PSV-4 Determination of Deoxynivalenol (DON) Content in Biological Samples as an Indicator of DON Intake in Grower-finisher Pigs

Due to difficulties in obtaining consistent and/or reliable measures of deoxynivalenol (DON) in complete swine diets, we investigated whether measuring DON in biological samples could be used as an indicator of DON ingestion in pigs. In this study, graded levels of DON (1, 3, or 5 ppm) were fed to grower-finisher pigs for a period of 77-d. On d 35 and 77 of the study, urine samples were quantitatively collected over a 24-h period and blood samples were collected between 3 – 4 h after the morning meal on each of those days for serum DON analysis. For direct quantification of DON in urine, high-performance liquid chromatography with tandem mass spectrometry was performed. For serum samples, indirect quantification of DON was performed via enzymatic hydrolysis. We observed that DON content in urine increased linearly as intake of DON increased (Fig.1A; P < 0.05). Analysis of DON in serum follow a similar trend, where serum DON content was increased as DON intake increased (Fig.1B; P < 0.05). An average of 30% of DON ingested was recovered as DON in urine over a 24-h period. In summary, there was a linear relationship between DON intake and DON content in both urine and blood serum, therefore, analyzing DON concentration in serum and urine could be used as a tool to estimate for DON exposure in pigs under controlled conditions.

PSVI-18 Nutritional Value of High-protein Yeast Products Fed to Growing Pigs in Comparison to Commonly Used Protein Sources in Swine Diets

Alternative ingredients and co-products from the ethanol and biofuel industries represent an opportunity for utilization in swine diets. The objective of this study was to determine standardized ileal digestibility (SID) of AA in two yeast-based high protein DDGS products (HP-DDGS) fed to growing pigs in comparison to common swine diet protein sources. Cornstarch-based diets were formulated using six ingredients as the sole source of AA: spray dried HP-GDDS, ring dried HP-GDDS, conventional DDGS, soybean meal (SBM), enzymatically-treated soybean meal (HP300) and fishmeal (FM). A N-free diet was used to estimate endogenous ileal AA losses. Seven barrows (25 ± 0.8 kg BW) were fitted with a simple T-cannula at the distal ileum. The experiment was conducted as a 7 × 7 Latin square design with 7 collection periods of 7d (5d adaptation and 2d ileal digesta collection). Pigs were fed at 4% of BW. Diets and ileal digesta were analyzed for AA and titanium. Digestibility values were calculated according to the marker method. The CP and AA concentrations in Spray HP-GDDS and Ring HP-GDDS samples were higher than in the DDGS sample and similar to the SBM and HP300 samples. General SID values were within the mean +/- SD of NRC (2012) values for the six ingredients evaluated. The SID of AA was greater (P < 0.05) in HP300 than in the other protein sources (90.09 vs. 79.97). The SID values were generally similar (P > 0.05) in SBM, FM, Spray HP-GDDS, and Ring HP-GDDS samples (81.49, 78.71, 81.52, and 79.20). The SID values for Lys were greater (P < 0.05) in Spray HP-GDDS than in Ring HP-GDDS and DDGS (83.56 vs. 77.33 and 68.53). The HP-GDDS products are potential alternative ingredients to commonly used protein sources in swine diets and the obtained data allows its use in practical diet formulation.

PSIV-20 Impact of High Protein Dried Distiller Grains and Soybean Meal Inclusion Level on Grow-finish Pig Performance and Carcass Traits

High protein dried distiller grains are a novel protein source for commercial swine diets. Questions related to how concentrations of branched chain amino acids (BCAA) in corn protein sources affect pig performance remain. A randomized complete block design (RCBD) grow-finish experiment was conducted at the South Dakota State University Commercial Research Facility using NexPro® protein ingredient (HPDDG) to determine the performance response of grow-finish pigs fed corrected levels of BCAA through soybean meal (SBM) or synthetic amino acids (SAA). Pigs (n = 1,170; 59.5 ± 0.48 kg) were used in a 79-d study (9 replicates per treatment). Pens of pigs were allotted to one of five dietary treatments which consisted of 1) corn-SBM diet, 2) HPDDG not corrected for BCAA levels or HPDDG corrected for BCAA levels through additions of 3) SBM, 4) 50% SBM and 50% SAA blend or 5) SAA only. The HPDDG were included in diets at 15% in phase 1 and 10% in phases 2 and 3. Additions of SAA were included to maintain a SID Ile:Lys of 65% and SID Val:Lys of 75% for BCAA corrected diets. Data was analyzed as a RCBD, pair-wise comparisons and single degree of freedom orthogonal polynomials (BCAA corrected only) were used to evaluate treatment responses, and pen served as the experimental unit for all analyses. Reducing SBM in BCAA corrected diets decreased cumulative ADG and G:F (linear; P < 0.05). Decreasing SBM in BCAA corrected diets tended to decrease (linear; P = 0.09) standardized fat-free lean but increase (linear; P = 0.07) dressing percent and back fat depth (quadratic; P = 0.08) while not impacting hot carcass weight (P > 0.14). This data indicates HPDDG is a suitable feedstuff for grow-finish swine diets at low inclusion levels due to minimal impact on performance and carcass characteristics. Correction of BCAA levels through SBM inclusion provided an improved performance response compared to SAA only.

Phenolic compounds as natural feed additives in poultry and swine diets: a review

Due to ban on using antibiotics in feed industry, awareness of using natural feed additives have led to a great demand. The interest of plants phenolic compounds as a potential natural antioxidant source has been considered in research community due to their predictable potential role as feed additives in poultry and swine production. However, the mode of action for their functional role and dosage recommendation in animal diets are still remain indistinct. Taking into account, the present review study highlights an outline about the mode of action of phenolic compound and their experimental uses in poultry and swine focusing on the growth performance, antioxidant function, immune function, antimicrobial role and overall health status, justified with the past findings till to date. Finally, the present review study concluded that supplementation of phenolic compounds as natural feed additives may have a role on the antioxidant, immunity, antimicrobial and overall production performance in poultry and swine.

Effects of Sources or Formulations of Vitamin K3 on Its Stability during Extrusion or Pelleting in Swine Feed

Two studies were conducted to determine the stability of vitamin K3 (VK3) in swine diets during extrusion or pelleting. The two sources were menadione sodium bisulfite (MSB) and menadione nicotinamide bisulfite (MNB), and the three formulations were crystal micro-capsule formulation and micro-sphere formulation. The recovery of six types of VK3 in swine diets was investigated after extrusion at 100 °C or 135 °C in Experiment 1. The recovery of six types of VK3 was investigated when the diets were pelleted at 60 °C (low temperature; LT) or 80 °C (high temperature; HT) and the length to diameter ratios were 5.2:1 (low length to diameter ratio; LR) or 7.2:1 (high length to diameter ratio; HR) in Experiment 2. In Experiment 1, MNB recovery (72.74%) was higher than MSB recovery (64.67%) after extrusion, while recovery of VK3 of crystal (74.16%) was higher than the recovery of micro-capsule (65.25%) and micro-sphere (66.72%). The recovery of VK3 (70.88%) was higher when extruded at 100 °C than that at 135 °C (66.54%). In Experiment 2, MNB recovery (86.21%) was higher than MSB recovery (75.49%) after pelleting, while the recovery of VK3 of micro-capsule (85.06%) was higher than the recovery of crystal (81.40%) and micro-sphere (76.09%). The recovery of VK3 (75.50%) was lower after HTHR pelleting than LTLR (83.62%), LTHR (81.52%) or HTLR (82.76%) treatment. Our results show that MNB has greater stability than MSB. VK3 of crystal or VK3 of micro-capsule were recommended for extrusion or pelleting, respectively.

PERFORMANCE AND CARCASS CHARACTERISTICS OF FINISHING PIGS FED RAW OR EXTRUDED FULL-FAT SOYA BEANS RATIONS IN HUMID LOWLAND TROPICS

Sixteen Landracex Large White crossbred pigs averaging 56 kg body weight were used in a study to uvestigate the effects of feeding raw full-fat soyu bean (RSB) rations on the performance of pigs in humid lowland tropics. Raw full-lat soya bean was used to replace extruded full-fat soyabean at 33, 67 and 100% in finishing swine diets having 16% C.P. and 2800 kcal/kgME and fed ad lib. ADG was reduced by the addition of RSB to the diets (P>0.05). However, FE was improved (P>0.05) when 67 over (P>0.05) when 67 and 100% RSB were added to the diets. RSB significantly (P<0.05) affected backfat deposition and percent lean cut of the carcass. Other carcass traits were not affected.