scholarly journals 282 Effect of formulating diets based on a ratio between STTD Ca and STTD P and the inclusion of phytase on the calcium and phosphorus balance of growing pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 103-104
Author(s):  
Vanessa Lagos ◽  
Mike R Bedford ◽  
Hans H Stein
Keyword(s):  
Block Design ◽  
Random Effect ◽  
Growing Pigs ◽  
Diet Formulation ◽  
Randomized Complete Block Design ◽  
Microbial Phytase ◽  
P Utilization ◽  
P Balance ◽  
Main Effects ◽  
P Absorption

Abstract An experiment was conducted to test the hypothesis that formulating diets for pigs based on a ratio between standardized total tract digestible (STTD) Ca and STTD P instead of total Ca and STTD P increases the efficiency of Ca and P utilization. Forty barrows (59.4 ± 3.8 kg) were individually housed in metabolism crates and allotted to 4 corn-soybean meal diets and 2 periods of 11 d in a randomized complete block design. Diets were formulated using a 2 × 2 factorial design with 2 Ca requirement estimates (total Ca or STTD Ca) and 2 inclusion levels of microbial phytase (0 or 500 units/kg). Phytase was assumed to release 0.11% STTD P and 0.16% total Ca. Diets were formulated based on requirements for total Ca and STTD P and the ratio between STTD Ca and STTD P was 1.25:1 in diets formulated based on STTD Ca. Fecal and urine samples were collected from feed provided from d 6 to 9. Data for Ca and P balance were analyzed using a model that included the main effects of diet formulation and phytase level, the interaction between main effects, and the random effect of period. Interactions (P < 0.05) between diet formulation and phytase level were observed for Ca intake, Ca in feces, Ca digestibility, Ca retained as a percentage of intake, P digestibility, P absorbed, and P in urine (Table 1). Despite being provided less (P < 0.05) Ca, pigs fed diets formulated based on STTD Ca did not absorb or retain less Ca than pigs fed total Ca diets, but they absorbed more (P < 0.05) P than pigs fed diets formulated based on total Ca. In conclusion, by formulating diets based on values for STTD Ca, P absorption was increased confirming detrimental effects of oversupplying Ca.

Formulation of diets for pigs based on a ratio between digestible calcium and digestible phosphorus results in reduced excretion of calcium in urine without affecting retention of calcium and phosphorus compared with formulation based on values for total calcium

2021 ◽  
Author(s):  
L Vanessa Lagos ◽  
Su A Lee ◽  
Mike R Bedford ◽  
Hans H Stein
Keyword(s):  
Detrimental Effect ◽  
Block Design ◽  
Total Calcium ◽  
Diet Formulation ◽  
Randomized Complete Block Design ◽  
Microbial Phytase ◽  
P Utilization ◽  
Calcium And Phosphorus ◽  
P Absorption ◽  
P Retention

Abstract An experiment was conducted to test the hypothesis that formulating diets for pigs based on a ratio between standardized total tract digestible (STTD) Ca and STTD P instead of total Ca and STTD P does not decrease Ca retention, but increases P utilization. Forty barrows (59.4 ± 3.8 kg) were individually housed in metabolism crates and allotted to 4 corn-soybean meal-based diets in a randomized complete block design with 2 blocks and 5 pigs per diet in each block. Diets were formulated using a 2 × 2 factorial design with 2 diet formulation principles (total Ca or STTD Ca) and 2 inclusion levels of microbial phytase (0 or 500 units per kg of feed). Phytase was assumed to release 0.11% STTD P and 0.16% total Ca. Diets were formulated based on requirements for total Ca and STTD P or a ratio between STTD Ca and STTD P of 1.25:1. Diets were fed for 11 d and fecal and urine samples were collected from feed provided on d 6 to 9. Interactions (P < 0.05) between diet formulation principle and phytase level were observed for Ca intake, Ca in feces, Ca absorbed, Ca retained, P digestibility, P absorbed, and P in urine. Phytase increased (P < 0.05) the digestibility of Ca in both total Ca and STTD Ca diets. Without phytase, Ca intake, Ca in feces, and Ca absorbed was greater (P < 0.05) from pigs fed total Ca diets than from pigs fed STTD Ca diets, but P absorbed, P digestibility, and P in urine was greater (P < 0.05) from pigs fed STTD Ca diets than from pigs fed total Ca diets. However, in the presence of phytase, no differences between diet formulation principles were observed in these variables. Regardless of phytase, Ca in urine was lower (P < 0.05) from pigs fed STTD Ca diets than from pigs fed total Ca diets. There were no differences in Ca retention between pigs fed STTD Ca diets and total Ca diets, but pigs fed total Ca diets retained less (P < 0.05) Ca if diets contained phytase. No differences in P retention were observed between diet formulation principles, but pigs fed non-phytase diets retained more (P < 0.05) P than pigs fed diets with phytase. In conclusion, because diets formulated based on STTD Ca contain less Ca than total Ca diets, pigs fed STTD Ca diets excreted less Ca in urine, but retention of Ca was not affected. Formulating non-phytase diets based on STTD Ca instead of total Ca increased P absorption, which confirms the detrimental effect of excess Ca on P digestibility. However, P retention was not improved if pigs were fed STTD Ca diets.

scholarly journals A new source of high-protein distillers dried grains with solubles (DDGS) has greater digestibility of amino acids and energy, but less digestibility of phosphorus, than de-oiled DDGS when fed to growing pigs

2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Minoy Cristobal ◽  
Jessica P Acosta ◽  
Su A Lee ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Crude Protein ◽  
Block Design ◽  
Latin Square ◽  
Sole Source ◽  
Growing Pigs ◽  
Ileal Digestibility ◽  
Distillers Dried Grains ◽  
Randomized Complete Block Design ◽  
Microbial Phytase

Abstract AbstractThree experiments were conducted to test the hypothesis that standardized ileal digestibility (SID) of amino acids (AA), concentration of metabolizable (ME), and standardized total tract digestibility (STTD) of P in a new source of distillers dried grains with solubles (DDGS; ProCap DDGS) are greater than in conventional de-oiled DDGS. In experiment 1, nine barrows (initial BW: 67.2 ± 6.4 kg) with a T-cannula in the distal ileum were allotted to a triplicated 3 × 3 Latin square design with three diets and three periods for a total of nine replicate pigs per diet. Two diets included ProCap DDGS or de-oiled DDGS as the sole source of crude protein (CP) and AA. An N-free diet was used to determine the basal endogenous losses of CP and AA. Ileal digesta were collected on days 5 and 6 of each period after 4 d of adaptation to diets. Results from experiment 1 indicated that ProCap DDGS contained more CP and AA compared with de-oiled DDGS. The SID of all AA in ProCap DDGS was greater (P < 0.001) compared with de-oiled DDGS with the exception that the SID of Pro was not different between the two sources of DDGS. In experiment 2, 24 growing barrows (initial BW: 32.7 ± 3.1 kg) were housed individually in metabolism crates and used in a randomized complete block design and fed a corn-based diet or two diets containing corn and each source of DDGS with eight replicate pigs per diet. Fecal and urine samples were collected for 4 d after 7 d of adaptation. Results from experiment 2 indicated that concentration of ME in ProCap DDGS was greater (P < 0.05) compared with corn or de-oiled DDGS. In experiment 3, 32 growing barrows (initial BW: 20.2 ± 0.9 kg) were placed in metabolism crates and allotted to four diets with eight pigs per diet using a 2 × 2 factorial treatment arrangement. The de-oiled DDGS and ProCap DDGS were both included in a diet without microbial phytase and a diet with microbial phytase (500 units/kg diet). Pigs were adapted to the diets for 5 d and fecal samples were collected for 4 d. Results from experiment 3 indicated that inclusion of phytase in the diet containing ProCap DDGS increased (P < 0.05) the STTD of P, but addition of phytase to the de-oiled DDGS diet did not increase STTD of P (interaction, P < 0.001), but the STTD of P was greater (P < 0.05) in de-oiled DDGS compared with ProCap DDGS. In conclusion, ProCap DDGS has greater SID of AA and contains more ME, but has reduced STTD of P compared with conventional de-oiled DDGS.

171 Effects of Dietary Valine, Isoleucine, and Tryptophan Supplementations to Diets Containing Excess Leucine on Nitrogen Balance of Growing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 61-61
Author(s):  
Woongbi Bi Kwon ◽  
Jose A Soto ◽  
Hans H Stein
Keyword(s):  
Body Weight ◽  
Crude Protein ◽  
Random Effect ◽  
Basal Diet ◽  
Growing Pigs ◽  
Negative Effects ◽  
Initial Body Weight ◽  
N Retention ◽  
Biological Value ◽  
Main Effects

Abstract An experiment was conducted to test the hypothesis that increasing concentrations of dietary Val, Ile, or Trp alone or in combination will alleviate negative effects of excess dietary Leu on N balance of growing pigs. Seventy-two barrows (initial body weight: 33.9 ± 2.6 kg) were housed in metabolism crates and randomly assigned to 1 of 8 diets and 3 blocks with 3 pigs per diet in each block in a 12-d experiment. Treatments were arranged in a 2 × 2 × 2 factorial with the main effects of L-Val (0 or 0.1%), L-Ile (0 or 0.1%), and L-Trp (0 or 0.05%) that were added to a basal diet containing corn and a high-protein corn product (48% crude protein; 5.9% Leu). The basal diet contained 1.00 % standardized ileal digestible (SID) Lys and 171% SID Leu:Lys. Data were analyzed using the PROC MIXED of SAS with concentrations of Val, Ile, and Trp, and all interactions as main effects and replicate as a random effect. No 3-way interactions were observed (Table 1). Results indicated that fecal N output increased if Ile was added to diets without added Val, but that was not the case if Val was added (interaction, P < 0.05). Addition of Ile to diets reduced N retention, but N retention increased with Trp addition to diets without Val addition, but not if Trp was added to diets with added Val (interaction, P < 0.05). The biological value of protein increased if Trp was added to diets without addition of Ile, but if Ile was added, Trp addition did not increase the biological value of protein (interaction, P < 0.05). In conclusion, adding Ile to a diet with excess Leu reduced N retention, but if Trp was added alone or in combination with Ile or Val, N retention increased.

scholarly journals 281 Young Scholar Presentation: Digestibility of calcium and phosphorus in feed ingredients fed to gestating sows and growing pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 111-111
Author(s):  
Su A Lee ◽  
Hans H Stein
Keyword(s):  
Serum Biomarkers ◽  
Growing Pigs ◽  
Hormonal Control ◽  
Late Gestation ◽  
Young Scholar ◽  
Diet Formulation ◽  
Microbial Phytase ◽  
The Impact ◽  
Ca:P Ratio

Abstract In practical diet formulation, values for standardized total tract digestibility (STTD) of Ca and P obtained in growing pigs are also applied to sows. However, gestating sows have reduced digestibility and retention of Ca and P compared with growing pigs, and the impact of microbial phytase on the digestibility of P and Ca is much less in sows than in growing pigs. Applying STTD values for Ca and P obtained in growing pigs to diets for gestating sows, therefore, results in an overestimation of the absorbed Ca and P in sows (Table 1). Further research, however, indicated that the digestibility of Ca and P in late gestating sows is greater than in sows in early or mid-gestation and retention of Ca and P was greater in late-gestation compared with earlier gestation periods, which indicates that digestion and absorption of Ca and P may be under hormonal control in sows (Table 2). It was also demonstrated that a wide Ca:P ratio decreased P digestibility in both growing pigs and sows in late-gestation, which demonstrates the need for not overfeeding STTD Ca. In follow-up research, it was demonstrated that several serum biomarkers may be used to predict if a sow is in a positive or a negative Ca and P state, but more research is needed to quantify this effect and to determine if biomarkers can be used in Ca and P requirement experiments. In conclusion, gestating sows have much lower digestibility of Ca and P than growing pigs, which demonstrates that digestibility values obtained in growing pigs cannot be used to accurately formulate diets for gestating sows. Likewise, effects of microbial phytase on digestibility of Ca and P are much less predictable in gestating sows than in growing pigs and phytase effects in sows are much smaller than in growing pigs.

Evaluation of sugarcane genotypes with respect to sucrose yield across three crop cycles using GGE biplot analysis

2021 ◽  
pp. 1-13
Author(s):  
Aliya Momotaz ◽  
Per H. McCord ◽  
R. Wayne Davidson ◽  
Duli Zhao ◽  
Miguel Baltazar ◽  
...  
Keyword(s):  
Block Design ◽  
Genotype By Environment Interaction ◽  
Environment Interaction ◽  
Gge Biplot ◽  
Genotype By Environment ◽  
Biplot Analysis ◽  
Genotype Environment Interaction ◽  
Area 5 ◽  
Randomized Complete Block Design ◽  
Main Effects

Summary The experiment was carried out in three crop cycles as plant cane, first ratoon, and second ratoon at five locations on Florida muck soils (histosols) to evaluate the genotypes, test locations, and identify the superior and stable sugarcane genotypes. There were 13 sugarcane genotypes along with three commercial cultivars as checks included in this study. Five locations were considered as environments to analyze genotype-by-environment interaction (GEI) in 13 genotypes in three crop cycles. The sugarcane genotypes were planted in a randomized complete block design with six replications at each location. Performance was measured by the traits of sucrose yield tons per hectare (SY) and commercial recoverable sugar (CRS) in kilograms of sugar per ton of cane. The data were subjected to genotype main effects and genotype × environment interaction (GGE) analyses. The results showed significant effects for genotype (G), locations (E), and G × E (genotype × environment interaction) with respect to both traits. The GGE biplot analysis showed that the sugarcane genotype CP 12-1417 was high yielding and stable in terms of sucrose yield. The most discriminating and non-representative locations were Knight Farm (KN) for both SY and CRS. For sucrose yield only, the most discriminating and non-representative locations were Knight Farm (KN), Duda and Sons, Inc. USSC, Area 5 (A5), and Okeelanta (OK).

scholarly journals PSIV-16 Effect of a 1-monoglyceride, 1,2-diglyceride of butyric-, caproic- and caprilic acid, and Avilamycin on performance and intestinal E. coli when fed to piglets challenged with E. coli

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 188-189
Author(s):  
Michael Coelho ◽  
Peter Ader
Keyword(s):  
Caprylic Acid ◽  
Block Design ◽  
Random Effect ◽  
Fixed Effect ◽  
Experimental Unit ◽  
Infection Model ◽  
E Coli ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
T1 And T2

Abstract This study examined the effect of 1-monoglycerides and 1,2-diglycerides of butyric-, caproic- and caprylic acid (SILOHealth™ 104L [monoglycerides]) and avilamycin on day 14 and 21 post weaning performance of E. coli (GN-Ent and O157:H7) day 5 challenged piglets fed corn/soy diets that met or exceeded current NRC (2012) recommendations. A total of 96, Choice Genetics CG32, piglets were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (4 piglets/pen x 3 treatments x 8 replications). Pigs were blocked by sex and weight and were orally dosed with 5 ml (1x109 CFU/g, strain GN-Ent and O157:H7) E. coli d 5 after weaning (Ec). The treatment were: NC with Ec; T1=NC + 3 kg/MT monoglycerides; T2=NC+ 80 ppm avilamycin. Least significant difference was used to compare means of treatment. Mortalities and pen removals were weighed and recorded. The E. coli infection model was effective in causing E. coli mortality of 25% at d14 post weaning and 33% at d21 post weaning in NC. T1 and T2 reduced mortality versus NC at d14 (25, 3 and 0%, P < 0.05) and d21 (33, 4 and 0%, P < 0.05), increased ADG at d14 (203, 255 and 259 g, P < 0.05) and d21 (456, 511 and 515 g, P < 0.05), decreased FCR at d21 (1.90, 1.85 and 1.83, P < 0.05), and decreased intestinal E. coli, log 10 of CFU/g, versus NC (4.1, 2.2 and 2.1, P < 0.05). Monoglycerides and avilamycin performance, mortality and intestinal E. coli log 10 were not significantly different at P < 0.05. In conclusion, the use of monoglycerides improved ADG, reduced FCR, reduced mortality and reduced intestinal E. coli log 10 at day 14 and 21 post weaning versus NC and monoglycerides were not significant different (P < 0.05) than avilamycin.

scholarly journals PSVIII-5 Determination of true digestibility and the endogenous outputs of magnesium in corn for growing pigs by using the regression analysis technique

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 285-285
Author(s):  
Hongzhi Wu ◽  
Xindi Yin ◽  
Zeyu Yang ◽  
Tania Archbold ◽  
Wenyi Fan ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Bone Mineralization ◽  
Block Design ◽  
Growing Pigs ◽  
Linear Relationships ◽  
Diet Formulation ◽  
Analysis Technique ◽  
Dietary Magnesium ◽  
Corn Grain

Abstract Dietary magnesium (Mg) is essential to bone mineralization. Supplemental Mg is typically not considered in commercial swine diets by assuming high bioavailability of Mg from bulky feed ingredients such as corn grain. The objectives of this study were to determine true ileal and fecal digestibility and the endogenous losses of Mg associated with corn in growing pigs by the regression analysis technique. A total of 48 barrows, with an average initial body weight (BW) 32 kg, were randomly assigned to 6 grower pig diets and were fed close to ad libitum for 10 d, with 8-d adaptation and 2-d collection fecal and the terminal ileal digesta samples, according to a randomized complete block design. Six cornstarch-based diets, containing 6 levels of Mg at 0.22, 0.32, 0.38, 0.51, 0.71 and 0.79 g/kg dry matter intake (DMI) of diets, were formulated from corn. There were linear relationships (P &lt; 0.05), expressed as g/kg DMI, between the ileal and fecal outputs of Mg and the total intake of dietary Mg, suggesting that true ileal and fecal Mg indigestibility values (94.8±12.5 vs. 89.2±17.7%); and the ileal and fecal endogenous Mg outputs (0.16±0.02 vs. 0.21±0.11, g/kg DMI of diets) associated with corn could be estimated by the regression analysis. Our results have shown that Mg associated with conventional corn grain was very poorly digested and the gastrointestinal endogenous fecal loss of Mg was significant in the grower pig. Thus, Mg bioavailability in feeds for pigs should be assessed and supplemental of Mg may be warranted in swine diet formulation.

scholarly journals 126 Energy (ME)-releasing efficacy of graded levels of 6-phytase, Natuphos® E, and a NSPase, Natugrain® TS, in 35 kg pigs fed corn/soy diets

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 70-70
Author(s):  
Michael Coelho ◽  
Robert Jones ◽  
Frank Parks
Keyword(s):  
Phytic Acid ◽  
Block Design ◽  
Random Effect ◽  
Fixed Effect ◽  
Experimental Unit ◽  
Fecal Samples ◽  
Randomized Complete Block Design ◽  
Feed Costs

Abstract This study evaluated the metabolizable (ME) release of graded levels of phytase when fed to 35 kg pigs on mash corn/soy diets formulated to meet or exceed current NRC (2012) recommendations. A total of 280 male (PIC 337 x Camborough) pigs were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (4 pig/pen x 8 treatments x 10 replications). Pigs were blocked by sex and weight. Treatments groups consisted of PC, NC1=PC- 0.17% Ca, - 0.15% aP, -0.02% Na, -200 kcal/kg, T1=NC +1000 FTU/kg phytase+0.15 g/kg NSPase, T2=NC+1500 FTU/kg phytase+0.15 g/kg NSPase, T3=NC+2000 FTU/kg phytase+0.15 g/kg NSPase, T4=NC+2500 FTU/kg phytase+0.15 g/kg NSPase and T5=NC+3000 FTU/kg phytase +0.15 g/kg NSPase. TiO2 was used as the undigestible marker. Feed, rectum grabbed fecal samples were collected at 35 kg pigs. ME was 3407, 3213, 3301, 3329, 3345, 3354 and 3358 Kcal/kg, P < 0.05, respectively. The NC reduced the ME by 194 Kcal/kg versus the PC. 1000, 1500, 2000, 2500 and 3000 FTU/kg released 87, 116, 132, 141 and 145 Kcal/kg, respectively. Least cost formulation reduced net feed costs by $8.63, $9.22, $11.10, $11.90 and $12.39/ton, respectively. In conclusion, Natuphos E by breaking down phytic acid and Natugrain TS by breaking down NSPs released up to 145 Kcal/kg ME in pigs.

scholarly journals 127 A novel find of a Feed NSPase Enzyme, Natugrain® TS, reducing finish pig hemorrhagic bowel syndrome mortality

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 70-71
Author(s):  
Michael Coelho ◽  
Robert Jones ◽  
Frank Parks
Keyword(s):  
Block Design ◽  
Random Effect ◽  
Fixed Effect ◽  
Control Treatment ◽  
Experimental Unit ◽  
Lesion Score ◽  
Intestinal Lesion ◽  
Fecal Samples ◽  
E Coli ◽  
Randomized Complete Block Design

Abstract This study presents a novel find of a Feed NSPase Enzyme, Natugrain TS, reducing finish pig hemorrhagic bowel syndrome (HBS) mortality. A total of 3,750 weaned pigs (28 kg), (PIC 337 x Camborough), were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (25 pigs/pen x 5 treatments x 30 replications). Pigs were blocked by sex and weight and were fed mash corn/soy/DDGs diets formulated to meet or exceed current NRC (2012) recommendations. Treatments were T1= 0 g/MT Natugrain TS, T2= 100 g/MT Natugrain TS, T3= 150 g/MT Natugrain TS, T4= 200 g/MT Natugrain TS and T5= 250 g/MT Natugrain TS. At the finishing phase, Natugrain TS significantly decreased intestinal E. coli log 10 (7.12, 4.95, 4.02, 3.21 and 3.25 log 10, P < 0.05, respectively), significantly decreased intestinal lesion score (0–5 score) (4.21, 4.02, 2.98, 2.25 and 2.26, P < 0.05, respectively). Microbiota was analyzed in fresh, manually collected, rectum fecal samples. Microbiota was separated into prevalent in Natugrain TS control (0) and 200 g/ton. In the control treatment, there was a prevalence of Desulfoyibrio, Oscillospira, Campylobacter, Anearoctruncus, Mogibacterium and Brachyspirae, etc., while in the 200 g/ton treatment there was a prevalence of Synergistales, Burkolderiales, Dehabacteriaceae, Bifidobacterium, Roseburia, Lactobacillus and Bacilli, etc. Natugrain TS significantly decreased mortality (4.8, 3.6, 2.7, 1.9 and 2.1 %, P < 0.05, respectively), significantly decreased specifically HBS mortality (3.50, 1.98, 0.68, 0.40 and 0.46%, P < 0.05, respectively). In conclusion, Natugrain TS changed the microbiome by breaking down arabinoxylans, decreasing intestinal E. coli and intestinal lesion score, changing the microbiota to prevalent Bifidobacterium, Roseburia, Lactobacillus and Bacilli, and Natugrain TS up to 200 g/ton specifically decreasing hemorrhagic bowel syndrome mortality from 3.5% to 0.5%.

scholarly journals Genotype by environment interaction for seeds yield in pea (Pisum sativum L.) using additive main effects and multiplicative interaction model

Euphytica ◽  
2019 ◽  
Vol 215 (11) ◽  
Author(s):  
Jan Bocianowski ◽  
Jerzy Księżak ◽  
Kamila Nowosad
Keyword(s):  
Pisum Sativum ◽  
Block Design ◽  
Interaction Model ◽  
Genotype By Environment Interaction ◽  
Environment Interaction ◽  
Ge Interaction ◽  
Multiplicative Interaction ◽  
Genotype By Environment ◽  
Randomized Complete Block Design ◽  
Main Effects

Abstract The objective of this study was to evaluate the genotype by environment interaction using the additive main effects and multiplicative interaction model for seeds yield of pea cultivars grown in Poland. Twelve pea (Pisum sativum L.) cultivars: Bohun, Boruta, Cysterski, Ezop, Kavalir, Lasso, Medal, Santana, Tarchalska, Terno, Wenus and Zekon were evaluated in 20 environments (ten locations in 2 years). The experiment was laid out as randomized complete block design with three replicates. Seeds yield ranged from 26.10 dt ha−1 (for Wenus in Radostowo 2011) to 79.73 dt ha−1 (for Lasso in Słupia 2010), with an average of 50.70 dt ha−1. AMMI analyses revealed significant genotype and environmental effects as well as genotype-by-environment interaction with respect to seeds yield. In the analysis of variance, 89.19% of the total seeds yield variation was explained by environment, 1.65% by differences between genotypes, and 8.33% by GE interaction. The cultivar Terno is the highest stability. The cultivar Tarchalska is recommended for further inclusion in the breeding program because its stability and the highest averages of seeds yield.

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