scholarly journals Thin layer chromatographic compatibility study in preformulation of new transdermal therapeutic systems

2020 ◽  
Vol 66 (1) ◽  
pp. 23-29
Author(s):  
Octavia-Laura Moldovan ◽  
Diana-Nicoleta Brihan ◽  
Nicoleta Todoran ◽  
Aura Rusu
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Mobile Phase ◽  
Glacial Acetic Acid ◽  
Methylene Chloride ◽  
Hydroxyethyl Cellulose ◽  
Compatibility Study ◽  
Chemical Interactions ◽  
Miconazole Nitrate ◽  
Active Substances

AbstractObjective: The compatibility of four binary active substances combinations adapalene – levofloxacin (ADP-LFX), adapalene – miconazole nitrate (ADP-MCZ), levofloxacin – meloxicam (LFX-MLX) and levofloxacin – miconazole nitrate (LFX-MCZ) was analysed to be comprised in new transdermal therapeutic systems. Also, the compatibility of selected active substances and four polymeric excipients (hydroxypropyl methyl-cellulose - HPMC 15000, hydroxypropyl methylcellulose - HPMC E5, ethyl cellulose - EC 10, and hydroxyethyl cellulose – HEC) was studied.Methods: Thin layer chromatographic method (TLC) and four selected mobile phases were used. On the plate (in situ) were obtained the binary combinations (active substances and active substance-polymer).Results: A good compatibility of ADP-LFX was found using ammonia : methanol : acetonitrile : methylene chloride 2:4:1:4 mobile phase. Using chloroform : acetone : glacial acetic acid 34:4:3 on the chromatogram of ADP-MCZ, only ADP spots appeared but without changes in the shape of the spots and Rf values. Any modifications of LFX and MLX spots (from LFX-MLX mixture) had been observed using toluene : glacial acetic acid : methanol 11:1:0.5 mobile phase, although LFX spots have remained on the baseline. Only LFX spots were visible from LFX-MLX and LFX-MCZ mixtures (ammonia : methanol : acetonitrile : methylene chloride 2:4:1:4 mobile phase). Distinctive spots were observed for ADP, LFX and MLX with variable results from no chemical interactions to limited chemical interactions when the compatibility with polymers was verified.Conclusions: ADP-LFX and LFX-MLX mixtures were found to be compatible. ADP with HPMC polymers and LFX with HPMC E5 and HEC had presented excellent compatibility; for the other binary combinations, different analytical methods will be necessary.

Comparative Studies of Thin Layer Chromatographic Systems for the Separation and Identification of Photoalteration Products of Parathion

1973 ◽  
Vol 56 (2) ◽  
pp. 338-340
Author(s):  
Ronald L Joiner ◽  
Karl P Baetcke
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Petroleum Ether ◽  
Infrared Spectra ◽  
Ethyl Ether ◽  
Comparative Studies ◽  
Glacial Acetic Acid ◽  
Ammonium Hydroxide ◽  
Rf Values

Abstract Twelve thin layer chromatographic systems were compared for the separation of photoalteration products of parathion. Rf values for each component in each system are presented. Infrared spectra confirmed the identity of separated components. The following 4 systems were designated superior and gave excellent separation of the components: petroleum ether-ethyl ether-glacial acetic acid (80+15+5 and 50+45+5), methanol-chloroform-ammonium hydroxide (24+75+3.5), and methanol-chloroform-petroleum ether (10+20+70).

scholarly journals ANALYTICAL METHOD OPTIMIZATION AND VALIDATION OF GLIBENCLAMIDE AND METFORMIN HYDROCHLORIDE IN DIABETIC HERBS PRODUCT BY THIN-LAYER CHROMATOGRAPHY-DENSITOMETRY

2020 ◽  
pp. 83-87
Author(s):  
BAITHA PALANGGATAN MAGGADANI ◽  
NOVIANI SUGIANTO ◽  
HAYUN
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Analytical Method ◽  
Mobile Phase ◽  
Glacial Acetic Acid ◽  
Correlation Coefficients ◽  
Metformin Hydrochloride ◽  
Accuracy And Precision ◽  
Method Optimization ◽  
Layer Chromatography

Objective: This study aimed to develop and validate an analytical method using thin-layer chromatography (TLC)-densitometry for detecting thepresence of glibenclamide and metformin hydrochloride in herbal diabetic products.Methods: The jamu samples were extracted using methanol, followed by analysis using TLC-densitometry with methanol-aquades-glacial acetic acid(9:1:0.25) as the mobile phase at a wavelength of 237 nm.Results: From the validation results, the correlation coefficients for glibenclamide and metformin hydrochloride were determined to be 0.9998and 0.9981, respectively. The limits of detection and quantitation for glibenclamide were 49.97 μg/mL and 66.55 μg/mL, respectively, while thoseof metformin hydrochloride were 74.75 μg/mL and 249.25 μg/mL, respectively. The method developed in this study met the criteria of selectivity,accuracy, and precision. From seven jamu samples tested, four tested positive for glibenclamide, with sample 1=4.9522%, sample 2=4.1495%, sample3=4.2578%, and sample 4=4.9412%.Conclusion: None of the jamu samples contained metformin hydrochloride.

Susceptibility of Strawberries, Blackberries, and Cherries to Aspergillus Mold Growth and Aflatoxin Production

1982 ◽  
Vol 65 (3) ◽  
pp. 659-664 ◽  
Author(s):  
Gerald C Llewellyn ◽  
Thomas Eadie ◽  
William V Dashek
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mycelial Growth ◽  
Methylene Chloride ◽  
Aflatoxin Production ◽  
Solvent System ◽  
Mold Growth ◽  
Tlc Plates ◽  
Layer Chromatography

Abstract The susceptibility of blackberries, cherries, and strawberries to Aspergillus growth and aflatoxin production has been examined. Three aflatoxigenic isolates of Aspergillus, A. flavus ATCC 15548 and NRRL 3251 as well as A. parasiticus NRRL 2999, were cultured on homogenates of the fruits for 14 days at 28 ± 2°C. Percent mycelial growth and spore infestation were determined each day with a calibrated grid. At day 14 each culture was frozen at –5°C until aflatoxins were extracted with methylene chloride and water. Aflatoxins were separated by thin layer chromatography (TLC) with benzene-methanol-acetic acid (90 + 5 + 5). This extraction and solvent system provided satisfactory separations of the aflatoxins and was free of background interference on the TLC plates. Although all fruits served as substrates for both Aspergillus growth and aflatoxin production, cherries appeared to be a more favorable substrate than did blackberries, and the latter was more favorable than strawberries. Whereas A. flavus produced both B1 and G1 on all substrates, it yielded B2 and G2 only on cherries. Although A. parasiticus NRRL 2999 synthesized B1, B2, G1, and G2 on both blackberries and cherries, no aflatoxins were detected on strawberries. In contrast, A. flavus NRRL 3251 failed to produce detectable levels of aflatoxin on any substrate. All substrates supported both mycelial growth and subsequent sporulation with cherries > blackberries > strawberries.

European Taxa of Saxicolous Lecidella Containing Chloroxanthones:Identification of Patterns Using Thin Layer Chromatography

1992 ◽  
Vol 24 (4) ◽  
pp. 383-397 ◽  
Author(s):  
Ch. Leuckert ◽  
J.-G. Knoph
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Glacial Acetic Acid ◽  
Uv Light ◽  
Rf Values ◽  
Layer Chromatography ◽  
Spray Reagents

AbstractA method using TLC to recognize compound patterns consisting almost entirely of chloroxanthones in European saxicolous species of Lecidella is described. These are necessary for diagnosis of the taxa studied and of their chemotypes. Two solvents with complementary separatingqualities were required: C, toluene-glacial acetic acid (100:15); J, dichloromethane-acetone (4:1). Although the Rf values in solvent J strongly depend on the concentration of the substances, it is very suitable for identification because the xanthones in this system reveal distinct and characteristic fluorescence colours in UV light, simplifying interpretation enormously. No spray reagents are used. Identifications are made by co-chromatography with authentic lichencompounds

scholarly journals A Rapid Thin Layer Chromatographic Method for Simultaneous Screening of Albendazole and Ivermectin in Formulations Prescribed for Human or Veterinary Use

2019 ◽  
Vol 31 (4) ◽  
pp. 896-900
Author(s):  
R.P. Pawar ◽  
P. Mishra ◽  
A. Durgbanshi ◽  
D. Bose
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Pharmaceutical Preparations ◽  
Cost Effective ◽  
Dosage Forms ◽  
Thin Layer Chromatographic Plate ◽  
Rf Values ◽  
Chromatographic Plate

An easy and selective thin layer chromatographic method has been developed and experimentally validated for the simultaneous screening of most commonly used anthelmintic drugs i.e. albendazole and ivermectin. Separation of these compounds was attained on silica gel 60 F254 pre-coated thin layer chromatographic plate using an optimized mobile phase of diisopropyl ether:ethyl acetate:glacial acetic acid in the ratio of 7:3:0.1 (v/v), respectively at pH 3.5. The calculated Rf values for albendazole and ivermectin were 0.65 and 0.38, respectively and the LOD was found to be 25 μg/ml and 30 μg/ml for albendazole and ivermectin, respectively. The developed method is selective, sensitive, robust, cost effective, eco-friendly, rapid as well as easy to perform. The developed method was successfully applied for the analysis of albendazole and ivermectin in pharmaceutical preparations marketed as oral suspensions, powder, tablets and injectable of the single or combined dosage forms for human as well as veterinary use. It could also be applied for the simultaneous analysis of both the compounds in other samples.

scholarly journals Influence of Different Chromatographic Conditions on the Level of Detection and Quantitation of Spironolactone by means of TLC-Densitometry

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Małgorzata Dołowy
Keyword(s):  
Quality Control ◽  
Acetic Acid ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Glacial Acetic Acid ◽  
Cost Effective ◽  
Pharmaceutical Formulations ◽  
The One ◽  
Tlc Analysis ◽  
Routine Quality Control

The aim of this study was to estimate the influence of different chromatographic conditions on the limits of detection and limits of quantitation (LODs and LOQs) of spironolactone by means of TLC-densitometry under different chromatographic conditions. A comparison of results obtained showed that the choice of appropriate chromatographic conditions for NP-TLC and RP-TLC analysis with densitometry could effectively decrease the LODs and LOQs of spironolactone. Of all chromatographic systems tested, the best was the one comprising chromatographic plates precoated with a mixture of silica gel 60, kieselguhr F254, and mobile phase A (n-hexane-ethyl acetate-glacial acetic acid, 24.5 : 24.5 : 1, v/v/v). The estimated average LOD and LOQ values were 0.034 and 0.103 μg/spot, respectively. This indicates that the described procedure is sufficiently sensitive for the identification and quantification of spironolactone alone. Thereby, the simple and cost-effective TLC-densitometric method can be utilized for the routine quality control of spironolactone in bulk drugs as well as in simple pharmaceutical formulations.

Iodimetric determinations in organic solvents: Determination of manganese oxidation states in methylene chloride solutions

1990 ◽  
Vol 68 (1) ◽  
pp. 79-81 ◽  
Author(s):  
Joaquin F. Perez-Benito ◽  
Enrique Brillas ◽  
Conchita Arias
Keyword(s):  
Acetic Acid ◽  
Quaternary Ammonium ◽  
Glacial Acetic Acid ◽  
Methylene Chloride ◽  
Oxidation States ◽  
Manganese Oxidation ◽  
Chloride Solutions ◽  
Tetrabutylammonium Iodide ◽  
Organic Solutions

An iodimetric technique has been developed to quantitatively analyze the oxidants present in organic solutions. Addition of excess tetrabutylammonium iodide and excess glacial acetic acid results in the formation of tetrabutylammonium triiodide, whose concentration can be measured spectrophotometrically at 295 or 365 nm. Application of this technique to the determination of the final oxidation state of manganese in permanganate reactions in methylene chloride solutions is described. The equilibrium constant for formation of tetrabutylammonium triiodide in methylene chloride has been measured: K = (3.48 ± 0.12) × 104 at 25.0 °C. Keywords: iodimetric determinations, manganese oxidation states, methylene chloride, quaternary ammonium permanganate, quaternary ammonium triiodide.

scholarly journals Simultaneous Estimation of Four Antitussive Components from Herbal Cough Syrup by HPTLC

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Sharada L. Deore ◽  
Payal S. Jaju ◽  
Bhushan A. Baviskar
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
High Performance ◽  
Glacial Acetic Acid ◽  
Simultaneous Estimation ◽  
Retention Factors ◽  
Ich Guidelines ◽  
Cough Syrup ◽  
Hptlc Method

A new simple, rapid, selective and precise high performance thin layer chromatographic (HPTLC) method has been developed for simultaneous estimation of vasicine, glycyrrhizin, eugenol, and cineole in herbal cough syrup. The retention factors of vasicine, glycyrrhizin, eugenol, and cineole are 0.53, 0.44, 0.75, and 0.77, respectively. Chromatography was performed on 60F254 percolated TLC plate using n-hexane : ethyl acetate : glacial acetic acid (8.5 : 1.0 : 0.5 v/v/v). Methods are validated according to ICH guidelines and can be adopted for the routine analysis of vasicine, glycyrrhizin, eugenol and cineole in herbal cough syrup.

scholarly journals Simultaneous analysis of eprosartan and hydrochlorothiazide in tablet formulation by High- Performance thin layer chromatography with ultraviolet absorption densitometry

2021 ◽  
Vol 8 (3) ◽  
pp. 123-128
Author(s):  
Vrushali Tambe ◽  
Vijaya Vichare ◽  
Anupa Wagh ◽  
Surekha Wavhal
Keyword(s):  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Glacial Acetic Acid ◽  
Tablet Formulation ◽  
Simultaneous Analysis ◽  
Phase Detection ◽  
Retention Factors ◽  
Layer Chromatography

A new, simple, accurate, and precise high-performance thin-layer chromatographic method has been established for simultaneous analysis of Eprosartan and Hydrochlorothiazide from a tablet formulation. Standard and sample solutions of Eprosartan and Hydrochlorothiazide were applied to precoated 250 μm layer of silica gel G 60 F and the plates were developed with Chloroform: Acetonitrile: Glacial Acetic Acid (7:3:1,v/v/v) as mobile phase. Detection and evaluation of densitograms was performed densitometrically at 254 nm. The linear range was 200-700 ng/band with the retention factors of Eprosartan and Hydrochlorothiazide were 0.26± 0.02 and 0.44±0.02, respectively. The method was validated and successfully used for analysis of the drugs in tablets.

Comparative Study for Selection of the Optimum Mobile Phase for Separation of Caffeine and Paracetamol by Using TLC Chromatographic Method

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Mohammed Gamal ◽  
Ali Turki ◽  
Almonzer Al-Shemari ◽  
Abd Alla Alomari
Keyword(s):  
Acetic Acid ◽  
Literature Review ◽  
Comparative Study ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Glacial Acetic Acid ◽  
Mobile Phases ◽  
Selection Of

Objective. To investigate the best mobile phase for separation of Paracetamol (PAR) and Caffeine (CAF) using TLC method. Methods. Different mobile phases which were mentioned in literature review were tried and retention times for both PAR and CAF were recorded for each experiment separately. Results : It was found that retardation factors for solvent A Rf (PAR) =0,59 and Rf(CAF)=0,90 ; for solvent B Rf(PAR)=0,92 and Rf(CAF)=0,81; Rf(PAR)=0,22 and Rf (CAF)=0,16. Solvent A is composed of n-hexane-ethyl acetate-ethanol (2.5 + 1.5 + 0.4, v/v/v), solvent B is composed of chloroform,ethyl acetate and ammonia (15,0 + 4,3 + 0,3 v/v/v) and solvent C is composed of methanol,glacial acetic acid and water (25,0 +4,3+70,7 v/v/v ). Conclusion: It was found that the best solvent for separation of CAF and PAR in TLC chromatographic method was composed of n-hexane-ethyl acetate-ethanol (2.5 + 1.5 + 0.4, v/v/v).

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