115 Stability of Thiamine Supplied by Vitamin Premix and Yeast Ingredients in Canned Cat Food

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 61-62
Author(s):  
Amanda N Dainton ◽  
Markus F Miller ◽  
Brittany White ◽  
Leah K Lambrakis ◽  
Charles G Aldrich

Abstract Despite numerous process modifications, thiamine retention of canned cat food has not improved. Yeasts possess a binding protein which may provide a thiamine form able to withstand retort processing. Therefore, the objective was to evaluate dried yeasts as thiamine sources in canned cat food. Treatments were arranged as a 2x4 factorial with 2 levels of vitamin premix (with or without) and 4 sources of yeast (none (NY), LBV, BY, or EA). Inclusion of LBV matched the thiamine contribution from the vitamin premix and BY and EA were capped at 5%. Three replicates were processed in a horizontal still retort to an average total F0 of 79.23 minutes. Thiamine degradation was calculated as the difference between pre- and post-retort thiamine contents. Data were analyzed as a randomized complete block design with pre-retort thiamine content as a covariate and production day as a random effect. The Fisher’s LSD post hoc comparison test was performed with ⍺ = 0.05. On average, experimental formulas retained 33.75% thiamine. Thiamine degradation was not affected by the inclusion of vitamin premix (P = 0.0670), but was affected by the inclusion of yeast (P = 0.0232) and the interaction between vitamin premix and yeast (P = 0.0002). Thiamine loss between NY (-31.3 mg/kg DM) and BY (-33.8 mg/kg DM) were similar (P > 0.05) whereas EA (-40.5 mg/kg DM) and LBV (-55.6 mg/kg DM) lost more (P < 0.05) thiamine than NY. The treatment of EA with vitamin premix (-70.3 mg/kg DM) lost more (P < 0.05) thiamine than no yeast, BY, or EA without vitamin premix (average -17.4 mg/kg DM) and all others (average -57.3 mg/kg DM) were intermediate (P > 0.05). Inclusion of vitamin premix with yeast did not minimize thiamine loss and BY exhibited similar thiamine degradation as intrinsic thiamine in standard ingredients.

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 103-104
Author(s):  
Vanessa Lagos ◽  
Mike R Bedford ◽  
Hans H Stein

Abstract An experiment was conducted to test the hypothesis that formulating diets for pigs based on a ratio between standardized total tract digestible (STTD) Ca and STTD P instead of total Ca and STTD P increases the efficiency of Ca and P utilization. Forty barrows (59.4 ± 3.8 kg) were individually housed in metabolism crates and allotted to 4 corn-soybean meal diets and 2 periods of 11 d in a randomized complete block design. Diets were formulated using a 2 × 2 factorial design with 2 Ca requirement estimates (total Ca or STTD Ca) and 2 inclusion levels of microbial phytase (0 or 500 units/kg). Phytase was assumed to release 0.11% STTD P and 0.16% total Ca. Diets were formulated based on requirements for total Ca and STTD P and the ratio between STTD Ca and STTD P was 1.25:1 in diets formulated based on STTD Ca. Fecal and urine samples were collected from feed provided from d 6 to 9. Data for Ca and P balance were analyzed using a model that included the main effects of diet formulation and phytase level, the interaction between main effects, and the random effect of period. Interactions (P < 0.05) between diet formulation and phytase level were observed for Ca intake, Ca in feces, Ca digestibility, Ca retained as a percentage of intake, P digestibility, P absorbed, and P in urine (Table 1). Despite being provided less (P < 0.05) Ca, pigs fed diets formulated based on STTD Ca did not absorb or retain less Ca than pigs fed total Ca diets, but they absorbed more (P < 0.05) P than pigs fed diets formulated based on total Ca. In conclusion, by formulating diets based on values for STTD Ca, P absorption was increased confirming detrimental effects of oversupplying Ca.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 188-189
Author(s):  
Michael Coelho ◽  
Peter Ader

Abstract This study examined the effect of 1-monoglycerides and 1,2-diglycerides of butyric-, caproic- and caprylic acid (SILOHealth™ 104L [monoglycerides]) and avilamycin on day 14 and 21 post weaning performance of E. coli (GN-Ent and O157:H7) day 5 challenged piglets fed corn/soy diets that met or exceeded current NRC (2012) recommendations. A total of 96, Choice Genetics CG32, piglets were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (4 piglets/pen x 3 treatments x 8 replications). Pigs were blocked by sex and weight and were orally dosed with 5 ml (1x109 CFU/g, strain GN-Ent and O157:H7) E. coli d 5 after weaning (Ec). The treatment were: NC with Ec; T1=NC + 3 kg/MT monoglycerides; T2=NC+ 80 ppm avilamycin. Least significant difference was used to compare means of treatment. Mortalities and pen removals were weighed and recorded. The E. coli infection model was effective in causing E. coli mortality of 25% at d14 post weaning and 33% at d21 post weaning in NC. T1 and T2 reduced mortality versus NC at d14 (25, 3 and 0%, P < 0.05) and d21 (33, 4 and 0%, P < 0.05), increased ADG at d14 (203, 255 and 259 g, P < 0.05) and d21 (456, 511 and 515 g, P < 0.05), decreased FCR at d21 (1.90, 1.85 and 1.83, P < 0.05), and decreased intestinal E. coli, log 10 of CFU/g, versus NC (4.1, 2.2 and 2.1, P < 0.05). Monoglycerides and avilamycin performance, mortality and intestinal E. coli log 10 were not significantly different at P < 0.05. In conclusion, the use of monoglycerides improved ADG, reduced FCR, reduced mortality and reduced intestinal E. coli log 10 at day 14 and 21 post weaning versus NC and monoglycerides were not significant different (P < 0.05) than avilamycin.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 70-70
Author(s):  
Michael Coelho ◽  
Robert Jones ◽  
Frank Parks

Abstract This study evaluated the metabolizable (ME) release of graded levels of phytase when fed to 35 kg pigs on mash corn/soy diets formulated to meet or exceed current NRC (2012) recommendations. A total of 280 male (PIC 337 x Camborough) pigs were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (4 pig/pen x 8 treatments x 10 replications). Pigs were blocked by sex and weight. Treatments groups consisted of PC, NC1=PC- 0.17% Ca, - 0.15% aP, -0.02% Na, -200 kcal/kg, T1=NC +1000 FTU/kg phytase+0.15 g/kg NSPase, T2=NC+1500 FTU/kg phytase+0.15 g/kg NSPase, T3=NC+2000 FTU/kg phytase+0.15 g/kg NSPase, T4=NC+2500 FTU/kg phytase+0.15 g/kg NSPase and T5=NC+3000 FTU/kg phytase +0.15 g/kg NSPase. TiO2 was used as the undigestible marker. Feed, rectum grabbed fecal samples were collected at 35 kg pigs. ME was 3407, 3213, 3301, 3329, 3345, 3354 and 3358 Kcal/kg, P < 0.05, respectively. The NC reduced the ME by 194 Kcal/kg versus the PC. 1000, 1500, 2000, 2500 and 3000 FTU/kg released 87, 116, 132, 141 and 145 Kcal/kg, respectively. Least cost formulation reduced net feed costs by $8.63, $9.22, $11.10, $11.90 and $12.39/ton, respectively. In conclusion, Natuphos E by breaking down phytic acid and Natugrain TS by breaking down NSPs released up to 145 Kcal/kg ME in pigs.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 70-71
Author(s):  
Michael Coelho ◽  
Robert Jones ◽  
Frank Parks

Abstract This study presents a novel find of a Feed NSPase Enzyme, Natugrain TS, reducing finish pig hemorrhagic bowel syndrome (HBS) mortality. A total of 3,750 weaned pigs (28 kg), (PIC 337 x Camborough), were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (25 pigs/pen x 5 treatments x 30 replications). Pigs were blocked by sex and weight and were fed mash corn/soy/DDGs diets formulated to meet or exceed current NRC (2012) recommendations. Treatments were T1= 0 g/MT Natugrain TS, T2= 100 g/MT Natugrain TS, T3= 150 g/MT Natugrain TS, T4= 200 g/MT Natugrain TS and T5= 250 g/MT Natugrain TS. At the finishing phase, Natugrain TS significantly decreased intestinal E. coli log 10 (7.12, 4.95, 4.02, 3.21 and 3.25 log 10, P < 0.05, respectively), significantly decreased intestinal lesion score (0–5 score) (4.21, 4.02, 2.98, 2.25 and 2.26, P < 0.05, respectively). Microbiota was analyzed in fresh, manually collected, rectum fecal samples. Microbiota was separated into prevalent in Natugrain TS control (0) and 200 g/ton. In the control treatment, there was a prevalence of Desulfoyibrio, Oscillospira, Campylobacter, Anearoctruncus, Mogibacterium and Brachyspirae, etc., while in the 200 g/ton treatment there was a prevalence of Synergistales, Burkolderiales, Dehabacteriaceae, Bifidobacterium, Roseburia, Lactobacillus and Bacilli, etc. Natugrain TS significantly decreased mortality (4.8, 3.6, 2.7, 1.9 and 2.1 %, P < 0.05, respectively), significantly decreased specifically HBS mortality (3.50, 1.98, 0.68, 0.40 and 0.46%, P < 0.05, respectively). In conclusion, Natugrain TS changed the microbiome by breaking down arabinoxylans, decreasing intestinal E. coli and intestinal lesion score, changing the microbiota to prevalent Bifidobacterium, Roseburia, Lactobacillus and Bacilli, and Natugrain TS up to 200 g/ton specifically decreasing hemorrhagic bowel syndrome mortality from 3.5% to 0.5%.


Author(s):  
Rajesh Neupane ◽  
Subin Babu Neupane ◽  
Bhawana Acharya ◽  
Shikshya Parajuli ◽  
Nischal Oli ◽  
...  

Aims: Production of chili (Capsicum annuum Linnaeus; Solanaceae) in rain-fed regions of Nepal during the winter is constrained by the limited availability of soil moisture. Mulching has been a proven tool to conserve soil moisture and enhance yield. A field study was conducted in the winter of 2019 to identify the most suitable mulch that enhances the yield and yield attributing parameters of chili. Study Design: Seven treatments with three replications were evaluated under the randomized complete block design. Among the treatments, rice straw (5 kg/plot) and water reed (5 kg/plot) were used as natural mulches. While, plastic mulches: transparent (25 µ), silver (25 µ), black (25 µ), and black thick (50 µ) (double thickness) were used as synthetic mulches. Only soil with no mulch was the control. Place and Duration of Study: The study was conducted at Bangau, Dang, Nepal from November 2019 to May 2020. Methodology: We measured soil temperature at 10 cm depth using soil thermometer, and calculated soil moisture through oven dry method. Similarly, weight of ten fresh and ten dry fruits was recorded from each plot using an electronic scale. Number of fruits per plant was also counted. All the data were analyzed using ANOVA and means were separated following a post hoc test. Results: The highest soil moisture (18.38%), number of fruits per plant (24.91), fruit fresh weight per ten fruits (59.86 g), and fruit dry weight per ten fruits (14.65 g) were recorded in black thick plastic mulched plots. Furthermore, the lowest fresh weed weight (95.30 g) was also measured in black thick plastic mulched plots. Whereas, the highest soil temperature (18.92°C) was recorded in transparent plastic mulched plots. Conclusion: This study concludes black thick plastic as the most effective among the treatments tested in this study in enhancing yield and yield attributing parameters of chili.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 215-216
Author(s):  
Michael Coelho ◽  
Robert Jones ◽  
Frank Parks

Abstract There is a continued industry focus to decrease FCR (Pierozan, 2016), which means less feed per kg body tissue, and therefore vitamins need to be further concentrated in less and less feed to reach the same vitamin supplementation per kg of body tissue. A study was conducted to determine the response of pigs to different levels of vitamin supplementation based on the 2018 BASF Industry vitamin supplementation survey. Pigs were stressed to simulate commercial stress conditions. A total of 240 (PIC 337 x Camborough) pigs were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (4 pigs/pen x 5 treatments x 12 replications). Pigs were blocked by sex and weight and fed corn/soy diets pelleted at 80C and formulated to meet or exceed current NRC (2012) recommendations. The stress imposed to simulate commercial conditions consisted of dirty floors, 1.65 m2/pig, on 7th day of trial, pigs were dosed with 1,000,000 E. Coli bacteria and 200,000 Salmonella bacteria. Treatments were NRC, low 25%, AVG, high 25% and high 5% vitamin supplementation. At finishing, vitamin supplementation increased ADG up to high 5% vitamin supplementation (750, 795, 831, 852, and 871 g, P < 0.05, respectively), improved FCR up to Hi 5% (3.21, 3.05, 2.91, 2.82 and 2.71, P < 0.05, respectively). Vitamin supplementation decreased finishing mortality up to high 5% vitamin supplementation (9.02, 7.43, 6.15, 4.81 and 4.02%, P < 0.05, respectively), improved lean cuts up to Hi 25% (57.5, 59.1, 60.5, 61.3 and 62.1, P < 0.05, respectively). The profit in $/kg pig and return on extra vitamin supplementation was from low 25 to AVG $0.1381/kg and 39x, respectively, from AVG to Hi25% was $0.0771/kg and 13X, and from Hi 25% to Hi 5% was $0.1259/kg and 38X.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 187-188
Author(s):  
Michael Coelho ◽  
Robert Jones ◽  
Frank Parks

Abstract The objective of this experiment was to evaluate the metabolizable energy (ME)-releasing efficacy of a 6-phytase, Natuphos E, on pig performance compared with fat. A total of 1750 weaned, (PIC 337 x Camborough) pigs were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (25 pig/rep x 7 treatments x 10 replications). Pigs were blocked by sex and weight. Treatments consisted of PC, NC1=PC- 0.17% Ca, - 0.15% aP, -80 Kcal/kg, NC2=PC- 0.17% Ca, - 0.15% aP, -100 Kcal/kg, NC3=PC- 0.17% Ca, - 0.15% aP, -120 Kcal/kg, NC1 + 1000, NC2 + 1750 and NC3 + 2500 FTU/kg. Pigs were fed mash corn/soy diets formulated to meet or exceed current NRC (2012) recommendations. Least significant difference was used to compare means of treatments. In phase 3, ADG was 655, 650, 642, 632, 697, 911 and 724 g, P < 0.05, respectively. FI was 968, 1000, 1004, 1010, 1028, 1023 and 1052,P < 0.05, respectively. FCR was 1.48, 1.54, 1.56, 1.60, 1.47, 1.44 and 1.45, P < 0.05, respectively. NC1, NC2 and NC3 decreased (P < 0.05) ADG and increased (P < 0.05) FCR compared to PC. NC1 + 1000, NC2 + 1750 and NC3 + 2500 FTU/kg Natuphos E, increased (P < 0.05) ADG, and decreased (P < 0.05) FCR compared to PC. ADG, FI and FCR of NC1 + 1000, NC2 + 1750 and NC3 + 2500 had a better performance (P < 0.05) than NC1, NC2 and NC3, respectively. In conclusion, 1000, 1750 and 2500 FTU/kg phytase, Natuphos E, met or exceeded (P < 0.05) the performance of 80, 100 and 120 Kcal/kg. 2500 FTU/kg Natuphos E phytase could compensate, performance wise, the reduction of 0.17% Ca, 0.15% aP and 120 Kcal/kg ME in the diet.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 193-194
Author(s):  
Henrique S Cemin ◽  
Mike D Tokach ◽  
Steve S Dritz ◽  
Jason C Woodworth ◽  
Joel M DeRouchey ◽  
...  

Abstract A total of 3,171 pigs (PIC 327×L42; initially 5.8 kg) were used in a 39-d study. Treatments were arranged in a 2 × 2 factorial with 0 or 1% cellulose (Arbocel, J. Rettenmaier USA, Schoolcraft, MI) and distillers dried grains with solubles (DDGS; 0 or 5% in phase 1 and 0 or 15% in phase 2). Dietary phases 1 and 2 were offered from d 0 to 10 and 10 to 25, respectively. From d 25 to 39, pigs received a common diet with 25% DDGS. Pens were blocked by weight and allotted to treatments in a randomized complete block design. Experimental unit was two pens (66 pigs) sharing a fence-line feeder with 12 replicates per treatment. Data were analyzed with the GLIMMIX procedure of SAS with block as random effect. From d 0 to 25 and d 0 to 39, there was an interaction (P < 0.05) between cellulose and DDGS for ADG. Pigs fed diets with both DDGS and cellulose had lower ADG than those fed diets without DDGS, with pigs fed diets with DDGS without the addition of cellulose being intermediate. From d 25 to 39, there was a tendency (P = 0.080) for an interaction for ADFI. Pigs previously fed diets without DDGS and with cellulose had higher ADFI than those fed diets with DDGS and cellulose, and pigs previously fed diets without cellulose had similar ADFI regardless of DDGS inclusion. There was a tendency for an interaction (P = 0.070) for pig removals. Adding cellulose to diets without DDGS numerically decreased pig removals, but the inclusion of cellulose to diets with DDGS resulted in increased pig removals. In summary, adding fiber to the diet as cellulose or DDGS resulted in a less pig removals; however, adding both cellulose and DDGS decreased ADG and ADFI.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 188-188
Author(s):  
Michael Coelho ◽  
Robert Jones ◽  
Frank Parks

Abstract This study examined the effect of a phytase, Natuphos E, target dosing on pig performance and feed costs from wean to finish. A total of 240 (PIC 337 x Camborough), pigs were used in a randomized complete block design with pen as the experimental unit, treatment as the fixed effect, and block as the random effect (6 pig/pen x 2 treatments x 20 replications). Pigs were blocked by sex and weight equalized and fed mash corn/soy diets formulated to meet or exceed the current NRC (2012) recommendations. Treatments consisted of PC, NC1 in phase 1 and 2=PC- 0.16% Ca, - 0.14% aP, -0.02% Na + 3000 FTU/kg phytase. NC2 in phase 3 and grow-fin= PC- 0.16% Ca, - 0.14% aP, -0.02% Na, -120 Kcal/kg + 2500 FTU/kg phytase. Phase 2 ADG was 396 and 457 g/d, P < 0.05, respectively, and FCR was 1.31 and 1.25, P < 0.05, respectively. Phase 3 (11-23kg) ADG was 638 and 644 g, P < 0.05, respectively, and FCR was 1.52 and 1.48, P < 0.05, respectively. Finishing phase (120 kg) ADG was 871 and 875 g, P < 0.05, respectively, and FCR was 3.02 and 2.98, P < 0.05, respectively. In phase 1 and 2, only P, Ca and Na were removed from the diet, allowing AA and ME released from phytic acid to go into performance, ADG increased by 15% and FCR improved by 6 points, P < 0.05. In conclusion, by removing P in phase 1 and 2 and adding 3000 FTU/kg phytase, ADG and FCR were significantly improved, and net feed cost was reduced by $1.33/ton. In phase 3 and grow-fin, P and ME savings and adding 2500 FTU/kg phytase reduced net cost by $11.00/ton feed, while maintaining the pig performance.


2019 ◽  
Vol 97 (7) ◽  
pp. 2965-2971
Author(s):  
Laura Greiner ◽  
Amanda Graham ◽  
Marcio Goncalves ◽  
Uislei Orlando ◽  
Kevin J Touchette

Abstract An experiment was conducted with 430 sows to evaluate the ideal standardized ileal digestible (SID) Val:Lys on sow and litter performance during an 18.6-d lactation period. The SID Val:Lys ratios measured were based on the previously published literature and then values above and below reported values. Sows were randomly allotted within parity block (parity 1, parity 2, and parity 3+) to one of five corn–soybean meal-based lactation diets formulated to contain different levels of SID Val:Lys (0.50, 0.62, 0.75, 0.88, and 1.00, respectively). All diets were formulated to be isocaloric (3.35 ME Mcal/kg) with 0.95% SID Lys and contained vitamins and minerals that exceeded recommendations (NRC, 2012). Experimental diets were given to sows from 112 d of pregnancy throughout the 23-d lactation period. Litters were standardized within 48 h after farrowing. Sows were fed with the Howema computerized feeding system that mixed the high and low diets to create the intermediate diets, weighed the feed for each sow and then delivered the feed to each individual feeding hopper to record daily sow feed intake during lactation. Data were analyzed as a randomized complete block design using the PROC MIXED procedure of SAS with sow as the experimental unit and treatment as a fixed effect and parity as the random effect. Results were considered significant at P ≤ 0.05 and considered a trend at P > 0.05 and P ≤ 0.10. Sow ADFI was not significantly different (5.2, 5.2, 5.2, 5.0, and 5.4 ± 0.15 kg/d, respectively). On average, sows consumed ~45 g of SID Lys per day. Sow wean to estrus (5.2, 4.7, 5.3, 5.5, and 4.5 ± 0.32 d, respectively) was not different as the Val:Lys ratio changed. Increasing the Val:Lys ratio resulted in no differences (2.66, 2.64, 2.76, 2.61, and 2.62 ± 0.08 kg; P > 0.10) in average daily litter gain. There were no differences in sow weight loss or subsequent total born across the dietary treatments. Overall, increasing levels of SID Val:Lys did not change sow reproductive performance or piglet growth rate.


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