156 Influence of viscosity and fiber on nutrient digestibility and intestinal physiology in growing pigs

Abstract Fiber is known for decreasing nutrient utilization in pigs. The addition of fiber alters diet viscosity, but our understanding of the effect of diet viscosity on nutrient digestibility is limited. This knowledge is necessary for improving dietary fiber utilization and when evaluating alternative feed ingredients. Thirty-six T-cannulated barrows (n = 6/treatment; initial BW = 26.5 ± 3.9 kg) were fed either corn-soybean meal (CSBM) basal diets or CSBM with 30% distillers dried grains with solubles as fiber source (CSBM+DDGS). The viscosity of diets were modified by using non-viscous cellulose (CEL), medium-viscosity carboxymethylcellulose (MCMC), or high-viscosity carboxymethylcellulose (HCMC) to assess viscosity effects on nutrient digestibility and intestinal physiology. After 29 d on diets, ileal digesta were collected to determine viscosity and apparent ileal digestibility (AID). Also, intestinal tissue and contents were harvested to determine morphometry, goblet cell quantitation, and digestive enzymatic activity. Data were analyzed using a linear mixed model with treatments as fixed effects and initial BW as a random effect. Results are shown in Table 1. No interactions were observed between viscosity and diets. Inclusion of MCMC and HCMC increased viscosity of supernatant and whole digesta compared with CEL. Increasing diet viscosity decreased AID of DM and CP in pigs fed MCMC and HCMC compared with pigs fed CEL, respectively. The AID of DM and CP were greater in CSBM than CSBM+DDGS. Inclusion of CMC increased crypt depth in the jejunum and goblet cell area in the ileum compared with pigs fed CEL regardless of basal diet fed. Digesta trypsin activity was not different among treatments, but inclusion of CMC increased amylase activity by 43% in jejunal digesta compared with CEL. The addition of DDGS reduced AID; however, viscosity affected intestinal morphology and function, suggesting that viscosity is the main characteristics of fiber affecting nutrient utilization of pigs.

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Effects of grinding method and particle size of wheat grain on energy and nutrient digestibility in growing and finishing pigs

Translational Animal Science ◽

10.1093/tas/txaa062 ◽

2020 ◽

Vol 4 (2) ◽

pp. 682-693 ◽

Cited By ~ 1

Author(s):

Jesus A Acosta ◽

Amy L Petry ◽

Stacie A Gould ◽

Cassandra K Jones ◽

Charles R Stark ◽

...

Keyword(s):

Particle Size ◽

Fixed Effects ◽

Linear Mixed Model ◽

Random Effect ◽

Nutrient Digestibility ◽

Growing Pigs ◽

Neutral Detergent Fiber ◽

Finishing Pigs ◽

Wheat Grain ◽

Roller Mill

Abstract Feed grains are processed to improve their value in pig diets by exposing kernel contents to enzymatic and microbial action. The objective of this study was to quantify the effect of reducing mean particle size (PS) of wheat grain ground with two different grinding methods (GMs) on the apparent total tract digestibility (ATTD) of nutrients and energy in growing and finishing pigs. Forty-eight barrows were housed in individual pens for 11 d for two periods. Pigs were randomly assigned to a 3 × 2 × 2 factorial experimental design: three target mean PS of wheat grain (300, 500, and 700 µm), two GMs (roller mill and hammermill), and two body weight (BW) periods (growing period; initial BW of 54.9 ± 0.6 kg and finishing period; initial BW of 110.7 ± 1.4 kg). Diets contained one of six hard red wheat grain samples, vitamins, minerals, and titanium dioxide as an indigestible marker. Feed allowance provided 2.5 (for the two lightest pigs in each treatment) or 2.7 (for the remaining six pigs in each treatment) times the estimated daily maintenance energy requirement for each growth stage. Fecal samples were collected for the last 3 d of each period. Data were analyzed as a linear mixed model with pig as a random effect and PS, GM, and BW period and their interactions as fixed effects utilizing the MIXED procedure of SAS. Growing pigs had greater (P < 0.05) ATTD of dry matter (DM), gross energy (GE), N, acid hydrolyzed ether extract (AEE), and neutral detergent fiber (NDF) by lowering mean PS from 700 to 500 μm using either a roller mill or a hammermill. However, digestibility did not increase when PS was reduced from 500 to 300 μm, except for AEE (P < 0.05). Finishing pigs had greater ATTD of DM, GE, N, AEE, and NDF by lowering mean PS with a hammermill from 700 to 500 μm (P < 0.05), but it was greater for 500 μm than for 300 μm (P < 0.05). Using a roller mill reduced the ATTD of DM and NDF by lowering PS from 700 to 300 μm (P < 0.05). The ATTD of GE decreased by lowering PS from 700 to 500 μm with a roller mill (P < 0.05) for finishing pigs. The ATTD of N and AEE for finishing pigs were similar from 700 to 300 μm when ground by a roller mill. These data suggest that the PS that maximized digestibility for a hammermill is 500 μm for both growing and finishing pigs. However, for the roller mill, the PS resulting in the best digestibility were 500 and 700 μm for growing and finishing pigs, respectively.

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132 A longer adaptation time increased xylanases efficacy in improving dry matter and fiber digestibility in the small intestine of growing pigs fed insoluble fiber

Journal of Animal Science ◽

10.1093/jas/skaa054.096 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 52-53

Author(s):

Amy Petry ◽

Stacie Gould ◽

Nichole Huntley ◽

Mike R Bedford ◽

John Patience

Keyword(s):

Small Intestine ◽

Treatment Period ◽

Fixed Effects ◽

Mixed Model ◽

Linear Mixed Model ◽

Random Effect ◽

Nutrient Utilization ◽

Growing Pigs ◽

Adaptation Time ◽

Insoluble Fiber

Abstract The objective of this experiment was to investigate xylanase efficacy and impact of adaptation time on nutrient utilization in the small intestine of growing pigs fed insoluble fiber. Twenty gilts (30.6 ± 0.2 kg BW; n=5 per treatment) were surgically fitted with t-cannulae in the medial jejunum (292 ± 12 cm distal to the pyloric sphincter) and in the terminal ileum, housed individually, and randomly assigned to one of four dietary treatments: a low-fiber control (LF; 7.3% NDF), a 30% corn bran high-fiber control (HF; 27.2% NDF), HF + 100 mg xylanase/kg (HF+XY; Econase XT 25P; AB Vista, Marlborough, UK), and HF + 50 mg arabinoxylan-oligosaccharide/kg (HF+AX; 3-7 degrees of polymerization). Gilts were limit fed for three 17 d periods (P1, P2, or P3). Each period included 5 d of adaptation, 2 d of fecal collections, 3 d of ileal collections, and 3 d of jejunal collections. Data were analyzed as repeated records using a linear mixed model (PROC Mixed, SAS 9.4) with surgery date as a random effect, and treatment, period, and their interaction as fixed effects. There tended to be a treatment by period interaction for the apparent jejunal digestibility (AJD) of DM driven by HF+XY improving digestibility over time when compared to HF. Xylanase improved AJD of DM by 21.9% (12.04% vs. 15.4%) in P2, and 40.5% (12.18% vs. 20.49%) in P3 (Treatment Period, P = 0.054). Xylanase supplementation improved AJD of NDF, compared to HF, in P2 by 36.3% (13.7% vs. 21.5%), and in P3 by 28.6% (17.7 vs 24.8%; Treatment Period, P = 0.031). Supplementing xylanase improved the apparent ileal digestibity (AID) of DM over HF in P1 by 4.4%, in P2 by 3.7% (73.91% vs. 76.2 %), and in P3 by 7.4% (75.04% vs. 80.84%; Treatment Period P=0.043). In conclusion xylanase efficacy improved with increased adaptation time.

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The influence of xylanase on the fermentability, digestibility, and physiochemical properties of insoluble corn-based fiber along the gastrointestinal tract of growing pigs

Journal of Animal Science ◽

10.1093/jas/skab159 ◽

2021 ◽

Author(s):

Amy L Petry ◽

Nichole F Huntley ◽

Michael R Bedford ◽

John F Patience

Keyword(s):

Mixed Model ◽

Linear Mixed Model ◽

Nutrient Digestibility ◽

Growing Pigs ◽

Neutral Detergent Fiber ◽

Adaptation Period ◽

High Fiber ◽

Gross Energy ◽

Digesta Viscosity ◽

The Impact

Abstract In theory, supplementing xylanase in corn-based swine diets should improve nutrient and energy digestibility and fiber fermentability, but its efficacy is inconsistent. The experimental objective was to investigate the impact of xylanase on energy and nutrient digestibility, digesta viscosity, and fermentation when pigs are fed a diet high in insoluble fiber (>20% neutral detergent fiber; NDF) and given a 46-d dietary adaptation period. Three replicates of 20 growing gilts were blocked by initial body weight, individually housed, and assigned to 1 of 4 dietary treatments: a low-fiber control (LF) with 7.5% NDF, a 30% corn bran high-fiber control (HF; 21.9% NDF), HF+100 mg xylanase/kg [HF+XY, (Econase XT 25P; AB Vista, Marlborough, UK)] providing 16,000 birch xylan units/kg; and HF+50 mg arabinoxylan-oligosaccharide (AXOS) product/kg [HF+AX, (XOS 35A; Shandong Longlive Biotechnology, Shandong, China)] providing AXOS with 3-7 degrees of polymerization. Gilts were allowed ad libitum access to fed for 36-d. On d 36, pigs were housed in metabolism crates for a 10-d period, limit fed, and feces were collected. On d 46, pigs were euthanized and ileal, cecal, and colonic digesta were collected. Data were analyzed as a linear mixed model with block and replication as random effects, and treatment as a fixed effect. Compared with LF, HF reduced the apparent ileal digestibility (AID), apparent cecal digestibility (ACED), apparent colonic digestibility (ACOD), and apparent total tract digestibility (ATTD) of dry matter (DM), gross energy (GE), crude protein (CP), acid detergent fiber (ADF), NDF, and hemicellulose (P<0.01). Relative to HF, HF+XY improved the AID of GE, CP, and NDF (P<0.05), and improved the ACED, ACOD, and ATTD of DM, GE, CP, NDF, ADF, and hemicellulose (P<0.05). Among treatments, pigs fed HF had increased hindgut DM disappearance (P=0.031). Relative to HF, HF+XY improved cecal disappearance of DM (162 vs. 98g; P=0.008) and NDF (44 vs. 13g; P<0.01). Pigs fed xylanase had a greater proportion of acetate in cecal digesta and butyrate in colonic digesta among treatments (P<0.05). Compared with LF, HF increased ileal, cecal, and colonic viscosity, but HF+XY decreased ileal viscosity compared with HF (P<0.001). In conclusion, increased insoluble corn-based fiber decreases digestibility, reduces cecal fermentation, and increases digesta viscosity, but supplementing xylanase partially mitigated that effect.

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305 Methane Emissions and Apparent Total Tract Nutrient Digestibility of Feedlot Beef Steers Under Intensive Management with or Without an Added Nutritional Packet

Journal of Animal Science ◽

10.1093/jas/skab235.306 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 166-167

Author(s):

Andrea M Osorio ◽

Kaue T Tonelli Nardi ◽

Igor Gomes Fávero ◽

Kaliu G Scaranto Silva ◽

Kymberly D Coello ◽

...

Keyword(s):

Treatment Period ◽

Repeated Measures ◽

Fixed Effects ◽

Block Design ◽

Random Effect ◽

Methane Emissions ◽

Nutrient Digestibility ◽

Experimental Unit ◽

Beef Steers ◽

Ch4 Production

Abstract The effects of a nutritional packet were evaluated on CH4 emissions and apparent total tract nutrient digestibility of feedlot beef steers. Thirty Angus-crossbred steers (BW = 542 ± 8.4 kg) were used in a randomized complete block design and allocated into pens equipped with SmartFeed (C-Lock; 15 steers/treatment). Steers were consuming a steam-flaked corn-based diet (88% concentrate DM basis) ad libitum for the last 65 d on feed, and received the following treatments: 1) control and 2) a nutritional packet [0.29% DM basis; live yeast (Saccharomyces cerevisiae; 8.7 Log CFU/g); Vitamin C (5.4 g/kg); Vitamin B1 (13.33 g/kg); NaCl (80 g/kg); KCl (80 g/kg)]. Methane emissions and apparent total tract nutrient digestibility were measured during 3 periods with 5-d of collections each. Gas emissions from steers were measured utilizing the SF6 tracer technique. Feed and fecal samples were collected once and twice (0700 h and 1600 h) daily, respectively, to determine digestibility of nutrients using iNDF as an internal marker. Steer was considered the experimental unit. Data were analyzed as repeated measures using the MIXED procedure of SAS with the fixed effects of treatment, period, and their interaction, and the random effect of block. No treatment × period interactions (P ≥ 0.125) were observed for DMI and any of the CH4 production variables (g/day, g/kg BW0.75, g/nutrient intake, and g/nutrient digested). Moreover, treatments did not affect digestibility of DM, OM, or ADF (P ≥ 0.300); however, digestibility of NDF was increased for treated cattle (P = 0.013), which resulted in a tendency (P = 0.098) to decrease CH4 production in g per kg NDF intake and decreased (P = 0.020) grams CH4 per kg NDF digested. The nutritional packet may be altering ruminal fermentation on intensively managed steers and improving fiber digestibility, which can have benefits on CH4 emission intensity.

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Goats Given Transdermal Flunixin Meglumine Displayed Less Pain Behavior After Castration

10.1101/2020.06.04.134049 ◽

2020 ◽

Author(s):

Amanda Lee ◽

Meggan Graves ◽

Andrea Lear ◽

Sherry Cox ◽

Marc Caldwell ◽

...

Keyword(s):

Fixed Effects ◽

Mixed Model ◽

Linear Mixed Model ◽

Body Position ◽

Random Effect ◽

Pain Behavior ◽

Bout Duration ◽

Flunixin Meglumine ◽

Meat Goats ◽

Transdermal Application

AbstractPain management should be utilized with castration to reduce physiological and behavioral changes. Transdermal application of drugs require less animal management and fewer labor risks, which can occur with oral administration or injections. The objective was to determine the effects of transdermal flunixin meglumine on meat goats’ behavior post-castration. Male goats (N = 18; mean body weight ± standard deviation: 26.4 ± 1.6 kg) were housed individually in pens and randomly assigned to 1 of 3 treatments: (1) castrated, dosed with transdermal flunixin meglumine; (2) castrated, dosed with transdermal placebo; and (3) sham castrated, dosed with transdermal flunixin meglumine. Body position, rumination, and head- pressing were observed for 1 h ± 10 minutes twice daily on days −1, 0, 1, 2, and 5 around castration. Each goat was observed once every 5-minutes (scan samples) and reported as percentage of observations. Accelerometers were used to measure standing, lying, and laterality (total time, bouts, and bout duration). A linear mixed model was conducted using GLIMMIX. Fixed effects of treatment, day relative to castration, and treatment*day relative to castration and random effect of date and goat nested within treatment were included. Treatment 1 goats (32.7 ± 2.8%) and treatment 2 goats (32.5 ± 2.8%) ruminated less than treatment 3 goats (47.4 ± 2.8%, P = 0.0012). Head pressing was greater on day of castration in treatment 2 goats (P < 0.001). Standing bout duration was greatest in treatment 2 goats on day 1 post-castration (P < 0.001). Lying bout duration was greatest in treatment 2 goats on day 1 post-castration compared to treatment 1 and treatment 3 goats(P < 0.001). Transdermal flunixin meglumine improved goats’ fluidity of movement post-castration and decreased head pressing, indicating a mitigation of pain behavior.

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Misspecification of the random effect structure: Implications for the linear mixed model

10.17077/67hl-x2l9 ◽

2020 ◽

Author(s):

Brandon LeBeau

Keyword(s):

Fixed Effects ◽

Serial Correlation ◽

Mixed Model ◽

Linear Mixed Model ◽

Type I Error ◽

Random Effect ◽

Correlated Data ◽

Unbiased Estimation ◽

Type I ◽

Nested Data

<p>The linear mixed model is a commonly used model for longitudinal or nested data due to its ability to account for the dependency of nested data. Researchers typically rely on the random effects to adequately account for the dependency due to correlated data, however serial correlation can also be used. If the random effect structure is misspecified (perhaps due to convergence problems), can the addition of serial correlation overcome this misspecification and allow for unbiased estimation and accurate inferences? This study explored this question with a simulation. Simulation results show that the fixed effects are unbiased, however inflation of the empirical type I error rate occurs when a random effect is missing from the model. Implications for applied researchers are discussed.</p>

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Exercise-induced hypoalgesia in women with varying levels of menstrual pain

Scandinavian Journal of Pain ◽

10.1515/sjpain-2018-0020 ◽

2018 ◽

Vol 18 (2) ◽

pp. 303-310 ◽

Cited By ~ 4

Author(s):

Mervyn Travers ◽

Penny Moss ◽

William Gibson ◽

Dana Hince ◽

Sheree Yorke ◽

...

Keyword(s):

Fixed Effects ◽

Mixed Model ◽

Linear Mixed Model ◽

Random Effect ◽

Activity Levels ◽

Menstrual Pain ◽

Isometric Handgrip ◽

Pressure Pain Thresholds ◽

Significant Difference ◽

Exercise Induced

Abstract Background and aims: Exercise-induced hypoalgesia (EIH) is a well-established phenomenon in pain-free individuals that describes a decrease in pain sensitivity after an acute bout of exercise. The EIH response has been demonstrated to be sub-optimal in the presence of persisting pain. Menstrual pain is a common recurrent painful problem with many women experiencing high levels of pain each cycle. However, the EIH response has not been examined in a cohort of women with high levels of menstrual pain. This research aimed to examine whether EIH manifests differently in women with varying levels of menstrual pain. The primary hypothesis was that women with high levels of menstrual pain would demonstrate compromised EIH. Secondary aims were to explore relationships between EIH and emotional state, sleep quality, body mass index (BMI) or physical activity levels. Methods: Pressure pain thresholds (PPT) were measured in 64 participants using a digital handheld algometer before and after a submaximal isometric-handgrip exercise. EIH index was compared between low (VAS 0–3), moderate (VAS 4–7) and high (VAS 8–10) pain groups, using a linear mixed model analysis with participant as a random effect, and site, menstrual pain category and the interaction between the two, as fixed effects. Results: EIH was consistently induced in all groups. However, there was no statistically significant difference between the pain groups for EIH index (p=0.835) or for any co-variates (p>0.05). Conclusions: EIH was not found to differ between women who report regular low, moderate or high levels of menstrual pain, when measured at a point in their menstrual cycle when they are pain free. Implications: This study provides insight that EIH does not vary in women with differing levels of menstrual pain when they are not currently experiencing pain. The current findings indicate that, although menstrual pain can involve regular episodes of high pain levels, it may not be associated with the same central nervous system dysfunctions as seen in sustained chronic pain conditions.

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Detection of Methane Eructation Peaks in Dairy Cows at a Robotic Milking Station Using Signal Processing

Animals ◽

10.3390/ani12010026 ◽

2021 ◽

Vol 12 (1) ◽

pp. 26

Author(s):

Ali Hardan ◽

Philip C. Garnsworthy ◽

Matt J. Bell

Keyword(s):

Signal Processing ◽

Fixed Effects ◽

Mixed Model ◽

Linear Mixed Model ◽

Random Effect ◽

Air Sampling ◽

Analysis Software ◽

Sampling Volume ◽

Ch4 Concentration ◽

Robotic Milking

The aim of this study was to investigate the use of signal processing to detect eructation peaks in CH4 released by cows during robotic milking, and to compare recordings from three gas analysers (Guardian SP and NG, and IRMAX) differing in volume of air sampled and response time. To allow comparison of gas analysers using the signal processing approach, CH4 in air (parts per million) was measured by each analyser at the same time and continuously every second from the feed bin of a robotic milking station. Peak analysis software was used to extract maximum CH4 amplitude (ppm) from the concentration signal during each milking. A total of 5512 CH4 spot measurements were recorded from 65 cows during three consecutive sampling periods. Data were analysed with a linear mixed model including analyser × period, parity, and days in milk as fixed effects, and cow ID as a random effect. In period one, air sampling volume and recorded CH4 concentration were the same for all analysers. In periods two and three, air sampling volume was increased for IRMAX, resulting in higher CH4 concentrations recorded by IRMAX and lower concentrations recorded by Guardian SP (p < 0.001), particularly in period three, but no change in average concentrations measured by Guardian NG across periods. Measurements by Guardian SP and IRMAX had the highest correlation; Guardian SP and NG produced similar repeatability and detected more variation among cows compared with IRMAX. The findings show that signal processing can provide a reliable and accurate means to detect CH4 eructations from animals when using different gas analysers.

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163 Annual differences in fecal egg counts in a forage-based ram test

Journal of Animal Science ◽

10.1093/jas/skz258.354 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 172-172

Author(s):

Morgan Van Davelaar ◽

David R Notter ◽

D Lee Wright ◽

Anna M Zajac ◽

Scott P Greiner ◽

...

Keyword(s):

Fixed Effects ◽

Mixed Model ◽

Linear Mixed Model ◽

Negative Binomial ◽

Random Effect ◽

Summer Precipitation ◽

Parasite Load ◽

Least Square ◽

Birth Type ◽

Normally Distributed

Abstract The objective was to determine the magnitude of yearly differences in parasite load in growing ram lambs. Data were obtained from the Southwest Virginia Agriculture Research and Extension Center in Glade Spring, VA. The center conducts a forage-based ram growth test during the summer where rams also undergo a parasite challenge. Data consisted of 488 Katahdin rams tested from 2012 to 2018. Rams were dewormed at delivery, and at the start of the test, each ram received an oral dose of 5,000 H. contortus larvae adjusted for body weight. Fecal egg count was measured 70 d later when rams were on average (SD) 200 (18) d old. Fecal egg counts were not normally distributed. The Box-Cox procedure indicated that a log transformation was appropriate, but the residuals were not normally distributed for several linear models. A zero-inflated negative binomial generalized linear mixed model was used for data analysis with the glmmTMB package in R. The model included fixed effects of centered and scaled weight, centered and scaled age, year, birth type, and rearing type, and the random effect of consignor. Birth type, rear type, and age were not significant (P > 0.10). The least square mean (SE) fecal egg counts by year were 344 (118) for 2012, 623 (214) for 2013, 574 (195) for 2014, 1,125 (409) for 2015, 745 (253) for 2016, 408 (142) for 2017, and 239 (86) for 2018. Differences in summer precipitation could affect average parasite load. Despite similar total summer precipitation, 2012 had 1 extremely wet month whereas, 2015 had consistent precipitation throughout the summer. We conclude that producers should not compare fecal egg counts across years because the overall average may be multiple-fold different from year to year. Ranking rams within year will be more effective to select for improved parasite resistance.

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Effect of probiotic Pediococcus acidilactici FT28 on growth performance, nutrient digestibility, health status, meat quality, and intestinal morphology in growing pigs

Veterinary World ◽

10.14202/vetworld.2018.1669-1676 ◽

2018 ◽

pp. 1669-1676 ◽

Cited By ~ 2

Author(s):

Mamata Joysowal ◽

B. N. Saikia ◽

Runjun Dowarah ◽

S. Tamuly ◽

D. Kalita ◽

...

Keyword(s):

Health Status ◽

Growth Performance ◽

Meat Quality ◽

Nutrient Digestibility ◽

Growing Pigs ◽

Intestinal Morphology ◽

Pediococcus Acidilactici ◽

N Retention ◽

Fermented Feed ◽

G Ratio

Aim: The experiment was conducted to evaluate the effect of swine-origin probiotic Pediococcus acidilactici FT28 on growth, nutrient utilization, health status, meat quality and intestinal morphology in growing female pigs. Materials and Methods: Pigs (n=27) were distributed into three groups (3 replicates of 3 each) and supplemented with basal diet either without probiotics (C) or with a probiotic of dairy-based (Lactobacillus acidophilus NCDC-15; TLact) or swine based (P. acidilactici FT28; TPedic). The probiotics were fed as fermented feed at 200 g/pig/day. At the end of the trial, six pigs from each group were selected for metabolism trial and then sacrificed to determine meat quality and intestinal morphology. Results: Supplementation of both probiotics improved growth performance, whereas feed intake, digestibility of CP and N retention were better (p<0.05) in P. acidilactici FT28-fed group. However , the digestibility of dry matter (DM), organic matter (OM), ether extracts (EE), crude fibre and nitrogen free extract did not show any significant effect on probiotic supplementation. The serum A: G ratio, triglyceride, and cholesterol level were also improved (p<0.05) in TPedic group compared to other treatment groups. Both probiotic supplementations showed lower (p<0.05) serum glucose level with similar protein and albumin value, which indicated good utilization of feed as well as health status of growing pigs. Dressing percentage, vital organ weight, and EE of loin meat were higher (p<0.05) in probiotic-supplemented groups compared to control. However, P. acidilactici FT28-fed animals showed higher (p<0.05) CP and total ash percentage of meat without affecting pH, water holding capacity, and extract release volume of loin muscle. The villi height and crypt depth were better in both supplemented groups compared to control. Conclusion: Results of the present study revealed that P. acidilactici FT28 could serve as better probiotic source in swine production for the better utilization of CP and N-retention in meat with improved health status and intestinal morphology.

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