232 Effects of Yarrowia Lipolytica Supplementation on Growth Performance and Apparent Ileal Digestibility of Diets Fed to Nursery Pigs

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 118-118
Author(s):  
Yi-Chi Cheng ◽  
Marcos E Duarte ◽  
Sung Woo Kim

Abstract The objective was to evaluate Yarrowia lipolytica (YL) as a lipid supplement fed to nursery pigs for the growth performance and apparent ileal digestibility (AID). Twenty-four pigs weaned at 21 d of age (12 barrows and 12 gilts with initial BW at 7.2 ± 0.6 kg) were allotted to 3 dietary treatments (n = 8) based on the randomized complete block design with sex and BW as blocks. The main effect was the dietary supplementation of YL (0, 1.5, and 3.0%, replacing poultry fat) with nutrients meeting or exceeding the requirements by NRC (2012) and fed to pigs for 21 d based on 2 phases. Feed intake and BW were recorded at d 0, 10, and 21 to calculate ADG, ADFI, and G:F. Fecal scores were recorded at every odd day from d 3 to 19. Pigs were euthanized on d 21 to collect ileal digesta for AID of nutrients in diets. Data were analyzed using Proc Mixed of SAS. There were no differences in growth performance among treatments. Whereas, pigs fed 0 and 1.5% YL had higher (P < 0.05) AID of DM (55.0 and 52.2% to 40.2%, respectively) and GE (62.1 and 60.6% to 49.8%, respectively) than pigs fed 3% YL. There were no differences in AID of DM and GE between the pigs fed 0% and 1.5% YL. In conclusion, 1.5% Yarrowia lipolytica could be supplemented in nursery pig diets reducing 0.8% of poultry fat without affecting growth performance and AID of DM and GE.

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 371-371
Author(s):  
Caitlin E Evans ◽  
Marut Saensukjaroenphon ◽  
Haley Wecker ◽  
Jason C Woodworth ◽  
Cassandra K Jones ◽  
...  

Abstract A total of 320 pigs (DNA 241×600; initially 10.2 kg BW) were utilized in a 21-d experiment to determine the effects of corn fractionation and pelleting technique on nursery pig growth performance. There were 5 pigs per pen, 8 pens per treatment and 8 dietary treatments in a randomized complete block design. Treatments 1–3 contained 400 µm ground corn and were fed as either mash, pelleted using a steam conditioner plus traditional vertical ring die (steam pellet) or pelleted with hot water plus a horizontal die (cold pellet). Treatments 4–6 contained corn ground to 400 µm with fines < 150 um removed and were fed as either mash, steam pellet or cold pellet. Treatments 7 and 8 contained ground corn with only fines < 150 um steam or cold pelleted prior to dietary inclusion without complete diet pelleting. Overall, pigs fed mash diets had improved (P < 0.05) ADG and d 21 BW compared to those fed steam pelleted diets with those fed cold pelleted diets being intermediate. There was no difference in G:F between pigs fed mash, steam pellet and cold pellet diets; however, pigs fed diets containing pelleted fines had decreased (P < 0.05) G:F due to an observed increased feed wastage and sorting. There was no difference in growth performance between pigs fed diets with fines removed or not. Cold pelleting was a viable option to steam pelleting in the current experiment; however, pelleting diets reduced pig performance compared to pigs fed mash diets, which was unexpected. Further research is needed to validate the response to cold pelleting when the expected response to pelleting using steam conditioning is achieved.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 61-62
Author(s):  
Zhong-Xing Rao ◽  
Mike D Tokach ◽  
Steve S Dritz ◽  
Jason C Woodworth ◽  
Joel M DeRouchey ◽  
...  

Abstract A total of 350 pigs (241×600; DNA, Columbus, NE; initially 8.9 kg) were used in a 28-d study to determine the effect of fumonisin-contaminated corn on growth performance of nursery pigs. Dietary treatments consisted of fumonisin-contaminated corn blended with low fumonisin corn to provide analyzed toxin (FB1 + FB2) levels in the final diet of 7.2, 14.7, 21.9, 32.7, and 35.1 ppm. There were 5 pigs per pen and 14 pens per treatment. Pens were assigned to treatments in a randomized complete block design with initial weight as the blocking factor. Data were analyzed using lme package in R program with pen as the experimental unit. From d 0 to 28, increasing fumonisin decreased (linear, P< 0.05) ADG, ADFI, G:F, and final BW. Although responses tested linear, the greatest reduction in ADG and G:F was observed in pigs fed 32.7 and 35.1 ppm of fumonisin. Increasing fumonisin increased serum sphinganine to sphingosine (Sa:So) ratios (linear, P< 0.001) on day 14 and 28. Serum Sa:So ratio appears to be a useful biomarker indicating fumonisin intoxication. These results suggest that for 9- to 28-kg nursery pigs, diets containing 32 ppm of fumonisin or greater decreased growth performance and increased serum Sa:So ratio. Further research is warranted to determine the effects of fumonisin concentrations between 22 and 32 ppm.


Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 174 ◽  
Author(s):  
Jung Yeol Sung ◽  
Beob Gyun Kim

The objectives were to determine effects of a hatchery byproduct mixture (HBM) on growth performance and to measure digestible energy concentrations in various HBM. In the growth performance experiment, 96 pigs (initial body weight = 9.6 ± 0.8 kg) were assigned to 4 dietary treatments in a randomized complete block design with 6 blocks. Each treatment consisted of 6 replicate pens with 4 pigs comprising 2 barrows and 2 gilts. Pigs were fed graded concentrations of HBM at 0%, 3.33%, 6.67%, and 10.00% for 14 days. In the energy digestibility experiment, 10 barrows (initial body weight = 11.5 ± 0.4 kg) were employed to determine digestible energy in HBM. A basal diet based on corn and soybean meal and 4 additional diets containing 25% of 4 different HBM were prepared. The marker-to-marker method was employed for total collection and the experimental design was a replicated 5 × 4 Latins square design. Growth performance was not compromised as the inclusion rate of HBM increased up to 10%. Digestible energy of HBM ranged from 2772 to 3887 kcal/kg as-is basis. In conclusion, HBM can be used in nursery pig diets and different energy values should be used for each HBM.


2019 ◽  
Vol 97 (10) ◽  
pp. 4032-4040
Author(s):  
Carine M Vier ◽  
Steve S Dritz ◽  
Fangzhou Wu ◽  
Mike D Tokach ◽  
Joel M DeRouchey ◽  
...  

Abstract Two experiments were conducted to determine the standardized total tract digestible phosphorus (STTD P) requirement for 11- to 23-kg nursery pigs fed diets with or without phytase. A total of 1,080 and 2,140 pigs (PIC 359 × Camborough, Hendersonville, TN; initially 11.4 ± 0.29 and 11.1 ± 0.24 kg) were used in Exp. 1 and Exp. 2, respectively. There were 23 to 27 pigs per pen with 6 and 12 replicate pens per treatment in Exp. 1 and Exp. 2, respectively. After weaning, pigs were fed a common pelleted diet with 0.45% STTD P for 7 d, and a common phase 2 meal diet with 0.40% STTD P for 14 d in Exp. 1 and 18 d in Exp. 2. Pens of pigs were then allotted to dietary treatments in a randomized complete block design with body weight (BW) as the blocking factor. In Exp. 1, dietary treatments consisted of 0.26%, 0.30%, 0.33%, 0.38%, 0.43%, 0.48%, and 0.53% STTD P. Treatments were achieved with the inclusion of monocalcium phosphate at the expense of corn. In Exp. 2, diets contained 1,000 phytase units (FYT; Ronozyme Hiphos 2500, DSM Nutritional Products, Inc., Parsippany, NJ) with assumed release value 0.132% STTD P, and treatments consisted of 0.30%, 0.33%, 0.38%, 0.43%, 0.48%, 0.53%, and 0.58% STTD P. These STTD P concentrations included the expected phytase release of 0.132% STTD P. In both experiments, a similar 1.17:1 Ca:P ratio was maintained across treatments. Statistical models included linear model (LM), quadratic polynomial (QP), broken-line linear (BLL), and broken-line quadratic (BLQ). In Exp. 1, increasing STTD P increased (linear, P < 0.001) ADG, ADFI, G:F, final BW, and grams of STTD P intake per day and per kilogram of gain. There was also a marginal quadratic response for G:F (P < 0.066). In Exp. 2, ADG and G:F increased quadratically (P < 0.05), whereas ADFI increased linearly (P = 0.060) with increasing STTD P. The BLL and QP model provided similar fit to G:F in Exp. 1, estimating the requirement for maximum G:F at 0.34% and 0.42%, respectively. The BLL was the best fitting model for ADG and G:F in Exp. 2, estimating the breakpoint at 0.40% and 0.37% STTD P, respectively. The BLL and BLQ models estimated the breakpoint for ADG as a function of STTD P intake in grams per day at 2.92 and 3.02 g/d, respectively. These data provide empirical evidence that for 11- to 23-kg pigs, the NRC (2012) accurately estimates the STTD P requirement on a g/d basis. As a percentage of the diet, the STTD P requirement for diets without or with 1,000 FYT added phytase ranged from 0.34% to 0.42%.


2018 ◽  
Vol 2 (2) ◽  
pp. 169-183 ◽  
Author(s):  
Pedro E Urriola ◽  
Joey A Mielke ◽  
Qingqing Mao ◽  
Yuan-Tai Hung ◽  
John F Kurtz ◽  
...  

Abstract Although microalgae can be used as a source of energy and macronutrients in pig diets, there is limited information on the use of partially de-oiled microalgae coproducts in swine feeding programs. The objectives of this study were to evaluate the effects of a partially de-oiled microalgae extract (MAE) in nursery pig diets on growth performance and health status. A total of 300 pigs (initial BW = 6.3 ± 2.1 kg) were used in a 42-d experiment. Treatments included a standard corn-soybean meal control diet, and diets containing 1, 5, 10, or 20% MAE replacing primarily corn. The ME content of MAE was calculated from the chemical composition, and diets were formulated to meet or exceed nutrient requirements for nursery pigs. Pigs were stratified by weaning BW into 12 blocks in a randomized complete block design, with sex distributed evenly among blocks. Pens of pigs (5 pigs/pen) were assigned randomly within block to one of five dietary treatments. Pig BW and feed disappearance were recorded weekly. On day 42, 30 pigs were harvested and sections of the jejunum and ileum were collected for gut morphology analysis, and a liver sample was collected for metabolomic analysis using liquid chromatography-mass spectroscopy. Data were analyzed by ANOVA with diet as treatment effect, and contrasts were used to test linear or quadratic effects of dietary MAE inclusion level. Overall, pigs fed 1% and 5% MAE had the greatest (quadratic P &lt; 0.05) ADG, resulting from greater (quadratic P &lt; 0.05) ADFI. There was a tendency for a greater number of pigs requiring injectable treatments (P = 0.16) and a greater mortality (P = 0.14) in pigs fed the control diet than pigs in any of the diets with the MAE. Final BW increased (P &lt; 0.05) for pigs fed 1% and 5% MAE diets. The improvements in ADG were not explained by differences in mucosa height or goblet cell count among dietary treatments. Pigs fed diets containing 1% or 5% MAE had relatively less concentration (P &lt; 0.05) of ammonia in the liver and had changes in metabolites associated with the urea cycle. In conclusion, feeding MAE resulted in increased growth responses and may have beneficial health effects when fed to nursery pigs.


Author(s):  
Danung Nur Adli ◽  
Osfar Sjofjan

The aim of this study was to assess the effects of dietary probiotic enhanced liquid acidifier combined with mannan-rich fraction on growth performance, nutrients digestibility in growing pigs. Thirty [(Duroc×Yorkshire)×Landrace] pigs with the average initial BW of 36.75±1.57 kg were allocated into three treatments by a randomized complete block design. There were five pens per treatment with six pigs per pen. Dietary treatments include: 1) CON (basal diet); 2) T1 (basal diet+probiotic 0.1%) and 3) T2 (basal diet+probiotic+mannan rich fraction 0.2%). the data were analyzed as a randomized complete block design using of SAS University Version 4.0. The model included the effects of block (replication) and treatment. Pen served as the experimental unit. During the entire experimental period of 6 weeks, results showed that addition of complex probiotic at the level of 0.2% to diet increased ADG significantly (p<0.05). Also, digestibility of DM and N tended to increase. To sum up, results in this experiment indicated that dietary [(probiotik×acidifier)×mannan-rich-fraction]  supplementation had a positive effect on growing pigs performance and nutrient digestibility


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 179-180
Author(s):  
Jorge Y Perez-Palencia ◽  
Xixi Chen ◽  
Jirong Lv ◽  
Maíra Resende ◽  
Rhuan F Chaves ◽  
...  

Abstract The stressful events associated with weaning predispose piglets to reduced feed intake, which in concert with immature digestive and immune systems, results in increased incidences of diarrhea and poor performance. Flavors and sweeteners can be used in diets for young pigs as a means of attracting them to dry feed and increase their feed intake and performance after weaning. The objective of this study was to evaluate the effects of dietary inclusion of flavors and sweeteners and their combination on growth performance and post-weaning gut health of nursery pigs. A total of 1,144 weaned pigs (initial BW 6.61 ± 0.2 kg) were used in a randomized complete block design and assigned to one of four dietary treatments, with 11 replicates (pens) of 26 piglets per pen. Dietary treatments were 1) Control: standard 4-phase nursery feeding program; 2) Sweetener: control plus sweetener (0.015%); 3) Flavor: control plus flavoring (0.05%); 4) Combination: control plus sweetener and flavoring. Experimental diets were provided at a budget of 0.9, 3.4, 4.5, and 24 kg/pig in Phase 1, 2, 3, and 4, respectively. A common phase 5 diet was provided ad libitum until d56 after weaning. Feed disappearance and body weight were measured every other week. A pen fecal score assessment was performed at d4, 7, 10, and 14 after weaning. Overall, there were no differences among dietary treatments for growth performance. However, during the first two weeks post-weaning pigs fed diets containing flavors and sweeteners had a greater (P = 0.097) ADFI than control pigs. On d14, pigs fed diets containing flavors and sweeteners had less incidence of soft and watery feces (χ2 &lt; 0.05) compared to control pigs. This study provides evidence that dietary inclusion of flavors and sweeteners has important benefits to the overall health of nursery pigs likely related to feed intake in the period immediately after weaning.


2020 ◽  
Vol 98 (5) ◽  
Author(s):  
Lori L Thomas ◽  
Charmaine D Espinosa ◽  
Robert D Goodband ◽  
Hans H Stein ◽  
Mike D Tokach ◽  
...  

Abstract Five experiments were conducted to determine the standardized total tract digestibility (STTD) of P, digestible energy (DE), metabolizable energy (ME), and standardized ileal digestibility (SID) of amino acids (AA) in three sorghum varieties compared with corn and to determine the effects of sorghum varieties on nursery pig growth. In exp. 1, 48 barrows (initially 18.6 kg) were housed individually in metabolism crates. Treatments were arranged in a 2 × 4 factorial evaluating two levels of microbial phytase (0 or 500 units/kg) and four grain sources (corn, high-lysine, red, or white sorghum). Added phytase improved (P &lt; 0.05) STTD of P in all ingredients, but was not different among the grains. In exp. 2, the DE and ME in the three sorghum varieties were not different from corn. In exp. 3, 10 growing barrows (initially 25.9 kg) with a T-cannula in the terminal ileum were used. Standardized ileal digestible Lys, Met, Thr, and Val were greater (P &lt; 0.05) in corn than in the sorghum-based diets with no differences among the sorghum varieties. In exp. 4, 160 pigs (initially 6.3 kg) were randomly allotted to one of four dietary treatments with five pigs per pen and eight replicate pens per treatment in a 20-d experiment. Dietary treatments included corn or the three sorghum varieties, where the varieties of sorghum replaced corn on an SID Lys basis. No differences among treatments were observed in any growth performance parameters. In exp. 5, treatments consisted of a corn-based diet, a diet based on conventional sorghum (a mixture of red and white sorghum), and four diets with high-lysine sorghum containing increasing amounts of feed-grade AA, replacing soybean meal. Overall, pigs fed the high-lysine sorghum diet with the greatest amount of added feed-grade AA had the poorest gain:feed ratio (G:F; P &lt; 0.05) compared with pigs fed all the other experimental diets. Within those fed the high-lysine sorghum and feed-grade AA, average daily gain, final body weight (linear, P &lt; 0.10), and G:F (linear, P &lt; 0.01) decreased as feed-grade AA increased. In summary, no differences in STTD of P or in DE and ME were observed among the grain sources. The SID AA values for the three sorghum varieties were not different; however, they were all lower than for corn. These results indicate that these varieties of sorghum can successfully replace corn in nursery pig diets if diets are formulated to account for differences in AA digestibility.


2016 ◽  
Vol 96 (4) ◽  
pp. 563-569 ◽  
Author(s):  
F. Zhang ◽  
D. Ragland ◽  
O. Adeola

Two experiments were conducted to compare apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of calcium (Ca) in pigs. In experiment 1, three semipurified diets with soybean meal, canola meal, or sunflower meal as the sole source of Ca were formulated. Eighteen cannulated pigs (initial BW = 66 ± 5 kg) were assigned in a randomized complete block design to three treatments and six replicates per treatment. Results indicated that for either Ca or phosphorus (P), the ATTD was not different from the AID in the three diets (Psite = 0.16 and 0.81, respectively). In experiment 2, diets with four Ca concentrations were formulated with calcium carbonate as the Ca source. Sixteen cannulated pigs (initial BW = 73 ± 4 kg) were assigned in a randomized complete block design to four treatments in two experimental periods. The results indicated that the ATTD of Ca was not different from the AID for all diets. Furthermore, the Ca digestibility was not affected by dietary treatments, but the P digestibility was linearly improved (P < 0.01) as dietary Ca and P concentrations increased. Thus, it is concluded that both AID and ATTD can be used to describe the digestibility of Ca for growing–finishing pigs.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 181-181
Author(s):  
Brittany Carrender ◽  
Hayden E Williams ◽  
Mandy Gerhart ◽  
Kyle Coble ◽  
Joel M DeRouchey ◽  
...  

Abstract A total of 2,673 mixed sex pigs (DNA 600 × PIC 1050; initially 21.4 kg BW) were used in a 117-d study to evaluate the effects of increasing SID Lysine:Calorie NE ratio on the growth performance and carcass characteristics of DNA sired pigs. Pens of pigs (30 pigs/pen) were blocked by BW and location and allotted to 1 of 4 dietary treatments with 18 pens per treatment. Treatments were 90%, 95%, 100%, or 105% of the 2016 PIC recommended SID Lysine:Calorie NE ratio for finishing gilts. Data were analyzed as a randomized complete-block design with pen as the experimental unit. Overall (d 0-117), increasing Lysine:Calorie NE tended to improve ADG (Linear; P = 0.077) and G:F (Linear; P = 0.053). Although there was an increase in ADG with increasing SID Lysine:Calorie NE, there was no evidence for a difference (P &gt; 0.100) in final BW. The lack of BW response is most likely due to the lower (Quadratic; P &lt; 0.008) removal rate as SID Lysine:Calorie NE increased. Carcass ADG (Linear; P &lt; 0.064) tended to improve as SID Lysine: Calorie NE increased. An improvement in carcass G:F (Quadratic; P &lt; 0.004) was observed as SID Lysine:Calorie NE increased to 100% PIC (2016) recommendations, with little improvement observed thereafter. No significant differences were observed in carcass yield, loin depth, back fat, or % lean. These results suggests that DNA sired pigs fed increasing SID Lysine:Calorie NE ratios tended to have improved growth performance with no impact on carcass quality.


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