scholarly journals PSIII-18 Standardized total tract digestibility of Ca by growing pigs in different sources of calcium carbonate and dicalcium phosphate

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 173-174
Author(s):  
Su A Lee ◽  
Carrie L Walk ◽  
Hans H Stein
Keyword(s):  
Calcium Carbonate ◽  
Fixed Effects ◽  
Random Effect ◽  
Basal Diet ◽  
Growing Pigs ◽  
Dicalcium Phosphate ◽  
Adaptation Period ◽  
Microbial Phytase ◽  
Carbonate Source ◽  
Different Sources

Abstract The objective was to test the hypothesis that standardized total tract digestibility (STTD) of Ca and the response to microbial phytase is constant among different sources of calcium carbonate and different sources of dicalcium phosphate (DCP). In Exp. 1, 80 pigs (19.0 ± 1.9 kg) were placed in metabolism crates and randomly allotted to 10 diets. Four calcium carbonate-containing diets and a Ca-free diet were formulated without or with microbial phytase (500 units/kg diet). Feces were collected for 4 d after a 5 d adaptation period. Data were analyzed using a model that included calcium carbonate source, phytase, and the interaction between source and phytase as fixed effects and replicate as random effect. There were no interactions between phytase and source. The basal endogenous loss (BEL) of Ca from pigs fed phytase was less (P = 0.037) than from pigs fed no phytase. Values for STTD of Ca in calcium carbonate differed (P < 0.05) among the 4 sources, but increased (P < 0.05) if phytase was used (Table 1). In Exp. 2, 40 pigs (14.9 ± 1.3 kg) were allotted to a basal diet where all Ca was supplied by calcium carbonate, 3 diets containing calcium carbonate and DCP, and a Ca-free diet. Pigs were placed in metabolism crates and feces were collected as in Exp. 1. Data were analyzed using a model that included DCP-source as fixed effect and replicate as random effect. Results indicated that the STTD of Ca in DCP was not different among sources. In conclusion, use of microbial phytase reduces the BEL of Ca and increases Ca digestibility in calcium carbonate, but the STTD of Ca varies among sources of calcium carbonate. However, no difference in STTD of Ca among sources of DCP were observed.

scholarly journals Standardized total tract digestibility of calcium varies among sources of calcium carbonate, but not among sources of dicalcium phosphate, but microbial phytase increases calcium digestibility in calcium carbonate1

2019 ◽  
Vol 97 (8) ◽  
pp. 3440-3450 ◽  
Author(s):  
Su A Lee ◽  
L Vanessa Lagos ◽  
Carrie L Walk ◽  
Hans H Stein
Keyword(s):  
Energy Requirement ◽  
Basal Diet ◽  
Growing Pigs ◽  
Dicalcium Phosphate ◽  
Adaptation Period ◽  
Randomized Design ◽  
Microbial Phytase ◽  
Formulated Feed ◽  
Ca Carbonate ◽  
Different Sources

Abstract Two experiments were conducted to test the hypothesis that standardized total tract digestibility (STTD) of Ca and the response to microbial phytase is constant among different sources of Ca carbonate and that the STTD of Ca is constant among different sources of dicalcium phosphate (DCP) when fed to growing pigs. In Exp. 1, 80 pigs (initial BW: 19.0 ± 1.9 kg) were randomly allotted to 10 diets and 2 blocks with 4 pigs per diet in each block. Four sources of Ca carbonate were used, and each source was included in a diet without microbial phytase and a diet with microbial phytase (500 units/kg diet). Two Ca-free diets without or with microbial phytase were also formulated. Feed allowance was 2.7 times the maintenance energy requirement for ME and daily feed allotments were divided into 2 equal meals. The initial 4 d of each period were considered the adaptation period to the diets followed by 4 d of fecal collection using the marker-to-marker procedure. Pigs fed diets containing exogenous phytase had lower (P < 0.05) basal endogenous loss of Ca compared with pigs fed diets containing no phytase. There were no interactions between phytase and source of Ca carbonate. Values for STTD of Ca were greater (P < 0.05) for diets containing microbial phytase (77.3% to 85.4%) compared with diets without exogenous phytase (70.6% to 75.2%), and values for STTD of Ca differed (P < 0.05) among the 4 sources of Ca carbonate. In Exp. 2, 40 pigs (initial BW: 14.9 ± 1.3 kg) were allotted to a completely randomized design with 5 diets and 8 replicate pigs per diet. A basal diet in which all Ca was supplied by Ca carbonate was formulated. Three diets were formulated by adding 3 sources of DCP to the basal diet and a Ca-free diet was also used. Feeding and collection methods were as described for Exp. 1. Results indicated that values for STTD of Ca and ATTD of P were not different among diets, indicating that under the conditions of this experiment, the digestibility of Ca and P in DCP appears to be constant regardless of origin of DCP. In conclusion, use of microbial phytase reduces the basal endogenous loss of Ca and increases Ca digestibility in Ca carbonate. The STTD of Ca varies among sources of Ca carbonate, regardless of phytase inclusion, but that appears not to be the case for the STTD of Ca in different sources of DCP.

scholarly journals PSIX-4 Concentrations of digestible and metabolizable energy and digestibility of phosphorus in a new source of high-protein distillers dried grains with solubles fed to growing pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 187-188
Author(s):  
minoy A Cristobal Romero ◽  
Su A Lee ◽  
Hans H Stein
Keyword(s):  
Statistical Model ◽  
Fixed Effects ◽  
Random Effect ◽  
Growing Pigs ◽  
Metabolizable Energy ◽  
High Protein ◽  
Experimental Unit ◽  
Distillers Dried Grains ◽  
Microbial Phytase

Abstract Concentrations of digestible and metabolizable energy and digestibility of phosphorus in a new source of high-protein distillers dried grains with solubles fed to growing pigs Minoy Cristobal, Su A Lee, and Hans H. Stein The objective of this research was to test the hypothesis that concentrations of DE and ME and standardized total tract digestibility (STTD) of P in high-protein distillers dried grains with solubles (HP-DDGS) are greater than in conventional distillers dried grains with solubles (DDGS-CV). In Exp. 1, twenty-four growing barrows (initial BW: 32.7 ± 3.1 kg) were fed a corn diet or 2 diets containing corn and HP-DDGS or corn and DDGS-CV. The statistical model included ingredient as a fixed effects and replicate as the random effect and the pig was the experimental unit. Results from Exp. 1 indicated that the concentrations of DE and ME of HP-DDGS were greater (P &lt; 0.05) compared with DDGS-CV (Table 1). In Exp. 2, thirty-two growing barrows (initial BW: 20.2 ± 0.9 kg) were allotted to 4 diets with 8 pigs per diet. The DDGS-CV or HP-DDGS were included in a diet without microbial phytase and in a diet with microbial phytase (500 units/kg diet). The statistical model included ingredient, phytase, and the interaction as fixed effects and replicate as the random effect. Results from Exp. 2 indicated that inclusion of phytase in the diet containing HP-DDGS increased (P &lt; 0.05) the STTD of P, but addition of phytase to the DDGS-CV diet did not increase STTD of P (interaction, P &lt; 0.001; Table 2). The value for the STTD of P was greater (P &lt; 0.05) in DDGS-CV compared with HP-DDGS. In conclusion, HP-DDGS has greater concentrations of DE and ME, but less STTD of P compared with DDGS-CV.

scholarly journals Intrinsic phytase in hybrid rye increases the digestibility of phosphorus in corn and soybean meal in diets fed to growing pigs

2020 ◽  
Vol 98 (10) ◽  
Author(s):  
Joan L Archs Toledo ◽  
Su A Lee ◽  
Molly L McGhee ◽  
Gonzalo G Mateos ◽  
Hans H Stein
Keyword(s):  
Body Weight ◽  
Soybean Meal ◽  
Basal Diet ◽  
Growing Pigs ◽  
Adaptation Period ◽  
Mixed Diet ◽  
Initial Body Weight ◽  
Microbial Phytase ◽  
Hybrid Rye ◽  
The Individual

Abstract An experiment was conducted to test the hypothesis that inclusion of hybrid rye in diets containing corn and soybean meal (SBM) without or with microbial phytase improves the apparent total tract digestibility (ATTD) and the standardized total tract digestibility (STTD) of P because of the intrinsic phytase activity in hybrid rye. Forty-eight growing barrows (initial body weight: 39.5 ± 7.7 kg) were allotted to six diets. A basal diet containing corn and SBM; a rye-based diet; and a diet containing corn, SBM, and rye were formulated. Each diet was formulated without and with microbial phytase (500 units/kg of diet) for a total of six diets. Fecal samples were collected for 4 d following a 5-d adaptation period according to the marker-to-marker procedure. Results indicated that no interactions between diets and concentration of phytase were observed for any of the response criteria measured. The ATTD and STTD of P and the ATTD of Ca differed (P &lt; 0.05) among diets, but regardless of diet, the concentration of P in feces was reduced (P &lt; 0.05) by adding microbial phytase to the diets. As a consequence, microbial phytase increased (P &lt; 0.05) ATTD and STTD of P, and the ATTD of Ca was also increased (P &lt; 0.05) by the use of microbial phytase. Measured values for the ATTD and STTD of P in the diets containing corn, SBM, and hybrid rye without or with phytase were greater (P &lt; 0.05) than values that were predicted based on the ATTD and STTD of P for the corn–SBM and the hybrid rye diet. The observation that STTD predicted from the individual ingredients underestimated the STTD of P in the mixed diet indicates that the intrinsic phytase in hybrid rye resulted in increased digestibility of the P in the corn and SBM included in the corn–SBM–hybrid rye diet. In conclusion, microbial phytase increased the ATTD and STTD of P and the ATTD of Ca regardless of feed ingredients used in diets fed to pigs. In addition, the intrinsic phytase from hybrid rye increased the ATTD and STTD of P in corn and SBM.

scholarly journals 282 Effect of formulating diets based on a ratio between STTD Ca and STTD P and the inclusion of phytase on the calcium and phosphorus balance of growing pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 103-104
Author(s):  
Vanessa Lagos ◽  
Mike R Bedford ◽  
Hans H Stein
Keyword(s):  
Block Design ◽  
Random Effect ◽  
Growing Pigs ◽  
Diet Formulation ◽  
Randomized Complete Block Design ◽  
Microbial Phytase ◽  
P Utilization ◽  
P Balance ◽  
Main Effects ◽  
P Absorption

Abstract An experiment was conducted to test the hypothesis that formulating diets for pigs based on a ratio between standardized total tract digestible (STTD) Ca and STTD P instead of total Ca and STTD P increases the efficiency of Ca and P utilization. Forty barrows (59.4 ± 3.8 kg) were individually housed in metabolism crates and allotted to 4 corn-soybean meal diets and 2 periods of 11 d in a randomized complete block design. Diets were formulated using a 2 × 2 factorial design with 2 Ca requirement estimates (total Ca or STTD Ca) and 2 inclusion levels of microbial phytase (0 or 500 units/kg). Phytase was assumed to release 0.11% STTD P and 0.16% total Ca. Diets were formulated based on requirements for total Ca and STTD P and the ratio between STTD Ca and STTD P was 1.25:1 in diets formulated based on STTD Ca. Fecal and urine samples were collected from feed provided from d 6 to 9. Data for Ca and P balance were analyzed using a model that included the main effects of diet formulation and phytase level, the interaction between main effects, and the random effect of period. Interactions (P &lt; 0.05) between diet formulation and phytase level were observed for Ca intake, Ca in feces, Ca digestibility, Ca retained as a percentage of intake, P digestibility, P absorbed, and P in urine (Table 1). Despite being provided less (P &lt; 0.05) Ca, pigs fed diets formulated based on STTD Ca did not absorb or retain less Ca than pigs fed total Ca diets, but they absorbed more (P &lt; 0.05) P than pigs fed diets formulated based on total Ca. In conclusion, by formulating diets based on values for STTD Ca, P absorption was increased confirming detrimental effects of oversupplying Ca.

PSIV-B-25 Digestible energy and metabolizable energy of high-fiber ingredients in growing pig diets

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 394-395
Author(s):  
Jongkeon Kim ◽  
Yun Yeong Jo ◽  
Beob Gyun G Kim
Keyword(s):  
Latin Square ◽  
Basal Diet ◽  
Growing Pigs ◽  
Metabolizable Energy ◽  
Adaptation Period ◽  
Digestible Energy ◽  
High Fiber ◽  
Cashew Nut ◽  
Growing Pig ◽  
Gluten Feed

Abstract The objective of this study was to determine the digestible energy (DE) and metabolizable energy (ME) concentrations in high-fiber ingredients fed to growing pigs. Twelve barrows with an initial body weight of 57.5 kg (SD = 5.7) were individually housed in metabolism crates. A replicated 6 × 3 incomplete Latin square design with 12 animals, 6 experimental diets and 3 periods was employed. A basal diet was composed of 75.0% corn and 22.7% soybean meal (SBM) as the sole energy sources. Four experimental diets were prepared by replacing 40% of corn and SBM with soybean hulls (SH), corn gluten feed (CGF), wheat bran (WB), or rice bran (RB). An additional diet was prepared by replacing 10% of corn and SBM with cashew nut hulls (CNH). Each period consisted of a 4-d adaptation period and a 4-d collection period, and the marker-to-marker procedure was used for total collection of feces and urine. The DE and ME values in RB (3,969 and 3,936 kcal/kg DM) were greater (P &lt; 0.05) than those in CGF (2,654 and 2,520 kcal/kg DM) and SH (2,492 and 2,541 kcal/kg DM) and the energy values in WB (3,162 and 3,118 kcal/kg DM) were not different from those in RB, CGF, or SH. The DE and ME values in CNH (350 and 572 kcal/kg DM) were less (P &lt; 0.05) than those in all other test ingredients. In conclusion, energy concentrations in RB were greatest among the high-fiber test ingredients, whereas CNH had the lowest values.

143 Effect of Supplementary Selenium Source on Dairy Cow Performance, Antioxidant Status, and Apparent Absorption and Retention

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 80-80
Author(s):  
Keira M Cruickshank ◽  
Bayissa Hatew ◽  
Amanda M Gehman ◽  
Karen M Koenig ◽  
Eduardo S Ribeiro ◽  
...  
Keyword(s):  
Dairy Cow ◽  
Fixed Effects ◽  
Antioxidant Status ◽  
Treatment Time ◽  
Rumen Fluid ◽  
Random Effect ◽  
Fecal Excretion ◽  
Adaptation Period ◽  
Apparent Absorption ◽  
Cow Performance

Abstract The objective of this experiment was to determine how source of selenium (Se) affects dairy cow performance, antioxidant status, and apparent absorption and retention. Multiparous Holstein cows (n = 24; 597 ± 49 kg BW) were blocked by days in milk (161 ± 18) and randomly assigned to receive 0.3 mg/kg of either: 1) organic Se (selenized yeast; ORG; Sel-Plex-Alltech); or, 2) inorganic Se (sodium selenite; INO) premix, top dressed and mixed into a ration daily. After an 11-week adaptation period (blood and milk sampled monthly), cows received an intraruminal dose of Se77 (either Se77 yeast or NaSe77O3) followed by a 4-day period of blood and rumen fluid sampling, and total collection of feces, urine, and milk. Data were analyzed using PROC GLIMMIX in SAS with fixed effects of treatment, time, and their interaction, and random effect of block. Daily DMI (23 ± 0.6 kg), milk yield (35 ± 1.2 kg), plasma glutathione peroxidase (64 ± 4.2 U), and serum Se (0.11 ± 0.003 µg/g) were not different between treatments during the adaptation period. Serum Se77 maximum concentration and area under the curve (AUC) were not different between treatments for 72 hours following infusion, but rumen fluid Se77 AUC was higher (P = 0.02) for ORG cows. Apparent absorption (64 ± 1.3%) and retention (44 ± 1.5%) of the Se77 dose did not differ between treatments. The ORG cows had lower urinary excretion (13 vs. 17 ± 0.6%; P &lt; 0.01), higher milk excretion (6 vs. 2 ± 0.3%; P &lt; 0.01), and similar fecal excretion (36 ± 1.3%; P = 0.9) of Se77 compared to INO cows. These results indicate that ORG Se increased Se content of milk and decreased Se excretion in urine, but did not alter performance, antioxidant status, and apparent absorption and retention of Se in mid-lactation cows.

171 Effects of Dietary Valine, Isoleucine, and Tryptophan Supplementations to Diets Containing Excess Leucine on Nitrogen Balance of Growing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 61-61
Author(s):  
Woongbi Bi Kwon ◽  
Jose A Soto ◽  
Hans H Stein
Keyword(s):  
Body Weight ◽  
Crude Protein ◽  
Random Effect ◽  
Basal Diet ◽  
Growing Pigs ◽  
Negative Effects ◽  
Initial Body Weight ◽  
N Retention ◽  
Biological Value ◽  
Main Effects

Abstract An experiment was conducted to test the hypothesis that increasing concentrations of dietary Val, Ile, or Trp alone or in combination will alleviate negative effects of excess dietary Leu on N balance of growing pigs. Seventy-two barrows (initial body weight: 33.9 ± 2.6 kg) were housed in metabolism crates and randomly assigned to 1 of 8 diets and 3 blocks with 3 pigs per diet in each block in a 12-d experiment. Treatments were arranged in a 2 × 2 × 2 factorial with the main effects of L-Val (0 or 0.1%), L-Ile (0 or 0.1%), and L-Trp (0 or 0.05%) that were added to a basal diet containing corn and a high-protein corn product (48% crude protein; 5.9% Leu). The basal diet contained 1.00 % standardized ileal digestible (SID) Lys and 171% SID Leu:Lys. Data were analyzed using the PROC MIXED of SAS with concentrations of Val, Ile, and Trp, and all interactions as main effects and replicate as a random effect. No 3-way interactions were observed (Table 1). Results indicated that fecal N output increased if Ile was added to diets without added Val, but that was not the case if Val was added (interaction, P &lt; 0.05). Addition of Ile to diets reduced N retention, but N retention increased with Trp addition to diets without Val addition, but not if Trp was added to diets with added Val (interaction, P &lt; 0.05). The biological value of protein increased if Trp was added to diets without addition of Ile, but if Ile was added, Trp addition did not increase the biological value of protein (interaction, P &lt; 0.05). In conclusion, adding Ile to a diet with excess Leu reduced N retention, but if Trp was added alone or in combination with Ile or Val, N retention increased.

scholarly journals Effects of Collection Durations on the Determination of Energy Values and Nutrient Digestibility of High-Fiber Diets in Growing Pigs by Total Fecal Collection Method

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 228 ◽  
Author(s):  
Zhengqun Liu ◽  
Ruqing Zhong ◽  
Liang Chen ◽  
Fei Xie ◽  
Kai Li ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Phase 1 ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Metabolizable Energy ◽  
Adaptation Period ◽  
Collection Method ◽  
High Fiber ◽  
Fecal Collection

This study was conducted to evaluate the effect of collection durations on the energy values and nutrient digestibility of high-fiber diets in growing pigs with a time-based total fecal collection method. A total of 24 barrows (body weight (BW): 31.1 ± 1.5 kg) were allotted to a completely randomized design with three diets. Diets included a corn–soybean meal (CSM) basal diet and two additional diets containing 20% sugar beet pulp (SBP) or defatted rice bran (DFRB) by replacing corn, soybean meal, and soybean oil in the CSM diet, respectively. Each diet was fed to eight barrows for a 7-day adaptation period followed by a 7-day total feces and urine collection period. The 7-day collection duration was divided into three collection phases, namely, phase 1 (days 8 to 11), phase 2 (days 11 to 13), and phase 3 (days 13 to 15). Then, similar portions of feces and urine from the different collection phases were composited into three additional samples (days 8 to 11, days 8 to 13, and days 8 to 15, respectively). The results showed that the digestible energy (DE), metabolizable energy (ME), and apparent total tract digestibility (ATTD) of gross energy (GE) and nutrient in experimental diets decreased linearly as the collection durations increased from a 3-day to a 7-day collection (p < 0.05). However, there were no differences in the energy values, GE, and nutrient digestibility of diets and of high-fiber ingredients between the 5-day and 7-day collection durations. In conclusion, this study suggests that a 5-day collection duration is adequate to determine the energy values and nutrient digestibility of high-fiber diets containing SBP or DFRB in growing pigs by the time-based total fecal collection method.

scholarly journals PSIV-13 Basal endogenous loss, standardized total tract digestibility, and retention of Ca in sows change throughout gestation, but microbial phytase reduces basal endogenous loss of Ca by gestating sows

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 185-186
Author(s):  
Su A Lee ◽  
Carrie L Walk ◽  
Hans H Stein
Keyword(s):  
Calcium Carbonate ◽  
Repeated Measures ◽  
Fixed Effects ◽  
Sole Source ◽  
Late Gestation ◽  
Phosphorus Retention ◽  
Early Gestation ◽  
Microbial Phytase ◽  
Period Of Gestation ◽  
P Retention

Abstract The objective was to test the hypothesis that standardized total tract digestibility (STTD) of Ca and Ca and P retention and the response to microbial phytase in diets fed to sows are constant throughout gestation. Thirty-six sows (parity = 3.3) were allotted to 4 diets on d 7 post-breeding. Two corn-based diets in which calcium carbonate was the sole source of Ca and 2 Ca-free diets were formulated without or with phytase (500 units per kg). Sows were housed individually in metabolism crates during early-gestation, mid-gestation, and late-gestation, and feces and urine were quantitatively collected. Data were analyzed by repeated measures using a model that included phytase, period of gestation, and the interaction between phytase and period as fixed effects, and block and replicate as random effects. Interactions between period and phytase were not observed. The basal endogenous loss (BEL) of Ca was greater (P < 0.05) in early-gestation than in mid- and late-gestation, but phytase reduced (P = 0.002) BEL of Ca and tended (P = 0.099) to increase apparent total tract digestibility (ATTD) of P in the Ca-free diet. Phytase did not affect ATTD of DM, STTD of Ca, ATTD of P, or Ca and P retention in sows fed the diet containing calcium carbonate (Table 1). The ATTD of DM was not affected by period, but Ca retention and ATTD of Ca and P were least (P < 0.05) in mid-gestation, followed by early- and late-gestation, and the STTD of Ca in mid-gestation was also reduced (P < 0.05) compared with early- or late-gestation. Phosphorus retention was greater (P < 0.05) in late-gestation than in the earlier periods. In conclusion, BEL of Ca, STTD of Ca, ATTD of P, and Ca and P retention in sows change throughout gestation regardless of use of phytase. http://www.conferenceharvester.com/

scholarly journals Application of a kinetic model to describe phosphorus metabolism in pigs fed a diet with a microbial phytase

2010 ◽  
Vol 148 (3) ◽  
pp. 277-286 ◽  
Author(s):  
R. S. DIAS ◽  
S. LOPEZ ◽  
J. A. MOREIRA ◽  
M. SCHULIN-ZEUTHEN ◽  
D. M. S. S. VITTI ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Specific Radioactivity ◽  
Negative Relationship ◽  
Basal Diet ◽  
Growing Pigs ◽  
Strongly Correlated ◽  
Microbial Phytase ◽  
Liver And Kidney ◽  
P Balance ◽  
Total P

SUMMARYThe objective of the current study was to apply the Vitti–Dias model to investigate phosphorus (P) metabolism in growing pigs fed a diet supplemented with microbial phytase. The basal diet contained maize, defatted rice bran, vegetable oil, soybean meal, limestone, salt and a vitamin and mineral mix. There was no inorganic P in the diet and phytase was added at levels of 253, 759, 1265 and 1748 phytase units (PU)/kg of feed. The compartmental model included four pools of P: (1) gut lumen, (2) plasma, (3) bone and (4) soft tissue. A single dose of 32P was administered, and specific radioactivity was measured in plasma, faeces, bone and soft tissue (muscle, heart, liver and kidney) at different times post-dosing for calculation of P flows between pools. Total P absorbed showed a negative relationship with total P excreted in faeces and was strongly correlated with bone P retention, suggesting that absorbed P was channelled to bone to address its physiological growth. Average efficiency of metabolic utilization of absorbed P was estimated to be 0·94, with 0·52 g/g of total net P balance being accreted in bone and the rest in soft tissue (including muscle and some vital organs). The Vitti–Dias model provided suitable representation of P interchange between compartments (in particular, flows between gut and plasma and partitioning of available P between bone and soft tissue), resulting in estimates of P flows comparable with values calculated from balance data.

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