scholarly journals PSVIII-34 Late-Breaking Abstract: Additivity of digestible amino acids in hatchery byproducts fed to nursery pigs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 343-345
Author(s):  
Beob G Kim ◽  
Jung Yeol Sung

Abstract The objectives were to determine standardized ileal digestibility (SID) of amino acids (AA) in hatchery byproducts fed to nursery pigs and to test the additivity of ileal digestible AA concentrations in hatchery byproducts. Ten barrows (initial body weight = 11.3 ± 1.3 kg) surgically fitted with T-cannulas at the distal ileum were individually housed in pens. Four diets were prepared to contain 30% of infertile eggs, unhatched eggs, culled chicks, or a mixture (20% dried infertile eggs + 20% dried unhatched eggs + 60% dried culled chicks) as the sole source of nitrogen. A nitrogen-free diet was additionally prepared to measure basal endogenous losses of AA. All experimental diet contained 0.5% chromic oxide as an indigestible index. Ten pigs were assigned to a replicated 5 × 4 Latin square design with 5 diets and 4 periods. Each period consisted of a 5-d adaptation period and a 2-d collection period. The hypothesis was that the determined digestible AA concentrations of the mixture are not different from the calculated values based on digestible AA in the individual ingredients. A t-test was used to determine the additivity of digestible AA concentrations in individual ingredients. Infertile eggs had greater (P < 0.05) SID of all indispensable AA except for lysine and methionine compared with culled chicks. The SID of all indispensable AA except for tryptophan was not different between culled chicks and the mixture. The calculated apparent and standardized ileal digestible AA in the mixture were not different from the determined values, but calculated ileal digestible tryptophan in the mixture was less (P < 0.05) than the measured value. In conclusion, ileal digestibility of most indispensable AA in infertile eggs is greater than that in culled chicks and ileal digestible AA values are additive in the mixture except for tryptophan.

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 200-201
Author(s):  
Hyunjun Choi ◽  
Sun Jong You ◽  
Beob Gyun G Kim

Abstract The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P < 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P < 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.


2020 ◽  
Vol 98 (11) ◽  
Author(s):  
Maryane S F Oliveira ◽  
Jerubella J Abelilla ◽  
Neil W Jaworski ◽  
John K Htoo ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that addition of crystalline amino acids (AA) to diets during the adaptation or collection periods will not influence calculated values for apparent ileal digestibility (AID) or standardized ileal digestibility (SID) of AA in corn and soybean meal (SBM). Seven ileal-cannulated barrows (initial body weight: 77.9 ± 2.6 kg) were allotted to a 7 × 7 Latin Square design with 7 dietary treatments and 7 periods. Dietary treatments included feeding diets based on corn or SBM without or with crystalline AA for the entire 7-d period or with crystalline AA during the adaptation period, but without crystalline AA during the collection period. An N-free diet was also used. Each experimental period consisted of 5 d of adaptation to the diets followed by 2 d of ileal digesta collection, with digesta being collected for 9 hr/d starting after feeding the morning meal. Thus, the entire experiment lasted 49 d. The AID and SID of crude protein (CP) and AA were calculated using values determined in corn or in SBM without or with crystalline AA to determine if crystalline AA influenced calculated values for AID or SID of CP and AA. Results indicated that addition of crystalline AA to diets fed during the entire 7-d period increased (P < 0.05) AID of some AA in corn and SBM. However, no differences in SID of CP and AA were observed between pigs fed the diets without crystalline AA and pigs fed the diets with crystalline AA if dietary crystalline AA were ignored in calculations of SID values. This indicates that crystalline AA were 100% absorbed before the distal ileum and did not affect calculated values for SID of AA if calculations were based only on the AA in corn or SBM. Therefore, it is concluded that crystalline AA may be added to experimental diets in digestibility experiments before and during collection periods without affecting results, if crystalline AA are disregarded in the calculation of AID or SID of AA in ingredients.


2020 ◽  
Vol 98 (1) ◽  
Author(s):  
L Vanessa Lagos ◽  
Hans H Stein

Abstract Three experiments were conducted to test the hypothesis that the standardized ileal digestibility (SID) of AA, concentrations of DE and ME, and the standardized total tract digestibility (STTD) of P in a source of torula yeast are not different from values obtained in Menhaden fish meal. In experiment 1, six weanling barrows (initial BW: 11.7 ± 0.4 kg) were prepared with a T-cannula in the distal ileum and allotted to a replicated 3 × 3 Latin square design with 3 diets and 3 periods. In each period, there were 5 d of adaptation and 2 d of collection. Two cornstarch-based diets using the torula yeast or fish meal as the sole source of AA and a N-free diet were formulated. The SID of CP and all AA was greater (P < 0.05) in torula yeast than in fish meal. In experiment 2, 24 weanling barrows (initial BW: 14.4 ± 1.1 kg) were individually housed in metabolism crates and allotted to a corn-based diet or 2 diets based on a mixture of corn and torula yeast or corn and fish meal. Feces and urine samples were collected for 4 d following a 5-d adaptation period. There were 8 replicate pigs per diet and fecal and urine materials were collected. Results of this experiment indicated that there were no differences in the concentration of DE and ME (DM basis) between torula yeast and fish meal. In experiment 3, a total of 32 weanling barrows (initial BW: 11.9 ± 1.1 kg) were allotted to 4 diets and 8 replicate pigs per diet. Pigs were placed in individual metabolism crates. The torula yeast or fish meal were used in 2 diets containing either 0 or 500 units of microbial phytase. Feces samples were collected as described for experiment 2. The STTD of P in torula yeast was greater (P < 0.05) than in fish meal, but regardless of ingredient, there was no effect of the inclusion of phytase in the diets. In conclusion, the SID of AA and the STTD of P in torula yeast is greater than in fish meal, but values for the concentration of DE and ME in torula yeast are not different from those in fish meal. Therefore, the torula yeast that was used in the present experiments may be included at the expense of fish meal in diets fed to weanling pigs if the concentration of standardized ileal digestible AA is considered in the formulation.


Author(s):  
Nan Zhang ◽  
Xiaoming Song ◽  
Wenxuan Dong ◽  
Ling Liu ◽  
Zhiying Cui ◽  
...  

Abstract This study was conducted to determine the digestible energy (DE), metabolizable energy (ME), and ileal digestibility of amino acids (AA) of fish protein hydrolysate (FPH), and to evaluate the effects of FPH on the performance of piglets. In Exp. 1, the available energy content of FPH was determined by difference methods: 12 barrows with an initial body weight (BW) of 40.0±2.1kg were randomly assigned into two dietary treatments with six replicates per treatment, the treatments contained a corn basal diet and an experimental diet in which 20% corn was replaced by FPH. The experiment was lasted for 12 days, with 7 days adaptation period followed by 5 days total collection of feces and urine. In Exp. 2, the standardized ileal digestibility (SID) of AA in FPH by the N-free method was determined, 12 barrows (initial BW of 35.3±1.8 kg) with ileal T-cannulas were randomly allotted into two treatments with six replicates per treatment, the dietary treatments consisted of an experimental diet and a N-free diet. The experiment was lasted for 7 days, with 5 days adaptation period followed by 2 days collection of ileal digesta. In Exp. 3, the effect of FPH on the performance of piglets was conducted. 192 piglets (initial BW of 8.10±1.8kg) were randomly allotted into four treatments with six replicates per treatment. The treatments were a control diet with fish meal (FM) and part or all FM was replaced by FPH, got diets containing 2%, 3% or 5% FPH. The experiment lasted for 28 days. Results showed that: In Exp. 1, the DE and ME of FPH were 21.12MJ/kg and 20.28MJ/kg. In Exp. 2, the SID of Lys, Met, Thr and Trp were 79.99%, 87.17%, 68.29%, and 71.53% respectively. In Exp. 3, addition of 3% FPH increased nutrient digestibility and volatile fatty acid content in feces. Addition of 5% FPH increased the average daily feed intake (ADFI), significantly increased (P<0.05) the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and immunoglobulin A (IgA), while significantly decreased (P<0.05) the average daily gain (ADG). In conclusion, FPH had a high value of digestible energy and ileal digestible essential amino acids, could improve nutrient digestibility, immunity, antioxidant capacity, and intestinal health of piglets, while adverse to nitrogen deposition in piglets.


2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Minoy Cristobal ◽  
Jessica P Acosta ◽  
Su A Lee ◽  
Hans H Stein

Abstract AbstractThree experiments were conducted to test the hypothesis that standardized ileal digestibility (SID) of amino acids (AA), concentration of metabolizable (ME), and standardized total tract digestibility (STTD) of P in a new source of distillers dried grains with solubles (DDGS; ProCap DDGS) are greater than in conventional de-oiled DDGS. In experiment 1, nine barrows (initial BW: 67.2 ± 6.4 kg) with a T-cannula in the distal ileum were allotted to a triplicated 3 × 3 Latin square design with three diets and three periods for a total of nine replicate pigs per diet. Two diets included ProCap DDGS or de-oiled DDGS as the sole source of crude protein (CP) and AA. An N-free diet was used to determine the basal endogenous losses of CP and AA. Ileal digesta were collected on days 5 and 6 of each period after 4 d of adaptation to diets. Results from experiment 1 indicated that ProCap DDGS contained more CP and AA compared with de-oiled DDGS. The SID of all AA in ProCap DDGS was greater (P < 0.001) compared with de-oiled DDGS with the exception that the SID of Pro was not different between the two sources of DDGS. In experiment 2, 24 growing barrows (initial BW: 32.7 ± 3.1 kg) were housed individually in metabolism crates and used in a randomized complete block design and fed a corn-based diet or two diets containing corn and each source of DDGS with eight replicate pigs per diet. Fecal and urine samples were collected for 4 d after 7 d of adaptation. Results from experiment 2 indicated that concentration of ME in ProCap DDGS was greater (P < 0.05) compared with corn or de-oiled DDGS. In experiment 3, 32 growing barrows (initial BW: 20.2 ± 0.9 kg) were placed in metabolism crates and allotted to four diets with eight pigs per diet using a 2 × 2 factorial treatment arrangement. The de-oiled DDGS and ProCap DDGS were both included in a diet without microbial phytase and a diet with microbial phytase (500 units/kg diet). Pigs were adapted to the diets for 5 d and fecal samples were collected for 4 d. Results from experiment 3 indicated that inclusion of phytase in the diet containing ProCap DDGS increased (P < 0.05) the STTD of P, but addition of phytase to the de-oiled DDGS diet did not increase STTD of P (interaction, P < 0.001), but the STTD of P was greater (P < 0.05) in de-oiled DDGS compared with ProCap DDGS. In conclusion, ProCap DDGS has greater SID of AA and contains more ME, but has reduced STTD of P compared with conventional de-oiled DDGS.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 148-148
Author(s):  
Jessica P Acosta Medellin ◽  
Su A Lee ◽  
Hans H Stein

Abstract The objective of this experiment was to test if standardized ileal digestibility (SID) of AA in a new source of high-protein distillers dried grains with solubles (HP-DDGS) is greater than in conventional distillers dried grains with solubles (DDGS-CV). Nine barrows (initial BW: 67.2 ± 6.4 kg) with a T-cannula in the distal ileum were allotted to a triplicate 3 × 3 Latin Square design with 3 diets and 3 periods. Two diets included HP-DDGS or DDGS-CV as the sole source of CP and AA. A nitrogen-free diet was used to determine basal endogenous losses of CP and AA. The statistical model included ingredient as fixed effect and square, period, and animal as random effects. Results indicated that that HP-DDGS contained more CP and AA compared with DDGS-CV (Table 1). The SID of all AA in HP-DDGS were greater (P < 0.001) compared with DDGS-CV with the exception that the SID of Pro was not different between the 2 sources of DDGS. The greater concentrations of AA and values for SID of AA in HP-DDGS indicate that this new DDGS source has a very high quality and may be a great source of digestible AA in diets fed to pigs.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 224-224 ◽  
Author(s):  
Jung Y Sung ◽  
Beob G Kim

Abstract The objective was to determine standardized total tract digestibility (STTD) of calcium (Ca) in hatchery byproducts fed to nursery pigs. Ten barrows with mean body weight of 10.2 ± 0.7 kg were individually housed in metabolism crates. Four corn-based diets were formulated to contain 10% of infertile eggs (40% CP, 11.6% Ca, and 4,630 kcal/kg GE as-is), 10% of unhatched eggs (40% CP, 12.7% Ca, and 4,296 kcal/kg GE as-is), 25% of culled chicks (66% CP, 1.8% Ca, and 5,897 kcal/kg GE as-is), and 15% of a mixture of 3 ingredients aforementioned (56% CP, 6.2% Ca, and 5,342 kcal/kg GE as-is), respectively. A calcium-free diet was also prepared to measure basal endogenous losses of Ca. Ten animals were assigned to a replicated 5 × 4 Latin square design with 5 diets, and 4 periods. Each period consisted of a 5-d adaptation period and 5-d collection period. The marker to marker method was employed for total feces collection. The STTD of Ca in culled chicks (75.6%) was greater than that in the mixture (64.5%; P < 0.05). The ATTD and STTD of Ca in unhatched eggs (38.3 and 43.4%, respectively) were less than those in infertile eggs (48.5 and 53.3%, respectively; P < 0.05). Basal endogenous losses of Ca were determined at 659 mg/kg of dry matter intake. In conclusion, culled chicks had the greatest standardized total tract digestibility of calcium among test ingredients and standardized total tract digestible calcium in infertile eggs, unhatched eggs, culled chicks, and the mixture were 6.2, 5.5, 1.4, and 4.0%, respectively.


2005 ◽  
Vol 85 (3) ◽  
pp. 355-363 ◽  
Author(s):  
C. Kaufmann ◽  
W. C. Sauer ◽  
M. Cervantes ◽  
Y. Zhang ◽  
J. He ◽  
...  

Studies were carried out to determine the digestibilities of amino acids (AA) and energy in five sources of rice bran (RB). The sources of RB included two that were not further processed, a defatted, an extruded, and an atypical source of RB consisting mainly of starchy endosperm referred to as polish. Six barrows, average initial body weight 36.5 kg, were fitted with a simple T-cannula at the distal ileum, and fed six diets according to a 6 × 6 Latin square design. The five RB-containing diets contained 53.8% corn, 19.9% soybean meal, and 23.1% RB. The basal diet contained 71.0% corn and 26.3% soybean meal. The dietary allowance was provided at a rate of 4% (wt/wt) of the individual body weight determined at the initiation of each experimental period. Each experimental period comprised 11 d. Following a 7-d adaptation period, faeces were collected for 48 h and ileal digesta for 24 h in two 12-h periods. Chromic oxide was used as the digestibility marker. There was considerable variation in AA digestibilities among the RB samples. By aid of the difference method under the dietary conditions specified, the apparent ileal digestibilities of lysine, methionine, threonine, and tryptophan ranged from 62.6 to 82.2, 61.4 to 76.0, 58.4 to 82.8, and 58.8 to 82.6%, respectively. The digestibilities were lowest in the defatted source of RB and highest in polish. Not including polish of which the total tract energy digestibility was 88.2%, the digestibilities of the other samples ranged from 60.5 to 65.8%. A small proportion of the variation in AA digestibility can be attributed to the content of crude protein, fat and ash in RB. Key words: Amino acids, digestibility, energy, pigs, rice bran


2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Diego A Rodriguez ◽  
Su A Lee ◽  
Hans H Stein

Abstract Soybean expellers may be produced by dry extrusion and mechanical oil pressing of soybeans, but there is limited information about the nutritional value of expellers produced via this procedure. Therefore, 2 experiments were conducted to test the hypothesis that standardized ileal digestibility (SID) of CP and amino acids (AA), apparent total tract digestibility (ATTD) of energy and total dietary fiber (TDF), and concentrations of DE, ME, and NE are greater in soybean expellers than in soybean meal (SBM) when fed to growing pigs. Pigs were the offspring of Line 359 boars mated to Camborough females (Pig Improvement Company, Hendersonville, TN). In experiment 1, nine growing barrows (initial BW: 55.98 kg ± 13.75 kg) with T-cannulas installed in the distal ileum were allotted to 1 of 3 diets using a triplicated 3 × 3 Latin square design with 3 periods. Two diets were formulated to contain 35% soybean expellers or 33% SBM as the sole source of AA. A N-free diet was used to determine basal endogenous losses of AA. Ileal digesta were collected on days 6 and 7 of each 7-d period. Results indicated that the SID of most indispensable and dispensable AA were greater (P < 0.05) or tended (P < 0.10) to be greater in soybean expellers than in SBM. In experiment 2, a corn-based diet and 2 diets based on corn and each of the 2 soybean products were formulated. Twenty-four growing barrows (initial BW: 44.88 kg ± 2.17 kg) were allotted to 1 of the 3 diets with 8 pigs per diet. Urine and fecal samples were collected for 4 d after 5 d of adaptation. Results indicated that the ATTD of energy and TDF was not different between soybean expellers and SBM, but the ATTD of TDF in the 2 soybean products was greater (P < 0.05) than in corn. Concentrations of DE and ME in soybean expellers were greater (P < 0.05) compared with corn or SBM. Soybean expellers had greater (P < 0.05) calculated NE compared with SBM, but there was no difference in NE between corn and soybean expellers. In conclusion, values for SID of most AA and DE, ME, and NE in soybean expellers were greater than in SBM.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 72-72
Author(s):  
Chan Sol Park ◽  
Darryl Ragland ◽  
Olayiwola Adeola

Abstract Two experiments were conducted to determine the influence of sources or concentrations of dietary fiber in nitrogen-free diets (NFD) on basal ileal endogenous losses (BEL) of amino acids (AA) and subsequent standardized ileal digestibility (SID) of AA in feed ingredients for pigs derived from such BEL. In Exp. 1, 20 pigs (initial body weight = 46.5 ± 2.97 kg) were assigned to five diets in a quadruplicate 5 × 2 incomplete Latin square design with two periods. Three NFD containing cellulose (insoluble and fermentable fiber), inulin (soluble and fermentable fiber), or carboxymethyl cellulose (CMC; soluble and non-fermentable fiber) at 40 g/kg plus two diets containing soybean meal (SBM) or wheat distillers’ dried grains (WDDG) as the sole source of nitrogen were prepared. Pigs fed NFD containing CMC had greater (P < 0.05) BEL of AA, except for Arg and Pro, than those fed the other NFD. The SID of most AA in SBM and WDDG corrected by BEL of AA from NFD containing CMC were greater (P < 0.05) than those corrected by BEL of AA from the other NFD. Experiment 2 was conducted with 21 barrows (initial body weight = 34.1 ± 2.57 kg) assigned to seven diets in a triplicate 7 × 3 incomplete Latin square design with 3 periods. Seven diets consisted of three NFD containing 4, 8, or 12 g/kg cellulose and four diets containing corn, wheat, SBM, or canola meal as the sole source of nitrogen. There was no difference in the BEL of AA, except for Pro, among pigs fed NFD with increasing concentration of cellulose. The SID of AA in test ingredients were not affected by BEL of AA from NFD containing increasing concentration of cellulose. In conclusion, chemical characteristics of dietary fiber, but not concentration, affect the BEL of AA in pigs.


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