scholarly journals Crystalline amino acids do not influence calculated values for standardized ileal digestibility of amino acids in feed ingredients included in diets for pigs

2020 ◽  
Vol 98 (11) ◽  
Author(s):  
Maryane S F Oliveira ◽  
Jerubella J Abelilla ◽  
Neil W Jaworski ◽  
John K Htoo ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Latin Square ◽  
Experimental Period ◽  
Adaptation Period ◽  
Ileal Digestibility ◽  
Entire Experiment ◽  
Collection Period ◽  
Dietary Treatments ◽  
Feeding Diets ◽  
Crystalline Amino Acids

Abstract An experiment was conducted to test the hypothesis that addition of crystalline amino acids (AA) to diets during the adaptation or collection periods will not influence calculated values for apparent ileal digestibility (AID) or standardized ileal digestibility (SID) of AA in corn and soybean meal (SBM). Seven ileal-cannulated barrows (initial body weight: 77.9 ± 2.6 kg) were allotted to a 7 × 7 Latin Square design with 7 dietary treatments and 7 periods. Dietary treatments included feeding diets based on corn or SBM without or with crystalline AA for the entire 7-d period or with crystalline AA during the adaptation period, but without crystalline AA during the collection period. An N-free diet was also used. Each experimental period consisted of 5 d of adaptation to the diets followed by 2 d of ileal digesta collection, with digesta being collected for 9 hr/d starting after feeding the morning meal. Thus, the entire experiment lasted 49 d. The AID and SID of crude protein (CP) and AA were calculated using values determined in corn or in SBM without or with crystalline AA to determine if crystalline AA influenced calculated values for AID or SID of CP and AA. Results indicated that addition of crystalline AA to diets fed during the entire 7-d period increased (P < 0.05) AID of some AA in corn and SBM. However, no differences in SID of CP and AA were observed between pigs fed the diets without crystalline AA and pigs fed the diets with crystalline AA if dietary crystalline AA were ignored in calculations of SID values. This indicates that crystalline AA were 100% absorbed before the distal ileum and did not affect calculated values for SID of AA if calculations were based only on the AA in corn or SBM. Therefore, it is concluded that crystalline AA may be added to experimental diets in digestibility experiments before and during collection periods without affecting results, if crystalline AA are disregarded in the calculation of AID or SID of AA in ingredients.

PSVI-13 Amino Acid Supplementation During the Adaptation Period Did Not Affect the Standardized Ileal Digestibility of Amino Acids in Corn and Soybean Meal Fed to Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 200-201
Author(s):  
Hyunjun Choi ◽  
Sun Jong You ◽  
Beob Gyun G Kim
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Latin Square ◽  
Sole Source ◽  
Adaptation Period ◽  
Amino Acid Supplementation ◽  
Ileal Digestibility ◽  
Indispensable Amino Acids ◽  
Dietary Treatments

Abstract The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P < 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P < 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.

scholarly journals PSVIII-34 Late-Breaking Abstract: Additivity of digestible amino acids in hatchery byproducts fed to nursery pigs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 343-345
Author(s):  
Beob G Kim ◽  
Jung Yeol Sung
Keyword(s):  
Amino Acids ◽  
Latin Square ◽  
Sole Source ◽  
Adaptation Period ◽  
Experimental Diet ◽  
Nursery Pigs ◽  
Ileal Digestibility ◽  
Collection Period ◽  
The Individual ◽  
Digestible Amino Acids

Abstract The objectives were to determine standardized ileal digestibility (SID) of amino acids (AA) in hatchery byproducts fed to nursery pigs and to test the additivity of ileal digestible AA concentrations in hatchery byproducts. Ten barrows (initial body weight = 11.3 ± 1.3 kg) surgically fitted with T-cannulas at the distal ileum were individually housed in pens. Four diets were prepared to contain 30% of infertile eggs, unhatched eggs, culled chicks, or a mixture (20% dried infertile eggs + 20% dried unhatched eggs + 60% dried culled chicks) as the sole source of nitrogen. A nitrogen-free diet was additionally prepared to measure basal endogenous losses of AA. All experimental diet contained 0.5% chromic oxide as an indigestible index. Ten pigs were assigned to a replicated 5 × 4 Latin square design with 5 diets and 4 periods. Each period consisted of a 5-d adaptation period and a 2-d collection period. The hypothesis was that the determined digestible AA concentrations of the mixture are not different from the calculated values based on digestible AA in the individual ingredients. A t-test was used to determine the additivity of digestible AA concentrations in individual ingredients. Infertile eggs had greater (P < 0.05) SID of all indispensable AA except for lysine and methionine compared with culled chicks. The SID of all indispensable AA except for tryptophan was not different between culled chicks and the mixture. The calculated apparent and standardized ileal digestible AA in the mixture were not different from the determined values, but calculated ileal digestible tryptophan in the mixture was less (P < 0.05) than the measured value. In conclusion, ileal digestibility of most indispensable AA in infertile eggs is greater than that in culled chicks and ileal digestible AA values are additive in the mixture except for tryptophan.

Digestibility of amino acids in pigs fed distillers dried grains with solubles derived from corn, sorghum, and triticale

2020 ◽  
Author(s):  
Chan Sol Park ◽  
Darryl Ragland ◽  
O. Adeola
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Latin Square ◽  
Experimental Period ◽  
Growing Pigs ◽  
Initial Body Weight ◽  
Isoleucine Leucine ◽  
Ileal Digestibility ◽  
Distillers Dried Grains ◽  
Dietary Treatments

An experiment was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in distillers dried grains with solubles derived from corn (C-DDGS), sorghum (S-DDGS), and triticale (T-DDGS) fed to growing pigs. A total of 16 barrows (initial body weight = 23.1 ± 2.03 kg) surgically fitted with T-cannulas at the distal ileum were divided into 4 groups based on body weight and assigned to a quadruplicated 4 × 2 incomplete Latin Square design with 4 dietary treatments and 2 experimental periods. Three diets containing C-DDGS, S-DDGS, or T-DDGS and a nitrogen-free diet were fed in each experimental period consisting of 5-d adaptation and 2-d ileal digesta collection. Pigs fed S-DDGS had less (P < 0.05) SID of histidine, isoleucine, leucine, and methionine compared with those fed T-DDGS, which were less (P < 0.05) than the values observed from pigs fed C-DDGS. The SID of arginine, phenylalanine, threonine, and valine in C-DDGS were greater (P < 0.05) than in S-DDGS but were not different from those in T-DDGS. The SID of lysine in C-DDGS was greater (P < 0.05) than in S-DDGS and T-DDGS. In conclusion, AA in S-DDGS were less digestible than those in C-DDGS and T-DDGS.

Nutritional evaluation of fish protein hydrolysate and its application in piglet production

2021 ◽  
Author(s):  
Nan Zhang ◽  
Xiaoming Song ◽  
Wenxuan Dong ◽  
Ling Liu ◽  
Zhiying Cui ◽  
...  
Keyword(s):  
Amino Acids ◽  
Antioxidant Capacity ◽  
Protein Hydrolysate ◽  
Nutrient Digestibility ◽  
Adaptation Period ◽  
Fish Protein ◽  
Experimental Diet ◽  
Ileal Digestibility ◽  
Fish Protein Hydrolysate ◽  
Dietary Treatments

Abstract This study was conducted to determine the digestible energy (DE), metabolizable energy (ME), and ileal digestibility of amino acids (AA) of fish protein hydrolysate (FPH), and to evaluate the effects of FPH on the performance of piglets. In Exp. 1, the available energy content of FPH was determined by difference methods: 12 barrows with an initial body weight (BW) of 40.0±2.1kg were randomly assigned into two dietary treatments with six replicates per treatment, the treatments contained a corn basal diet and an experimental diet in which 20% corn was replaced by FPH. The experiment was lasted for 12 days, with 7 days adaptation period followed by 5 days total collection of feces and urine. In Exp. 2, the standardized ileal digestibility (SID) of AA in FPH by the N-free method was determined, 12 barrows (initial BW of 35.3±1.8 kg) with ileal T-cannulas were randomly allotted into two treatments with six replicates per treatment, the dietary treatments consisted of an experimental diet and a N-free diet. The experiment was lasted for 7 days, with 5 days adaptation period followed by 2 days collection of ileal digesta. In Exp. 3, the effect of FPH on the performance of piglets was conducted. 192 piglets (initial BW of 8.10±1.8kg) were randomly allotted into four treatments with six replicates per treatment. The treatments were a control diet with fish meal (FM) and part or all FM was replaced by FPH, got diets containing 2%, 3% or 5% FPH. The experiment lasted for 28 days. Results showed that: In Exp. 1, the DE and ME of FPH were 21.12MJ/kg and 20.28MJ/kg. In Exp. 2, the SID of Lys, Met, Thr and Trp were 79.99%, 87.17%, 68.29%, and 71.53% respectively. In Exp. 3, addition of 3% FPH increased nutrient digestibility and volatile fatty acid content in feces. Addition of 5% FPH increased the average daily feed intake (ADFI), significantly increased (P&lt;0.05) the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and immunoglobulin A (IgA), while significantly decreased (P&lt;0.05) the average daily gain (ADG). In conclusion, FPH had a high value of digestible energy and ileal digestible essential amino acids, could improve nutrient digestibility, immunity, antioxidant capacity, and intestinal health of piglets, while adverse to nitrogen deposition in piglets.

scholarly journals 199 Effect of the Pelleting Process on Diet Formulations with Varying Levels of Crystalline Amino Acids and Reducing Sugars on Digestibility in Growing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 66-67
Author(s):  
Kara M Dunmire ◽  
Diego A Lopez ◽  
Chance J Fiehler ◽  
Yiqin Zhang ◽  
Cassandra K Jones ◽  
...  
Keyword(s):  
Amino Acids ◽  
Reducing Sugars ◽  
Latin Square ◽  
Growing Pigs ◽  
Ileal Digestibility ◽  
Distillers Grains With Solubles ◽  
Feed Form ◽  
Low Crystalline ◽  
Crystalline Amino Acids ◽  
Main Effects

Abstract The objective of this study was to determine effects of pelleting on the standardized ileal digestibility (SID) of amino acids (AA) in diets with or without increased concentrations of free AA and reducing sugars (RS). Eight individually housed, ileal cannulated barrows (initially 69.2 kg) were allotted to a replicated 8×8 Latin square with 8 diets and eight 7-d periods with ileal digesta collected on d 6 and 7. Treatments were arranged in a 2×2×2 factorial with main effects of diet form (mash vs. pellet), crystalline AA (low vs. high), or reducing sugars (low vs. high) provided by dried distillers grains with solubles and bakery meal. Diets were pelleted to achieve a hot pellet temperature of 85 to 88°C. Data were analyzed as a completely randomized Latin square using the GLIMMIX procedure of SAS. A feed form×RS interaction (P &lt; 0.026) for SID of tryptophan was observed. Feeding pelleted low RS diets improved SID of tryptophan compared with mash high and low RS diets, and pelleted high RS diets. For main effects of feed form, the SID of total AA, CP, and indispensable AA increased (P &lt; 0.042) in pigs fed pelleted diets compared with mash diets. For main effects of crystalline AA, pigs fed high crystalline AA had increased (P = 0.007) SID of tryptophan and decreased (P = 0.050) SID of histidine compared with those fed low crystalline AA diets. For main effects of RS diets, pigs fed high RS diets had decreased (P &lt; 0.05) SID of total AA, CP and indispensable AA. In conclusion, pelleting diets with increased crystalline AA or RS did not affect the improvement in AA digestibility from pelleting. Pelleting diets improved AA digestibility. Diets formulated with high crystalline AA had increased SID of tryptophan. Formulating diets with high RS resulted in decreased AA digestibility compared with corn-soybean meal-based diets.

Amino acid and energy digestibility in different sources of rice bran for growing pigs

2005 ◽  
Vol 85 (3) ◽  
pp. 355-363 ◽  
Author(s):  
C. Kaufmann ◽  
W. C. Sauer ◽  
M. Cervantes ◽  
Y. Zhang ◽  
J. He ◽  
...  
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Rice Bran ◽  
Soybean Meal ◽  
Latin Square ◽  
Basal Diet ◽  
Experimental Period ◽  
Growing Pigs ◽  
Adaptation Period ◽  
The Individual

Studies were carried out to determine the digestibilities of amino acids (AA) and energy in five sources of rice bran (RB). The sources of RB included two that were not further processed, a defatted, an extruded, and an atypical source of RB consisting mainly of starchy endosperm referred to as polish. Six barrows, average initial body weight 36.5 kg, were fitted with a simple T-cannula at the distal ileum, and fed six diets according to a 6 × 6 Latin square design. The five RB-containing diets contained 53.8% corn, 19.9% soybean meal, and 23.1% RB. The basal diet contained 71.0% corn and 26.3% soybean meal. The dietary allowance was provided at a rate of 4% (wt/wt) of the individual body weight determined at the initiation of each experimental period. Each experimental period comprised 11 d. Following a 7-d adaptation period, faeces were collected for 48 h and ileal digesta for 24 h in two 12-h periods. Chromic oxide was used as the digestibility marker. There was considerable variation in AA digestibilities among the RB samples. By aid of the difference method under the dietary conditions specified, the apparent ileal digestibilities of lysine, methionine, threonine, and tryptophan ranged from 62.6 to 82.2, 61.4 to 76.0, 58.4 to 82.8, and 58.8 to 82.6%, respectively. The digestibilities were lowest in the defatted source of RB and highest in polish. Not including polish of which the total tract energy digestibility was 88.2%, the digestibilities of the other samples ranged from 60.5 to 65.8%. A small proportion of the variation in AA digestibility can be attributed to the content of crude protein, fat and ash in RB. Key words: Amino acids, digestibility, energy, pigs, rice bran

scholarly journals 118 Digestibility of amino acids in hydrolyzed feather meal, flash dried poultry protein, poultry meal, and meat and bone meal fed to broiler chickens and pigs

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 65-65
Author(s):  
Chan Sol Park ◽  
Victor Naranjo ◽  
Ariane Helmbrecht ◽  
John K Htoo ◽  
Olayiwola Adeola
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Block Design ◽  
Latin Square ◽  
Experimental Period ◽  
Meat And Bone Meal ◽  
Feather Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Randomized Complete Block Design

Abstract This study was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in hydrolyzed feather meal (HFM), flash dried poultry protein (FDPP), poultry meal (PM), and meat and bone meal (MBM) fed to broiler chickens and pigs. Four semi-purified diets and a nitrogen-free diet were prepared. Exp. 1 was conducted with 416 male broiler chickens (initial body weight [BW] = 705 ± 100.0 g) at d 18 post-hatching in a randomized complete block design. Birds were fed diets for 5 d and then euthanized to collect ileal digesta. In Exp. 2, ten barrows (initial BW = 22.1 ± 1.59 kg) surgically fitted with T-cannulas at the distal ileum were assigned to duplicate 5 × 4 incomplete Latin square design with 5 diets and 4 periods. There were 5-d adaptation and 2-d ileal digesta collection periods in each experimental period. Data were analyzed as a 2 × 4 factorial arrangement with the effects of species (i.e., broiler chickens and pigs) and four experimental diets. There were interactions (P < 0.05) in the SID of His, Lys, Thr, Trp, and Val between diets and species. In broiler chickens, the SID of Lys in MBM was greater (P < 0.05) than HFM and FDPP but was not different from PM. Pigs fed MBM had greater (P < 0.05) SID of Lys than HFM, FDPP, and PM. In broiler chickens, the SID of His, Thr, and Trp in FDPP and PM were greater (P < 0.05) than HFM but was less (P < 0.05) than MBM. In pigs, the SID of His, Thr, and Trp in FDPP and PM were greater (P < 0.05) than HFM but was not different from MBM. In conclusion, differences in the SID of AA among animal protein sources were affected by species of monogastric animals.

scholarly journals Torula yeast has greater digestibility of amino acids and phosphorus, but not energy, compared with a commercial source of fish meal fed to weanling pigs

2020 ◽  
Vol 98 (1) ◽  
Author(s):  
L Vanessa Lagos ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Fish Meal ◽  
Latin Square ◽  
Sole Source ◽  
Adaptation Period ◽  
Weanling Pigs ◽  
Ileal Digestibility ◽  
Torula Yeast ◽  
Microbial Phytase ◽  
Commercial Source

Abstract Three experiments were conducted to test the hypothesis that the standardized ileal digestibility (SID) of AA, concentrations of DE and ME, and the standardized total tract digestibility (STTD) of P in a source of torula yeast are not different from values obtained in Menhaden fish meal. In experiment 1, six weanling barrows (initial BW: 11.7 ± 0.4 kg) were prepared with a T-cannula in the distal ileum and allotted to a replicated 3 × 3 Latin square design with 3 diets and 3 periods. In each period, there were 5 d of adaptation and 2 d of collection. Two cornstarch-based diets using the torula yeast or fish meal as the sole source of AA and a N-free diet were formulated. The SID of CP and all AA was greater (P &lt; 0.05) in torula yeast than in fish meal. In experiment 2, 24 weanling barrows (initial BW: 14.4 ± 1.1 kg) were individually housed in metabolism crates and allotted to a corn-based diet or 2 diets based on a mixture of corn and torula yeast or corn and fish meal. Feces and urine samples were collected for 4 d following a 5-d adaptation period. There were 8 replicate pigs per diet and fecal and urine materials were collected. Results of this experiment indicated that there were no differences in the concentration of DE and ME (DM basis) between torula yeast and fish meal. In experiment 3, a total of 32 weanling barrows (initial BW: 11.9 ± 1.1 kg) were allotted to 4 diets and 8 replicate pigs per diet. Pigs were placed in individual metabolism crates. The torula yeast or fish meal were used in 2 diets containing either 0 or 500 units of microbial phytase. Feces samples were collected as described for experiment 2. The STTD of P in torula yeast was greater (P &lt; 0.05) than in fish meal, but regardless of ingredient, there was no effect of the inclusion of phytase in the diets. In conclusion, the SID of AA and the STTD of P in torula yeast is greater than in fish meal, but values for the concentration of DE and ME in torula yeast are not different from those in fish meal. Therefore, the torula yeast that was used in the present experiments may be included at the expense of fish meal in diets fed to weanling pigs if the concentration of standardized ileal digestible AA is considered in the formulation.

Variability of apparent ileal amino acid digestibility in canola meal for growing-finishing pigs

1996 ◽  
Vol 76 (4) ◽  
pp. 563-569 ◽  
Author(s):  
M. Z. Fan ◽  
W. C. Sauer ◽  
V. M. Gabert
Keyword(s):  
Amino Acids ◽  
Crude Protein ◽  
Corn Starch ◽  
Latin Square ◽  
Experimental Period ◽  
Neutral Detergent Fiber ◽  
Canola Meal ◽  
Finishing Pigs ◽  
Analysis Method ◽  
Ileal Digestibility

Studies were conducted with six barrows, average initial body weigh 32.5 kg, fitted with a T-cannula at the distal ileum, to determine some of the factors responsible for the variability in apparent ileal digestibility values of amino acids (AA) in canola meal. The barrows were fed six corn starch-based diets, formulated to contain 18.0% crude protein (CP) on a dry matter (DM) basis from six canola meal samples, according to a 6 × 6 Latin square design. Chromic oxide (0.3%) was used as a digestibility marker. The pigs were fed twice daily, at 08:00 and 20:00 h. The dietary allowance was 1600 g d−1. Each experimental period consisted of 8 d. Ileal digesta were collected at 2-h intervals, for a total of 24 h from 08:00 on day 6 to 08:00 h on day 8. With the exception of proline, there were differences (P < 0.05) in the apparent ileal digestibility values of all AA among the six canola meal samples. Of the indispensable AA in canola meal, the digestibility values of arginine, histidine, and methionine were relatively high, ranging from 79.4 to 84.4, from 76.5 to 81.0%, and from 77.3 to 82.4%, respectively, while the digestibility values of threonine and tryptophan were relatively low, ranging from 59.7 to 66.5 and from 61.7 to 67.5%, respectively. Furthermore, with the exception of arginine, the digestibility values of the indispensable AA were negatively correlated (P < 0.05) with the neutral-detergent fiber (NDF) content in canola meal. In addition with the exception of threonine, tryptophan and valine, the digestibility values of the indispensable AA were positively correlated (P < 0.05) with their respective dietary levels. In conclusion, measured with the ileal analysis method, there were differences (P < 0.05) in the digestibility values of the majority of AA among the canola meal samples. Differences in NDF content were mainly responsible for the variation. In addition, differences in the dietary levels of most AA also contributed, in part, to the variation of AA digestibility values. Key words: Amino acids, ileal digestibility, canola meal, pigs

scholarly journals 181 Effects of Aspergillus oryzae prebiotic on energy and nutrient digestibility of growing pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 80-80
Author(s):  
Jinlong Zhu ◽  
Gerald C Shurson ◽  
Lynsey Whitacre ◽  
Ignacio R Ipharraguerre ◽  
Pedro E Urriola
Keyword(s):  
Amino Acids ◽  
Aspergillus Oryzae ◽  
Nutrient Content ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Adaptation Period ◽  
Ileal Digestibility ◽  
High Fiber ◽  
Wheat Middlings ◽  
Corn Distillers

Abstract The objective of this study was to determine the effects of an Aspergillus oryzae prebiotic (AOP, Amaferm®) on nutrient digestibility in growing pigs fed high fiber diets. Eighteen growing barrows (initial BW = 50.60 ± 4.90 kg) were surgically equipped with a T-cannula at the distal ileum. Three diets were formulated by including 29.65% corn-distillers dried grains with solubles (DDGS), 36.65% rice bran (RB) or 24.59% wheat middlings (WM) in corn and soybean meal-based diets to meet nutrient requirements for 50 to 75 kg growing pigs. Three additional diets were formulated by supplementing 0.05% AOP at the expense of corn in DDGS (DDGS + AOP), RB (RB + AOP), and wheat middlings (WM + AOP) diets. Pigs were allotted randomly to a triplicated 6 × 2 Youden square design with 6 diets and 2 successive periods. Feces and ileal digesta were collected for 2 d after a 21 d adaptation period, and nutrient content was analyzed to calculate apparent total tract digestibility (ATTD) and apparent ileal digestibility (AID). Standardized ileal digestibility (SID) of amino acids was calculated by correcting AID with basal endogenous amino acid losses determined from the same set of pigs. Supplementation of 0.05% AOP increased (P &lt; 0.05) ATTD of DM, GE, CP, NDF, and ash in DDGS, RB, and WM diets. Diet DE was 35 kcal/kg greater (P &lt; 0.05) in pigs fed AOP supplemented diets compared with those fed diets without AOP. Pigs fed DDGS+AOP diet had greater (P &lt; 0.05) AID of ether extract compared to those fed DDGS diet. However, supplementation of AOP did not (P &gt; 0.05) affect AID of GE, DM, CP, NDF, ash or SID of amino acids. In conclusion, supplementation of AOP in high fiber diets containing DDGS, RB, or WM increased total tract energy value and nutrient digestibility.

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