) ◽  
pp. 383-397 ◽  
Author(s):  
Ch. Leuckert ◽  
J.-G. Knoph
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Glacial Acetic Acid ◽  
Uv Light ◽  
Rf Values ◽  
Layer Chromatography ◽  
Spray Reagents

AbstractA method using TLC to recognize compound patterns consisting almost entirely of chloroxanthones in European saxicolous species of Lecidella is described. These are necessary for diagnosis of the taxa studied and of their chemotypes. Two solvents with complementary separatingqualities were required: C, toluene-glacial acetic acid (100:15); J, dichloromethane-acetone (4:1). Although the Rf values in solvent J strongly depend on the concentration of the substances, it is very suitable for identification because the xanthones in this system reveal distinct and characteristic fluorescence colours in UV light, simplifying interpretation enormously. No spray reagents are used. Identifications are made by co-chromatography with authentic lichencompounds

scholarly journals Thin layer chromatographic compatibility study in preformulation of new transdermal therapeutic systems

2020 ◽  
Vol 66 (1) ◽  
pp. 23-29
Author(s):  
Octavia-Laura Moldovan ◽  
Diana-Nicoleta Brihan ◽  
Nicoleta Todoran ◽  
Aura Rusu
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Mobile Phase ◽  
Glacial Acetic Acid ◽  
Methylene Chloride ◽  
Hydroxyethyl Cellulose ◽  
Compatibility Study ◽  
Chemical Interactions ◽  
Miconazole Nitrate ◽  
Active Substances

AbstractObjective: The compatibility of four binary active substances combinations adapalene – levofloxacin (ADP-LFX), adapalene – miconazole nitrate (ADP-MCZ), levofloxacin – meloxicam (LFX-MLX) and levofloxacin – miconazole nitrate (LFX-MCZ) was analysed to be comprised in new transdermal therapeutic systems. Also, the compatibility of selected active substances and four polymeric excipients (hydroxypropyl methyl-cellulose - HPMC 15000, hydroxypropyl methylcellulose - HPMC E5, ethyl cellulose - EC 10, and hydroxyethyl cellulose – HEC) was studied.Methods: Thin layer chromatographic method (TLC) and four selected mobile phases were used. On the plate (in situ) were obtained the binary combinations (active substances and active substance-polymer).Results: A good compatibility of ADP-LFX was found using ammonia : methanol : acetonitrile : methylene chloride 2:4:1:4 mobile phase. Using chloroform : acetone : glacial acetic acid 34:4:3 on the chromatogram of ADP-MCZ, only ADP spots appeared but without changes in the shape of the spots and Rf values. Any modifications of LFX and MLX spots (from LFX-MLX mixture) had been observed using toluene : glacial acetic acid : methanol 11:1:0.5 mobile phase, although LFX spots have remained on the baseline. Only LFX spots were visible from LFX-MLX and LFX-MCZ mixtures (ammonia : methanol : acetonitrile : methylene chloride 2:4:1:4 mobile phase). Distinctive spots were observed for ADP, LFX and MLX with variable results from no chemical interactions to limited chemical interactions when the compatibility with polymers was verified.Conclusions: ADP-LFX and LFX-MLX mixtures were found to be compatible. ADP with HPMC polymers and LFX with HPMC E5 and HEC had presented excellent compatibility; for the other binary combinations, different analytical methods will be necessary.

HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS QUANTITATION OF LOPINAVIR AND RITONAVIR IN TABLET FORMULATION

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (01) ◽  
pp. 23-29
Author(s):  
J. D. Fegade ◽  
◽  
N. D Chawla ◽  
R. Y. Chaudhari ◽  
V. R. Patil
Keyword(s):  
Acetic Acid ◽  
High Performance ◽  
Chromatographic Method ◽  
Glacial Acetic Acid ◽  
Solvent System ◽  
Tablet Formulation ◽  
Ich Guidelines ◽  
Rf Values ◽  
Aluminum Plates ◽  
Hptlc Method

The present work describes a simple, accurate and precise HPTLC method for simultaneous quantitation of ritonavir (RVR) and lopinavir (LVR) in tablet formulation. Chromatographic separation of both drugs was performed on precoated aluminum plates, silica gel 60 F254 as the stationary phase and the solvent system consisted of toluene: ethyl acetate: methanol: glacial acetic acid in the ratio of 6.5:2.5:0.5:0.5(v/v/v/v). Densitometric evaluation of the separated zones was performed at 266 nm. The two drugs were satisfactorily resolved with Rf values of 0.242 0.03 and 0.413 0.02 for RVR and LVR, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (400-2000 ng/spot for RVR and 1600-8000 ng/spot for LVR), precision (intra-day RSD 0.16-0.38% and inter-day RSD 0.21-0.60 % for RVR and intra-day RSD 0.35-0.58 % and inter-day RSD 0.26-0.55 % for LVR) and recovery (99.54 0.62 % for RVR and 100.45 0.65 % for LVR), in accordance with ICH guidelines.

scholarly journals A Rapid Thin Layer Chromatographic Method for Simultaneous Screening of Albendazole and Ivermectin in Formulations Prescribed for Human or Veterinary Use

2019 ◽  
Vol 31 (4) ◽  
pp. 896-900
Author(s):  
R.P. Pawar ◽  
P. Mishra ◽  
A. Durgbanshi ◽  
D. Bose
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Pharmaceutical Preparations ◽  
Cost Effective ◽  
Dosage Forms ◽  
Thin Layer Chromatographic Plate ◽  
Rf Values ◽  
Chromatographic Plate

An easy and selective thin layer chromatographic method has been developed and experimentally validated for the simultaneous screening of most commonly used anthelmintic drugs i.e. albendazole and ivermectin. Separation of these compounds was attained on silica gel 60 F254 pre-coated thin layer chromatographic plate using an optimized mobile phase of diisopropyl ether:ethyl acetate:glacial acetic acid in the ratio of 7:3:0.1 (v/v), respectively at pH 3.5. The calculated Rf values for albendazole and ivermectin were 0.65 and 0.38, respectively and the LOD was found to be 25 μg/ml and 30 μg/ml for albendazole and ivermectin, respectively. The developed method is selective, sensitive, robust, cost effective, eco-friendly, rapid as well as easy to perform. The developed method was successfully applied for the analysis of albendazole and ivermectin in pharmaceutical preparations marketed as oral suspensions, powder, tablets and injectable of the single or combined dosage forms for human as well as veterinary use. It could also be applied for the simultaneous analysis of both the compounds in other samples.

Xanthotoxin and Bergapten in Spring Parsley

Weed Science ◽  
1970 ◽  
Vol 18 (4) ◽  
pp. 479-480 ◽  
Author(s):  
M. Coburn Williams
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Infrared Spectra ◽  
Growing Season ◽  
Melting Points ◽  
Plant Samples ◽  
Rf Values ◽  
Layer Chromatography

Two phototoxic compounds, xanthotoxin (8-methoxysporalen) and bergapten (5-methoxypsoralen), were isolated from spring parsley [Cymopterus watsonii(Coult. & Rose) Jones] by thin-layer chromatography, and identified by comparison of Rf values, infrared spectra, and melting points, with pure samples. The concentration of xanthotoxin was two to four times that of bergapten in plant samples collected during the 2-month growing season. Seeds contained xanthotoxin and bergapten in equal concentrations.

scholarly journals Simultaneous Estimation of Four Antitussive Components from Herbal Cough Syrup by HPTLC

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Sharada L. Deore ◽  
Payal S. Jaju ◽  
Bhushan A. Baviskar
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
High Performance ◽  
Glacial Acetic Acid ◽  
Simultaneous Estimation ◽  
Retention Factors ◽  
Ich Guidelines ◽  
Cough Syrup ◽  
Hptlc Method

A new simple, rapid, selective and precise high performance thin layer chromatographic (HPTLC) method has been developed for simultaneous estimation of vasicine, glycyrrhizin, eugenol, and cineole in herbal cough syrup. The retention factors of vasicine, glycyrrhizin, eugenol, and cineole are 0.53, 0.44, 0.75, and 0.77, respectively. Chromatography was performed on 60F254 percolated TLC plate using n-hexane : ethyl acetate : glacial acetic acid (8.5 : 1.0 : 0.5 v/v/v). Methods are validated according to ICH guidelines and can be adopted for the routine analysis of vasicine, glycyrrhizin, eugenol and cineole in herbal cough syrup.

Adjuvant and pH Effects on Sethoxydim and Clethodim Activity on Rhizome Johnsongrass (Sorghum halepense)

1989 ◽  
Vol 3 (4) ◽  
pp. 615-620 ◽  
Author(s):  
David C. Bridges
Keyword(s):  
Acetic Acid ◽  
Calcium Hydroxide ◽  
Glacial Acetic Acid ◽  
Ph Dependence ◽  
Ammonium Hydroxide ◽  
Sorghum Halepense ◽  
Ph Effects ◽  
Ph Range

Rhizome johnsongrass control with sethoxydim and clethodim was evaluated with the addition of three adjuvants: Agrioil3, BAS8153, and Li7003. Johnsongrass control was similar with the three adjuvants when either sethoxydim or clethodim was applied alone, but when bentazon was added, johnsongrass control declined with both sethoxydim and clethodim. Control generally declined less with BAS815 than with Agrioil or Li700, but the addition of BAS815 did not completely reverse the effects of adding bentazon to sethoxydim or clethodim. Johnsongrass control was not affected by adjusting pH when sethoxydim or clethodim was applied with bentazon and Agrioil. Johnsongrass control over a pH range of 3.5 to 6.5 via the addition of glacial acetic acid and calcium hydroxide or ammonium hydroxide indicated a lack of pH dependence for these herbicides. The addition of ammonium hydroxide resulted in slight, but inconsistent increases in johnsongrass control.

scholarly journals PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI LAPIS TIPIS DENSITOMETRI UNTUK PENETAPAN KADAR TEOFILIN DAN EFEDRIN HIDROKLORIDA SECARA SIMULTAN PADA SEDIAAN TABLET

2013 ◽  
Vol 15 (1) ◽  
pp. 15-21
Author(s):  
Lestyo Wulandari ◽  
Yuni Retnaningtyas ◽  
Diyanul Mustafidah
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Stationary Phase ◽  
Simultaneous Determination ◽  
Dosage Form ◽  
Glacial Acetic Acid ◽  
Ephedrine Hydrochloride ◽  
Tablet Dosage ◽  
Layer Chromatography

The combination of Theophylline and Ephedrine isoften used to induce an additive effect in alleviating thesymptoms of respiratory disorders. This research is aimed todevelop a Thin Layer Chromatography (TLC) densitometrymethodfor the simultaneous determination of Theophyllineand Ephedrine hydrochloride in tablet dosage form. Themethod employed TLC aluminium plates precoated withsilica gel 60F254 as the stationary phase and the mixture ofethyl acetate: glacial acetic acid: aquabidest (11: 5 : 1 v/v)asthe eluen system. For detection of Ephedrinehydrochloride, the TLC plate must be re-eluted with 0,5%ninhydrin solution in aquabidest, and then heated at 105°Cfor 15 min. Quantitative evaluation was performed bymeasuring the absorbance-reflectance of the analyte spot at279 nm and 505 nm for Theophylline and Ephedrinehydrochloride, respectively. validation of the method showthat TLC Densitometryis selective and specific,showed agood linearity, precise, and accurate and can be used forroutine analysis of tablet in industrial quality controllaboratories.Keywords:TLCDensitometryV, alidationT, heophyllineE, phedrinehydrochloride

Separation of dl-2-(3-Phenoxyphenyl) propionic Acid (Fenoprofen) From Its Intermediates by Thin Layer Chromatography

1973 ◽  
Vol 56 (3) ◽  
pp. 656-658
Author(s):  
Rafik H Bishara
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Lower Limit ◽  
Raw Material ◽  
Chemical Constitution ◽  
Tlc Plates ◽  
Rf Values ◽  
Synthetic Intermediates ◽  
Layer Chromatography

Abstract Fenoprofen and its synthetic intermediates are resolved on silica gel F254 TLC plates developed with chloroform-acetic acid (98+2). The interrelationship between the chemical constitution and the Rf values is discussed. The lower limit of detecting the intermediates in Fenoprofen raw material is 1%.

scholarly journals Determination of α-Solanine and α-Chaconine in Potatoes by High-Performance Thin-Layer Chromatography/Densitometry

2000 ◽  
Vol 83 (6) ◽  
pp. 1468-1473 ◽  
Author(s):  
Patricia Bodart ◽  
Charles Kabengera ◽  
Alfred Noirfalise ◽  
Philippe Hubert ◽  
Luc Angenot
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
High Performance ◽  
Ammonium Hydroxide ◽  
Accuracy And Precision ◽  
Saturated Mixture ◽  
Vertical Development ◽  
Layer Chromatography ◽  
Hptlc Method

Abstract A high-performance thin-layer chromatographic (HPTLC) method was used to determine the glycoalkaloids α-solanine and α-chaconine in potatoes. α-Solanine and α-chaconine are extracted from dehydrated potatoes with boiling methanol–acetic acid (95 + 5, v/v). The analytes are separated on a Silica Gel 60 F254 HPTLC plate by a saturated mixture of dichloromethane–methanol–water–concentrated ammonium hydroxide (70 + 30 + 4 + 0.4, v/v), which is used for vertical development of the plate up to a distance of 85 mm. For visualization, the plate is dipped 3 times into a modified Carr-Price reagent, 20% (w/v) antimony(III) chloride in acetic acid–dichloromethane (1 + 3, v/v), and subsequently heated on a hot plate at 105°C for 5 min. The glycoalkaloids all appear as red chromatographic zones on a colorless background. Densitometric quantification is performed at 507 nm by reflectance scanning. After determination of the appropriate response function, the proposed method was validated. Good results with respect to linearity, accuracy, and precision were obtained in the concentration range studied.

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