PSIV-1 Apparent and Standardized Ileal Digestibility of Amino Acids in Dry Extruded-Expelled Soybean Meal Fed to Growing Pigs Without or With Organic Acids and Multi-Carbohydrase

Abstract Dry extruded-expelled soybean meal (DESBM) is an attractive feed ingredient for swine not only as a protein source but also as a good source of energy because of its high fat content. Apparent (AID) and standardized (SID) ileal amino acid (AA) digestibility in DESBM fed without or with organic acid (OA) and a multicarbohydrase (MC) supplementation to growing pigs were determined. Ten ileal-cannulated barrows (17.3 ± 0.4 kg BW) were randomly assigned to 1 of 5 dietary treatments in a replicated 5 × 4 incomplete Latin square design with 4 periods, each consisting of 5 d of adaptation and 2 d of ileal digesta collection. Four cornstarch-based diets with 44.4% DESBM as the only source of AA, without or with OA and MC supplementation, and a 5% casein-cornstarch diet were used. All diets contained 0.3% titanium dioxide. Data were analyzed using the MIXED model procedures of SAS, with the final model including OA, MC, and the interaction between OA and MC as the main effects. The SID of indispensable AA in DESBM fed to growing pigs were: Arg, 93.2%; His, 85.1%; Ile, 87.1%; Leu, 87.6%; Lys, 88.7%; Met, 87.8%; Phe, 88.4%; Thr, 81.4%; Trp, 73.6%; Val, 84.5%. OA supplementation decreased the AID and SID (P < 0.05) of Arg, Ile, Asp, and Glu, and tended (P < 0.10) to decrease the AID and SID of Phe, Trp, Cys, Gly, and Tyr. MC supplementation did not affect the AID and SID of AA, and no interactive effect on AA digestibility was observed between OA and MC supplementation. In conclusion, dietary supplementation of OA and MC showed no positive effects on improving AA digestibility in DESBM fed to growing pigs.

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PSI-12 Effect of a carbohydrase mixture on apparent and standardized ileal digestibility of amino acids in dry extruded-expelled soybean meal fed to growing pigs

Journal of Animal Science ◽

10.1093/jas/skz258.592 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 292-293

Author(s):

Jichen Song ◽

Ahmed Aljuobori ◽

C Martin Nyachoti ◽

Gustavo A Mejicanos

Keyword(s):

Soybean Meal ◽

Mixed Model ◽

Latin Square ◽

Experimental Period ◽

Sole Source ◽

Antinutritional Factors ◽

Nutrient Digestibility ◽

Growing Pigs ◽

Mean Values ◽

Ileal Digestibility

Abstract Heat treatment could effectively decrease the antinutritional factors in soybean during meal processing. One such progress is the combination of extrusion with expelling, which produces the dry extruded-expelled soybean meal (DESBM), and there is a great interest in exploring its utilization as a dietary ingredient for swine. Thus, eight ileal-cannulated barrows (initial BW = 30 ± 1.3 kg) were used to determine the ileal amino acid (AA) digestibility of DESBM fed to growing pigs with or without multi-carbohydrase (MC) supplementation. Pigs were randomly assigned to 1 of 4 treatments in a replicated 4 × 4 Latin square design to give eight observations per treatment. The experimental diets were formulated to contain one of the two batches of DESBM (DESBM-1 and DESBM-2) as the sole source of protein. All diets contained titanium dioxide (0.3%) as an indigestible marker to calculate nutrient digestibility. Each experimental period lasted for seven days, and ileal digesta samples were collected on d 6 and d 7. The standardized ileal digestibility (SID) of AA were calculated using published mean values to estimate ileal endogenous AA losses. Data were analyzed using the mixed model procedures of SAS, and the final model had treatment, batch, and the interaction between treatment and batch as the main effects. In general, the SID of AA in DESBM-1 and DESBM-2 were not different, such as Ile, Lys, Met, Thr, and Val, which averaged 88.97%, 89.77%, 89.08%, 84.38%, and 86.85% respectively. There were no effects of MC supplementation on AID and SID of AA digestibility in DESBM except for the AID of Trp (P < 0.05). Also, there were no interaction effects of MC supplementation and batch on AA digestibility in DESBM. In conclusion, enzyme supplementation did not improve the AID and SID of AA in DESBM fed to growing pigs.

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PSVI-13 Amino Acid Supplementation During the Adaptation Period Did Not Affect the Standardized Ileal Digestibility of Amino Acids in Corn and Soybean Meal Fed to Pigs

Journal of Animal Science ◽

10.1093/jas/skab054.327 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 200-201

Author(s):

Hyunjun Choi ◽

Sun Jong You ◽

Beob Gyun G Kim

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Soybean Meal ◽

Latin Square ◽

Sole Source ◽

Adaptation Period ◽

Amino Acid Supplementation ◽

Ileal Digestibility ◽

Indispensable Amino Acids ◽

Dietary Treatments

Abstract The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P < 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P < 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.

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Effect of a Multi-Carbohydrase and Phytase Complex on the Ileal and Total Tract Digestibility of Nutrients in Cannulated Growing Pigs

Animals ◽

10.3390/ani10081434 ◽

2020 ◽

Vol 10 (8) ◽

pp. 1434 ◽

Cited By ~ 1

Author(s):

Jia-Cheng Yang ◽

Li Wang ◽

Ya-Kuan Huang ◽

Lei Zhang ◽

Rui Ma ◽

...

Keyword(s):

Organic Matter ◽

Soybean Meal ◽

Crude Protein ◽

Latin Square ◽

Dietary Supplementation ◽

Growing Pigs ◽

Distal Ileum ◽

Ileal Digestibility ◽

Gross Energy ◽

Corn Distillers

The current study evaluated the influence of a multi-carbohydrase and phytase complex (MCPC) on the ileal and total tract digestibility of nutrients in growing pigs. A total of eight barrows (initial BW = 30.7 ± 1.1 kg) were surgically fitted with a T-cannula at the distal ileum and randomly allotted to four groups. The experiment was conducted according to a 4 × 4 Latin square design, each period lasting 10 days. Pigs were fed four experimental diets, which consisted of two basal diets (BD1, low phytate; BD2, high phytate) with or without MCPC containing at least 1800 U xylanase, 6600 U α-arabinofuranosidase, 1244 U β-glucanase, and 1000 U phytase per/kg corn–soybean meal with 15% corn distillers based diet. The high phytate diet reduced (p < 0.05) the apparent ileal digestibility (AID) of crude protein by 1.4% and the apparent total tract digestibility (ATTD) of organic matter, crude protein, and gross energy by 1.7, 2.3, and 1.9%, respectively, and tended to decrease (p = 0.10) the ATTD of Ca by 17.3%, relative to the low phytate diet. The dietary supplementation of the MCPC increased (p < 0.05) the AID of phosphorus (P) and calcium (Ca) by 34.2% and 31.1% for BD1 and 26.7% and 41.3% for BD2, respectively, and increased (p < 0.05) ATTD of crude fat, P, and Ca by 1.4%, 45.6%, and 9.6% for BD1 and 3.1%, 66.0%, and 52.7% for BD2, respectively. The MCPC supplementation did not significantly increase the AID and (or) ATTD of crude protein, organic matter, and starch. In conclusion, the dietary supplementation of the MCPC could improve the AID of P and Ca and the ATTD of crude fat, P, and Ca.

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PSVI-18 Nutritional Value of High-protein Yeast Products Fed to Growing Pigs in Comparison to Commonly Used Protein Sources in Swine Diets

Journal of Animal Science ◽

10.1093/jas/skab054.333 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 204-204

Author(s):

Ricardo Garavito Duarte ◽

Jorge Y Perez-Palencia ◽

Kevin Herrick ◽

Crystal L Levesque

Keyword(s):

Soybean Meal ◽

Latin Square ◽

Sole Source ◽

Growing Pigs ◽

High Protein ◽

Ileal Digestibility ◽

Protein Sources ◽

Alternative Ingredients ◽

Diet Formulation ◽

Swine Diets

Abstract Alternative ingredients and co-products from the ethanol and biofuel industries represent an opportunity for utilization in swine diets. The objective of this study was to determine standardized ileal digestibility (SID) of AA in two yeast-based high protein DDGS products (HP-DDGS) fed to growing pigs in comparison to common swine diet protein sources. Cornstarch-based diets were formulated using six ingredients as the sole source of AA: spray dried HP-GDDS, ring dried HP-GDDS, conventional DDGS, soybean meal (SBM), enzymatically-treated soybean meal (HP300) and fishmeal (FM). A N-free diet was used to estimate endogenous ileal AA losses. Seven barrows (25 ± 0.8 kg BW) were fitted with a simple T-cannula at the distal ileum. The experiment was conducted as a 7 × 7 Latin square design with 7 collection periods of 7d (5d adaptation and 2d ileal digesta collection). Pigs were fed at 4% of BW. Diets and ileal digesta were analyzed for AA and titanium. Digestibility values were calculated according to the marker method. The CP and AA concentrations in Spray HP-GDDS and Ring HP-GDDS samples were higher than in the DDGS sample and similar to the SBM and HP300 samples. General SID values were within the mean +/- SD of NRC (2012) values for the six ingredients evaluated. The SID of AA was greater (P < 0.05) in HP300 than in the other protein sources (90.09 vs. 79.97). The SID values were generally similar (P > 0.05) in SBM, FM, Spray HP-GDDS, and Ring HP-GDDS samples (81.49, 78.71, 81.52, and 79.20). The SID values for Lys were greater (P < 0.05) in Spray HP-GDDS than in Ring HP-GDDS and DDGS (83.56 vs. 77.33 and 68.53). The HP-GDDS products are potential alternative ingredients to commonly used protein sources in swine diets and the obtained data allows its use in practical diet formulation.

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PSIII-17 Extrusion Enhances Nutrient and Energy Digestibility of Pulse Grain-based Diets Fed to Growing Pigs

Journal of Animal Science ◽

10.1093/jas/skab054.298 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 176-177

Author(s):

Joaquin J Sanchez Zannatta ◽

L F Wang ◽

Eduardo Beltranena ◽

Ruurd T Zijlstra

Keyword(s):

Soybean Meal ◽

Fish Meal ◽

Latin Square ◽

Nutrient Digestibility ◽

Growing Pigs ◽

Animal Protein ◽

Protein Source ◽

Heat Processing ◽

Ileal Digestibility ◽

Single Screw Extruder

Abstract Heat processing may enhance nutrient digestibility of diets based on pulse grains. The objective of this study was to determine nutrient and energy digestibility of extruded lentil-based diets containing either supplemental plant or animal protein source in growing pigs. Two diets were formulated to provide 2.4 Mcal NE/kg and 4.35 g standardized ileal digestible Lys/Mcal NE: 1) soybean meal diet (SBM), containing 50% lentil, 31% wheat, and 12.8% soybean meal; and 2) fish meal diet (FM), containing 40% lentil, 45% wheat, and 10% fish meal. Following mixing, each diet was divided into 2 parts: one part remained mash while the other part was extruded using a single-screw extruder (105°C, 400 rpm, 5 kg water/h). The 2 extruded and 2 non-extruded diets were tested in a 2 × 2 factorial arrangement. Eight ileal-cannulated barrows (32.3 kg) were fed the 4 diets at 2.8 times maintenance DE (110 kcal per kg of BW0.75) for four 9-d periods in a double 4 × 4 Latin square. The FM diets had greater (P < 0.05) AID of DM, GE, and most AA, and ATTD of CP, but lower apparent hindgut fermentation of DM and GE than the SBM diets. The AID of CP and AA were 3.2 and 4.7%-units greater (P < 0.05), respectively, and the ATTD of GE and DE values were 2.1 and 3.1%-units greater (P < 0.05), respectively, for the extruded diets than the non-extruded diets. Interactions between protein source and extrusion were not observed. In conclusion, FM diets had greater ileal digestibility of DM, energy, and AA than SBM diets. Extrusion increased the AID of CP and most AA, and DE value of both plant- and animal-protein diets based on lentil grain, indicating that extrusion can increase the energy and protein value of pulse-grain based diets fed to growing pigs.

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PSVII-2 Influence of source and concentration of phosphorus on apparent ileal digestibility and apparent total tract digestibility of phosphorus in growing pigs

Journal of Animal Science ◽

10.1093/jas/skz258.719 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 360-360

Author(s):

Ayodeji Aderibigbe ◽

Olayiwola Adeola

Keyword(s):

Regression Analysis ◽

Soybean Meal ◽

Latin Square ◽

Growing Pigs ◽

Ileal Digestibility ◽

P Loss ◽

Corn Distillers ◽

Fecal Collection

Abstract A study was conducted to examine the influence of source and concentration of phosphorus (P) on the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of P in growing pigs. Six diets were formulated in a 2 × 3 factorial arrangement with soybean meal (SBM) or corn distillers’ dried grain with solubles (DDGS) as sole sources of dietary P, and concentration of P at 2.0, 2.5 or 3.0 g/kg. Eighteen cannulated pigs (initial BW = 40 ± 5 kg) were allotted to triplicate 6 × 3 incomplete Latin square design with 6 diets and 3 periods, which consisted of 5 d adaption, 2 d fecal collection, and 2 d ileal digesta collection. The results indicated that AID of P was not different from ATTD of P in all diets (P = 0.45). The digestibility of P was affected by source of P (P < 0.01), with mean of 33.7% in SBM and 50.3% in DDGS. The AID of P in SBM increased linearly (P < 0.05) with increasing P concentrations; however, there was no effect of increasing P concentrations on AID and ATTD of P in DDGS. Determined by regression analysis, true ileal digestibility of P was 58.3% or 57.6%, and true total tract digestibility of P was 56.0% or 62.6%, for SBM or DDGS respectively. The estimated ileal endogenous P loss was 0.61 or 0.13 g//kg DMI, and total tract endogenous P loss was 0.53 or 0.35 g/kg DMI, for SBM or DDGS respectively. In summary, source and concentration of dietary P affect the digestibility of P in growing pigs but there was no difference between AID and ATTD of P.

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Effects of distillers dried grains with solubles on amino acid digestibility, growth performance, and carcass characteristics of growing pigs1

Translational Animal Science ◽

10.1093/tas/txz005 ◽

2019 ◽

Vol 3 (2) ◽

pp. 641-653

Author(s):

Shelby M Curry ◽

Laia Blavi ◽

Julian Wiseman ◽

Hans H Stein

Keyword(s):

Growth Performance ◽

Latin Square ◽

Sole Source ◽

Growing Pigs ◽

Carcass Quality ◽

Finishing Pigs ◽

Ileal Digestibility ◽

Distillers Dried Grains ◽

Feeding Program ◽

Dietary Treatments

Abstract Two experiments were conducted to compare the standardized ileal digestibility (SID) of AA by growing pigs in European distillers dried grains with solubles (DDGS) produced from wheat, maize, or wheat–maize mixtures and to test the effect of increasing the inclusion levels of wheat DDGS on growth performance of growing-finishing pigs fed diets balanced for NE and SID Lys. In Exp. 1, 12 barrows (initial BW: 23.0 ± 2.2 kg) were surgically equipped with a T-cannula in the distal ileum and randomly allotted to a replicated 6 × 6 Latin square design with six diets and six periods. Five sources of European DDGS were used: wheat DDGS from 2011, wheat DDGS from 2012, wheat-80 DDGS (80% wheat and 20% maize), wheat-70 DDGS (70% wheat and 30% maize), and maize DDGS. Each diet contained one source of DDGS as the sole source of AA and an N-free diet was used to determine basal endogenous losses of AA. Results indicated that the SID of CP was greater (P < 0.05) in maize DDGS compared with wheat DDGS from 2011, wheat DDGS from 2012, and wheat-70 DDGS. The SID of all indispensable AA except Trp was also greater (P < 0.05) in maize DDGS compared with all other DDGS sources used in this experiment. For Trp, the SID in wheat-80 DDGS, wheat DDGS from 2011, and wheat DDGS from 2012 were not different from maize DDGS, but were greater (P < 0.05) than in wheat-70 DDGS. The SID for all indispensable AA except Ile and Trp in wheat-70 DDGS were not different from the values calculated for wheat DDGS from 2011 and wheat DDGS from 2012, and no differences between SID values for AA in wheat DDGS from 2011 and wheat DDGS from 2012 were observed. In Exp. 2, 36 growing pigs (initial BW: 38.3 ± 1.97 kg) were randomly allotted to one of four dietary treatments (one pig/pen and nine replicate pigs/treatment) in a 2-phase feeding program (35 to 65, and 35 to 105 kg BW). The four dietary treatments included diets containing 0%, 10%, 20%, or 30% wheat DDGS. Results indicated that there was no effect of wheat DDGS on pig growth performance or carcass quality. However, addition of wheat DDGS increased linearly (P < 0.015) the indole concentration in the carcasses of the pigs. In conclusion, the SID of AA in maize DDGS produced in Europe is greater than in European wheat DDGS and DDGS produced from mixtures of wheat and maize, but inclusion of 30% wheat DDGS in diets fed to growing-finishing pigs did not affect growth performance or carcass quality.

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199 Effect of the Pelleting Process on Diet Formulations with Varying Levels of Crystalline Amino Acids and Reducing Sugars on Digestibility in Growing Pigs

Journal of Animal Science ◽

10.1093/jas/skab054.110 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 66-67

Author(s):

Kara M Dunmire ◽

Diego A Lopez ◽

Chance J Fiehler ◽

Yiqin Zhang ◽

Cassandra K Jones ◽

...

Keyword(s):

Amino Acids ◽

Reducing Sugars ◽

Latin Square ◽

Growing Pigs ◽

Ileal Digestibility ◽

Distillers Grains With Solubles ◽

Feed Form ◽

Low Crystalline ◽

Crystalline Amino Acids ◽

Main Effects

Abstract The objective of this study was to determine effects of pelleting on the standardized ileal digestibility (SID) of amino acids (AA) in diets with or without increased concentrations of free AA and reducing sugars (RS). Eight individually housed, ileal cannulated barrows (initially 69.2 kg) were allotted to a replicated 8×8 Latin square with 8 diets and eight 7-d periods with ileal digesta collected on d 6 and 7. Treatments were arranged in a 2×2×2 factorial with main effects of diet form (mash vs. pellet), crystalline AA (low vs. high), or reducing sugars (low vs. high) provided by dried distillers grains with solubles and bakery meal. Diets were pelleted to achieve a hot pellet temperature of 85 to 88°C. Data were analyzed as a completely randomized Latin square using the GLIMMIX procedure of SAS. A feed form×RS interaction (P < 0.026) for SID of tryptophan was observed. Feeding pelleted low RS diets improved SID of tryptophan compared with mash high and low RS diets, and pelleted high RS diets. For main effects of feed form, the SID of total AA, CP, and indispensable AA increased (P < 0.042) in pigs fed pelleted diets compared with mash diets. For main effects of crystalline AA, pigs fed high crystalline AA had increased (P = 0.007) SID of tryptophan and decreased (P = 0.050) SID of histidine compared with those fed low crystalline AA diets. For main effects of RS diets, pigs fed high RS diets had decreased (P < 0.05) SID of total AA, CP and indispensable AA. In conclusion, pelleting diets with increased crystalline AA or RS did not affect the improvement in AA digestibility from pelleting. Pelleting diets improved AA digestibility. Diets formulated with high crystalline AA had increased SID of tryptophan. Formulating diets with high RS resulted in decreased AA digestibility compared with corn-soybean meal-based diets.

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Effect of fermentation of soybean meal with varying protein solubility on ileal digestibility of nutrients in growing pigs

Animal Production Science ◽

10.1071/an14842 ◽

2016 ◽

Vol 56 (12) ◽

pp. 2023

Author(s):

Santi D. Upadhaya ◽

Je-hoon Ryu ◽

Kyung-il Kang ◽

Seong-Jun Cho ◽

In Ho Kim

Keyword(s):

Amino Acids ◽

Soybean Meal ◽

Crude Protein ◽

Dry Matter ◽

Protein Solubility ◽

Latin Square ◽

Growing Pigs ◽

Ileal Digestibility ◽

Fermented Soybean Meal ◽

Fermented Soybean

Apparent ileal digestibility and standardised ileal digestibility of energy, dry matter, nitrogen and amino acids were evaluated in five cannulated [(Landrace × Yorkshire) × Duroc)] barrows with an initial bodyweight of 27.1 ± 0.58 kg in a 5 × 5 Latin square design. Pigs were fed unfermented soybean meal (USM) as control and fermented soybean meal A, B and C (FSMA, FSMB and FSMC, respectively), with 52.4%, 62.0% and 71.8% protein solubility, respectively, and a nitrogen-free diet. The nitrogen-free diet was used to determine basal endogenous losses of crude protein and amino acids. The apparent ileal digestibility of dry matter and nitrogen was greater (P < 0.05) in FMSC and tended to improve in FSMA and FSMB compared with the control. The apparent ileal digestibility of isoleucine, phenylalanine and valine was greater in all fermented soybean meal whereas that of lysine was greater (P < 0.05) in FSMC compared with USM. Likewise, the apparent ileal digestibility of aspartic acid and proline was greater (P < 0.05) in FSMA and FSMC. The standardised ileal digestibility of crude protein and lysine was higher (P < 0.05) in FSMC and tended to improve in FSMA and FSMB whereas the standardised ileal digestibility of isoleucine was higher (P < 0.05) in FSMA, FSMB and FSMC compared with USM. In conclusion, fermentation of soybean meal showed better digestibility compared with USM regardless of its protein solubility values.

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136 Nutritive value of phytase- and multi-carbohydrase-supplemented carinata meal for growing pigs

Journal of Animal Science ◽

10.1093/jas/skz258.240 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 116-117

Author(s):

Jinsu Hong ◽

Jung Wook Lee ◽

Dan Pettersson ◽

Tofuko A Woyengo

Keyword(s):

Soybean Meal ◽

Nutritive Value ◽

Latin Square ◽

Basal Diet ◽

Growing Pigs ◽

Net Energy ◽

Initial Body Weight ◽

Ileal Digestibility ◽

Gross Energy ◽

The Difference

Abstract Objective of the study was to determine the effect of supplementing a combination of phytase and multi-carbohydrase on standardized ileal digestibility (SID) of amino acid (AA) and net energy (NE) value of carinata meal for growing pigs. Ten ileal-cannulated barrows (initial body weight = 53.9 ± 4.76 kg) were fed 4 diets in a replicated 4 × 4 Latin square design with 2 additional columns to give 10 replicates per diet. Diets included a corn-soybean meal (SBM)-based basal diet, the basal diet with 25% carinata meal, basal diet with 25% carinata meal plus phytase at 2,000 FTU/kg and multi-carbohydrase at 0.2g/kg, and a nitrogen-free diet. The multi-carbohydrase supplied 4 units of xylanase, 10 units of β-glucanase, and 1000 units of pectinase per kilogram of diet. The ratio of corn to soybean meal and soybean oil in carinata meal- containing diets was identical to that in the corn-soybean meal based basal diet to allow calculation of AA and energy digestibility of carinata meal by the difference method. On a DM basis, carinata meal contained 50.2% CP, 0.88% ether extract, 15.37% ADF, 1.82% Lys, 0.96% Met, 1.89% Thr, and 0.64% Trp, respectively. The SID of Lys, Met, Thr, and Trp for carinata meal were 51.4%, 82.2%, 65.9%, and 85.9%, respectively. Supplementation of a combination of phytase and multi-carbohydrase did not affect the SID of AA for the corn-SBM-carinata meal-based diet, and for the carinata meal. However, supplementation of a combination of phytase and multi-carbohydrase improved (P = 0.015) the apparent total tract digestibility of gross energy, and DE and NE values for carinata meal by 9.4, 9.5, and 12.4% respectively. In conclusion, the enzymes used in the current study could be added in carinata meal-based diets for pigs to improve the energy value of the carinata meal.

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