scholarly journals Intrinsic phytase in hybrid rye increases the digestibility of phosphorus in corn and soybean meal in diets fed to growing pigs

2020 ◽  
Vol 98 (10) ◽  
Author(s):  
Joan L Archs Toledo ◽  
Su A Lee ◽  
Molly L McGhee ◽  
Gonzalo G Mateos ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that inclusion of hybrid rye in diets containing corn and soybean meal (SBM) without or with microbial phytase improves the apparent total tract digestibility (ATTD) and the standardized total tract digestibility (STTD) of P because of the intrinsic phytase activity in hybrid rye. Forty-eight growing barrows (initial body weight: 39.5 ± 7.7 kg) were allotted to six diets. A basal diet containing corn and SBM; a rye-based diet; and a diet containing corn, SBM, and rye were formulated. Each diet was formulated without and with microbial phytase (500 units/kg of diet) for a total of six diets. Fecal samples were collected for 4 d following a 5-d adaptation period according to the marker-to-marker procedure. Results indicated that no interactions between diets and concentration of phytase were observed for any of the response criteria measured. The ATTD and STTD of P and the ATTD of Ca differed (P < 0.05) among diets, but regardless of diet, the concentration of P in feces was reduced (P < 0.05) by adding microbial phytase to the diets. As a consequence, microbial phytase increased (P < 0.05) ATTD and STTD of P, and the ATTD of Ca was also increased (P < 0.05) by the use of microbial phytase. Measured values for the ATTD and STTD of P in the diets containing corn, SBM, and hybrid rye without or with phytase were greater (P < 0.05) than values that were predicted based on the ATTD and STTD of P for the corn–SBM and the hybrid rye diet. The observation that STTD predicted from the individual ingredients underestimated the STTD of P in the mixed diet indicates that the intrinsic phytase in hybrid rye resulted in increased digestibility of the P in the corn and SBM included in the corn–SBM–hybrid rye diet. In conclusion, microbial phytase increased the ATTD and STTD of P and the ATTD of Ca regardless of feed ingredients used in diets fed to pigs. In addition, the intrinsic phytase from hybrid rye increased the ATTD and STTD of P in corn and SBM.

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Diego A Rodriguez ◽  
Su A Lee ◽  
María R C de Godoy ◽  
Hans H Stein

Abstract Two experiments were conducted to determine effects of extrusion on energy and nutrient digestibility in soybean hulls. One source of soybean hulls was ground and divided into two batches. One batch was used without further processing, whereas the other batch was extruded. In Exp. 1, four diets were formulated to determine crude protein (CP) and amino acid (AA) digestibility in soybean hulls. A soybean meal-based diet in which soybean meal provided all the CP and AA was formulated. Two diets were formulated to contain 30% nonextruded or extruded soybean hulls and 18% soybean meal. An N-free diet that was used to determine the endogenous losses of CP and AA was also used. Eight growing barrows (initial body weight = 37.0 ± 3.9 kg) had a T-cannula installed in the distal ileum and were allotted to a replicated 4 × 4 Latin square design. Each experimental period lasted 7 d with the initial 5 d being the adaptation period and ileal digesta were collected for 8 h on day 6 and 7. Results indicated that extrusion of soybean hulls did not change the standardized ileal digestibility (SID) of CP and most AA with the exception that the SID of Ile and Leu tended (P < 0.10) to be greater in extruded than nonextruded soybean hulls. In Exp. 2, three diets were formulated to determine energy digestibility in soybean hulls. One corn-soybean meal based basal diet, and two diets that contained corn, soybean meal, and 32% extruded or nonextruded soybean hulls were formulated. Twenty-four growing barrows (initial body weight = 59.9 ± 3.4 kg) were allotted to a randomized complete block design. Pigs were housed individually in metabolism crates and feces and urine were collected separately for 4 d after 5 d of adaptation. The apparent total tract digestibility (ATTD) of gross energy (GE) and the digestible energy (DE) and metabolizable energy (ME) were reduced (P < 0.05) in diets containing nonextruded or extruded soybean hulls compared with the basal diet. However, the ATTD of GE and values for DE and ME in soybean hulls were not improved by extrusion. Likewise, extrusion did not change the concentration of total dietary fiber in soybean hulls. In conclusion, there were no effects of extrusion of soybean hulls on SID of AA, energy digestibility, or ME concentration in soybean hulls.


2005 ◽  
Vol 85 (3) ◽  
pp. 355-363 ◽  
Author(s):  
C. Kaufmann ◽  
W. C. Sauer ◽  
M. Cervantes ◽  
Y. Zhang ◽  
J. He ◽  
...  

Studies were carried out to determine the digestibilities of amino acids (AA) and energy in five sources of rice bran (RB). The sources of RB included two that were not further processed, a defatted, an extruded, and an atypical source of RB consisting mainly of starchy endosperm referred to as polish. Six barrows, average initial body weight 36.5 kg, were fitted with a simple T-cannula at the distal ileum, and fed six diets according to a 6 × 6 Latin square design. The five RB-containing diets contained 53.8% corn, 19.9% soybean meal, and 23.1% RB. The basal diet contained 71.0% corn and 26.3% soybean meal. The dietary allowance was provided at a rate of 4% (wt/wt) of the individual body weight determined at the initiation of each experimental period. Each experimental period comprised 11 d. Following a 7-d adaptation period, faeces were collected for 48 h and ileal digesta for 24 h in two 12-h periods. Chromic oxide was used as the digestibility marker. There was considerable variation in AA digestibilities among the RB samples. By aid of the difference method under the dietary conditions specified, the apparent ileal digestibilities of lysine, methionine, threonine, and tryptophan ranged from 62.6 to 82.2, 61.4 to 76.0, 58.4 to 82.8, and 58.8 to 82.6%, respectively. The digestibilities were lowest in the defatted source of RB and highest in polish. Not including polish of which the total tract energy digestibility was 88.2%, the digestibilities of the other samples ranged from 60.5 to 65.8%. A small proportion of the variation in AA digestibility can be attributed to the content of crude protein, fat and ash in RB. Key words: Amino acids, digestibility, energy, pigs, rice bran


Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1168
Author(s):  
Lu Wang ◽  
Qile Hu ◽  
Peili Li ◽  
Changhua Lai ◽  
Defa Li ◽  
...  

The study was conducted to develop and validate an equation to predict the metabolizable energy (ME) of double-low rapeseed cakes (DLRSC) for growing pigs based on their chemical compositions. In Experiment 1, 66 growing pigs (initial body weight 36.6 ± 4.1 kg) were allotted randomly to a completely randomized design with 11 diets. The diets included a corn–soybean meal basal diet and 10 test diets containing 19.22% DLRSC supplemented at the expense of corn, soybean meal, and lysine. Neutral detergent fiber (NDF), crude fiber (CF), and gross energy (GE) were the best predictors to determine ME. The best-fit prediction equation of ME (MJ/kg) was ME = 9.33 − 0.09 × NDF − 0.25 × CF + 0.59 × GE (R2 = 0.93). In Experiment 2, a total of 144 growing pigs (initial body weight 29.7 ± 2.7 kg), with six pigs per pen and six pens per treatment, were assigned randomly to four treatments in a completely randomized block design for a 28-day feeding trial. A corn–soybean meal basal diet was prepared, and three additional diets were formulated by adding 7%, 14%, and 21% DLRSC to the basal diet at the expense of soybean meal. All diets were formulated to provide equal standardized ileal digestibility (SID) Lys/ME ratio and SID essential amino acids/SID Lys ratio. Increasing dietary levels of DLRSC had no effect on average daily feed intake, average daily gain, and feed-to-gain ratio. The caloric efficiency of ME (31.83, 32.44, 31.95, and 32.69 MJ/kg, respectively) was not changed by increasing the dietary concentration of DLRSC. Increasing dietary levels of DLRSC linearly reduced (p < 0.05) the concentrations of triiodothyronine and tetraiodothyronine in serum, as well as apparent total tract digestibility of DM, GE, crude protein, acid detergent fiber, and organic matter of the diet. In conclusion, the ME prediction equation obtained in Experiment 1 accurately estimates the ME value of DLRSC fed to growing pigs.


2020 ◽  
Vol 98 (12) ◽  
Author(s):  
Charmaine D Espinosa ◽  
Maryane S F Oliveira ◽  
L Vanessa Lagos ◽  
Terry L Weeden ◽  
Aileen J Mercado ◽  
...  

Abstract Three experiments were conducted to test the hypothesis that the standardized ileal digestibility (SID) of amino acids (AA), concentrations of digestible energy (DE) and metabolizable energy (ME), and the standardized total tract digestibility (STTD) of P in a new source of fermented soybean meal (Fermex 200) are greater than in conventional soybean meal (SBM-CV). In experiment 1, 9 barrows (initial body weight: 9.17 ± 1.03 kg) were surgically fitted with a T-cannula in the distal ileum and allotted to a triplicated 3 × 3 Latin square design. A nitrogen-free diet and 2 diets that contained cornstarch and SBM-CV or Fermex 200 as the sole source of crude protein (CP), and AA were formulated. Results indicated that there were no difference between SBM-CV and Fermex 200 for SID of CP and AA. In experiment 2, 24 growing pigs (initial body weight: 14.19 ± 1.18 kg) were housed individually in metabolism crates. Pigs were allotted to a corn-based diet or 2 diets that contained corn and SBM-CV or corn and Fermex 200. Feces and urine samples were collected using the marker-to-marker approach with 5-d adaptation and 4-d collection periods. Results indicated that the concentration of DE and ME in Fermex 200 were not different from DE and ME in SBM-CV. In experiment 3, 40 barrows (initial body weight: 11.01 ± 1.38 kg) were allotted to 1 of 4 diets with 10 replicate pigs per diet. Four diets were formulated to contain Fermex 200 or SBM-CV and either 0 or 1,000 units/kg of microbial phytase. Pigs were housed individually in metabolism crates. Fecal samples were collected as explained for experiment 2. Results indicated that the STTD of P in Fermex 200 was greater (P &lt; 0.01) than in SBM-CV, but the addition of microbial phytase to the diets increased the ATTD and STTD of P in SBM-CV, but not in Fermex 200 (interaction; P &lt; 0.01). In conclusion, the SID of AA and concentrations of DE and ME in Fermex 200 were not different from values determined for SBM-CV, but the STTD of P was greater in Fermex 200 than in SBM-CV if microbial phytase was not added to the diet.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 61-61
Author(s):  
Woongbi Bi Kwon ◽  
Jose A Soto ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that increasing concentrations of dietary Val, Ile, or Trp alone or in combination will alleviate negative effects of excess dietary Leu on N balance of growing pigs. Seventy-two barrows (initial body weight: 33.9 ± 2.6 kg) were housed in metabolism crates and randomly assigned to 1 of 8 diets and 3 blocks with 3 pigs per diet in each block in a 12-d experiment. Treatments were arranged in a 2 × 2 × 2 factorial with the main effects of L-Val (0 or 0.1%), L-Ile (0 or 0.1%), and L-Trp (0 or 0.05%) that were added to a basal diet containing corn and a high-protein corn product (48% crude protein; 5.9% Leu). The basal diet contained 1.00 % standardized ileal digestible (SID) Lys and 171% SID Leu:Lys. Data were analyzed using the PROC MIXED of SAS with concentrations of Val, Ile, and Trp, and all interactions as main effects and replicate as a random effect. No 3-way interactions were observed (Table 1). Results indicated that fecal N output increased if Ile was added to diets without added Val, but that was not the case if Val was added (interaction, P &lt; 0.05). Addition of Ile to diets reduced N retention, but N retention increased with Trp addition to diets without Val addition, but not if Trp was added to diets with added Val (interaction, P &lt; 0.05). The biological value of protein increased if Trp was added to diets without addition of Ile, but if Ile was added, Trp addition did not increase the biological value of protein (interaction, P &lt; 0.05). In conclusion, adding Ile to a diet with excess Leu reduced N retention, but if Trp was added alone or in combination with Ile or Val, N retention increased.


Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 228 ◽  
Author(s):  
Zhengqun Liu ◽  
Ruqing Zhong ◽  
Liang Chen ◽  
Fei Xie ◽  
Kai Li ◽  
...  

This study was conducted to evaluate the effect of collection durations on the energy values and nutrient digestibility of high-fiber diets in growing pigs with a time-based total fecal collection method. A total of 24 barrows (body weight (BW): 31.1 ± 1.5 kg) were allotted to a completely randomized design with three diets. Diets included a corn–soybean meal (CSM) basal diet and two additional diets containing 20% sugar beet pulp (SBP) or defatted rice bran (DFRB) by replacing corn, soybean meal, and soybean oil in the CSM diet, respectively. Each diet was fed to eight barrows for a 7-day adaptation period followed by a 7-day total feces and urine collection period. The 7-day collection duration was divided into three collection phases, namely, phase 1 (days 8 to 11), phase 2 (days 11 to 13), and phase 3 (days 13 to 15). Then, similar portions of feces and urine from the different collection phases were composited into three additional samples (days 8 to 11, days 8 to 13, and days 8 to 15, respectively). The results showed that the digestible energy (DE), metabolizable energy (ME), and apparent total tract digestibility (ATTD) of gross energy (GE) and nutrient in experimental diets decreased linearly as the collection durations increased from a 3-day to a 7-day collection (p < 0.05). However, there were no differences in the energy values, GE, and nutrient digestibility of diets and of high-fiber ingredients between the 5-day and 7-day collection durations. In conclusion, this study suggests that a 5-day collection duration is adequate to determine the energy values and nutrient digestibility of high-fiber diets containing SBP or DFRB in growing pigs by the time-based total fecal collection method.


2019 ◽  
Vol 97 (8) ◽  
pp. 3440-3450 ◽  
Author(s):  
Su A Lee ◽  
L Vanessa Lagos ◽  
Carrie L Walk ◽  
Hans H Stein

Abstract Two experiments were conducted to test the hypothesis that standardized total tract digestibility (STTD) of Ca and the response to microbial phytase is constant among different sources of Ca carbonate and that the STTD of Ca is constant among different sources of dicalcium phosphate (DCP) when fed to growing pigs. In Exp. 1, 80 pigs (initial BW: 19.0 ± 1.9 kg) were randomly allotted to 10 diets and 2 blocks with 4 pigs per diet in each block. Four sources of Ca carbonate were used, and each source was included in a diet without microbial phytase and a diet with microbial phytase (500 units/kg diet). Two Ca-free diets without or with microbial phytase were also formulated. Feed allowance was 2.7 times the maintenance energy requirement for ME and daily feed allotments were divided into 2 equal meals. The initial 4 d of each period were considered the adaptation period to the diets followed by 4 d of fecal collection using the marker-to-marker procedure. Pigs fed diets containing exogenous phytase had lower (P < 0.05) basal endogenous loss of Ca compared with pigs fed diets containing no phytase. There were no interactions between phytase and source of Ca carbonate. Values for STTD of Ca were greater (P < 0.05) for diets containing microbial phytase (77.3% to 85.4%) compared with diets without exogenous phytase (70.6% to 75.2%), and values for STTD of Ca differed (P < 0.05) among the 4 sources of Ca carbonate. In Exp. 2, 40 pigs (initial BW: 14.9 ± 1.3 kg) were allotted to a completely randomized design with 5 diets and 8 replicate pigs per diet. A basal diet in which all Ca was supplied by Ca carbonate was formulated. Three diets were formulated by adding 3 sources of DCP to the basal diet and a Ca-free diet was also used. Feeding and collection methods were as described for Exp. 1. Results indicated that values for STTD of Ca and ATTD of P were not different among diets, indicating that under the conditions of this experiment, the digestibility of Ca and P in DCP appears to be constant regardless of origin of DCP. In conclusion, use of microbial phytase reduces the basal endogenous loss of Ca and increases Ca digestibility in Ca carbonate. The STTD of Ca varies among sources of Ca carbonate, regardless of phytase inclusion, but that appears not to be the case for the STTD of Ca in different sources of DCP.


2020 ◽  
Vol 98 (10) ◽  
Author(s):  
Diego A Rodriguez ◽  
Su A Lee ◽  
Hans H Stein

Abstract Two experiments were conducted to test the hypothesis that the digestibility of gross energy (GE) and nutrients, and concentrations of digestible energy (DE) and metabolizable energy (ME) in two sources of distillers dried grains with solubles (DDGS) are not different despite different concentrations of fat in the two sources. Cold-fermented DDGS (6.82% fat) and a conventional DDGS (9.54% fat) were used. In experiment 1, 12 growing barrows (initial body weight = 55.2 ± 3.6 kg) that had a T-cannula installed in the distal ileum were allotted to one of three diets and two periods. Two diets contained either cold-fermented or conventional DDGS as the sole source of crude protein (CP) and amino acids (AA). The third diet was an N-free diet that was used to determine the basal endogenous losses of AA from the pigs. Each experimental period lasted 7 d and ileal digesta were collected on days 6 and 7 of each period. Results demonstrated that values for the standardized ileal digestibility (SID) of CP and most AA were greater (P &lt; 0.05) or tended to be greater (P &lt; 0.10) in cold-fermented than in conventional DDGS. In experiment 2, 24 barrows (initial body weight = 17.3 ± 1.3 kg) were randomly allotted to three diets with 8 replicate pigs per diet. A corn-based basal diet and two diets containing corn and either cold-fermented DDGS or conventional DDGS were formulated. Pigs were housed individually in metabolism crates and feces and urine were collected separately for 5 d after 7 d of adaptation. The apparent total tract digestibility (ATTD) of neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid-hydrolyzed ether extract (AEE) was greater (P &lt; 0.01) in conventional DDGS than in cold-fermented DDGS, but there was no difference in ATTD of GE between the two sources of DDGS. However, conventional DDGS contained more (P &lt; 0.001) DE and ME than cold-fermented DDGS because of greater GE. In conclusion, the SID of AA was greater in cold-fermented DDGS than in the conventional DDGS that was evaluated in this experiment, but the ATTD of NDF, ADF, and AEE, and ME were greater in conventional DDGS than in cold-fermented DDGS.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 116-117
Author(s):  
Jinsu Hong ◽  
Jung Wook Lee ◽  
Dan Pettersson ◽  
Tofuko A Woyengo

Abstract Objective of the study was to determine the effect of supplementing a combination of phytase and multi-carbohydrase on standardized ileal digestibility (SID) of amino acid (AA) and net energy (NE) value of carinata meal for growing pigs. Ten ileal-cannulated barrows (initial body weight = 53.9 ± 4.76 kg) were fed 4 diets in a replicated 4 × 4 Latin square design with 2 additional columns to give 10 replicates per diet. Diets included a corn-soybean meal (SBM)-based basal diet, the basal diet with 25% carinata meal, basal diet with 25% carinata meal plus phytase at 2,000 FTU/kg and multi-carbohydrase at 0.2g/kg, and a nitrogen-free diet. The multi-carbohydrase supplied 4 units of xylanase, 10 units of β-glucanase, and 1000 units of pectinase per kilogram of diet. The ratio of corn to soybean meal and soybean oil in carinata meal- containing diets was identical to that in the corn-soybean meal based basal diet to allow calculation of AA and energy digestibility of carinata meal by the difference method. On a DM basis, carinata meal contained 50.2% CP, 0.88% ether extract, 15.37% ADF, 1.82% Lys, 0.96% Met, 1.89% Thr, and 0.64% Trp, respectively. The SID of Lys, Met, Thr, and Trp for carinata meal were 51.4%, 82.2%, 65.9%, and 85.9%, respectively. Supplementation of a combination of phytase and multi-carbohydrase did not affect the SID of AA for the corn-SBM-carinata meal-based diet, and for the carinata meal. However, supplementation of a combination of phytase and multi-carbohydrase improved (P = 0.015) the apparent total tract digestibility of gross energy, and DE and NE values for carinata meal by 9.4, 9.5, and 12.4% respectively. In conclusion, the enzymes used in the current study could be added in carinata meal-based diets for pigs to improve the energy value of the carinata meal.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 173-174
Author(s):  
Su A Lee ◽  
Carrie L Walk ◽  
Hans H Stein

Abstract The objective was to test the hypothesis that standardized total tract digestibility (STTD) of Ca and the response to microbial phytase is constant among different sources of calcium carbonate and different sources of dicalcium phosphate (DCP). In Exp. 1, 80 pigs (19.0 ± 1.9 kg) were placed in metabolism crates and randomly allotted to 10 diets. Four calcium carbonate-containing diets and a Ca-free diet were formulated without or with microbial phytase (500 units/kg diet). Feces were collected for 4 d after a 5 d adaptation period. Data were analyzed using a model that included calcium carbonate source, phytase, and the interaction between source and phytase as fixed effects and replicate as random effect. There were no interactions between phytase and source. The basal endogenous loss (BEL) of Ca from pigs fed phytase was less (P = 0.037) than from pigs fed no phytase. Values for STTD of Ca in calcium carbonate differed (P < 0.05) among the 4 sources, but increased (P < 0.05) if phytase was used (Table 1). In Exp. 2, 40 pigs (14.9 ± 1.3 kg) were allotted to a basal diet where all Ca was supplied by calcium carbonate, 3 diets containing calcium carbonate and DCP, and a Ca-free diet. Pigs were placed in metabolism crates and feces were collected as in Exp. 1. Data were analyzed using a model that included DCP-source as fixed effect and replicate as random effect. Results indicated that the STTD of Ca in DCP was not different among sources. In conclusion, use of microbial phytase reduces the BEL of Ca and increases Ca digestibility in calcium carbonate, but the STTD of Ca varies among sources of calcium carbonate. However, no difference in STTD of Ca among sources of DCP were observed.


Sign in / Sign up

Export Citation Format

Share Document